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Novex™ 4-16% Zymogram (Blue Casein) Protein Gels, 1.0 mm, 10-well - FAQs

View additional product information for Novex™ 4-16% Zymogram (Blue Casein) Protein Gels, 1.0 mm, 10-well - FAQs (EC6415BOX)

5 product FAQs found

Does the Colloidal Blue Staining kit work with Invitrogen Zymogram gels?

Yes, the Colloidal Blue Staining kit works very well with Invitrogen Zymogram gels.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the Brij 35 detergent concentration in the 1X Zymogram Developing buffer?

The Brij 35 detergent concentration for Zymogram Developing buffer should be 0.02% (w/v).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The concentration of casein in 4-16% Zymogram Blue-Casein gels is higher (0.1%) than that in the 12% Zymogram Casein gels (0.05%). Why is the sensitivity of 4-16% Zymogram Blue-Casein gels lower than that of the 12% Zymogram Casein gels?

The casein in the 4-16% Zymogram Blue-Casein gels is covalently bound to a dye. Some enzymes have a harder time digesting the dye-bound casein compared to the unmodified casein and also it takes more activity to clear the higher quantity of casein. Please note, our Zymogram Casein gels have been discontinued. However, we still offer Zymogram Gelatin Plus Gels (Cat. Nos. ZY00100BOX, ZY00102BOX, ZY00105BOX).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

In the literature, most of the Zymogram Casein gels contain 0.1% or 0.2% casein. Why do you use 0.05% casein in your 12% Zymogram Casein gels?

We decided to go with 0.05% casein because it gives higher sensitivity. 0.1% casein and 0.2% casein provide higher contrast of the bands but lower sensitivity because the enzyme has to digest more substrate in order to form the clear band. Please note, our Zymogram Casein gels have been discontinued. However, we still offer Zymogram Gelatin Plus Gels (Cat. Nos. ZY00100BOX, ZY00102BOX, ZY00105BOX).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What kind of casein is present in Invitrogen 4-16% Zymogram Blue-Casein gels?

Invitrogen 4-16% Zymogram Blue-Casein gels are 4-16% Tris-Glycine gels with 0.1% casein that is covalently linked to a proprietary dye, as the substrate. The casein is ~26 kDa in size. The type of casein used is proprietary. The presence of the dye makes visualization easy without having to stain the gel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.