Differential effects of deuterium oxide on the fluorescence lifetimes and intensities of dyes with different modes of binding to DNA.
AuthorsSailer BL, Nastasi AJ, Valdez JG, Steinkamp JA, Crissman HA
JournalJ Histochem Cytochem
PubMed ID9016307
'Deuterium oxide (D2O) increases both the fluorescence lifetime and the fluorescence intensity of the intercalating dyes propidium iodide (PI) and ethidium bromide (EB) when bound to nucleic acid structures. We have used spectroscopic analysis coupled with conventional and phase-sensitive flow cytometry to compare the alterations in intensity and lifetime of ... More
Diverse microglial motility behaviors during clearance of dead cells in hippocampal slices.
AuthorsPetersen MA, Dailey ME
JournalGlia
PubMed ID15042586
'We used two-channel three-dimensional time-lapse fluorescence confocal imaging in live rat hippocampal slice cultures (1-7 days in vitro) to determine the motility behaviors of activated microglia as they engage dead and dying cells following traumatic brain tissue injury. Live microglia were labeled with a fluorescently conjugated lectin (IB(4)), and dead ... More
Calmodulin antagonists differentially affect capacitation-associated protein tyrosine phosphorylation of mouse sperm components.
AuthorsZeng HT, Tulsiani DR
JournalJ Cell Sci
PubMed ID12668727
'Sperm capacitation in vitro is thought to be correlated with the increased protein tyrosine phosphorylation of a subset of sperm components. Our group recently used a pharmacological approach to demonstrate that calmodulin (CaM), a 17 kDa calcium sensor protein, has a role in sperm capacitation. In the present study, we ... More
Automated image analysis of live/dead staining of the fungus Aureobasidium pullulans on microscope slides and leaf surfaces.
AuthorsNelson CD, Spear RN, Andrews JH
JournalBiotechniques
PubMed ID11056819
'An image analysis program and protocol for the identification and enumeration of live versus dead cells of the yeast-like fungus Aureobasidium pullulans was developed for both populations on microscope slides and leaf surfaces. Live cells took up CellTracker Blue, while nonviable cells stained with DEAD Red. Image analysis macro programs ... More
A2E-epoxides damage DNA in retinal pigment epithelial cells. Vitamin E and other antioxidants inhibit A2E-epoxide formation.
AuthorsSparrow JR, Vollmer-Snarr HR, Zhou J, Jang YP, Jockusch S, Itagaki Y, Nakanishi K
JournalJ Biol Chem
PubMed ID12646558
'The autofluorescent pigments that accumulate in retinal pigment epithelial cells with aging and in some retinal disorders have been implicated in the etiology of macular degeneration. The major constituent is the fluorophore A2E, a pyridinium bisretinoid. Light-exposed A2E-laden retinal pigment epithelium exhibits a propensity for apoptosis with light in the ... More
Polarization of scatter and fluorescence signals in flow cytometry.
AuthorsAsbury CL, Uy JL, van den Engh G
JournalCytometry
PubMed ID10805928
'BACKGROUND: The pulses of light scatter and fluorescence measured in flow cytometers exhibit varying degrees of polarization. Flow cytometers are heterogeneously sensitive to this polarization, depending on the light source(s), the optical layout, and the types of mirrors and filters used. Therefore, fluorescence polarization can affect apparent intensity ratios between ... More
The MspA porin promotes growth and increases antibiotic susceptibility of both Mycobacterium bovis BCG and Mycobacterium tuberculosis.
AuthorsMailaender C, Reiling N, Engelhardt H, Bossmann S, Ehlers S, Niederweis M
JournalMicrobiology
PubMed ID15073295
'Porins mediate the diffusion of hydrophilic solutes across the outer membrane of mycobacteria, but the efficiency of this pathway is very low compared to Gram-negative bacteria. To examine the importance of porins in slow-growing mycobacteria, the major porin MspA of Mycobacterium smegmatis was expressed in Mycobacterium tuberculosis and Mycobacterium bovis. ... More
Airway epithelial integrity is protected by a long-acting beta2-adrenergic receptor agonist.
