Coxsackie B4 virus infection of beta cells and natural killer cell insulitis in recent-onset type 1 diabetic patients.
AuthorsDotta F, Censini S, van Halteren AG, Marselli L, Masini M, Dionisi S, Mosca F, Boggi U, Muda AO, Del Prato S, Elliott JF, Covacci A, Rappuoli R, Roep BO, Marchetti P
JournalProc Natl Acad Sci U S A
PubMed ID17360338
Type 1 diabetes is characterized by T cell-mediated autoimmune destruction of pancreatic beta cells. Several studies have suggested an association between Coxsackie enterovirus seroconversion and onset of disease. However, a direct link between beta cell viral infection and islet inflammation has not been established. We analyzed pancreatic tissue from six ... More
Gestational age-dependent expression of IL-10 and its receptor in human placental tissues and isolated cytotrophoblasts.
AuthorsHanna N, Hanna I, Hleb M, Wagner E, Dougherty J, Balkundi D, Padbury J, Sharma S
JournalJ Immunol
PubMed ID10820249
'Control of antifetal immune responses is thought to be regulated locally by the placenta. Because the physiologic programming of the placenta across gestation is likely to influence the local immunity, we hypothesize that a potent anti-inflammatory cytokine such as IL-10 may be produced in a gestational age-dependent manner. In the ... More
PK1/EG-VEGF induces monocyte differentiation and activation.
AuthorsDorsch M, Qiu Y, Soler D, Frank N, Duong T, Goodearl A, O'Neil S, Lora J, Fraser CC
JournalJ Leukoc Biol
PubMed ID15908459
'Macrophages exist as sentinels in innate immune response and react by expressing proinflammatory cytokines and up-regulating antigen-presenting and costimulatory molecules. We report a novel function for prokineticin-1 (PK1)/endocrine gland-derived vascular endothelial growth factor. Screening of murine tissue sections and cells for specific binding site leads to the identification of macrophages ... More
Human papillomavirus type 16 E6-enhanced susceptibility to apoptosis induced by TNF in A2780 human ovarian cancer cell line.
AuthorsVikhanskaya F, Falugi C, Valente P, Russo P
JournalInt J Cancer
PubMed ID11857347
'In our study, we show that expression of HPV-16 E6 sensitizes TNF-induced cytotoxicity of human ovarian cancer cell line A2780. This effect is not related to a different number of TNF receptors present on cell membrane. The major induction of massive apoptosis induced by TNF is not p53- and p21(waf-1)-dependent ... More
Human monocyte-derived dendritic cells differentiated in the presence of IL-2 produce proinflammatory cytokines and prime Th1 immune response.
AuthorsSanarico N, Ciaramella A, Sacchi A, Bernasconi D, Bossù P, Mariani F, Colizzi V, Vendetti S
JournalJ Leukoc Biol
PubMed ID16809642
'Interleukin (IL)-2 plays an important role in the control of the immune responses, and it is released in a variety of tissues in response to inflammatory stimuli. As monocytes and mature dendritic cells (DCs) express CD25, the high-affinity subunit of IL-2 receptor, we examined the effect of exogenous IL-2 on ... More
Cytokine production and monocyte HLA-DR expression as predictors of outcome for patients with community-acquired severe infections.
AuthorsLekkou A, Karakantza M, Mouzaki A, Kalfarentzos F, Gogos CA
JournalClin Diagn Lab Immunol
PubMed ID14715564
'This study was performed to evaluate the impact of pro- and anti-inflammatory molecules and human leukocyte antigen DR (HLA-DR) expression as markers of immune status for the final outcome of septic patients. The study included 30 patients with severe sepsis due to community-acquired infections. Concentrations of tumor necrosis factor alpha ... More
Human natural killer cells: a unique innate immunoregulatory role for the CD56(bright) subset.
AuthorsCooper MA, Fehniger TA, Turner SC, Chen KS, Ghaheri BA, Ghayur T, Carson WE, Caligiuri MA
JournalBlood
PubMed ID11342442
'During the innate immune response to infection, monocyte-derived cytokines (monokines), stimulate natural killer (NK) cells to produce immunoregulatory cytokines that are important to the host''s early defense. Human NK cell subsets can be distinguished by CD56 surface density expression (ie, CD56(bright) and CD56(dim)). In this report, it is shown that ... More
Monocyte cytokine secretion in patients with pulmonary tuberculosis differs from that of healthy infected subjects and correlates with clinical manifestations.
AuthorsPereira CB, Palaci M, Leite OH, Duarte AJ, Benard G
JournalMicrobes Infect
PubMed ID14738890
'Cell-mediated immunity, leading to Mycobacterium tuberculosis (Mtb)-constraining granuloma formation, is the major component of host defense against tuberculosis and is regulated by the balance of cytokines secreted mostly by mononuclear phagocytes and lymphocytes. To better understand the role of monocytes in the regulation of the immune response against pulmonary tuberculosis, ... More
Phase I trial of TALL-104 cells in patients with refractory metastatic breast cancer.
AuthorsVisonneau S, Cesano A, Porter DL, Luger SL, Schuchter L, Kamoun M, Torosian MH, Duffy K, Sickles C, Stadtmauer EA, Santoli D
JournalClin Cancer Res
PubMed ID10815893
'The human cytotoxic T-cell line TALL-104 displays antitumor effects in animals with implanted and spontaneous malignancies. A Phase I trial was conducted to determine toxicity of TALL-104 cell therapy in women with metastatic refractory breast cancer. Fifteen patients with metastatic infiltrating ductal (n = 12), lobular (n = 2), or ... More
The effect of particle wear debris on NFkappaB activation and pro-inflammatory cytokine release in differentiated THP-1 cells.
AuthorsAkisue T, Bauer TW, Farver CF, Mochida Y
JournalJ Biomed Mater Res
PubMed ID11774309
'Orthopedic wear debris has been thought to be an important factor associated with osteolysis and loosening of total joint arthroplasties. Previous in vitro studies have reported that particles of wear debris induce the release of pro-inflammatory cytokines and other inflammatory mediators from macrophages and other cells. Several recent investigations, however, ... More
In vitro study of malaria parasite induced disruption of blood-brain barrier.
'The mechanism of blood-brain barrier breakdown in the complex pathogenesis of cerebral malaria is not well understood. In this study, primary cultures of porcine brain capillary endothelial cells (PBCEC) were used as in vitro model. Membrane-associated malaria antigens obtained from lysed Plasmodium falciparum schizont-infected erythrocytes stimulated human peripheral blood mononuclear ... More
Normal pressure hydrocephalus triggers intrathecal production of TNF-alpha.
AuthorsTarkowski E, Tullberg M, Fredman P, Wikkelsö C
JournalNeurobiol Aging
PubMed ID12885578
'Normal pressure hydrocephalus (NPH) is associated with periventricular white matter lesions and demyelination. The aim of the present study was to examine the cerebrospinal fluid (CSF) levels of tumor necrosis factor-alpha (TNF-alpha), a proinflammatory cytokine mediating myelin damage, in patients with NPH. TNF-alpha levels were analyzed by ELISA and measured ... More
Endotoxic and immunobiological activities of a chemically synthesized lipid A of Helicobacter pylori strain 206-1.
