BODIPY-alpha-casein, a pH-independent protein substrate for protease assays using fluorescence polarization.
AuthorsSchade SZ, Jolley ME, Sarauer BJ, Simonson LG
JournalAnal Biochem
PubMed ID8954519
'BODIPY-alpha-casein is a new fluorescent protein substrate designed for fluorescence polarization studies to measure proteolytic activity at any pH over the range from pH 2 to 11. Kinetic protease assays in real-time were performed in 1 to 5 min using an FPM-1 fluorescence polarization instrument. A purified enzyme or bacterial ... More
Measurement of proteases in human subgingival dental plaque by fluorescence polarization.
AuthorsGrys EL, Schade SZ, Cohen ME, Geivelis M, Robinson PJ, Simonson LG
JournalArch Oral Biol
PubMed ID11084150
'Fluorescence polarization (FP) was examined as a rapid quantitative method to assay the proteases in subgingival plaque. Protease activity was measured by a decrease in FP at 0.5-min intervals over 5 min, using BODIPY-alpha-casein, a protein substrate. To quantitate activity, the least absolute deviation (LAD) slope for each assay was ... More