AuthorsCoraux C, Kileztky C, Polette M, Hinnrasky J, Zahm JM, Devillier P, De Bentzmann S, Puchelle E
JournalAm J Respir Cell Mol Biol
PubMed ID14527924
'Airway epithelial integrity may be impaired by bacterial exoproducts, which are able to degrade tight junction-associated proteins such as zonula occludens 1 (ZO-1). We have investigated the protective effect of salmeterol, a long-acting beta(2)-adrenergic agonist, on Pseudomonas aeruginosa-induced alteration of the epithelial junctional barrier. We demonstrate in human airway epithelial ... More
R5 HIV productively infects Langerhans cells, and infection levels are regulated by compound CCR5 polymorphisms.
AuthorsKawamura T, Gulden FO, Sugaya M, McNamara DT, Borris DL, Lederman MM, Orenstein JM, Zimmerman PA, Blauvelt A
JournalProc Natl Acad Sci U S A
PubMed ID12815099
'Langerhans cells (LCs) are suspected to be initial targets for HIV after sexual exposure (by becoming infected or by capturing virus). Here, productive R5 HIV infection of LC ex vivo and LC-mediated transmission of virus to CD4+ T cells were both found to depend on CCR5. By contrast, infection of ... More
A new and simple method to evaluate early membrane changes in frozen-thawed boar spermatozoa.
AuthorsPeña FJ, Saravia F, Johannisson A, Walgren M, Rodríguez-Martínez H
JournalInt J Androl
PubMed ID15811072
'Detection of early changes in the sperm plasma membrane during cryopreservation is of utmost importance when designing freezing protocols and has previously been studied in the pig species using annexin-V detection of phosphatidylserine translocation. In the present study we designed a new assay to detect these changes in boar spermatozoa, ... More
Separation of nucleic acids by capillary electrophoresis in cellulose solutions with mono- and bis-intercalating dyes.
AuthorsKim Y, Morris MD
JournalAnal Chem
PubMed ID8160960
'The effects of bis- and mono-intercalating dyes on the capillary electrophoretic separation of double-stranded DNA have been investigated in buffers containing (hydroxypropylmethyl)-cellulose. Broad bands and incomplete separations of phi X 174 DNA HaeIII digest were obtained with the bis-intercalators ethidium homodimer 1 (EthD-1) and ethidium homodimer 2 (EthD-2), under a ... More
Structural insights and biological effects of glycogen synthase kinase 3-specific inhibitor AR-A014418.
AuthorsBhat R, Xue Y, Berg S, Hellberg S, Ormö M, Nilsson Y, Radesäter AC, Jerning E, Markgren PO, Borgegård T, Nylöf M, Giménez-Cassina A, Hernández F, Lucas JJ, Díaz-Nido J, Avila J
JournalJ Biol Chem
PubMed ID12928438
'Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that has been implicated in pathological conditions such as diabetes and Alzheimer''s disease. We report the characterization of a GSK3 inhibitor, AR-A014418, which inhibits GSK3 (IC50 = 104 +/- 27 nM), in an ATP-competitive manner (Ki = 38 nM). AR-A014418 does ... More
Decreased stimulation of CD4+ T cell proliferation and IL-2 production by highly enriched populations of HIV-infected dendritic cells.
AuthorsKawamura T, Gatanaga H, Borris DL, Connors M, Mitsuya H, Blauvelt A
JournalJ Immunol
PubMed ID12682260
'APC infection and dysfunction may contribute to the immunopathogenesis of HIV disease. In this study, we examined immunologic function of highly enriched populations of HIV-infected monocyte-derived dendritic cells (DC). Compared with uninfected DC, HIV-infected DC markedly down-regulated surface expression of CD4. HIV p24(+) DC were then enriched by negative selection ... More
Ontogeny of central CO2 chemoreception: chemosensitivity in the ventral medulla of developing bullfrogs.
AuthorsTaylor BE, Harris MB, Leiter JC, Gdovin MJ
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID14615406
'Sites of central CO2 chemosensitivity were investigated in isolated brain stems from Rana catesbeiana tadpoles and frogs. Respiratory neurograms were made from cranial nerve (CN) 7 and spinal nerve 2. Superfusion of the brain stem with hypercapnic artificial cerebrospinal fluid elicited increased fictive lung ventilation. The effect of focal perfusion ... More
Detection of duck hepatitis B virus DNA fragments using on-column intercalating dye labeling with capillary electrophoresis-laser-induced fluorescence.