AuthorsOgawa T, Asai Y, Sakai Y, Oikawa M, Fukase K, Suda Y, Kusumoto S, Tamura T
JournalFEMS Immunol Med Microbiol
PubMed ID12727359
'A synthetic lipid A of Helicobacter pylori strain 206-1 (compound HP206-1), which is similar to its natural lipid A, exhibited no or very low endotoxic activities as compared to Escherichia coli-type synthetic lipid A (compound 506). Furthermore, compound HP206-1 as well as its natural lipid A demonstrated no or very ... More
Involvement of TL1A and DR3 in induction of pro-inflammatory cytokines and matrix metalloproteinase-9 in atherogenesis.
AuthorsKang YJ, Kim WJ, Bae HU, Kim DI, Park YB, Park JE, Kwon BS, Lee WH
JournalCytokine
PubMed ID15760679
'TL1A (VEGI/TNFSF15) is the ligand for DR3 (TNFRSF12) and is a newly identified member of the tumor necrosis factor superfamily (TNFSF). Previously, DR3 has been shown to have a role in atherogenesis through stimulation of matrix degrading enzymes including matrix metalloproteinase (MMP)-9. Immunohistochemical staining of human carotid atherosclerotic plaques revealed ... More
Monoclonal light chain--mesangial cell interactions: early signaling events and subsequent pathologic effects.
AuthorsRussell WJ, Cardelli J, Harris E, Baier RJ, Herrera GA
JournalLab Invest
PubMed ID11351041
'Glomerulopathic monoclonal light chains (G-LC) interact with mesangial cells (MC), resulting in alterations of mesangial homeostasis. Early signaling events control mitogenic activities and cytokine production, which in turn participate in the subsequent pathologic events. Mesangial homeostasis is affected in two very different ways, depending on whether the G-LC is from ... More
Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104.
AuthorsBrando C, Mukhopadhyay S, Kovacs E, Medina R, Patel P, Catina TL, Campbell KS, Santoli D
JournalJ Leukoc Biol
PubMed ID15937142
'The major histocompatibility complex nonrestricted cytotoxic leukemic T cell line T acute lymphoblastic leukemia (TALL)-104 is being pursued as a therapeutic agent for cancer. However, the receptors and effector mechanisms responsible for its broad tumoricidal function remain undefined. Here, we examined the roles played by natural cytotoxicity receptors (NCR), killer ... More
Altered conjugate formation and altered apoptosis of multidrug-resistant human leukemia cell line affects susceptibility to killing by activated natural killer (NK) cells.
AuthorsTreichel RS, Bunuan M, Hahn N, Wee K
JournalInt J Cancer
PubMed ID14618619
'Most leukemias that exhibit P-glycoprotein (P-gp)-associated multidrug resistance (MDR) exhibit reduced susceptibility to immune cytotoxicity mediated by natural killer (NK) cells. To explore this phenomenon we investigated N6/ADR, a doxorubicin-selected, P-gp-positive variant of the human acute lymphoblastic leukemia (ALL) cell line NALM6. Each stage of the NK cytolytic pathway, (binding, ... More
Bystander-killing effect and cyclic induction of TNF-alpha gene under heat-inducible promoter gadd 153.
AuthorsIto A, Shinkai M, Bouhon IA, Honda H, Kobayashi T
JournalJ Biosci Bioeng
PubMed ID16232886
'The bystander-killing effect and cyclic induction of tumor necrosis factor (TNF)-alpha gene expression under the heat-inducible promoter gadd 153 (growth arrest and DNA damage) were investigated. The plasmid harboring the TNF-alpha gene under the control of the gadd 153 promoter (pGadTNF) was introduced into cells of the human glioma cell ... More
Altered nitric oxide/cGMP platelet signaling pathway in platelets from patients with acute coronary syndromes.
AuthorsBergandi L, Cordero M, Anselmino M, Ferraro G, Ravera L, Dalmasso P, Moiraghi C, Trevi GP, Ghigo D, Bosia A, Bergerone S
JournalClin Res Cardiol
PubMed ID20467748
'This study was aimed at evaluating whether the nitric oxide (NO)/cyclic GMP (cGMP) signaling pathway is altered in platelets from patients with an acute coronary syndrome (unstable angina and acute myocardial infarction). We investigated 10 patients with unstable angina (UA), 14 with acute myocardial infarction (AMI) and 14 age and ... More
Selective activation of specific PKC isoforms dictating the fate of CD14(+) monocytes towards differentiation or apoptosis.
AuthorsLin YF, Leu SJ, Huang HM, Tsai YH
JournalJ Cell Physiol
PubMed ID20626007
'In this study, phorbol-12-myristate-13-acetate (PMA) at low concentrations (<10 nM; L-PMA) induces the differentiation of CD14(+) monocytes into monocyte-derived macrophages (MDMs) while PMA at high concentrations (>100 nM; H-PMA) causes the apoptosis of these cells. The pre-treatment with Go6976 (a PKC-a/ß(1) selective inhibitor), not anilinemonoindolylmaleimide [a PKC-ß inhibitor (PKC-ß inh.)], ... More
Critical role of activation-inducible lymphocyte immunomediatory molecule/inducible costimulator in the effector function of human T cells: a comparative in vitro study of effects of its blockade and CD28 blockade in human beings and monkeys.
AuthorsTajima N, Tezuka K, Tanaka M, Tanimoto M, Miyai A, Takeshima H, Watanabe Y
JournalHum Immunol
PubMed ID18638655
'Activation-inducible lymphocyte immunomediatory molecule (AILIM; also referred to as inducible costimulator, ICOS) is the third homolog of the "professional" costimulatory molecule, CD28. To date, the characteristics and role of AILIM/ICOS, especially in effector function of T cells, have been determined through numerous studies in vitro and in vivo using mice. ... More
CD154 activates macrophage antimicrobial activity in the absence of IFN-gamma through a TNF-alpha-dependent mechanism.
AuthorsAndrade RM, Wessendarp M, Subauste CS
JournalJ Immunol
PubMed ID14662879
'Protection against certain intracellular pathogens can take place in the absence of IFN-gamma through mechanisms dependent on TNF-alpha. In this regard, patients with partial defect in IFN-gamma receptor 1 are not susceptible to toxoplasmosis. Thus, we used a model of Toxoplasma gondii infection to investigate whether CD154 modulates IFN-gamma-independent mechanisms ... More
Evaluation of adipose-derived stromal vascular fraction or bone marrow-derived mesenchymal stem cells for treatment of osteoarthritis.
'The purpose of this study was the assessment of clinical, biochemical, and histologic effects of intraarticular administered adipose-derived stromal vascular fraction or bone marrow-derived mesenchymal stem cells for treatment of osteoarthritis. Osteoarthritis was induced arthroscopically in the middle carpal joint of all horses, the contralateral joint being sham-operated. All horses ... More
Relationship of asymmetric dimethylarginine to haemodialysis hypotension.
