'An attenuated strain of Salmonella typhimurium was used as a vehicle for oral gene therapy against murine tumor. Eukaryotic expression vectors containing genes of human interleukin-12 (hIL-12), human granulocyte/macrophage colony-stimulating factor (hGM-CSF), mouse (m)IL-12, mGM-CSF and green fluorescent protein (GFP) were used to transform attenuated Salmonella (SL3261), and such transformants ... More
PK1/EG-VEGF induces monocyte differentiation and activation.
AuthorsDorsch M, Qiu Y, Soler D, Frank N, Duong T, Goodearl A, O'Neil S, Lora J, Fraser CC
JournalJ Leukoc Biol
PubMed ID15908459
'Macrophages exist as sentinels in innate immune response and react by expressing proinflammatory cytokines and up-regulating antigen-presenting and costimulatory molecules. We report a novel function for prokineticin-1 (PK1)/endocrine gland-derived vascular endothelial growth factor. Screening of murine tissue sections and cells for specific binding site leads to the identification of macrophages ... More
Cord blood interleukin-10 levels are increased in preterm newborns.
AuthorsBlanco-Quirós A, Arranz E, Solis G, Villar A, Ramos A, Coto D
JournalEur J Pediatr
PubMed ID10867846
'Cell-mediated immunosuppression due to interleukin (IL)-10 may contribute to normal pregnancy. By contrast, delivery is associated with a predominance of T-helper-1 (Th1) cytokines (IL-12, interferon-gamma) and might be regarded as a graft rejection process. The aim of the study was to assess IL-10 and IL-12 levels in cord blood samples ... More
In vitro immunoregulatory effects of antidepressants in healthy volunteers.
AuthorsSzuster-Ciesielska A, Tustanowska-Stachura A, Slotwinska M, Marmurowska-Michalowska H, Kandefer-Szerszen M
JournalPol J Pharmacol
PubMed ID14506314
'Major depression is accompanied by an activation of the inflammatory response system (IRS) and antidepressants may have immunoregulatory activities. This study was carried out to compare the effect of imipramine, mianserin and lithium on the in vitro production of Th1-like cytokines, such as IL-2, IFN-gamma, lymphotoxin and Th2-like cytokines such ... More
Tolerance is dependent on complement C3 fragment iC3b binding to antigen-presenting cells.
AuthorsSohn JH, Bora PS, Suk HJ, Molina H, Kaplan HJ, Bora NS,
JournalNat Med
PubMed ID12514742
'Systemic tolerance can be induced by the introduction of antigen into an immune-privileged site. Here we investigated the role of complement in the induction of tolerance after intraocular injection. We found that the development of antigen-specific tolerance is dependent on a complement activation product. The ligation of the complement C3 ... More
Apoptosis of blood mononuclear cells in alcoholic liver cirrhosis. The influence of in vitro ethanol treatment and zinc supplementation.
AuthorsSzuster-Ciesielska A, Daniluk J, Bojarska-Junak A
JournalToxicology
PubMed ID15964121
'Ethanol consumption induces apoptosis in a variety of tissues, among others in liver and lymphoid tissue. Zinc has been shown to influence apoptosis of blood mononuclear cells by inhibiting the mitochondrial pathway of cell death. The aim of this study was to examine the influence of zinc on spontaneous and ... More
Combination of interleukin 12 and interferon alpha gene therapy induces a synergistic antitumor response against colon and renal cell carcinoma.
AuthorsMendiratta SK, Quezada A, Matar M, Thull NM, Bishop JS, Nordstrom JL, Pericle F
JournalHum Gene Ther
PubMed ID10986558
'The antitumor effect and mechanism of action of IL-12 gene therapy combined with IFN-alpha gene therapy were investigated in tumor-bearing mice using renal and colon carcinoma models, Renca and CT26, respectively. Tumors were treated with murine IL-12 plasmid alone or in combination with IFN-alpha plasmid formulated with a polymeric interactive ... More
Dendritic cells from chronic hepatitis B patients can induce HBV antigen-specific T cell responses.
AuthorsLi RB, Chen HS, Xie Y, Fei R, Cong X, Jiang D, Wang SX, Wei L, Wang Y
JournalWorld J Gastroenterol
PubMed ID15162529
'To determine whether dendritic cells (DCs) from chronic hepatitis B patients could induce HBV antigen-specific T cell responses or not.'
Correlation of serum and ascitic IL-12 levels with second-look laparotomy results and disease progression in advanced epithelial ovarian cancer patients.