AuthorsTan WG, Tyrrell DL, Dovichi NJ
JournalJ Chromatogr A
PubMed ID10486738
'A rapid on-column DNA labeling technique is used to detect viral restriction DNA fragments by capillary electrophoresis-laser induced fluorescence detection. Intercalating dyes such as POPO3 or ethidium homodimer-2 are incorporated into the detection buffer. The cationic dyes migrate into the capillary during electrophoresis and bind to the oppositely migrating DNA ... More
Anaerobic nitrogen-fixing consortia consisting of clostridia isolated from gramineous plants.
AuthorsMinamisawa K, Nishioka K, Miyaki T, Ye B, Miyamoto T, You M, Saito A, Saito M, Barraquio WL, Teaumroong N, Sein T, Sato T
JournalAppl Environ Microbiol
PubMed ID15128572
'We report here the existence of anaerobic nitrogen-fixing consortia (ANFICOs) consisting of N(2)-fixing clostridia and diverse nondiazotrophic bacteria in nonleguminous plants; we found these ANFICOs while attempting to overcome a problem with culturing nitrogen-fixing microbes from various gramineous plants. A major feature of ANFICOs is that N(2) fixation by the ... More
The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria.
AuthorsGuy R, Liu P, Pennefather P, Crandall I,
JournalMalar J
PubMed ID17617912
'BACKGROUND: Giemsa staining of thick blood smears remains the "gold standard" for detecting malaria. However, this method is not very good for diagnosing low-level infections. A method for the simultaneous staining of Plasmodium-parasitized culture and blood smears for both bright field and fluorescence was developed and its ability to improve ... More
PSC-RANTES blocks R5 human immunodeficiency virus infection of Langerhans cells isolated from individuals with a variety of CCR5 diplotypes.
AuthorsKawamura T, Bruse SE, Abraha A, Sugaya M, Hartley O, Offord RE, Arts EJ, Zimmerman PA, Blauvelt A, Bruce SE
JournalJ Virol
PubMed ID15220435
'Topical microbicides that effectively block interactions between CCR5(+) immature Langerhans cells (LC) residing within genital epithelia and R5 human immunodeficiency virus (HIV) may decrease sexual transmission of HIV. Here, we investigated the ability of synthetic RANTES analogues (AOP-, NNY-, and PSC-RANTES) to block R5 HIV infection of human immature LC ... More
Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes.
AuthorsPozharski E,
JournalMethods Mol Biol
PubMed ID20013410
Lipoplex preparations are heterogeneous mixtures of lipoplex particles of different structures. As these structures determine the efficiency of the delivery of genetic material, it is important to characterize the distribution of particles of different types in lipoplex preparations with good statistics. We describe the application of flow fluorometry which allows ... More
Modulation of mammalian life span by the short isoform of p53.
AuthorsMaier B, Gluba W, Bernier B, Turner T, Mohammad K, Guise T, Sutherland A, Thorner M, Scrable H
JournalGenes Dev
PubMed ID14871929
Overexpression of the short isoform of p53 (p44) has unexpectedly uncovered a role for p53 in the regulation of size and life span in the mouse. Hyperactivation of the insulin-like growth factor (IGF) signaling axis by p44 sets in motion a kinase cascade that clamps potentially unimpeded growth through p21Cip1. ... More
Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA.
AuthorsKoynova R, Tarahovsky YS, Wang L, MacDonald RC,
JournalBiochim Biophys Acta
PubMed ID17156744
Lipoplexes containing a mixture of cationic phospholipids dioleoylethylphosphatidylcholine (EDOPC) and dilauroylethylphosphatidylcholine (EDLPC) are known to be far more efficient agents in transfection of cultured primary endothelial cells than are lipoplexes containing either lipid alone. The large magnitude of the synergy permits comparison of the physical and physico-chemical properties of lipoplexes ... More
Analysis of the structure and composition of individual lipoplex particles by flow fluorometry.
AuthorsPozharski EV, Macdonald RC
JournalAnal Biochem
PubMed ID15907868
A flow fluorometric approach to study cationic lipoid-DNA complexes is presented. The approach uses standard flow cytometry equipment and common fluorescent dyes (BODIPY and ethidium homodimer-2) to detect both lipoid and DNA content in individual particles. In addition, a procedure that allows determination of whether or not liposomes remain intact ... More
Ventilatory effects of glial dysfunction in a rat brain stem chemoreceptor region.
