Enzymatic activity of alkaline phosphatase inside protein and polymer structures fabricated via multiphoton excitation.
AuthorsBasu S, Campagnola PJ
JournalBiomacromolecules
PubMed ID15003023
'We demonstrate micron scale control of bioactivity through the use of multiphoton excited photochemistry, where this technique has been used to cross-link three-dimensional matrixes of alkaline phosphatase, bovine serum albumin, and polyacrylamide and combinations therein. Using a fluorescence-based assay (ELF-97), the enzymatic activity has been studied using a Michaelis-Menten analysis, ... More