AuthorsBergamini S, Vandelli L, Bellei E, Rota C, Manfredini P, Tomasi A, Albertazzi A, Iannone A
JournalNitric Oxide
PubMed ID15566974
'Hypotension is one of the major complications in patients undergoing haemodialysis (HD), that is well evident in patients defined as "hypotension-prone." The mechanisms underlying the hypotensive episodes are not known. We carried out a clinical study on hypotension-prone HD patients to test the existence of a dysregulation in the nitric ... More
IL-12 completely blocks ultraviolet-induced secretion of tumor necrosis factor alpha from cultured skin fibroblasts and keratinocytes.
AuthorsWerth VP, Bashir MM, Zhang W
JournalJ Invest Dermatol
PubMed ID12535207
'Interleukin-12 is an important regulator of other cytokines. Although interleukin-12 is considered to act primarily on lymphocytes, provoking a shift from T helper 2 to T helper 1 cells and an increase in lymphocyte-derived tumor necrosis factor alpha, we hypothesized that interleukin-12 might also affect tumor necrosis factor alpha secretion ... More
Eugenol suppressed the expression of lipopolysaccharide-induced proinflammatory mediators in human macrophages.
AuthorsLee YY, Hung SL, Pai SF, Lee YH, Yang SF
JournalJ Endod
PubMed ID17509409
'Eugenol is commonly used as an analgesic agent during acute pulpitis and is a major component of root canal sealers. Despite the frequent applications of eugenol in the practice of dentistry, little is known about the role of eugenol under the status of inflammation. This study was aimed to investigate ... More
Postnatal behavioral and physiological responses of piglets from gilts housed individually or in groups during gestation.
AuthorsSorrells AD, Eicher SD, Scott KA, Harris MJ, Pajor EA, Lay DC, Richert BT
JournalJ Anim Sci
PubMed ID16478968
'Gestational housing of sows remains a controversial issue that may affect the well-being of both sows and piglets. Therefore, 2 types of gestational housing were used to evaluate the stress imposed on pregnant gilts by each system and the effects on the offspring by comparing production, physiology, and behavioral measures ... More
Japanese encephalitis virus envelope protein mitigates TNF-alpha mRNA expression in RAW264.7 cells.
'Japanese encephalitis virus (JEV) is known as an important mosquito-borne human pathogen that causes Japanese encephalitis and may lead to lethal effect. Since monocyte has been demonstrated to play transmissible role for JEV, rare study is reported to clarify the effect of JEV envelope (JEVE) protein on monocyte. This study ... More
Nuclear factor kappaB dependency of platelet-activating factor-induced angiogenesis.
AuthorsKo HM, Seo KH, Han SJ, Ahn KY, Choi IH, Koh GY, Lee HK, Ra MS, Im SY
JournalCancer Res
PubMed ID11912159
'This study investigated the mechanisms of platelet-activating factor (PAF)-induced angiogenesis in a mouse model of Matrigel implantation. PAF induced a dose- and time-dependent angiogenic response. Inhibitors of nuclear factor (NF) kappaB expression or action, including antisense oligonucleotides to the p65 subunit of NFkappaB (p65 antisense) and antioxidants such as alpha-tocopherol ... More
Activation of the complement system as an indicator of pyrogenic reaction to lipopolysaccharide (LPS).
AuthorsSladowski D, Kinsner A, Langezaal I, Kay S, Coecke S
JournalToxicol In Vitro
PubMed ID11566559
'The generation of biologically active complement split products through the direct reaction of microorganisms with complement proteins is one of the earliest events of the defence reaction in humans. Complement activation develops within minutes, which highly corresponds with the onset of a febrile reaction after exposure to pyrogens. The possibility ... More
Differential effect of resuscitation on Toll-like receptors in a model of hemorrhagic shock without a septic challenge.
AuthorsChen H, Koustova E, Shults C, Sailhamer EA, Alam HB
JournalResuscitation
PubMed ID17386967
'It has been shown that the inflammatory response and cellular damage after hemorrhagic shock are influenced by resuscitation strategies. Toll-like receptors (TLRs) play an important role in signal transduction in inflammatory conditions. However, alterations in TLR expression following hemorrhagic shock and resuscitation have not been well documented. This study was ... More
CD40 triggering enhances fludarabine-induced apoptosis of chronic lymphocytic leukemia B-cells through autocrine release of tumor necrosis factor-alpha and interferon-gama and tumor necrosis factor receptor-I-II upregulation.
Authorsde Totero D, Tazzari PL, Capaia M, Montera MP, Clavio M, Balleari E, Foa R, Gobbi M
JournalHaematologica
PubMed ID12604404
'In chronic lymphocytic leukemia (CLL) B-cells are refractory to activation signals and to apoptosis. CD40 triggering, however, rescues CLL B-cells from their anergic state and upregulates the FAS receptor. We therefore studied whether CD40 triggering enhances CLL B-cell sensitivity to fludarabine, and receptors or cytokines potentially involved in apoptosis.' ... More
Troponin as a risk factor for mortality in critically ill patients without acute coronary syndromes.
AuthorsAmmann P, Maggiorini M, Bertel O, Haenseler E, Joller-Jemelka HI, Oechslin E, Minder EI, Rickli H, Fehr T
JournalJ Am Coll Cardiol
PubMed ID12798573
'We sought to assess the mechanism and prognostic value of elevated troponins in patients without acute coronary syndromes (ACS).'
Qualitative immune modulation by interleukin-2 (IL-2) adjuvant therapy in immunological non responder HIV-infected patients.
AuthorsSabbatini F, Bandera A, Ferrario G, Trabattoni D, Marchetti G, Franzetti F, Clerici M, Gori A
JournalPLoS One
PubMed ID21124762
'Treatment of HIV-infected patients with interleukin-2 (IL-2) produces significant increases in CD4 T cell counts; however an associated qualitative improvement in cells function has yet to be conclusively demonstrated. By measuring mycobacterial killing activity, we evaluated IL-2-mediated functional immune enhancement ex vivo in immunological non-responders (INRs).' ... More
Towards an in vitro model for acute hemorrhagic conjunctivitis: cytokine-mediated vascular endothelial cell activation triggered by enterovirus type 70 infection.
AuthorsChang CH, Lin KH, Anderson R
JournalJ Clin Virol
PubMed ID15072749
'Little is known of the pathogenetic mechanisms of enterovirus type 70 (EV70), a causative agent of acute hemorrhagic conjunctivitis. However, virus- or cytokine-induced perturbation of vascular endothelial cells are potential triggering events.' ... More
Analysis of chemokine and cytokine expression in patients with HIV and GB virus type C coinfection.
AuthorsGiménez-Barcons M, Ribera M, Llano A, Clotet B, Esté JA, Martínez MA
JournalClin Infect Dis
PubMed ID15828087
'Plasma levels of several chemokines and cytokines were evaluated in a cohort of 161 human immunodeficiency virus (HIV)positive patients to shed light on a clinically relevant mechanism that would explain the putative beneficial effect of GB virus type C (GBV-C) coinfection.' ... More
The RANK/RANK ligand system is involved in interleukin-6 and interleukin-11 up-regulation by human myeloma cells in the bone marrow microenvironment.