AuthorsBozkurt N, Yuce K, Basaran M, Gariboglu S, Kose F, Ayhan A
JournalInt J Gynecol Cancer
PubMed ID16445615
'Forty-two consecutive patients with advanced epithelial ovarian cancer who underwent primary surgical treatment were evaluated. The control group comprised 21 patients who had undergone surgery associated with benign pathologies. Forty-one patients had stage III disease except one who had stage IV. Optimal debulking (<1 cm) was performed in all the ... More
High efficiency transduction of dendritic cells by adenoviral vectors targeted to DC-SIGN.
AuthorsKorokhov N, de Gruijl TD, Aldrich WA, Triozzi PL, Banerjee PT, Gillies SD, Curiel TJ, Douglas JT, Scheper RJ, Curiel DT
JournalCancer Biol Ther
PubMed ID15753654
'Dendritic cells (DCs) are a central element in the development of antigen-specific immune responses. The lack of a specific and efficient technique for the in vivo delivery of antigens to DCs remains a major obstacle limiting a vaccine''s ability to induce an effective immune response. The efficacy of adenoviral (Ad) ... More
Circulating interleukin-18 and osteopontin are useful to evaluate disease activity in patients with tuberculosis.
AuthorsInomata S, Shijubo N, Kon S, Maeda M, Yamada G, Sato N, Abe S, Uede T
JournalCytokine
PubMed ID15863395
T helper type 1 (Th1) responses have been implicated in the protective immunity, pathophysiology and development of tuberculosis. However, it is still unclear which molecule(s) reflect disease activity in patients with tuberculosis. ... More
Intravesical instillation therapy with bacillus Calmette-Guérin for superficial bladder cancer: study of the mechanism of bacillus Calmette-Guérin immunotherapy.
AuthorsShintani Y, Sawada Y, Inagaki T, Kohjimoto Y, Uekado Y, Shinka T
JournalInt J Urol
PubMed ID17302571
In order to clarify the initial step of the mechanism by which bacillus Calmette-Guérin (BCG) exhibits antitumor activity via the immune response induced in the bladder submucosa after intravesical BCG therapy for human bladder cancer, various cytokines secreted in the urine after BCG instillation were measured. ... More
IL-8, IL-12 and IL-10 cytokines generation by neutrophils, fibroblasts and neutrophils- fibroblasts interaction in psoriasis.
AuthorsGlowacka E, Lewkowicz P, Rotsztejn H, Zalewska A
JournalAdv Med Sci
PubMed ID20934961
Psoriasis is a common skin disease affecting about 1-3% of the world population. Many types of cells, including lymphocytes, dendritics APCs (antigen presenting cells), NKT (natural killer T) cells, neutrophils, keratinocytes and fibroblasts are involved in the pathogenesis of psoriasis.The aim of our study was to assess in psoriatic patients ... More
Thalidomide and a thalidomide analogue drug costimulate virus-specific CD8+ T cells in vitro.
AuthorsHaslett PA, Hanekom WA, Muller G, Kaplan G
JournalJ Infect Dis
PubMed ID12660941
CD8(+) T cell immunity is critical for protection from viral disease, such as that caused by the human immunodeficiency virus (HIV) or cytomegalovirus (CMV). It is therefore important to identify therapies that can boost antiviral immunity. The recent finding that thalidomide acts as a T cell costimulator suggested that this ... More
The immunomodulatory effects of thalidomide on human immunodeficiency virus-infected children.
AuthorsHanekom WA, Hughes J, Haslett PA, Apolles P, Ganiso V, Allin R, Goddard E, Hussey GD, Kaplan G
JournalJ Infect Dis
PubMed ID11598844
The safety and immune effects of low-dose thalidomide treatment (3 mg/kg/day for 28 days) were evaluated in a study involving 8 South African human immunodeficiency virus (HIV)-infected children. The children were 7-69 months old and in disease stages A1-C3. Thalidomide therapy did not affect virus load, even though none of ... More
IL-10 levels in Dengue patients: some findings from the exceptional epidemiological conditions in Cuba.
AuthorsPérez AB, García G, Sierra B, Alvarez M, Vázquez S, Cabrera MV, Rodríguez R, Rosario D, Martínez E, Denny T, Guzmán MG
JournalJ Med Virol
PubMed ID15122797
The pathogenesis associated with Dengue haemorrhagic fever, has yet to be fully elucidated, with no definitive in vivo evidence. The exceptional epidemiological circumstances in Cuba allow the evaluation of different mediators in a well-defined situation. In the present study, we describe the determination of levels of IL-12, IL-10 and RANTES ... More
Influence of leptin levels and body weight in survival of children with sepsis.
