表皮生长因子、荧光素偶联物(荧光素 EGF)
表皮生长因子、荧光素偶联物(荧光素 EGF)
Invitrogen™

表皮生长因子、荧光素偶联物(荧光素 EGF)

我们提供了几种表皮生长因子偶联物,这些偶联物对于检测细胞中的 EGF 受体非常有用。荧光标记的 EGF 使科学家能够考察受体-膜之间的相互作用,研究受体分布,计算 EGF 与受体相互作用的速率常数等。该偶联物含有荧光素 (FITC)了解更多信息
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货号数量
E347820 μg
货号 E3478
价格(CNY)
7,356.00
Each
添加至购物车
数量:
20 μg
价格(CNY)
7,356.00
Each
添加至购物车
我们提供了几种表皮生长因子偶联物,这些偶联物对于检测细胞中的 EGF 受体非常有用。荧光标记的 EGF 使科学家能够考察受体-膜之间的相互作用,研究受体分布,计算 EGF 与受体相互作用的速率常数等。该偶联物含有荧光素 (FITC)、Oregon Green™ 514 和四甲基罗丹明,使用与 EGF 直接连接的染料构建。EGF 与 Alexa Fluor™ 染料和 Texas Red™ 染料的偶联物是染料–链霉亲和素分子和生物素化 EGF 的复合物。生物素化偶联物利用生物素-XX,它含有一个长间隔臂,旨在增强探针对 EGF 受体的亲和力。

表皮生长因子偶联物规格:
• EGF 分子:53 个氨基酸,MW=6,045 Da
• 标记(激发/发射波长):荧光素 (∼494/518 nm)
• 每个 EGF 分子上的荧光基团分子数量:1 个
•通常使用流式细胞仪、荧光显微镜或荧光计监测荧光信号


查找更多的受体结合和吞噬作用探针
我们提供多种用于研究受体介导的内吞作用的荧光标记探针,用于内吞作用和胞吐作用的膜标记物,以及检测内吞的荧光配体的方法。查看 Molecular Probes™ 手册中用于追踪受体结合和吞噬作用的探针—第 16.1 节,了解有关这些产品的更多信息。

供研究使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
标签或染料经典染料
数量20 μg
运输条件湿冰
形式固体
产品类型表皮生长因子
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

引用和文献 (18)

引用和文献
Abstract
Visualization of epidermal growth factor (EGF) receptor aggregation in plasma membranes by fluorescence resonance energy transfer. Correlation of receptor activation with aggregation.
Authors:Carraway KL, Koland JG, Cerione RA
Journal:J Biol Chem
PubMed ID:2498317
'Fluorescence resonance energy transfer between epidermal growth factor (EGF) molecules, labeled with fluorescent reporter groups, was used as a monitor for EGF receptor-receptor interactions in plasma membranes isolated from human epidermoid A431 cells. Epidermal growth factor molecules labeled at the amino terminus with fluorescein isothiocyanate served as donor molecules in ... More
The interaction of epidermal growth factor with its receptor in A431 cell membranes: a stopped-flow fluorescence anisotropy study.
Authors:Rousseau DL, Staros JV, Beechem JM
Journal:Biochemistry
PubMed ID:7578056
'We describe a quantitative examination of the interaction of epidermal growth factor (EGF) with the EGF receptor using A431 cell membrane vesicles as a receptor source. Using T-format steady-state fluorescence anisotropy detection coupled with stopped-flow mixing, we measured the association and EGF-induced dissociation kinetics of fluorescein 5-isothiocyanate-labeled mEGF (FITC-EGF) with ... More
Electric field-directed cell motility involves up-regulated expression and asymmetric redistribution of the epidermal growth factor receptors and is enhanced by fibronectin and laminin.
Authors:Zhao M, Dick A, Forrester JV, McCaig CD
Journal:Mol Biol Cell
PubMed ID:10198071
'Wounding corneal epithelium establishes a laterally oriented, DC electric field (EF). Corneal epithelial cells (CECs) cultured in similar physiological EFs migrate cathodally, but this requires serum growth factors. Migration depends also on the substrate. On fibronectin (FN) or laminin (LAM) substrates in EF, cells migrated faster and more directly cathodally. ... More
Heterogeneity of epidermal growth factor binding kinetics on individual cells.
Authors:Chung JC, Sciaky N, Gross DJ
Journal:Biophys J
PubMed ID:9251825
'Binding of fluorescein-conjugated epidermal growth factor (EGF) to individual A431 cells at 4 degrees C is measured by a quantitative fluorescence imaging technique. After background fluorescence and cell autofluorescence photobleaching corrections, the kinetic data are fit to simple models of one monovalent site and two independent monovalent sites, both of ... More
c-Jun N-terminal kinase 2 (JNK2) enhances cell migration through epidermal growth factor substrate 8 (EPS8).
Authors:Mitra S, Lee JS, Cantrell M, Van den Berg CL,
Journal:J Biol Chem
PubMed ID:21357683
Membrane-bound receptors induce biochemical signals to remodel the actin cytoskeleton and mediate cell motility. In association with receptor tyrosine kinases, several downstream mitogen-induced kinases facilitate cell migration. Here, we show a role for c-Jun N-terminal kinase 2 (JNK2) in promoting mammary cancer cell migration through inhibition of epidermal growth factor ... More