AuthorsGiuliani N, Colla S, Morandi F, Rizzoli V
JournalHaematologica
PubMed ID15377473
'The receptor activator of NF-kB ligand (RANKL) has a critical role in osteoclast activation. Recently it has been demonstrated that human multiple myeloma (MM) cells do not express RANKL but up-regulate RANKL in bone marrow stromal cells (BMSC). To further investigate the role of RANKL in the pathophysiology of MM ... More
Recognition by TNF-alpha of the GPI-anchor glycan induces apoptosis of U937 cells.
AuthorsFukushima K, Ishiyama C, Yamashita K
JournalArch Biochem Biophys
PubMed ID15158680
'Tumor necrosis factor-alpha (TNF-alpha) binds to TNF-alpha receptors (TNFR) to produce a hexameric (TNF-alpha)(3)-(TNFR)(3) structure that stimulates apoptosis. We found by using ELISA that TNF-alpha binds to the glycosylphosphatidylinositol (GPI) anchor glycans of carcinoembryonic antigen, human placental alkaline phosphatase (hAP), and Tamm-Horsfall glycoprotein. These binding abilities were inhibited by 10(-6)M ... More
Advanced oxidation protein products in obese women: its relation to insulin resistance and resistin.
AuthorsKoçak H, Oner-Iyidogan Y, Gürdöl F, Oner P, Süzme R, Esin D, Issever H
JournalClin Exp Med
PubMed ID18188531
'Obesity is a major risk factor for insulin resistance and type 2 diabetes mellitus (T2DM). Resistin, an adipocyte-secreted hormone, is thought to take a part in the development of insulin resistance and T2DM. The aim of this study was to characterise the changes in circulating levels of resistin and proinflammatory ... More
Self-heat shock protein 60 induces tumour necrosis factor-alpha in monocyte-derived macrophage: possible role in chronic inflammatory periodontal disease.
AuthorsUeki K, Tabeta K, Yoshie H, Yamazaki K
JournalClin Exp Immunol
PubMed ID11882035
'Heat shock protein 60 (hsp60) has been increasingly recognized as an important molecule in infectious and autoimmune diseases. We have demonstrated previously that serum antibodies to both human hsp60 and Porphyromonas gingivalis GroEL were elevated in periodontitis patients compared with healthy subjects. In order to clarify the relative importance of ... More
Effect of advanced glycation end-products on gene expression and synthesis of TNF-alpha and endothelial nitric oxide synthase by endothelial cells.
'Advanced glycation end-products (AGEs), which are formed in aging, diabetes mellitus, and kidney failure are implicated in the occurrence of vascular complications. We, thus, evaluated in cultured endothelial cells, the AGEs'' effect on gene expression and synthesis of tumor necrosis factor-alpha (TNF-alpha) and endothelial nitric oxide synthase (eNOS) mRNA and ... More
Retroviral transfer of T-cell receptor genes into human peripheral blood lymphocytes.
AuthorsClay TM, Nishimura MI
JournalMethods Mol Biol
PubMed ID12512302
Extravasation into synovial tissue induces CCL20 mRNA expression in polymorphonuclear neutrophils of patients with rheumatoid arthritis.
AuthorsSchlenk J, Lorenz HM, Haas JP, Herrmann M, Hohenberger G, Kalden JR, Röllinghoff M, Beuscher HU
JournalJ Rheumatol
PubMed ID16331752
Examination of expression of the chemokine macrophage inflammatory protein-3a (CCL20/Mip-3alpha) in blood polymorphonuclear neutrophils (PMN) and synovial fluid (SF) PMN of patients with rheumatoid arthritis (RA). ... More
Complement activation in patients with primary antiphospholipid syndrome.
AuthorsOku K, Atsumi T, Bohgaki M, Amengual O, Kataoka H, Horita T, Yasuda S, Koike T
JournalAnn Rheum Dis
PubMed ID18625630
To investigate the significance of complement activation in patients with primary antiphospholipid syndrome (APS).
A randomized prospective trial of low-dose OKT3 induction therapy to prevent rejection and minimize side effects in recipients of kidney transplants.
AuthorsFlechner SM, Goldfarb DA, Fairchild R, Modlin CS, Fisher R, Mastroianni B, Boparai N, O'Malley KJ, Cook DJ, Novick AC
JournalTransplantation
PubMed ID10868643
We attempted to minimize the undesired side effects and maximize the benefit of OKT3 induction therapy in renal transplantation.
Abacavir hypersensitivity reaction in primary HIV infection.
AuthorsStekler J, Maenza J, Stevens C, Holte S, Malhotra U, McElrath MJ, Corey L, Collier AC
JournalAIDS
PubMed ID16816555
To evaluate risk factors associated with the abacavir hypersensitivity reaction during primary HIV infection (PHI).
Soluble Fas ligand and soluble Fas in ocular fluid of patients with uveitis.
AuthorsSugita S, Taguchi C, Takase H, Sagawa K, Sueda J, Fukushi K, Hikita N, Watanabe T, Itoh K, Mochizuki M
JournalBr J Ophthalmol
PubMed ID11004098
To investigate the presence of soluble Fas ligand (sFasL) and soluble Fas (sFas) in ocular fluid of patients with uveitis.
Allergen-induced cytokine production, atopic disease, IgE, and wheeze in children.
AuthorsContreras JP, Ly NP, Gold DR, He H, Wand M, Weiss ST, Perkins DL, Platts-Mills TA, Finn PW
JournalJ Allergy Clin Immunol
PubMed ID14657861
The early childhood allergen-induced immune responses associated with atopic disease and IgE production in early life are not well understood.
Insulin treatment improves the systemic inflammatory reaction to severe trauma.
AuthorsJeschke MG, Klein D, Herndon DN
JournalAnn Surg
PubMed ID15024317
Determine the effect of insulin on the systemic inflammatory response, pro- and anti-inflammatory cytokines and hepatic acute-phase-response in severely burned pediatric patients.
Concentrations of gastric mucosal cytokines in children with food allergy and Helicobacter pylori infection.
AuthorsMaciorkowska E, Panasiuk A, Kaczmarsk M
JournalWorld J Gastroenterol
PubMed ID16425379
To measure the concentrations of chosen cytokines in the antrum mucosa depending on the kind of harmful pathogenic factors and to compare the concentrations with the values of controls without allergy and coexisting Helicobacter pylori (H pylori) infection. ... More
Absence of mucosal inflammation in uncomplicated diverticular disease.
AuthorsElli L, Roncoroni L, Bardella MT, Terrani C, Bonura A, Ciulla M, Marconi S, Piodi L
JournalDig Dis Sci
PubMed ID21221786
Uncomplicated diverticular disease is a common condition in patients older than 50 years. Symptoms are aspecific and overlapping with those of irritable bowel syndrome. Nowadays, patients are often treated with antinflammatory drugs (5-aminosalicilic acid). ... More
T-helper 2 and 3 type immunity to trophoblast in successful in vitro fertilization-embryo transfer.
