Novex™ TBE Gels, 6%
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引用和文献 (15)
Invitrogen™

Novex™ TBE Gels, 6%

Novex™ TBE Gels 6% are designed to run on the XCell SureLock™ Mini-Cell and provide high-resolution analysis of restriction digests and PCR products.
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货号孔
EC62652BOX12孔
EC6261BOX1孔
EC6265BOX10孔
EC62655BOX15孔
货号 EC62652BOX
价格（CNY)
2,137.00
Each
添加至购物车
孔:
12孔
请求批量或定制报价
价格（CNY)
2,137.00
Each
添加至购物车
Novex™ TBE Gels 6% provide high-resolution analysis of restriction digests and PCR products. Designed to run on the XCell SureLock™ Mini-Cell, the polyacrylamide gels give sharp, clearly resolved, intense bands, and provide separations of double-strand DNA fragments from 65–250 bp.

  • DNA 片段可以被清晰地分离出明显紧密的条带
  • 适合在 XCell SureLock™ Mini-Cell 小型电泳仪上运行
  • 使用高纯度 Tris 碱、硼酸、EDTA、丙烯酰胺、双丙烯酰胺、TEMED 和 APS 制作而成

琼脂糖凝胶电泳、DNA 和 RNA 纯化及分析、核酸凝胶电泳和印迹杂交

For Research Use Only. Not for use in diagnostic procedures.
规格
描述Novex TBE Gels, 6%, 12-well
产品类型TBE 凝胶
数量10 块凝胶/盒
样品类型Double-stranded DNA (dsDNA)
运输条件湿冰
适用于（设备）XCell SureLock Mini-Cell
凝胶百分比6%
凝胶类型TBE 胶
分离范围65 to 250 bp
孔12孔
Unit SizeEach
内容与储存
• 货号是指一盒10块凝胶

在 4°C 下储存。

常见问题解答 (FAQ)

我能否对TBE凝胶染色？如何染色？

是的，您可以使用溴化乙锭、SYBR Green I、SYBR Green II和SilverXpress银染试剂盒对TBE凝胶染色。采用溴化乙锭染色时，将胶侵泡在使用超纯水配制的2 µg/mL溴化乙锭溶液中20分钟。染色后使用超纯水连续冲洗3次，每次10分钟，除去未结合染料。在紫外灯下观察条带。

Invitrogen 6% TBE凝胶和Invitrogen 6% DNA阻滞凝胶的成分有什么区别？

Invitrogen 6% DNA阻滞凝胶包含0.5X TBE。两种凝胶均能用于凝胶阻滞实验；但是，Invitrogen 6% TBE凝胶内的1X TBE有更高的离子环境，可能会影响DNA-蛋白相互作用。Invitrogen 6% DNA阻滞凝胶内的0.5X TBE通常效果更好，因为它在电泳中有较高的片段分离效果而且其离子强度较低，可以促进DNA-蛋白相互作用。

TBE凝胶上可见的最小片段长度是多少？

在Invitrogen 10% TBE凝胶上，51 bp的marker条带清晰可见。在Invitrogen 20% TBE凝胶上，18和12 bp的marker条带清晰可见.

TBE凝胶中的EDTA浓度是多少？甘油呢？

我们的TBE凝胶中EDTA浓度是0.06% (质量/体积)。20% TBE凝胶包含4%甘油以获得最大分辨率。所有其它TBE凝胶均仅在凝胶底部9%的范围内含有0.8%甘油，在凝胶的其它部分没有甘油。

我可以对TBE凝胶或TBE-尿素凝胶染色吗？如何染色？

可以，对于EB染色，将凝胶在超纯水配制的2 µg/mL EB溶液中浸泡20分钟。接下来使用超纯水进行3次洗涤脱色，每次10分钟。在紫外光下观察条带。

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引用和文献 (15)

引用和文献
Abstract
Prevalence and correlates of GB virus C infection in HIV-infected and HIV-uninfected pregnant women in Bangkok, Thailand.
Authors:Bhanich Supapol W, Remis RS, Raboud J, Millson M, Tappero J, Kaul R, Kulkarni P, McConnell MS, Mock PA, McNicholl JM, Roongpisuthipong A, Chotpitayasunondh T, Shaffer N, Butera S
Journal:J Med Virol
PubMed ID:21108337
'GB virus C (GBV-C) is an apathogenic virus that has been shown to inhibit HIV replication. This study examined the prevalence and correlates of GBV-C infection and clearance in three cohorts of pregnant women in Thailand. The study population consisted of 1,719 (1,387 HIV-infected and 332 HIV-uninfected) women from three ... More
An in vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides.
Authors:Datta K, Purkayastha S, Neumann RD, Winters TA
Journal:Methods Mol Biol
PubMed ID:22941624
'DNA double-strand breaks (DSBs) are caused by endogenous cellular processes such as oxidative metabolism, or by exogenous events like exposure to ionizing radiation or other genotoxic agents. Repair of these DSBs is essential for the maintenance of cellular genomic integrity. In human cells, and cells of other higher eukaryotes, DSBs ... More
Smooth muscle phenotypic diversity is mediated through alterations in myocardin gene splicing.
Authors:Ilagan RM, Genheimer CW, Quinlan SF, Guthrie KI, Sangha N, Ramachandrannair S, Kelley RW, Presnell SC, Basu J, Ludlow JW
Journal:J Cell Physiol
PubMed ID:21792927
'Myocardin (MYOCD) is a smooth and cardiac muscle-specific transcriptional coactivator that is required for the proper expression of contraction-related genes. Through its function to transactivate effector genes, MYOCD plays an essential role in mediating the switch between contractile and non-contractile phenotypes, particularly in smooth muscle cells (SMC). There are at ... More
Genome wide full-length transcript analysis using 5' and 3' paired-end-tag next generation sequencing (RNA-PET).
Authors:Ruan X, Ruan Y
Journal:Methods Mol Biol
PubMed ID:22113299
'RNA-PET is a paired end tag (PET) sequencing method for full-length mRNA transcripts analysis using the next generation sequencer platforms such as Illumina GA and SOLiD. Unlike RNA-Seq method that sequences randomly sheared shotgun RNA short fragments, RNA-PET captures and sequences the 5'' and 3'' end tags of full-length cDNA ... More
Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition.
Authors:Adey A, Morrison HG, Asan Xun X, Kitzman JO, Turner EH, Stackhouse B, MacKenzie AP, Caruccio NC, Zhang X, Shendure J
Journal:Genome Biol
PubMed ID:21143862
We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its ... More
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