ELF™ 97内源性磷酸酶检测试剂盒

引用和文献 (32)

Invitrogen™

ELF™ 97内源性磷酸酶检测试剂盒

ELF™ 97 内源性磷酸酶试剂盒可利用 ELF™ 97 荧光酶底物检测荧光酶活性；其水解后在酶活性位点产生明亮和光稳定的黄绿色荧光沉淀物。这种荧光沉淀物有几个独特的光谱特征，包括一个 180 nm 的超大斯托克位移，这使得它很容易与内生荧光区分了解更多信息

货号	数量
E6601	1 kit

货号 E6601

价格（CNY)

6,220.00

添加至购物车

1 kit

价格（CNY)

6,220.00

添加至购物车

ELF™ 97 内源性磷酸酶试剂盒可利用 ELF™ 97 荧光酶底物检测荧光酶活性；其水解后在酶活性位点产生明亮和光稳定的黄绿色荧光沉淀物。这种荧光沉淀物有几个独特的光谱特征，包括一个 180 nm 的超大斯托克位移，这使得它很容易与内生荧光区分。

仅供科研使用。不可用于诊断程序。

检测方法荧光

染料类型ELF 97

数量1 kit

运输条件湿冰

底物ELF™ 97 磷酸盐

底物属性化学底物

底物类型磷酸酶底物

目标酶磷酸酶

颜色黄绿色

发射345⁄530

适用于（应用）磷酸酶检测

适用于（设备）荧光显微镜

产品线ELF

产品类型磷酸酶检测

Unit SizeEach

内容与储存

在冰箱（2°C 至 8°C）中避光储存。

常见问题解答 (FAQ)

Can the ELF 97 reagent be applied to live cells?

ELF 97 substrate is cell impermeant. It may be used on live cells that exhibit phosphatase activity on the surface of the cells, but not intracellular phosphatase activity in live cells. The alternative product for live-cell detection of phosphatase activity is the cell-permeable Alkaline Phosphatase Live Stain (Cat. No. A14353).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can ELF 97 stained cell/tissue samples be multiplexed with antibody labeling?

No. The ELF 97 reagent does not covalently attach to any cellular components and may be washed away with any subsequent antibody labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (32)

引用和文献

Abstract

Characterization of a new NIH-registered variant human embryonic stem cell line, BG01V: a tool for human embryonic stem cell research.

Authors:Plaia TW,Josephson R,Liu Y,Zeng X,Ording C,Toumadje A,Brimble SN,Sherrer ES,Uhl EW,Freed WJ,Schulz TC,Maitra A,Rao MS,Auerbach JM

Journal:Stem cells (Dayton, Ohio)

PubMed ID:16293579

Primitive adult hematopoietic stem cells can function as osteoblast precursors.

Authors:Olmsted-Davis EA, Gugala Z, Camargo F, Gannon FH, Jackson K, Kienstra KA, Shine HD, Lindsey RW, Hirschi KK, Goodell MA, Brenner MK, Davis AR

Journal:Proc Natl Acad Sci U S A

PubMed ID:14673088

'Osteoblasts are continually recruited from stem cell pools to maintain bone. Although their immediate precursor is a plastic-adherent mesenchymal stem cell able to generate tissues other than bone, increasing evidence suggests the existence of a more primitive cell that can differentiate to both hematopoietic and mesenchymal cells. We show here ... More

Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.

Authors:Josephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM

Journal:Stem Cells

PubMed ID:17284651

'As the number of human embryonic stem cell (hESC) lines increases, so does the need for systematic evaluation of each line''s characteristics and potential. Comparisons between lines are complicated by variations in culture conditions, feeders, spontaneous differentiation, and the absence of standardized assays. These difficulties, combined with the inability of ... More

Osteoclast responses to lipopolysaccharide, parathyroid hormone and bisphosphonates in neonatal murine calvaria analyzed by laser scanning confocal microscopy.

Authors:Suzuki K, Takeyama S, Kikuchi T, Yamada S, Sodek J, Shinoda H

Journal:J Histochem Cytochem

PubMed ID:16087705

'Because the development and activity of osteoclasts in bone remodeling is critically dependent on cell-cell and cell-matrix interactions, we used laser confocal microscopy to study the response of osteoclasts to lipopolysaccharide (LPS; 10 microg/ml), parathyroid hormone (PTH; 10(-8) M), and bisphosphonates (BPs; 1-25 microM clodronate or 0.1-2.5 microM risedronate) in ... More

Fluorescence-based staining for tartrate-resistant acidic phosphatase (TRAP) in osteoclasts combined with other fluorescent dyes and protocols.

Authors:Filgueira L

Journal:J Histochem Cytochem

PubMed ID:14966208

Osteoclasts are the only bone-resorbing cells. In addition to other specific properties, osteoclasts are characterized by their expression of tartrate-resistant acidic phosphatase (TRAP), which is usually detected using a histochemical method for light microscopy. Using ELF97 phosphatase substrate, this study describes a new fluorescence-based method for TRAP detection. The fluorescence-based ... More

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