EC6042BOX - FAQs

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2 个常见问题解答

What is meant by the terms "Straightness" and "Curvature" on the Certificate of Analysis for a Invitrogen protein gel?

Gel straightness is defined as the straightness across all lanes of the gel, measured at the bottom, expressed relative to the total length of the gel. For example, a gel with straightness of 0.020 Rf is flat to within 2% of the length of the gel (1.6 mm) across. Band curvature is defined as the curvature of the bands in the outer lanes of the gel, expressed relative to the total length of the gel. For example, bands with curvature of 0.010 Rf are straight to within 1% of the length of the gel (0.8 mm).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedures are carried out for QC of Invitrogen gels?

The QC of our gels includes several processes:

1) Each gel is checked by eye for visible anomalies.

2) Under defined conditions, gels retained from each lot are tested as follows:

--When gels are run at a defined voltage, the resulting current and power of the electrophoresis are measured.

--Protein samples are electrophoresed on test gels to determine the gel run time and the protein band quality after electrophoresis. Bands are examined for: straightness within bands, curvature of bands across the gel ("smiling" or "frowning"), and reproducibility of the Rf values for protein molecular weight markers. According to these results, a Certificate of Analysis is created, which is available upon request.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.