Novex™ TBE-Urea Gels, 6%
Novex™ TBE-Urea Gels, 6%
Invitrogen™

Novex™ TBE-Urea Gels, 6%

变性聚丙烯酰胺凝胶,可将单链 DNA 寡核苷酸或 RNA 分解成明显、清晰的条带
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货号
EC6865BOX10孔
EC68652BOX12孔
EC68655BOX15孔
货号 EC6865BOX
价格(CNY)
2,145.00
Each
添加至购物车
孔:
10孔
请求批量或定制报价
价格(CNY)
2,145.00
Each
添加至购物车
Novex™ TBE-Urea Gels are denaturing polyacrylamide gels that resolve single-stranded DNA oligos or RNA into sharp, distinct bands. These 6% gels are optimized for the analysis and purification of products ranging from 25–110 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and northern blot analysis.

Novex TBE-Urea Gels can be stained by silver staining, ethidium bromide, and SYBR™ Green staining techniques after electrophoresis. The compact gels are available in 6%, 10%, or 15% formats and are designed to run on the XCell SureLock™ Mini-Cell.

Advantages of TBE-Urea Gels:
• Contains 7M urea for maximum denaturation
• Excellent resolution for fast size and purity confirmations of DNA or RNA oligos
• High resolution of short single-strand oligonucleotides
• Requires ∼10% sample concentration and volume of large or agarose gels
• Accurate and reproducible results

Formulation
Novex TBE-Urea gels are made with high-purity reagents, including Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, APS, and 7M urea. Strict quality control ensures that gels and buffers are DNase- and RNase-free.

Recommended Buffers
For optimum performance, Novex™ TBE-Urea sample buffers and Novex™ TBE Running Buffer are strongly recommended for use with these gels. For analytical applications, Novex TBE-Urea Sample Buffer is recommended contains urea, the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, and the tracking dyes Bromophenol Blue and Xylene Cyanol.

For Research Use Only. Not for use in diagnostic procedures.
规格
描述Novex TBE-Urea Gels, 6%, 10-well
产品类型TBE 凝胶
数量10 块凝胶/盒
样品类型Single-stranded DNA Oligos/RNA
运输条件湿冰
适用于(设备)XCell SureLock Mini-Cell
凝胶百分比6%
凝胶类型TBE-Urea
分离范围20 至 800 bp
10孔
Unit SizeEach
内容与储存
• 货号是指一盒10块凝胶
• Novex™ TBE 尿素凝胶的有效期为4至8周,具体取决于凝胶类型

在 4°C 下储存。

常见问题解答 (FAQ)

Invitrogen TBE-尿素系统可以使用含有福尔马林的上样缓冲液吗?

该系统可以使用多种上样缓冲液配方;但是,我们发现根据上样缓冲液的不同,电泳条带的形态会有很大的不同。在评估了尿素、福尔马林以及各种缓冲液系统之后,我们发现使用尿素、Ficoll和TBE缓冲溶液能获得最清晰、最整齐的条带。使用含福尔马林的上样缓冲液将得到模糊、不清晰的条带。

我可以对TBE凝胶或TBE-尿素凝胶染色吗?如何染色?

可以,对于EB染色,将凝胶在超纯水配制的2 µg/mL EB溶液中浸泡20分钟。接下来使用超纯水进行3次洗涤脱色,每次10分钟。在紫外光下观察条带。

Can I use a sample buffer containing formamide with the Invitrogen TBE-urea system?

There are many sample buffer formulations used; however, we have found a distinct difference in the band appearance depending on the sample buffer composition. After evaluating urea, formamide, and various buffer systems, we found that the sharpest, flattest bands were obtained with a urea, Ficoll, and TBE buffer solution. Sample buffers made with formamide provided fuzzy, indistinct bands.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I stain my TBE gel or my TBE-urea gel? How?

Yes, for ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

I am planning to use a TBE polyacrylamide gel for electrophoresis of my nucleic acid fragment. How do I know if the size of my fragment is suitable for a particular gel you offer?

To help you choose the TBE polyacrylamide gel that will provide optimal separation results for your nucleic acid fragment, we have designed gel migration charts for each gel type offered. Please check the TBE gel migration charts on page 74 of the Novex Pre-Cast Gel Electrophoresis Guide (https://tools.thermofisher.com/content/sfs/manuals/electrophoresisguide_man.pdf).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.