AuthorsErlichman JS, Li A, Nattie EE
JournalJ Appl Physiol
PubMed ID9804558
Glia are thought to be important in brain extracellular fluid ion and pH regulation, but their role in brain stem sites that sense pH and stimulate breathing is unknown. Using a diffusion pipette, we administered the glial toxin, fluorocitrate (FC; 1 mM) into one such brain stem region, the retrotrapezoid ... More
Isolation of living neurons from human elderly brains using the immunomagnetic sorting DNA-linker system.
AuthorsKonishi Y, Lindholm K, Yang LB, Li R, Shen Y
JournalAm J Pathol
PubMed ID12414505
Isolation and culture of mature neurons from affected brain regions during diseased states provide a well-suited in vitro model system to study age-related neurodegeneration under dynamic conditions at cellular levels. We have developed a novel technique to isolate living neurons from rapidly autopsied human elderly brains, and have succeeded in ... More
Microbial characterization of biofilms in domestic drains and the establishment of stable biofilm microcosms.
AuthorsMcBain AJ, Bartolo RG, Catrenich CE, Charbonneau D, Ledder RG, Rickard AH, Symmons SA, Gilbert P
JournalAppl Environ Microbiol
PubMed ID12513993
We have used heterotrophic plate counts, together with live-dead direct staining and denaturing gradient gel electrophoresis (DGGE), to characterize the eubacterial communities that had formed as biofilms within domestic sink drain outlets. Laboratory microcosms of these environments were established using excised biofilms from two separate drain biofilm samples to inoculate ... More
Frequency, size, and localization of bacterial aggregates on bean leaf surfaces.
AuthorsMonier JM, Lindow SE
JournalAppl Environ Microbiol
PubMed ID14711662
Using epifluorescence microscopy and image analysis, we have quantitatively described the frequency, size, and spatial distribution of bacterial aggregates on leaf surfaces of greenhouse-grown bean plants inoculated with the plant-pathogenic bacterium Pseudomonas syringae pv. syringae strain B728a. Bacterial cells were not randomly distributed on the leaf surface but occurred in ... More
Identification of a domain within the multifunctional Vibrio cholerae RTX toxin that covalently cross-links actin.
AuthorsSheahan KL, Cordero CL, Satchell KJ
JournalProc Natl Acad Sci U S A
PubMed ID15199181
The Gram-negative pathogen Vibrio cholerae causes diarrheal disease through the export of enterotoxins. The V. cholerae RTX toxin was previously identified and characterized by its ability to round human laryngeal epithelial (HEp-2) cells. Further investigation determined that cell rounding is caused by the depolymerization of actin stress fibers, through the ... More
Cell death in Pseudomonas aeruginosa biofilm development.
AuthorsWebb JS, Thompson LS, James S, Charlton T, Tolker-Nielsen T, Koch B, Givskov M, Kjelleberg S
JournalJ Bacteriol
PubMed ID12867469
Bacteria growing in biofilms often develop multicellular, three-dimensional structures known as microcolonies. Complex differentiation within biofilms of Pseudomonas aeruginosa occurs, leading to the creation of voids inside microcolonies and to the dispersal of cells from within these voids. However, key developmental processes regulating these events are poorly understood. A normal ... More
Nitric oxide acts as an antioxidant and delays programmed cell death in barley aleurone layers.
AuthorsBeligni MV, Fath A, Bethke PC, Lamattina L, Jones RL
JournalPlant Physiol
PubMed ID12177477
Nitric oxide (NO) is a freely diffusible, gaseous free radical and an important signaling molecule in animals. In plants, NO influences aspects of growth and development, and can affect plant responses to stress. In some cases, the effects of NO are the result of its interaction with reactive oxygen species ... More
Validation of an in vitro screen for phospholipidosis using a high-content biology platform.
Several cationic amphiphilic drugs cause local or systemic phospholipidosis (PLD) after chronic exposure in preclinical species. PLD is characterized by the accumulation of drug, phospholipid, and concentric lamellar bodies in cellular lysosomes. We have developed a fluorescence-based in vitro screen that is predictive of PLD using the Cellomics ArrayScan high-content ... More
Viability assessment of turkey sperm using fluorescent staining and flow cytometry.