AuthorsBlanco-Quirós A, Casado-Flores J, Arranz E, Garrote JA, Asensio J, Pérez A
JournalActa Paediatr
PubMed ID12162591
High levels of serum leptin (LPT) were reported in adult patients with sepsis and a protective role was suggested. LPT was determined in sera from 55 children with severe sepsis at admission (0 h), 6, 24 and 48 h. LPT levels were higher at 0 h than at 24 h ... More
Induction of apoptosis and release of interleukin-1 beta by cell wall-associated 19-kDa lipoprotein during the course of mycobacterial infection.
AuthorsCiaramella A, Cavone A, Santucci MB, Garg SK, Sanarico N, Bocchino M, Galati D, Martino A, Auricchio G, D'Orazio M, Stewart GR, Neyrolles O, Young DB, Colizzi V, Fraziano M
JournalJ Infect Dis
PubMed ID15319868
Mycobacterium tuberculosis induces apoptosis in human monocyte-derived macrophages (MDMs) during the early stages of infection. We investigated the proapoptotic role of cell wall-associated mycobacterial 19-kDa lipoprotein and the possible association between 19-kDa lipoprotein signaling and production of proinflammatory cytokines. Purified mycobacterial 19-kDa lipoprotein, 19-kDa lipoprotein-expressing M. smegmatis (M. smegmatis 19+), ... More
Apolipoprotein A-I induces IL-10 and PGE2 production in human monocytes and inhibits dendritic cell differentiation and maturation.
AuthorsKim KD, Lim HY, Lee HG, Yoon DY, Choe YK, Choi I, Paik SG, Kim YS, Yang Y, Lim JS
JournalBiochem Biophys Res Commun
PubMed ID16259956
Apolipoprotein A-I (apoA-I), the major protein component of serum high-density lipoprotein, exhibits anti-inflammatory activity in atherosclerosis. In this study, we demonstrate that apoA-I inhibits DC differentiation and maturation. DC differentiated from monocytes in the presence of apoA-I showed a decreased expression of surface molecules such as CD1a, CD80, CD86, and ... More
Prostaglandin E2 is a negative regulator on human plasmacytoid dendritic cells.
AuthorsSon Y, Ito T, Ozaki Y, Tanijiri T, Yokoi T, Nakamura K, Takebayashi M, Amakawa R, Fukuhara S
JournalImmunology
PubMed ID16762028
Prostaglandin E2 (PGE2), a major lipid derived from the metabolism of arachidonic acid, is an environmentally bioactive substance produced by inflammatory processes and acts as a cAMP up-regulator that plays an important role in immune responses. It has been reported that PGE2 has the ability to inhibit the production of ... More
IFN-alpha-conditioned dendritic cells are highly efficient in inducing cross-priming CD8(+) T cells against exogenous viral antigens.
AuthorsLapenta C, Santini SM, Spada M, Donati S, Urbani F, Accapezzato D, Franceschini D, Andreotti M, Barnaba V, Belardelli F
JournalEur J Immunol
PubMed ID16856207
Dendritic cells (DC) generated after a short-term exposure of monocytes to IFN-alpha and GM-CSF (IFN-DC) are highly effective in inducing cross-priming of CD8(+ )T cells against viral antigens. We have investigated the mechanisms responsible for the special attitude of these DC and compared their activity with that of reference DC. ... More
Monocyte-derived CD1a+ dendritic cells generated in two different culture systems: immunophenotypic and functional comparison.
AuthorsXia CQ, Kao KJ
JournalScand J Immunol
PubMed ID12662295
Previous studies demonstrated that CD1a+ dendritic cells (DCs) could not be prepared ex vivo without using fetal calf serum (FCS). Recently, we developed a method of using heparin to induce differentiation of human monocytes into CD1a+ DCs without using FCS. In order to determine the potential clinical applicability of heparin-induced ... More
Effect of PSK on the maturation of dendritic cells derived from human peripheral blood monocytes.
AuthorsKanazawa M, Mori Y, Yoshihara K, Iwadate M, Suzuki S, Endoh Y, Ohki S, Takita K, Sekikawa K, Takenoshita S
JournalImmunol Lett
PubMed ID15019294
Dendritic cells (DCs) are powerful antigen-presenting cells (APCs) that have attracted attention in recent years from the viewpoint of DC vaccine therapy against cancer. However, the existence of a strongly immunosuppressed state in cancer-bearing individuals inhibits DC maturation, which is one of the problems facing anti-cancer DC vaccine therapy. Protein-bound ... More
Monocytes promote natural killer cell interferon gamma production in response to the endogenous danger signal HMGB1.
