Threonine phosphorylations induced by RX-871024 and insulin secretagogues in betaTC6-F7 cells.
AuthorsAn J, Zhao G, Churgay LM, Osborne JJ, Hale JE, Becker GW, Gold G, Stramm LE, Shi Y,
JournalAm J Physiol
PubMed ID10567013
'Treatment of the pancreatic beta-cell line betaTC6-F7 with an imidazoline compound, RX-871024, KCl, or tolbutamide resulted in increased threonine phosphorylation of a 220-kDa protein (p220) concurrent with enhanced insulin secretion, which can be partially antagonized by diazoxide, an ATP-sensitive potassium (K(ATP)) channel activator. Although phosphorylation of p220 was regulated by ... More
Role of thymosin beta4 in tumor metastasis and angiogenesis.
AuthorsCha HJ, Jeong MJ, Kleinman HK,
JournalJ Natl Cancer Inst
PubMed ID14625258
'BACKGROUND: Expression of the small peptide thymosin beta4 is associated with angiogenesis induction, accelerated wound healing, and the metastatic potential of tumor cells. However, little is known about the mechanism(s) by which thymosin beta4 promotes metastasis. METHODS: Northern blot analysis and immunohistochemistry were used to examine thymosin beta4 expression in ... More
Purification and characterization of a protein that permits early detection of lung cancer. Identification of heterogeneous nuclear ribonucleoprotein-A2/B1 as the antigen for monoclonal antibody 703D4.
AuthorsZhou J, Mulshine JL, Unsworth EJ, Scott FM, Avis IM, Vos MD, Treston AM,
JournalJ Biol Chem
PubMed ID8631886
'We have reported that a mouse monoclonal antibody 703D4, detects lung cancer 2 years earlier than routine chest x-ray or cytomorphology. We purified the 703D4 antigen to elucidate its role in early lung cancer biology, using Western blot detection after SDS-polyacrylamide gel electrophoresis. Purification steps included anion exchange chromatography, preparative ... More