Phalloidin-eosin followed by photo-oxidation: a novel method for localizing F-actin at the light and electron microscopic levels.
AuthorsCapani F, Deerinck TJ, Ellisman MH, Bushong E, Bobik M, Martone ME
JournalJ Histochem Cytochem
PubMed ID11668188
We describe a novel high-resolution method to detect F-actin at the light and electron microscopic levels through the use of the actin-binding protein phalloidin conjugated to the fluorophore eosin, followed by photo-oxidation of diaminobenzidine. This method possesses several key advantages over antibody-based labeling and structural methods. First, phalloidin binding to ... More
Filamentous actin is concentrated in specific subpopulations of neuronal and glial structures in rat central nervous system.
AuthorsCapani F, Ellisman MH, Martone ME
JournalBrain Res
PubMed ID11743966
This paper is the second in a series of studies on the light and electron microscopic distribution of filamentous actin (F-actin) in the rat central nervous system (CNS) using phalloidin tagged with the fluorophore eosin followed by fluorescence photooxidation. A previous report described the selective localization of high concentrations of ... More