AuthorsDonoghue AM, Garner DL, Donoghue DJ, Johnson LA
JournalPoult Sci
PubMed ID7479495
Turkey sperm viability was evaluated using several fluorescent stains both singularly and in combination. Dilution curves and several extenders were used to determine optimal stain concentrations. Semen was collected from eight toms, pooled, diluted, stained, and evaluated microscopically within 2 h of collection. Replicates were assessed for both viable and ... More
HIV-infected Langerhans cells preferentially transmit virus to proliferating autologous CD4+ memory T cells located within Langerhans cell-T cell clusters.
AuthorsSugaya M, Loré K, Koup RA, Douek DC, Blauvelt A
JournalJ Immunol
PubMed ID14764689
Langerhans cells (LC) are likely initial targets for HIV following sexual exposure to virus and provide an efficient means for HIV to gain access to lymph node T cells. The purpose of this study was to examine the nature of the CD4(+) T cell that becomes infected by HIV-infected LC. ... More
Brain stem lesion size determined by DEAD red or conjugation of neurotoxin to fluorescent beads.
AuthorsNattie EE, Erlichman JS, Li A
JournalJ Appl Physiol
PubMed ID9843566
Neurotoxin microinjected into the retrotrapezoid nucleus of anesthetized rats decreases phrenic activity and eliminates the response to CO2. In unanesthetized rats, such treatment has no effect on awake, resting breathing and decreases CO2 sensitivity by 40% (M. Akilesh, M. Kamper, A. Li, and E. E. Nattie. J. Appl. Physiol. 82: ... More
Exposure of sink drain microcosms to triclosan: population dynamics and antimicrobial susceptibility.
Recent concern that the increased use of triclosan (TCS) in consumer products may contribute to the emergence of antibiotic resistance has led us to examine the effects of TCS dosing on domestic-drain biofilm microcosms. TCS-containing domestic detergent (TCSD) markedly lowered biofouling at 50% (wt/vol) but was poorly effective at use ... More
Effects of quaternary-ammonium-based formulations on bacterial community dynamics and antimicrobial susceptibility.
AuthorsMcBain AJ, Ledder RG, Moore LE, Catrenich CE, Gilbert P
JournalAppl Environ Microbiol
PubMed ID15184143
Quaternary ammonium compounds (QACs) are widely used as adjuncts to hygiene in domestic cleaning products. Current concern that the increased use of such biocides in consumer products might contribute to the emergence of antibiotic resistance has led us to examine the effects of a QAC-containing domestic cleaning fluid on the ... More
Longitudinal characterization of multispecies microbial populations recovered from spaceflight potable water.
Authors
JournalNPJ Biofilms Microbiomes
PubMed ID34489467
Investigation of The Cellular Response to Bone Fractures: Evidence for Flexoelectricity.
AuthorsNúñez-Toldrà R, Vasquez-Sancho F, Barroca N, Catalan G
JournalSci Rep
PubMed ID31937885
'The recent discovery of bone flexoelectricity (strain-gradient-induced electrical polarization) suggests that flexoelectricity could have physiological effects in bones, and specifically near bone fractures, where flexoelectricity is theoretically highest. Here, we report a cytological study of the interaction between crack stress and bone cells. We have cultured MC3T3-E1 mouse osteoblastic cells ... More
Transwell Insert-Embedded Microfluidic Devices for Time-Lapse Monitoring of Alveolar Epithelium Barrier Function under Various Stimulations.
Authors
JournalMicromachines (Basel)
PubMed ID33917518
Targeted genome-wide methylation and gene expression analyses reveal signaling pathways involved in ovarian dysfunction after developmental EDC exposure in rats.
Authors
JournalBiol Reprod
PubMed ID23303685
Protein tyrosine kinase signaling in the mouse oocyte cortex during sperm-egg interactions and anaphase resumption.
Authors
JournalMol Reprod Dev
PubMed ID23401167
Orthotopic transplantation of neonatal GFP rat ovary as experimental model to study ovarian development and toxicology.
Authors
JournalReprod Toxicol
PubMed ID18848623
Fetal and neonatal exposure to the endocrine disruptor methoxychlor causes epigenetic alterations in adult ovarian genes.
Authors
JournalEndocrinology
PubMed ID19589859
Effect of ambient temperature and intracellular pigmentation on photothermal damage rate kinetics.
Authors
JournalJ Biomed Opt
PubMed ID31230427
Development of quantitative microscopy-based assays for evaluating dynamics of living cultures of mouse spermatogonial stem/progenitor cells.