AuthorsGinsburg ES, Xiao L, Gargiulo AR, Kung FT, Politch JA, Schust DJ, Hill JA
JournalFertil Steril
PubMed ID15950633
To determine whether peripheral blood mononuclear cell (PBMC) secretion of T-helper (Th)-1 type cytokines and Th-2 and Th-3 type cytokines in women undergoing in vitro fertilization-embryo transfer (IVF-ET) is associated with therapeutic failure and success, respectively. ... More
Prognostic predictive values of serum cytochrome c, cytokines, and other laboratory measurements in acute encephalopathy with multiple organ failure.
AuthorsHosoya M, Kawasaki Y, Katayose M, Sakuma H, Watanabe M, Igarashi E, Aoyama M, Nunoi H, Suzuki H
JournalArch Dis Child
PubMed ID16443616
To evaluate the prognostic predictive values of cytochrome c, cytokines, and other laboratory measurements in serum collected during neurological onset in acute encephalopathy with multiple organ failure. ... More
NCX-4016 (NO-aspirin) inhibits lipopolysaccharide-induced tissue factor expression in vivo: role of nitric oxide.
AuthorsFiorucci S, Mencarelli A, Meneguzzi A, Lechi A, Morelli A, del Soldato P, Minuz P
JournalCirculation
PubMed ID12473561
NCX-4016 is an acetylsalicylic acid (ASA) derivative containing a nitric oxide-releasing moiety. Compared with ASA, NCX-4016 has a broader spectrum of antithrombotic and antiinflammatory activities. We hypothesized that NCX-4016 might inhibit in vivo lipopolysaccharide (LPS)-induced expression of tissue factor (TF). ... More
Differential induction of apoptosis and necrosis in monocytes from patients with tuberculosis and healthy control subjects.
AuthorsGil DP, León LG, Correa LI, Maya JR, París SC, García LF, Rojas M
JournalJ Infect Dis
PubMed ID15143481
Mycobacterium tuberculosis and purified protein derivative (PPD) induce apoptosis in murine macrophages and apoptosis and necrosis in human monocytes and alveolar epithelial cells. Macrophages from bronchoalveolar lavages and granulomas from patients with tuberculosis (TB) present both types of cell death; however, the significance of the type of cell death in ... More
Adenosine A2A receptor activation reduces inflammation and preserves pulmonary function in an in vivo model of lung transplantation.
Reperfusion injury continues to significantly affect patients undergoing lung transplantation. Isolated lung models have demonstrated that adenosine A 2A receptor activation preserves function while decreasing inflammation. We hypothesized that adenosine A 2A receptor activation by ATL-146e during the initial reperfusion period preserves pulmonary function and attenuates inflammation in a porcine ... More
Binding of receptor for advanced glycation end products (RAGE) ligands is not sufficient to induce inflammatory signals: lack of activity of endotoxin-free albumin-derived advanced glycation end products.
AuthorsValencia JV, Mone M, Koehne C, Rediske J, Hughes TE
JournalDiabetologia
PubMed ID15127201
Activation of the receptor for advanced glycation end products (RAGE) reportedly triggers cellular responses implicated in the pathogenesis of diabetes, such as increasing vascular cell adhesion molecule-1 (VCAM-1) expression on vascular endothelial cells and inducing TNF-alpha secretion by mononuclear cells. The objective of this study was to evaluate whether RAGE ... More
Raised serum interleukin 15 levels in Kawasaki disease.
AuthorsJang GC, Kim HY, Ahn SY, Kim DS
JournalAnn Rheum Dis
PubMed ID12594117
Interleukin (IL)15 is a novel cytokine that induces T cell proliferation, B cell maturation, natural killer cell cytotoxicity, and may have a pivotal role in the pathogenesis of inflammatory disease, acting upstream from tumour necrosis factor alpha (TNFalpha). Kawasaki disease (KD) is an inflammatory disease, in which serum levels of ... More
Plasma and urine levels of urinary trypsin inhibitor in patients with acute and fulminant hepatitis.
AuthorsLin SD, Endo R, Sato A, Takikawa Y, Shirakawa K, Suzuki K
JournalJ Gastroenterol Hepatol
PubMed ID11966943
Urinary trypsin inhibitor (UTI) is synthesized by hepatocytes and excreted into urine. Plasma and urine UTI levels have been measured to evaluate whether these levels may be useful markers in various pathological conditions. However, there has been no study on plasma and urine UTI levels in patients with acute liver ... More
Modulation of monocytic lipopolysaccharide-induced tissue factor expression and tumor necrosis factor alpha release by estrogen and calcitriol.
AuthorsVlotides G, Gaertner R, Angstwurm MW
JournalHormones (Athens)
PubMed ID17324918
Modulatory effects of estrogens on both the immune and the coagulation system are only partially understood. In severe infections high estrogen levels have been observed both in men and postmenopausal women and are associated with increased mortality. Monocyte-derived tissue factor (TF) expression can activate the coagulation system and worsen the ... More
Advanced glycation end products stimulate tumor necrosis factor-alpha and interleukin-1 beta secretion by peritoneal macrophages in patients on continuous ambulatory peritoneal dialysis.
AuthorsRashid G, Korzets Z, Bernheim J
JournalIsr Med Assoc J
PubMed ID16450750
Advanced glycation end products, formed by the non-enzymatic glycation of proteins with reducing sugars, are thought to play a pathogenetic role in the vascular complications of diabetes, uremia and atherosclerosis. 132-microglobulin is a major constituent of amyloid fibrils in dialysis-related amyloidosis. AGE1-modified beta2m has been found in amyloid deposits of ... More
Continuous pharmacodynamic activity of eritoran tetrasodium, a TLR4 antagonist, during intermittent intravenous infusion into normal volunteers.
AuthorsRossignol DP, Wong N, Noveck R, Lynn M
JournalInnate Immun
PubMed ID19039062
Eritoran tetrasodium (E5564), a structural analogue of the lipid A portion of endotoxin (lipopolysaccharide or LPS), is an antagonist of LPS and other Toll-like receptor 4 (TLR4) ligands. Eritoran tetrasodium quantitatively blocks LPS response in vivo in animal and human endotoxemia models and demonstrates a long pharmacokinetic half-life, but a ... More
The pattern of a T(H)1 cytokine in autoimmune thyroiditis.
AuthorsDrugarin D, Negru S, Koreck A, Zosin I, Cristea C
JournalImmunol Lett
PubMed ID10714432
This study performed on 51 patients with autoimmune thyroiditis and 15 healthy subjects was aimed at correlating the activation of T cells and the secretion of inflammatory cytokine with echography and thyroid functional assays. A significant increase of activated T cells was observed in Hashimoto patients illustrated by an increased ... More
Human dendritic cells engineered to express alpha tumor necrosis factor maintain cellular maturation and T-cell stimulation capacity.
AuthorsYe Z, Chen Z, Sami A, El-Gayed A, Xiang J,
JournalCancer Biother Radiopharm
PubMed ID17257077
Dendritic cell (DC) vaccine has been demonstrated to induce antitumor immunity in animal models. It has been shown that the efficiency of antitumor immunity by DC vaccine is closely correlated with DC maturation status. The mature human DCs generated from peripheral blood mononuclear cells (PBMCs) in the presence of granulocyte ... More
Virus replication and cytokine production in dengue virus-infected human B lymphocytes.
