Novex™ TBE 凝胶，4-12%，12 孔板

引用和文献 (15)

Invitrogen™

Novex™ TBE 凝胶，4-12%，12 孔板

Novex™ TBE 凝胶在分析限制性酶切和 PCR 产物时具有可能的较高分离度。DNA 片段可以清晰地分离出尖锐、紧密的条带。Novex™ TBE了解更多信息

货号	数量
EC62352BOX	1 box

货号 EC62352BOX

价格（CNY)

1,801.00

Ends: 31-Dec-2025

2,145.00

共减 344.00 (16%)

添加至购物车

1 box

请求批量或定制报价

价格（CNY)

1,801.00

Ends: 31-Dec-2025

2,145.00

共减 344.00 (16%)

添加至购物车

Novex™ TBE 凝胶在分析限制性酶切和 PCR 产物时具有可能的较高分离度。DNA 片段可以清晰地分离出尖锐、紧密的条带。Novex™ TBE 凝胶适合在 XCell SureLock™ Mini-Cell 小型电泳仪上运行。

配方
Novex™ TBE 凝胶使用高纯度 Tris base、硼酸、EDTA、丙烯酰胺、双丙烯酰胺、TEMED 和 APS 制作而成。

推荐缓冲液
为获得更佳性能，强烈推荐与 Novex™ TBE 电泳缓冲液和 Novex™ 高密度 TBE 上样缓冲液配合使用。Novex™ 高密度 TBE 上样缓冲液包含密度剂 Ficoll™（可产出比传统密度剂更尖锐、更直的条带）以及示踪染料溴酚蓝和二甲苯青。

仅供科研使用。不可用于诊断程序。

描述Novex TBE Gels, 4 to 12%, 12-well

产品类型TBE 凝胶

数量1 box

样品类型Double-stranded DNA (dsDNA)

运输条件湿冰

适用于（设备）XCell SureLock Mini-Cell

凝胶百分比4 至 12%

凝胶类型TBE 胶

分离范围35 to 400 bp

Unit SizeEach

内容与储存

• 货号是指一盒10块凝胶

在 4°C 下储存。

常见问题解答 (FAQ)

我能否对TBE凝胶染色？如何染色？

是的，您可以使用溴化乙锭、SYBR Green I、SYBR Green II和SilverXpress银染试剂盒对TBE凝胶染色。采用溴化乙锭染色时，将胶侵泡在使用超纯水配制的2 µg/mL溴化乙锭溶液中20分钟。染色后使用超纯水连续冲洗3次，每次10分钟，除去未结合染料。在紫外灯下观察条带。

TBE凝胶上可见的最小片段长度是多少？

在Invitrogen 10% TBE凝胶上，51 bp的marker条带清晰可见。在Invitrogen 20% TBE凝胶上，18和12 bp的marker条带清晰可见.

TBE凝胶中的EDTA浓度是多少？甘油呢？

我们的TBE凝胶中EDTA浓度是0.06% (质量/体积)。20% TBE凝胶包含4%甘油以获得最大分辨率。所有其它TBE凝胶均仅在凝胶底部9%的范围内含有0.8%甘油，在凝胶的其它部分没有甘油。

我可以对TBE凝胶或TBE-尿素凝胶染色吗？如何染色？

可以，对于EB染色，将凝胶在超纯水配制的2 µg/mL EB溶液中浸泡20分钟。接下来使用超纯水进行3次洗涤脱色，每次10分钟。在紫外光下观察条带。

TBE凝胶的电泳条件（电压，电流，电泳时间等）是什么？

电压：200V恒定电压*
起始电流大约为：10–18 mA/凝胶
结束时电流大约为：4–6 mA/凝胶
电泳时间：大约30–90分钟，取决于凝胶的浓度。当溴酚蓝（深色）示踪染料到达凝胶的底部时电泳结束。
*可以使用最高250 V的电压以减少电泳时间。

View all Novex™ TBE 凝胶，4-12%，12 孔板 FAQs

引用和文献 (15)

引用和文献

Abstract

Prevalence and correlates of GB virus C infection in HIV-infected and HIV-uninfected pregnant women in Bangkok, Thailand.

Authors:Bhanich Supapol W, Remis RS, Raboud J, Millson M, Tappero J, Kaul R, Kulkarni P, McConnell MS, Mock PA, McNicholl JM, Roongpisuthipong A, Chotpitayasunondh T, Shaffer N, Butera S

Journal:J Med Virol

PubMed ID:21108337

'GB virus C (GBV-C) is an apathogenic virus that has been shown to inhibit HIV replication. This study examined the prevalence and correlates of GBV-C infection and clearance in three cohorts of pregnant women in Thailand. The study population consisted of 1,719 (1,387 HIV-infected and 332 HIV-uninfected) women from three ... More

An in vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides.

Authors:Datta K, Purkayastha S, Neumann RD, Winters TA

Journal:Methods Mol Biol

PubMed ID:22941624

'DNA double-strand breaks (DSBs) are caused by endogenous cellular processes such as oxidative metabolism, or by exogenous events like exposure to ionizing radiation or other genotoxic agents. Repair of these DSBs is essential for the maintenance of cellular genomic integrity. In human cells, and cells of other higher eukaryotes, DSBs ... More

Smooth muscle phenotypic diversity is mediated through alterations in myocardin gene splicing.

Authors:Ilagan RM, Genheimer CW, Quinlan SF, Guthrie KI, Sangha N, Ramachandrannair S, Kelley RW, Presnell SC, Basu J, Ludlow JW

Journal:J Cell Physiol

PubMed ID:21792927

'Myocardin (MYOCD) is a smooth and cardiac muscle-specific transcriptional coactivator that is required for the proper expression of contraction-related genes. Through its function to transactivate effector genes, MYOCD plays an essential role in mediating the switch between contractile and non-contractile phenotypes, particularly in smooth muscle cells (SMC). There are at ... More

Genome wide full-length transcript analysis using 5' and 3' paired-end-tag next generation sequencing (RNA-PET).

Authors:Ruan X, Ruan Y

Journal:Methods Mol Biol

PubMed ID:22113299

'RNA-PET is a paired end tag (PET) sequencing method for full-length mRNA transcripts analysis using the next generation sequencer platforms such as Illumina GA and SOLiD. Unlike RNA-Seq method that sequences randomly sheared shotgun RNA short fragments, RNA-PET captures and sequences the 5'' and 3'' end tags of full-length cDNA ... More

Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition.

Authors:Adey A, Morrison HG, Asan Xun X, Kitzman JO, Turner EH, Stackhouse B, MacKenzie AP, Caruccio NC, Zhang X, Shendure J

Journal:Genome Biol

PubMed ID:21143862

We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its ... More

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