ELF™ 97 磷酸酶底物（ELF™97 磷酸盐），0.2 μm 过滤
ELF™ 97 磷酸酶底物（ELF™97 磷酸盐），0.2 μm 过滤
引用和文献 (38)
Invitrogen™

ELF™ 97 磷酸酶底物（ELF™97 磷酸盐），0.2 μm 过滤

ELF™ 97 磷酸酶底物水解后在酶活性位点产生明亮和光稳定的黄绿色荧光沉淀物。这种荧光沉淀物有几个独特的光谱特征，包括一个非常大的斯托克位移，这使得它很容易与内生荧光区分。了解更多信息
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货号数量
E65881 mL
货号 E6588
价格（CNY)
5,153.00
飞享价
Ends: 31-Dec-2025
6,985.00
共减 1,832.00 (26%)
Each
添加至购物车
数量:
1 mL
价格（CNY)
5,153.00
飞享价
Ends: 31-Dec-2025
6,985.00
共减 1,832.00 (26%)
Each
添加至购物车
ELF™ 97 磷酸酶底物水解后在酶活性位点产生明亮和光稳定的黄绿色荧光沉淀物。这种荧光沉淀物有几个独特的光谱特征，包括一个非常大的斯托克位移，这使得它很容易与内生荧光区分。
仅供科研使用。不可用于诊断程序。
规格
细胞渗透性细胞通透性
颜色黄绿色
最大浓度5 mM
激发/发射345⁄530
适用于（设备）荧光显微镜、芯片、流式细胞仪
标签或染料ELF 97
产品线ELF
数量1 mL
研究类别分化
运输条件室温
底物磷酸酶底物
检测方法荧光
形式液体
底物属性化学底物
目标酶磷酸酶
Unit SizeEach
内容与储存
在冷冻冰箱（-5 至 -30°C）中避光储存。

常见问题解答 (FAQ)

Can the ELF 97 reagent be applied to live cells?

ELF 97 substrate is cell impermeant. It may be used on live cells that exhibit phosphatase activity on the surface of the cells, but not intracellular phosphatase activity in live cells. The alternative product for live-cell detection of phosphatase activity is the cell-permeable Alkaline Phosphatase Live Stain (Cat. No. A14353).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can ELF 97 stained cell/tissue samples be multiplexed with antibody labeling?

No. The ELF 97 reagent does not covalently attach to any cellular components and may be washed away with any subsequent antibody labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (38)

引用和文献
Abstract
Characterization of a new NIH-registered variant human embryonic stem cell line, BG01V: a tool for human embryonic stem cell research.
Authors:Plaia TW,Josephson R,Liu Y,Zeng X,Ording C,Toumadje A,Brimble SN,Sherrer ES,Uhl EW,Freed WJ,Schulz TC,Maitra A,Rao MS,Auerbach JM
Journal:Stem cells (Dayton, Ohio)
PubMed ID:16293579
Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.
Authors:Josephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM
Journal:Stem Cells
PubMed ID:17284651
'As the number of human embryonic stem cell (hESC) lines increases, so does the need for systematic evaluation of each line''s characteristics and potential. Comparisons between lines are complicated by variations in culture conditions, feeders, spontaneous differentiation, and the absence of standardized assays. These difficulties, combined with the inability of ... More
Quantitative differences in phase I and II metabolism between rat precision-cut liver slices and isolated hepatocytes.
Authors:Ekins S, Murray GI, Burke MD, Williams JA, Marchant NC, Hawksworth GM
Journal:Drug Metab Dispos
PubMed ID:8591730
'Testosterone (250 microM), 7-ethoxycoumarin (25 microM), and 1-chloro-2,4-dinitrobenzene (CDNB, 50 microM) were used as substrates to compare phase I and II metabolism in rat precision-cut liver slices and rat hepatocytes. Overall clearance to metabolites was significantly greater in hepatocytes for testosterone (1.9- to 16.9-fold), 7-ethoxycoumarin (O-deethylation, 14.8-fold; glucuronidation, 3.1-fold), and ... More
Alkaline phosphatase is involved in the control of adipogenesis in the murine preadipocyte cell line, 3T3-L1.
Authors:Ali AT, Penny CB, Paiker JE, van Niekerk C, Smit A, Ferris WF, Crowther NJ
Journal:Clin Chim Acta
PubMed ID:15748605
'OBJECTIVE: As alkaline phosphatase may play a role in cell differentiation, our aim was to study the possible role of this enzyme in the differentiation of preadipocytes (3T3-L1 cells) into adipocytes. RESEARCH METHODS AND PROCEDURES: 3T3-L1 cells were grown in medium containing insulin, dexamethasone and IBMX to induce adipogenesis. Adipogenesis ... More
Fluorescence in situ hybridization of scarce leptin receptor mRNA using the enzyme-labeled fluorescent substrate method and tyramide signal amplification.
Authors:Breininger JF, Baskin DG
Journal:J Histochem Cytochem
PubMed ID:11101627
'To increase the sensitivity of fluorescence in situ hybridization (FISH) for detection of low-abundance mRNAs, we performed FISH on cryostat sections of rat hypothalamus with biotin-labeled riboprobes to leptin receptor (ObRb) and amplified the signal by combining tyramide signal amplification (TSA) and Enzyme-Labeled Fluorescent alkaline phosphatase substrate (ELF) methods. First, ... More
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