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4-12% TBE Gels 1.0 mm, 10 well -"DISCONTINUED" - FAQs

查看更多产品信息 4-12% TBE Gels 1.0 mm, 10 well -"DISCONTINUED" - FAQs (EC6235BOX)

14 个常见问题解答

我能否对TBE凝胶染色？如何染色？

是的，您可以使用溴化乙锭、SYBR Green I、SYBR Green II和SilverXpress银染试剂盒对TBE凝胶染色。采用溴化乙锭染色时，将胶侵泡在使用超纯水配制的2 µg/mL溴化乙锭溶液中20分钟。染色后使用超纯水连续冲洗3次，每次10分钟，除去未结合染料。在紫外灯下观察条带。

TBE凝胶上可见的最小片段长度是多少？

在Invitrogen 10% TBE凝胶上，51 bp的marker条带清晰可见。在Invitrogen 20% TBE凝胶上，18和12 bp的marker条带清晰可见.

TBE凝胶中的EDTA浓度是多少？甘油呢？

我们的TBE凝胶中EDTA浓度是0.06% (质量/体积)。20% TBE凝胶包含4%甘油以获得最大分辨率。所有其它TBE凝胶均仅在凝胶底部9%的范围内含有0.8%甘油，在凝胶的其它部分没有甘油。

我可以对TBE凝胶或TBE-尿素凝胶染色吗？如何染色？

可以，对于EB染色，将凝胶在超纯水配制的2 µg/mL EB溶液中浸泡20分钟。接下来使用超纯水进行3次洗涤脱色，每次10分钟。在紫外光下观察条带。

TBE凝胶的电泳条件（电压，电流，电泳时间等）是什么？

电压：200V恒定电压*
起始电流大约为：10–18 mA/凝胶
结束时电流大约为：4–6 mA/凝胶
电泳时间：大约30–90分钟，取决于凝胶的浓度。当溴酚蓝（深色）示踪染料到达凝胶的底部时电泳结束。
*可以使用最高250 V的电压以减少电泳时间。

TBE凝胶的技术指标是什么，特别是凝胶和胶盒的尺寸如何？

请参见下列凝胶技术指标：

凝胶基质：丙烯酰胺/双丙烯酰胺
凝胶厚度：1.0 mm和1.5 mm
凝胶尺寸：8 cm x 8 cm (高x 宽)
胶盒尺寸：10 cm x 10 cm(高x 宽)

TBE凝胶的保质期和保存条件是什么？

TBE凝胶的保质期是8周，应保存于4°C。

Can I stain my TBE gel? How?

Yes, you can stain your TBE gels with ethidium bromide, SYBR Green I, SYBR Green II, and the SilverXpress Silver Staining Kit. For ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light.

What are the smallest fragments that can be visualized on the TBE gels?

On the Invitrogen 10% TBE gels, a 51 bp marker can be clearly seen. On the Invitrogen 20% TBE gel, the 18 and 12 bp markers can be clearly seen.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the concentration of EDTA in the TBE gels? How about glycerol?

The EDTA concentration in our TBE gels is 0.06% (w/v). The 20% TBE gels contain 4% glycerol for maximal resolution. All other TBE gels contain 0.8% glycerol in a layer that represents the bottom 9% of the gel. There is no glycerol in the rest of the gel.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I stain my TBE gel or my TBE-urea gel? How?

Yes, for ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What should the running conditions be for the TBE gels (voltage, current, run time, etc.)?

Voltage: 200 V constant*
Approximate current at start: 10-18 mA/gel
Approximate current at end: 4-6 mA/gel
Run time: Approximately 30-90 minutes, dependent on gel percentage. The run is complete when the bromophenol blue (darker) tracking dye reaches the bottom of the gel.

* Voltages up to 250 V may be used to reduce run time.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What are the TBE gel specifications, specifically the gel size and cassette size?

Please see the gel specifications below:

Gel matrix: Acrylamide/bis-acrylamide
Gel thickness: 1.0 mm and 1.5 mm
Gel size: 8 cm x 8 cm (height x width)
Cassette size: 10 cm x 10 cm (height x width)

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What are the shelf life and storage conditions for my TBE gels?

The shelf life is 8 weeks for the gels, which should be stored at 4°C.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.