Authors
JournalBiol Reprod
PubMed ID22933516
Schwann cell nodal membrane disruption triggers bystander axonal degeneration in a Guillain-Barré syndrome mouse model.
Authors
JournalJ Clin Invest
PubMed ID35671105
Quercetin hinders microglial activation to alleviate neurotoxicity via the interplay between NLRP3 inflammasome and mitophagy.
Authors
JournalRedox Biol
PubMed ID34082381
Frontotemporal Dementia-Associated N279K Tau Mutation Localizes at the Nuclear Compartment.
AuthorsRitter ML, Avila J, García-Escudero V, Hernández F, Pérez M
JournalFront Cell Neurosci
PubMed ID30050413
Tau is a microtubule-associated protein that plays an important role in Alzheimer's disease and related tauopathies. Approximately one-half of all cases of Frontotemporal dementia with parkinsonism-17 (FTDP-17) are caused by mutations in the MAPT gene. The N279K mutation is one of the three mutations more prevalent in FTDP-17 cases. Several ... More
Application of mild hypothermia successfully mitigates neural injury in a 3D in-vitro model of traumatic brain injury.
AuthorsScimone MT, Cramer HC, Hopkins P, Estrada JB, Franck C
JournalPLoS One
PubMed ID32236105
Therapeutic hypothermia (TH) is an attractive target for mild traumatic brain injury (mTBI) treatment, yet significant gaps in our mechanistic understanding of TH, especially at the cellular level, remain and need to be addressed for significant forward progress to be made. Using a recently-established 3D in-vitro neural hydrogel model for ... More
Targeted crystallization of mixed-charge nanoparticles in lysosomes induces selective death of cancer cells.
AuthorsBorkowska M, Siek M, Kolygina DV, Sobolev YI, Lach S, Kumar S, Cho YK, Kandere-Grzybowska K, Grzybowski BA
JournalNat Nanotechnol
PubMed ID32203435
Lysosomes have become an important target for anticancer therapeutics because lysosomal cell death bypasses the classical caspase-dependent apoptosis pathway, enabling the targeting of apoptosis- and drug-resistant cancers. However, only a few small molecules-mostly repurposed drugs-have been tested so far, and these typically exhibit low cancer selectivity, making them suitable only ... More
Nanogold Neuroprotection in Human Neural Stem Cells Against Amyloid-beta-induced Mitochondrial Dysfunction.
AuthorsChiang MC, Nicol CJB, Cheng YC, Yen C, Lin CH, Chen SJ, Huang RN
JournalNeuroscience
PubMed ID32229231
Alzheimer's disease (AD) is a neuronal dementia with progressive memory loss. Amyloid-beta (Aß) peptides has major effect in the neurodegenerative disorder, which are thought to promote mitochondrial dysfunction in AD brains. Anti-AD drugs acting upon the brain are generally difficult to develop, often cause serious side effects or lack therapeutic ... More
Integrin-specific hydrogels modulate transplanted human bone marrow-derived mesenchymal stem cell survival, engraftment, and reparative activities.
AuthorsClark AY, Martin KE, García JR, Johnson CT, Theriault HS, Han WM, Zhou DW, Botchwey EA, García AJ
JournalNat Commun
PubMed ID31913286
Stem cell therapies are limited by poor cell survival and engraftment. A hurdle to the use of materials for cell delivery is the lack of understanding of material properties that govern transplanted stem cell functionality. Here, we show that synthetic hydrogels presenting integrin-specific peptides enhance the survival, persistence, and osteo-reparative ... More
PARP1 blockade is synthetically lethal in XRCC1 deficient sporadic epithelial ovarian cancers.
AuthorsAli R, Alabdullah M, Alblihy A, Miligy I, Mesquita KA, Chan SY, Moseley P, Rakha EA, Madhusudan S
JournalCancer Lett
PubMed ID31669203
PARP1 inhibitor (Niraparib, Olaparib, Rucaparib) maintenance therapy improves progression-free survival in platinum sensitive sporadic epithelial ovarian cancers. However, biomarkers of response to PARPi therapy is yet to be clearly defined. XRCC1, a scaffolding protein, interacts with PARP1 during BER and SSBR. In a large clinical cohort of 525 sporadic ovarian ... More