EnzChek™ 焦磷酸盐检测试剂盒
EnzChek™ 焦磷酸盐检测试剂盒
Invitrogen™

EnzChek™ 焦磷酸盐检测试剂盒

EnzChek™ 焦磷酸盐检测试剂盒提供了一种快速且高度灵敏的酶检测方法,可用于检测溶液中的游离焦磷酸盐。在该检测中,使用分光光度法检测形成的显色产物。查看我们完整的荧光微孔板检测产品线。•灵敏度限值为 1 µM 焦磷酸盐(∼0.2 µg/mL了解更多信息
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货号数量
E6645100 Assays
货号 E6645
价格(CNY)
6,660.00
Each
添加至购物车
数量:
100 Assays
价格(CNY)
6,660.00
Each
添加至购物车
EnzChek™ 焦磷酸盐检测试剂盒提供了一种快速且高度灵敏的酶检测方法,可用于检测溶液中的游离焦磷酸盐。在该检测中,使用分光光度法检测形成的显色产物。

查看我们完整的荧光微孔板检测产品线

•灵敏度限值为 1 µM 焦磷酸盐(∼0.2 µg/mL)
•可适应 6.5-8.5 的 pH 值范围

EnzChek™ 焦磷酸盐检测试剂盒提供了一种快速、便利且价格便宜的分光光度检测法,可用于测定一系列生物化学反应产生的无机焦磷酸盐,例如 DNA 和 RNA 聚合、由腺苷酸环化酶形成环 AMP 以及脂肪酸被酶激活后形成脂酰辅酶 A。

EnzChek™ 焦磷酸盐测定试剂盒是 EnzChek™ 磷酸盐测定试剂盒的改进产品(货号:E6646)。在存在无机磷酸盐的情况下,底物 2-氨基-6-巯基-7-甲基嘌呤核糖核苷 (MESG) 被嘌呤核苷磷酸化酶 (PNP) 转化为核糖 1-磷酸和 2-氨基-6-巯基-7-甲基嘌呤。MESG 的酶转化导致最大吸光度波长从 330 nm 变为 360 nm。EnzChek™ 焦磷酸盐检测试剂盒包括无机焦磷酸酶,它能够催化焦磷酸盐转化为两个当量的磷酸盐。然后,磷酸盐被 MESG/PNP 反应消耗,可通过在 360 nm 波长处的吸光度增加进行检测。通过将一分子焦磷酸盐扩增为两分子磷酸盐可获得更高的灵敏度。
仅供科研使用。不可用于诊断程序。
规格
检测方法比色法
染料类型MESG
产品规格
数量100 Assays
运输条件室温
颜色紫外线
适用于(应用)焦磷酸盐检测
适用于(设备)分光光度计
产品线EnzChek
产品类型焦磷酸盐检测
Unit SizeEach
内容与储存

包含无机焦磷酸酶、2-氨基-6-巯基-7-甲基嘌呤核糖苷 (MESG)、嘌呤核苷磷酸化酶 (PNP)、浓缩反应缓冲液、Na2P2O7 标准品以及用于检测和定量焦磷酸盐的详细实验方案。

常见问题解答 (FAQ)

What is the sensitivity limit of the EnzCheck Pyrophosphate Assay kit?

The sensitivity limit is 1 mM pyrophosphate (approximately 0.2 µg/mL).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (10)

引用和文献
Abstract
Presence of a plant-like proton-pumping pyrophosphatase in acidocalcisomes of Trypanosoma cruzi.
Authors:Scott DA, de Souza W, Benchimol M, Zhong L, Lu HG, Moreno SN, Docampo R
Journal:J Biol Chem
PubMed ID:9705361
'The vacuolar-type proton-translocating pyrophosphatase (V-H+-PPase) is an enzyme previously described in detail only in plants. This paper demonstrates its presence in the trypanosomatid Trypanosoma cruzi. Pyrophosphate promoted organellar acidification in permeabilized amastigotes, epimastigotes, and trypomastigotes of T. cruzi. This activity was stimulated by K+ ions and was inhibited by Na+ ... More
A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activity.
Authors:Lloyd AJ, Thomann HU, Ibba M, Söll D
Journal:Nucleic Acids Res
PubMed ID:7659511
'We describe a convenient, simple and novel continuous spectrophotometric method for the determination of aminoacyl-tRNA synthetase activity. The assay relies upon the measurement of inorganic pyrophosphate generated in the first step of the aminoacylation of a tRNA. Pyrophosphate release is coupled to inorganic pyrophosphatase, to generate phosphate, which in turn ... More
Thiamin biosynthesis in Escherichia coli. Identification of this thiocarboxylate as the immediate sulfur donor in the thiazole formation.
Authors:Taylor SV, Kelleher NL, Kinsland C, Chiu HJ, Costello CA, Backstrom AD, McLafferty FW, Begley TP
Journal:J Biol Chem
PubMed ID:9632726
ThiFSGH and ThiI are required for the biosynthesis of the thiazole moiety of thiamin in Escherichia coli. The overproduction, purification, and characterization of ThiFS and the identification of two of the early steps in the biosynthesis of the thiazole moiety of thiamin are described here. ThiS isolated from E. coli ... More
AcsD catalyzes enantioselective citrate desymmetrization in siderophore biosynthesis.
Authors:Schmelz S, Kadi N, McMahon SA, Song L, Oves-Costales D, Oke M, Liu H, Johnson KA, Carter LG, Botting CH, White MF, Challis GL, Naismith JH,
Journal:Nat Chem Biol
PubMed ID:19182782
Bacterial pathogens need to scavenge iron from their host for growth and proliferation during infection. They have evolved several strategies to do this, one being the biosynthesis and excretion of small, high-affinity iron chelators known as siderophores. The biosynthesis of siderophores is an important area of study, not only for ... More
A spectrophotometric method to measure enzymatic activity in reactions that generate inorganic pyrophosphate.
Authors:Upson RH, Haugland RP, Malekzadeh MN, Haugland RP
Journal:Anal Biochem
PubMed ID:8954523
This paper describes a spectrophotometric assay that can measure the inorganic pyrophosphate produced from various enzymatic reactions. This is a coupled assay in which the addition of inorganic pyrophosphatase initially cleaves the pyrophosphate into two molecules of phosphate. The phosphate is then detected by the conversion of 2-amino-6-mercapto-7-methylpurine ribonucleoside to ... More