AuthorsLin YW, Wang KJ, Lei HY, Lin YS, Yeh TM, Liu HS, Liu CC, Chen SH
JournalJ Virol
PubMed ID12414963
Dengue virus (DV) replication, antibody-enhanced viral infection, and cytokine responses of human primary B lymphocytes (cells) were characterized and compared with those of monocytes. The presence of a replication template (negative-strand RNA intermediate), viral antigens including core and nonstructural proteins, and increasing amounts of virus with time postinfection indicated that ... More
The regulation of rhinovirus infection in vitro by IL-8, HuIFN-alpha, and TNF-alpha.
AuthorsBerg K, Andersen H, Owen TC
JournalAPMIS
PubMed ID15153159
The influence of three important cytokines (IL-8, TNF-alpha, and HuIFN-alpha) on ongoing rhinovirus infections has been examined in vitro, individually or as combinations. TNF-alpha was able to transform traces of HRV infections into full-blown infections. Furthermore, TNF-alpha was able to down-regulate the antiviral action of HuIFN-alpha completely, even at levels ... More
Single-nucleotide polymorphism in the CD14 promoter and periodontal disease expression in a Japanese population.
AuthorsYamazaki K, Ueki-Maruyama K, Oda T, Tabeta K, Shimada Y, Tai H, Nakajima T, Yoshie H, Herawati D, Seymour GJ
JournalJ Dent Res
PubMed ID12885845
It has been reported that there is a relationship between a single-nucleotide polymorphism (SNP) in the promoter region of the CD14 gene at position -159 (C-->T) and infectious diseases. The aim of the present study was to test the hypthesis that expression of this SNP correlates with periodontal disease in ... More
Elucidating in vitro cell-cell interaction using a microfluidic coculture system.
AuthorsWei CW, Cheng JY, Young TH
JournalBiomed Microdevices
PubMed ID16491333
This work presents a novel microfluidic coculture system that improves the accuracy of evaluating the interaction between cocultured cell types. A microfluidic coculture chip, fabricated by CO(2) laser direct-writing on polymethyl methacrylate (PMMA), was designed to separate two cell types using a microchannel, while permitting transfer of cellular media. The ... More
Radiation-inducible TNF-alpha gene expression under stress-inducible promoter gadd 153 for cancer therapy.
AuthorsIto A, Shinkai M, Hakamada K, Honda H, Kobayashi T
JournalJ Biosci Bioeng
PubMed ID16233154
We demonstrated the effectiveness of radiation-inducible expression of the TNF-alpha gene for cancer therapy in vitro. The TNF-alpha gene under the control of the stress-inducible promoter, gadd 153, was introduced into the human glioma cell line, U251-SP. Without cobalt-60 gamma irradiation, no cytotoxicity against the transfected cells was observed. When ... More
Dengue-specific T cell responses in peripheral blood mononuclear cells obtained prior to secondary dengue virus infections in Thai schoolchildren.
AuthorsMangada MM, Endy TP, Nisalak A, Chunsuttiwat S, Vaughn DW, Libraty DH, Green S, Ennis FA, Rothman AL
JournalJ Infect Dis
PubMed ID12085313
Children who experience secondary dengue virus (DV) infections are at increased risk for dengue hemorrhagic fever. To study the effect of preexisting T cell responses to DV on the severity of secondary virus infection, peripheral blood mononuclear cells (PBMC) from 10 subsequently hospitalized and 12 nonhospitalized Thai schoolchildren were stimulated ... More
Proinflammatory factors in saliva as possible markers for periodontal disease.
AuthorsAurer A, Jorgic-Srdjak K, Plancak D, Stavljenic-Rukavina A, Aurer-Kozelj J
JournalColl Antropol
PubMed ID16417140
Studies have indicated that host inflammatory proteins, enzymes and indicators of bone metabolism present in saliva differ in different types of periodontal disease. However, the number of markers analyzed was limited and the effect of edentulousness was not examined. We measured the concentration of host inflammatory proteins: C-reactive protein (CRP), ... More
Identification of a carcinoembryonic antigen binding protein on monocytes.
AuthorsGanguly A, Yeltsin E, Robbins J
JournalBiochem Biophys Res Commun
PubMed ID14592416
Tumor-associated monocyte/macrophage cells are important stromal components involved in tumor development. A protein on human monocyte is identified that binds to carcinoembryonic antigen (CEA), a glycoprotein overexpressed in colon tumors. This implicates a role for this protein in CEA processing and establishes a link between monocytes and colon tumor cells. ... More
Non-pathogenic Mycobacterium smegmatis induces the differentiation of human monocytes directly into fully mature dendritic cells.
AuthorsMartino A, Sacchi A, Volpe E, Agrati C, De Santis R, Pucillo LP, Colizzi V, Vendetti S
JournalJ Clin Immunol
PubMed ID16133993
Mycobacterium smegmatis infects human monocytes that can be precursors of dendritic cells. We tested whether the interaction of M. smegmatis with monocytes modulated their differentiation into dendritic cells. We found that M. smegmatis-infected monocytes differentiated into CD1a-CCR7+ dendritic cells in the presence of GM-CSF and IL-4 and acquired a mature ... More
High level expression of the bioactive human interleukin-10 in milk of transgenic mice.
AuthorsSohn BH, Chang HG, Kang HS, Yoon H, Bae YS, Lee KK, Kim SJ
JournalJ Biotechnol
PubMed ID12770500
Human interleukin-10 (hIL-10) has wide spectrum of anti-inflammatory activities and has shown a potential to be used for treatment of inflammatory or immune illness. In this study, transgenic mice that over-express human interleukin-10 (IL-10) in their milk were generated using a bovine beta-casein/human IL-10 hybrid gene. After cloning of the ... More
Cytokines in children with otitis media with effusion.
AuthorsSkotnicka B, Hassmann E
JournalEur Arch Otorhinolaryngol
PubMed ID10993552
We assayed 38 middle ear effusions from 23 children aged 4-13 years (mean 7) undergoing tympanostomy tube placements. All fluid was assayed for tumor necrosis factor (TNF) alpha, interleukin (IL) 1beta, IL-8, and IL-10. Cytokine concentrations were measured by means of an enzyme-linked immunosorbent assay. Detectable levels of IL-1beta, IL-8, ... More
A possible role of NF-kappaB and HSP72 in skeletal muscle hypertrophy induced by heat stress in rats.
AuthorsOhno Y, Yamada S, Sugiura T, Ohira Y, Yoshioka T, Goto K
JournalGen Physiol Biophys
PubMed ID20817947
Effects of heat stress on phosphorylated nuclear factor-kappaB (phospho-NF-kappaB) and tumor necrosis factor alpha (TNFalpha) contents in skeletal muscles were studied. Male Wistar rats (7-week-old) were randomly assigned to control and heat-stressed groups. Rats in heat-stressed group were exposed to heat stress (42 degrees C for 60 min) in an ... More
Regulatory T cells generated during cytomegalovirus in vitro stimulation of mononuclear cells from HIV-infected individuals on HAART correlate with decreased lymphocyte proliferation.
