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引用和文献 (19)
Invitrogen™
EnzChek™ 蛋白酶检测试剂盒，绿色荧光，100-1000 次检测
EnzChek™ 蛋白酶检测试剂盒（绿色荧光）是一种快速、简单的直接荧光检测方法，可用于检测金属蛋白酶、丝氨酸蛋白酶、酸性蛋白酶和巯基蛋白酶。可使用荧光微孔板读数仪测量伴随出现的荧光强度增加，后者与蛋白酶活性成正比。查看我们完整的荧光微孔板检测产品线。•通用机制允许测量各种蛋白酶•可用于持续测量蛋白酶动力学•505/513了解更多信息
| 货号 | 数量 |
|---|---|
| E6638 | 1 Kit |
货号 E6638
价格（CNY)
6,641.00
Each
数量:
1 Kit
价格（CNY)
6,641.00
Each
EnzChek™ 蛋白酶检测试剂盒（绿色荧光）是一种快速、简单的直接荧光检测方法，可用于检测金属蛋白酶、丝氨酸蛋白酶、酸性蛋白酶和巯基蛋白酶。可使用荧光微孔板读数仪测量伴随出现的荧光强度增加，后者与蛋白酶活性成正比。
查看我们完整的荧光微孔板检测产品线。
•通用机制允许测量各种蛋白酶
•可用于持续测量蛋白酶动力学
•505/513 nm 的激发/发射波长允许使用标准荧光素 (FITC) 滤光片设置
EnzChek™ 蛋白酶测定试剂盒包含一种酪蛋白衍生物，后者已被对 pH 值不敏感的绿色荧光 BODIPY™ FL 染料广泛标记，从而导致荧光染料淬灭。蛋白酶催化水解会释放出具有强烈荧光的 BODIPY™ FL 染料标记多肽，可用来定量检测溶液中的蛋白酶活性。绿色荧光 BODIPY™ FL 染料的激发和发射光谱与荧光素类似。
查看我们完整的荧光微孔板检测产品线。
•通用机制允许测量各种蛋白酶
•可用于持续测量蛋白酶动力学
•505/513 nm 的激发/发射波长允许使用标准荧光素 (FITC) 滤光片设置
EnzChek™ 蛋白酶测定试剂盒包含一种酪蛋白衍生物，后者已被对 pH 值不敏感的绿色荧光 BODIPY™ FL 染料广泛标记，从而导致荧光染料淬灭。蛋白酶催化水解会释放出具有强烈荧光的 BODIPY™ FL 染料标记多肽，可用来定量检测溶液中的蛋白酶活性。绿色荧光 BODIPY™ FL 染料的激发和发射光谱与荧光素类似。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光强度
数量1 Kit
研究类别分化
运输条件室温
底物属性基于蛋白的底物
底物类型蛋白酶底物
目标酶蛋白酶
适用于（应用）蛋白酶检测
适用于（设备）荧光微孔板读数仪
产品线EnzChek
产品类型蛋白酶检测
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。
常见问题解答 (FAQ)
What is the optimal pH range for protease activity detection using the EnzChek Protease Assay Kit?
What is the shelf life for EnzChek Protease Assay kits (Cat. Nos. E6638, E6639)?
引用和文献 (19)
引用和文献
Abstract
Journal:
PubMed ID:10748021
Effective antiprotease-antibiotic treatment of experimental anthrax.
Journal:BMC Infect Dis
PubMed ID:15819985
'BACKGROUND: Inhalation anthrax is characterized by a systemic spread of the challenge agent, Bacillus anthracis. It causes severe damage, including multiple hemorrhagic lesions, to host tissues and organs. It is widely believed that anthrax lethal toxin secreted by proliferating bacteria is a major cause of death, however, the pathology of
Enzymatic microreactor-on-a-chip: protein mapping using trypsin immobilized on porous polymer monoliths molded in channels of microfluidic devices.
Journal:Anal Chem
PubMed ID:12199578
'Enzymatic microreactors have been prepared in capillaries and on microfluidic chips by immobilizing trypsin on porous polymer monoliths consisting of 2-vinyl-4,4-dimethylazlactone, ethylene dimethacrylate, and acrylamide or 2-hydroxyethyl methacrylate. The azlactone functionalities react readily with amine and thiol groups of the enzyme to form stable covalent bonds. The optimized porous properties
Quenched BODIPY dye-labeled casein substrates for the assay of protease activity by direct fluorescence measurement.
Journal:Anal Biochem
PubMed ID:9299009
'We have prepared casein conjugates of two BODIPY dyes for use as fluorogenic protease substrates in homogeneous assays. Both conjugates are labeled to such an extent that the dyes are efficiently quenched in the protein, yielding virtually nonfluorescent substrate molecules. These fluorogenic substrates release highly fluorescent BODIPY dye-labeled peptides upon
Functional screening of intracellular proteins in single cells and in patterned cell arrays using electroporation.
Journal:Anal Chem
PubMed ID:12199607
'A tool for detection and characterization of intracellular enzyme-substrate and receptor-ligand interactions inside the cytoplasm of single targeted cells or small confined groups of cells is presented. Fluorogenic enzyme substrates and receptor ligands were rapidly delivered by electroosmosis and internalized by electroporation in cells using an electrolyte-filled capillary (EFC) biased