AuthorsDeMarco RA, Fink MP, Lotze MT
JournalMol Immunol
PubMed ID15607795
Substantial attention has been paid to the role of the toll-like receptor (TLR) ligands of late and their role in regulating the innate immune response. They serve as exogenous danger signals important in informing and driving the distal adaptive immune response to pathogens. Less clear has been the role of ... More
Activation of selective transcription factors and cytokines by water-soluble extract from Lentinus lepideus.
AuthorsJin M, Jung HJ, Choi JJ, Jeon H, Oh JH, Kim B, Shin SS, Lee JK, Yoon K, Kim S
JournalExp Biol Med (Maywood)
PubMed ID12773708
We isolated a water-soluble extract, PG101, from cultured mycelia of Lentinus lepideus. Treatment of human peripheral blood mononuclear cells (PBMCs) with PG101 increased levels of TNF-alpha, IL-1beta, IL-10, and IL-12 by 100- to 1000-fold, whereas GM-CSF and IL-18 were activated by an order of magnitude. On the contrary, IFN-gamma and ... More
Phase I study of the intratumoral administration of recombinant canarypox viruses expressing B7.1 and interleukin 12 in patients with metastatic melanoma.
AuthorsTriozzi PL, Allen KO, Carlisle RR, Craig M, LoBuglio AF, Conry RM
JournalClin Cancer Res
PubMed ID15930353
The objective of this study was to evaluate the safety and activity of the intratumoral administration of the immune costimulatory molecule, B7.1, encoded by a vector derived from the canarypox virus, ALVAC (ALVAC-B7.1), alone and with the intratumoral injection of ALVAC encoding the immune-stimulatory cytokine, interleukin 12 (ALVAC-IL-12). Fourteen patients ... More
Optimization of in vitro expansion of human multipotent mesenchymal stromal cells for cell-therapy approaches: further insights in the search for a fetal calf serum substitute.
AuthorsBernardo ME, Avanzini MA, Perotti C, Cometa AM, Moretta A, Lenta E, Del Fante C, Novara F, de Silvestri A, Amendola G, Zuffardi O, Maccario R, Locatelli F
JournalJ Cell Physiol
PubMed ID17187344
There is great interest in mesenchymal stromal cells (MSCs) for cell-therapy and tissue engineering approaches. MSCs are currently expanded in vitro in the presence of fetal calf serum (FCS); however, FCS raises concerns when used in clinical grade preparations. The aim of this study was to evaluate whether MSCs expanded ... More
iC3b arrests monocytic cell differentiation into CD1c-expressing dendritic cell precursors: a mechanism for transiently decreased dendritic cells in vivo after human skin injury by ultraviolet B.
AuthorsTakahara M, Kang K, Liu L, Yoshida Y, McCormick TS, Cooper KD
JournalJ Invest Dermatol
PubMed ID12713585
Our previous data indicated that C3, its bioactive product iC3b, and the iC3b ligand CD11b are critical for ultraviolet-induced immunosuppression. We thus hypothesized that iC3b is an important skin-based factor regulating CD11b+ monocytic cell function in the acute post-ultraviolet period. Although monocytic cell migration peaked at 1-3 d after ultraviolet ... More
A lentivirus-mediated genetic screen identifies dihydrofolate reductase (DHFR) as a modulator of beta-catenin/GSK3 signaling.
AuthorsKlinghoffer RA, Frazier J, Annis J, Berndt JD, Roberts BS, Arthur WT, Lacson R, Zhang XD, Ferrer M, Moon RT, Cleary MA
JournalPLoS One
PubMed ID19727391
The multi-protein beta-catenin destruction complex tightly regulates beta-catenin protein levels by shuttling beta-catenin to the proteasome. Glycogen synthase kinase 3beta (GSK3beta), a key serine/threonine kinase in the destruction complex, is responsible for several phosphorylation events that mark beta-catenin for ubiquitination and subsequent degradation. Because modulation of both beta-catenin and GSK3beta ... More
Shigella flexneri IpaH(7.8) facilitates escape of virulent bacteria from the endocytic vacuoles of mouse and human macrophages.
AuthorsFernandez-Prada CM, Hoover DL, Tall BD, Hartman AB, Kopelowitz J, Venkatesan MM
JournalInfect Immun
PubMed ID10816519
The behavior of Shigella flexneri ipaH mutants was studied in human monocyte-derived macrophages (HMDM), in 1-day-old human monocytes, and in J774 mouse macrophage cell line. In HMDM, strain pWR700, an ipaH(7.8) deletion mutant of S. flexneri 2a strain 2457T, behaved like the wild-type strain 2457T. This strain caused rapid host ... More