AuthorsJesser RD, Li S, Weinberg A
JournalVirology
PubMed ID16782163
HIV-infected patients fail to fully recover cell-mediated immunity despite HAART. To identify regulatory factors, we studied the phenotype and function of in vitro cytomegalovirus (CMV)-stimulated T cells from HAART recipients. CFSE-measured proliferation showed CD4+ and CD8+ cells dividing in CMV-stimulated cultures. Compared with healthy controls, CMV-stimulated lymphocytes from HAART recipients ... More
Activation of vascular endothelial cells by IL-1alpha released by epithelial cells infected with respiratory syncytial virus.
AuthorsChang CH, Huang Y, Anderson R
JournalCell Immunol
PubMed ID12742380
Although pulmonary inflammation is a serious, sometimes life-threatening, consequence of respiratory syncytial virus (RSV) infection, the mechanisms involved are not well understood. Since the process of inflammation is initiated by a complex series of events including the activation of specific adhesion molecules on vascular endothelium, we searched for endothelial cell-activating ... More
Linkage and association analysis of candidate genes for TB and TNFalpha cytokine expression: evidence for association with IFNGR1, IL-10, and TNF receptor 1 genes.
Tuberculosis (TB) is a growing public health threat globally and several studies suggest a role of host genetic susceptibility in increased TB risk. As part of a household contact study in Kampala, Uganda, we have taken a unique approach to the study of genetic susceptibility to TB by developing an ... More
Prostaglandin E2 is a negative regulator on human plasmacytoid dendritic cells.
AuthorsSon Y, Ito T, Ozaki Y, Tanijiri T, Yokoi T, Nakamura K, Takebayashi M, Amakawa R, Fukuhara S
JournalImmunology
PubMed ID16762028
Prostaglandin E2 (PGE2), a major lipid derived from the metabolism of arachidonic acid, is an environmentally bioactive substance produced by inflammatory processes and acts as a cAMP up-regulator that plays an important role in immune responses. It has been reported that PGE2 has the ability to inhibit the production of ... More
Dietary antioxidants and behavioral enrichment enhance neutrophil phagocytosis in geriatric Beagles.
AuthorsHall JA, Picton RA, Finneran PS, Bird KE, Skinner MM, Jewell DE, Zicker S
JournalVet Immunol Immunopathol
PubMed ID16806493
The study objective was to determine the effects of feeding food enriched in antioxidants and a program of environmental/cognitive enrichment on selected ex vivo assays of inflammatory and immune cells in healthy geriatric Beagle dogs (n=21). Four groups of dogs were tested using a 2 x 2 factorial design. The ... More
Laboratory characteristics of acute encephalopathy with multiple organ dysfunctions.
AuthorsMorita H, Hosoya M, Kato A, Kawasaki Y, Suzuki H
JournalBrain Dev
PubMed ID16198204
To clarify the laboratory characteristics and deduce the pathogenesis of acute encephalopathy associated with multiple organ dysfunctions in Japan. We measured cytokine levels [tumor necrosis factor alpha (TNF-alpha), soluble tumor necrosis factor-receptor 1 (sTNF-R1), and interleukin-6 (IL-6)] in serum and cerebrospinal fluid (CSF) as well as general laboratory examinations in ... More
Heat shock-induced matrix metalloproteinase (MMP)-1 and MMP-3 are mediated through ERK and JNK activation and via an autocrine interleukin-6 loop.
AuthorsPark CH, Lee MJ, Ahn J, Kim S, Kim HH, Kim KH, Eun HC, Chung JH
JournalJ Invest Dermatol
PubMed ID15610507
Although many studies have been performed to elucidate the molecular consequences of ultraviolet irradiation, little is known about the effect of infrared radiation on skin aging. In addition to photons, heat is likely to be generated as a consequence of infrared irradiation, and heat shock is widely considered to be ... More
Low interleukin-10 production is associated with diabetes in HIV-infected patients undergoing antiviral therapy.
AuthorsTrabattoni D, Schenal M, Cesari M, Castelletti E, Pacei M, Goldberg B, Gori A, Clerici M
JournalMed Microbiol Immunol
PubMed ID16437243
Reduced interleukin-10 (IL-10) production is associated with type 2 diabetes in elderly individuals. Antiviral therapy (ARV)-induced immune modulation results in diminished IL-10 production, and diabetes can be observed in ARV-treated human immunodeficiency virus (HIV)-infected individuals. We analyzed, in a cross-sectional pilot study, HIV-antigen-stimulated IL-10 and tumor necrosis factor alpha (TNFalpha) ... More
Combination of human tumor necrosis factor-alpha (hTNF-alpha) gene delivery with gemcitabine is effective in models of pancreatic cancer.
Pancreatic adenocarcinoma is an aggressive and highly lethal malignancy. Currently, gemcitabine is commonly used in patients with pancreatic cancer. However, the life expectancy of pancreatic cancer patients remains poor. We explored the possibility of increased anti-tumor activity by combining human tumor necrosis factor-alpha (hTNF-alpha) with current front-line therapy. Human TNF-alpha ... More
Monocytes promote natural killer cell interferon gamma production in response to the endogenous danger signal HMGB1.
AuthorsDeMarco RA, Fink MP, Lotze MT
JournalMol Immunol
PubMed ID15607795
Substantial attention has been paid to the role of the toll-like receptor (TLR) ligands of late and their role in regulating the innate immune response. They serve as exogenous danger signals important in informing and driving the distal adaptive immune response to pathogens. Less clear has been the role of ... More
Prolonged tomato juice consumption has no effect on cell-mediated immunity of well-nourished elderly men and women.
AuthorsWatzl B, Bub A, Blockhaus M, Herbert BM, Lührmann PM, Neuhäuser-Berthold M, Rechkemmer G
JournalJ Nutr
PubMed ID10867042
The immunomodulatory potential of carotenoids has been investigated thoroughly only for beta-carotene. Data on the immunomodulatory activity of other carotenoids such as lycopene are scarce. The objective of this study was to investigate the effects of prolonged tomato juice consumption on cell-mediated immunity of well-nourished healthy elderly persons. In an ... More
Human myeloma cells stimulate the receptor activator of nuclear factor-kappa B ligand (RANKL) in T lymphocytes: a potential role in multiple myeloma bone disease.
AuthorsGiuliani N, Colla S, Sala R, Moroni M, Lazzaretti M, La Monica S, Bonomini S, Hojden M, Sammarelli G, Barillè S, Bataille R, Rizzoli V
JournalBlood
PubMed ID12393684
The biologic mechanisms involved in the pathogenesis of multiple myeloma (MM) bone disease are not completely understood. Recent evidence suggests that T cells may regulate bone resorption through the cross-talk between the critical osteoclastogenetic factor, receptor activator of nuclear factor-kappaB ligand (RANKL), and interferon gamma (IFN-gamma) that strongly suppresses osteoclastogenesis. ... More
High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1.
