Novex® 4-20% Tris-Glycine Express Kit 1.5 mm, 15 well - FAQs

Are there any suggestions on how to reduce high background when using the Colloidal Blue Staining Kit on Invitrogen Tris-Glycine gels?

It may be possible to reduce background by using the protocol given for NuPAGE Invitrogen Bis-Tris/Small Peptides.

This protocol incorporates an extra fix step to remove excess SDS, which can act as an anti-colloidal agent and lead to higher background.

The low pH of the staining solution will fix the gel, but not as fast as the pre-fix step specified in the NuPAGE protocol.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.