AuthorsIwata H, Soga Y, Meguro M, Yoshizawa S, Okada Y, Iwamoto Y, Yamashita A, Takashiba S, Nishimura F
JournalJ Endotoxin Res
PubMed ID17956941
Diabetic subjects are susceptible to atherosclerosis. It has been postulated that inflammation plays a crucial role in atherogenesis. Since previous studies suggested persistent low-grade infection by Gram-negative bacteria such as Chlamydia spp. and/or periodontal infection is associated with increased atherogenesis among diabetic subjects, we hypothesized that macrophages under hyperglycemia respond ... More
Correlation between eosinophils and protection against reinfection with Schistosoma mansoni and the effect of human immunodeficiency virus type 1 coinfection in humans.
Longitudinal investigations of an adult male population of Kenyan car washers who have heavy and quantifiable occupational exposure to Schistosoma mansoni cercariae revealed that some individuals develop resistance to reinfection while others remain highly susceptible. We sought to characterize immune correlates associated with host protection in this population. Previous studies ... More
CD40-mediated signaling in monocytic cells: up-regulation of tumor necrosis factor receptor-associated factor mRNAs and activation of mitogen-activated protein kinase signaling pathways.
AuthorsPearson LL, Castle BE, Kehry MR
JournalInt Immunol
PubMed ID11222496
The biochemical pathways involved in CD40 signaling have been extensively studied in B cells and B cell lines, and appear to be primarily initiated by recruitment of the tumor necrosis factor (TNF) receptor-associated factor (TRAF) signaling proteins to the CD40 cytoplasmic domain. Signaling pathways activated through CD40 in monocytes/macrophages have ... More
Substance P antagonist (CP-96,345) inhibits HIV-1 replication in human mononuclear phagocytes.
AuthorsLai JP, Ho WZ, Zhan GX, Yi Y, Collman RG, Douglas SD
JournalProc Natl Acad Sci U S A
PubMed ID11274418
Substance P (SP) is a potent modulator of neuroimmunoregulation. We recently reported that human immune cells express SP and its receptor. We have now investigated the possible role that SP and its receptor plays in HIV infection of human mononuclear phagocytes. SP enhanced HIV replication in human blood-isolated mononuclear phagocytes, ... More
Activation of selective transcription factors and cytokines by water-soluble extract from Lentinus lepideus.
AuthorsJin M, Jung HJ, Choi JJ, Jeon H, Oh JH, Kim B, Shin SS, Lee JK, Yoon K, Kim S
JournalExp Biol Med (Maywood)
PubMed ID12773708
We isolated a water-soluble extract, PG101, from cultured mycelia of Lentinus lepideus. Treatment of human peripheral blood mononuclear cells (PBMCs) with PG101 increased levels of TNF-alpha, IL-1beta, IL-10, and IL-12 by 100- to 1000-fold, whereas GM-CSF and IL-18 were activated by an order of magnitude. On the contrary, IFN-gamma and ... More
The identification of a small molecule inhibitor that specifically reduces T cell-mediated adaptive but not LPS-mediated innate immunity by T cell membrane-monocyte contact bioassay.
AuthorsLi YY, Bao M, Meurer J, Skuballa W, Bauman JG, Doecke WD, Zollner TM
JournalImmunol Lett
PubMed ID18241931
Proinflammatory cytokines such as TNFalpha and IL-1beta are produced in lesional skin of chronic plaque psoriasis patients, and at other sites of chronic inflammation such as arthritic joints. They play vital roles in maintaining inflammation. It has recently been suggested that activated T cell contact-mediated monocyte activation, leading to the ... More
Molecular mechanisms underlying mancozeb-induced inhibition of TNF-alpha production.
AuthorsCorsini E, Viviani B, Birindelli S, Gilardi F, Torri A, Codecà I, Lucchi L, Bartesaghi S, Galli CL, Marinovich M, Colosio C
JournalToxicol Appl Pharmacol
PubMed ID16112155
Mancozeb, a polymeric complex of manganese ethylenebisdithiocarbamate with zinc salt, is widely used in agriculture as fungicide. Literature data indicate that ethylenebisdithiocarbamates (EBDTCs) may have immunomodulatory effects in humans. We have recently found in agricultural workers occupationally exposed to the fungicide mancozeb a statistically significant decrease in lipopolysaccharide (LPS)-induced tumor ... More
Chemically modified tetracycline prevents the development of septic shock and acute respiratory distress syndrome in a clinically applicable porcine model.
AuthorsSteinberg J, Halter J, Schiller H, Gatto L, Carney D, Lee HM, Golub L, Nieman G
JournalShock
PubMed ID16205320
Sepsis causes more than with 215,000 deaths per year in the United States alone. Death can be caused by multiple system organ failure, with the lung, in the form of the acute respiratory distress syndrome (ARDS), often being the first organ to fail. We developed a chronic porcine model of ... More
Tumour necrosis factor-alpha processing in interstitial lung disease: a potential role for exogenous proteinase-3.
AuthorsArmstrong L, Godinho SI, Uppington KM, Whittington HA, Millar AB
JournalClin Exp Immunol
PubMed ID19292764
Tumour necrosis factor (TNF) blockade has become an important immunomodulatory therapy, particularly in patients refractory to conventional immunosuppression, but responses can be unpredictable. Understanding the complex biology of TNF processing may be key to predicting such responses and reduce unwanted side effects. TNF bioavailability is regulated partly by TNF-alpha converting ... More
Differential gene expression profiles of human monocyte-derived antigen presenting cells in response to Penicillium marneffei: roles of DC-SIGN (CD209) in fungal cell uptake.
AuthorsNgaosuwankul P, Pongtanalert P, Engering A, Chaiyaroj SC
JournalAsian Pac J Allergy Immunol
PubMed ID19054934
DNA microarray technology was used to determine the gene expression profile of human monocyte-derived macrophages (hMDMs) after stimulation by Penicillium marneffei yeast. The expression levels of 175 macrophage genes were found to be altered by a minimum of two-fold in magnitude following 4 hours of P. marneffei exposure. Among those, ... More
Generation of low-toxicity interleukin-2 fusion proteins devoid of vasopermeability activity.
AuthorsHu P, Mizokami M, Ruoff G, Khawli LA, Epstein AL
JournalBlood
PubMed ID12609842
Because of its key role in immunity, interleukin-2 (IL-2) has been studied extensively for the adoptive immunotherapy of cancer. Although systemic administration of IL-2 has been shown to stimulate antitumor responses in vivo, its efficacy in the clinic has been limited by the development of serious side effects, including the ... More
Arginine-specific protease from Porphyromonas gingivalis activates protease-activated receptors on human oral epithelial cells and induces interleukin-6 secretion.
Periodontitis is a chronic inflammatory disease affecting oral tissues. Oral epithelial cells represent the primary barrier against bacteria causing the disease. We examined the responses of such cells to an arginine-specific cysteine proteinase (RgpB) produced by a causative agent of periodontal disease, Porphyromonas gingivalis. This protease caused an intracellular calcium ... More