Dendritic cells efficiently acquire and present antigen derived from lung cancer cells and induce antigen-specific T-cell responses.
AuthorsZhou Y, McEarchern JA, Howard E, Pestano G, Salgaller ML, Bosch ML
JournalCancer Immunol Immunother
PubMed ID12835918
'Active immunotherapy of cancer requires the availability of a source of tumor antigens. To date, no such antigen associated with lung cancer has been identified. We have therefore investigated the ability of dendritic cells (DC) to capture whole irradiated human lung tumor cells and to present a defined surrogate antigen ... More
Human monocyte-derived dendritic cells differentiated in the presence of IL-2 produce proinflammatory cytokines and prime Th1 immune response.
AuthorsSanarico N, Ciaramella A, Sacchi A, Bernasconi D, Bossù P, Mariani F, Colizzi V, Vendetti S
JournalJ Leukoc Biol
PubMed ID16809642
'Interleukin (IL)-2 plays an important role in the control of the immune responses, and it is released in a variety of tissues in response to inflammatory stimuli. As monocytes and mature dendritic cells (DCs) express CD25, the high-affinity subunit of IL-2 receptor, we examined the effect of exogenous IL-2 on ... More
Human monocyte-derived and CD83(+) blood dendritic cells enhance NK cell-mediated cytotoxicity.
AuthorsNishioka Y, Nishimura N, Suzuki Y, Sone S
JournalEur J Immunol
PubMed ID11536161
'Dendritic cells (DC) are known to be the most potent APC and to stimulate antigen-specific T cell responses. Recently it was reported that murine DC were also capable of modulating the innate immunity by stimulating NK cells through cell-to-cell contact. In the present study, we examined whether human DC could ... More
Altered phenotype and function of dendritic cells in children with type 1 diabetes.
AuthorsAngelini F, Del Duca E, Piccinini S, Pacciani V, Rossi P, Manca Bitti ML
JournalClin Exp Immunol
PubMed ID16232222
'The importance of dendritic cells (DC) in the activation of T cells and in the maintenance of self-tolerance is well known. We investigated whether alterations in phenotype and function of DC may contribute to the pathogenesis of Type 1 diabetes (T1DM). Mature DC (mDC) from 18 children with T1DM and ... More
Freezing of dendritic cells, generated from cryopreserved leukaphereses, does not influence their ability to induce antigen-specific immune responses or functionally react to maturation stimuli.
AuthorsLewalle P, Rouas R, Lehmann F, Martiat P
JournalJ Immunol Methods
PubMed ID10854602
'It is of practical clinical importance to be able to reinfuse into patients dendritic cells which have been previously frozen in aliquots. However, there are few studies comparing the function of fresh and frozen dendritic cells. We therefore decided to perform a systematic immunophenotypic and functional comparison of fresh and ... More
IL-12 completely blocks ultraviolet-induced secretion of tumor necrosis factor alpha from cultured skin fibroblasts and keratinocytes.
AuthorsWerth VP, Bashir MM, Zhang W
JournalJ Invest Dermatol
PubMed ID12535207
'Interleukin-12 is an important regulator of other cytokines. Although interleukin-12 is considered to act primarily on lymphocytes, provoking a shift from T helper 2 to T helper 1 cells and an increase in lymphocyte-derived tumor necrosis factor alpha, we hypothesized that interleukin-12 might also affect tumor necrosis factor alpha secretion ... More
Tacrolimus (FK506) treatment of CD34+ hematopoietic progenitor cells promote the development of dendritic cells that drive CD4+ T cells toward Th2 responses.
AuthorsShimizu K, Fujii S, Fujimoto K, Kawa K, Yamada A, Kawano F
JournalJ Leukoc Biol
PubMed ID11073101
'The macrolide lactone, tacrolimus (FK506), is utilized in bone marrow transplantation (BMT) to prevent graft-versus-host disease (GVHD). In the current study, we evaluated the ability of FK506 to modify the function of dendritic cells (DCs) derived from CD34+ hematopoietic progenitor cells (HPCs). Comparable to DCs obtained in the absence of ... More
IL-12 and IL-18 induction and subsequent NKT activation effects of the Japanese botanical medicine Juzentaihoto.
AuthorsFujiki K, Nakamura M, Matsuda T, Isogai M, Ikeda M, Yamamoto Y, Kitamura M, Sazaki N, Yakushiji F, Suzuki S, Tomiyama J, Uchida T, Taniguchi K
JournalInt J Mol Sci
PubMed ID19325795
'In this study, we first measured some cytokine concentrations in the serum of patients treated with Juzentaihoto (JTT). Of the cytokines measured interleukin (IL) -18 was the most prominently up-regulated cytokine in the serum of patients under long term JTT administration. We next evaluated the effects of JTT in mice, ... More
Generation of antigen-specific cytotoxic T lymphocytes using a leukemic plasmacytoid dendritic cell line as antigen presenting cells.
AuthorsYamahira A, Narita M, Nakamura T, Watanabe N, Kaji M, Taniguchi T, Hashimoto S, Furukawa T, Toba K, Aizawa Y, Kuzushima K, Takahashi M
JournalLeuk Res
PubMed ID21216464
'Establishment of a leukemia plasmacytoid dendritic cell line (PMDC05) and intra-lineage transformation from pDCs to mDCs in PMDC05 has been reported. In this paper, we show the applicability of PMDC05 for cellular immunotherapy. By stimulation with LPS, PMDC05 showed enhancement in expression of antigen presentation-associated surface molecules and production of ... More
Altered chemokine levels in individuals at risk of Type 1 diabetes mellitus.
The hypothesis was tested in an exploratory study that individuals at high risk of developing Type 1 diabetes mellitus have altered systemic levels of cytokines and chemokines. ... More
Absence of mucosal inflammation in uncomplicated diverticular disease.
AuthorsElli L, Roncoroni L, Bardella MT, Terrani C, Bonura A, Ciulla M, Marconi S, Piodi L
JournalDig Dis Sci
PubMed ID21221786
Uncomplicated diverticular disease is a common condition in patients older than 50 years. Symptoms are aspecific and overlapping with those of irritable bowel syndrome. Nowadays, patients are often treated with antinflammatory drugs (5-aminosalicilic acid). ... More
Adenoviral interleukin-12 gene transduction into human bronchial epithelial cells: up-regulation of pro-inflammatory cytokines and its prevention by corticosteroids.
AuthorsOgawa H, Nishimura N, Nishioka Y, Azuma M, Yanagawa H, Sone S
JournalClin Exp Allergy
PubMed ID12859448
One of the potential effects of IL-12 is to restore Th1/Th2 balance. Therefore, we investigated the possibility of developing a system for local delivery of IL-12 into the airways by examining protein expression in a human bronchial epithelial cell line (BEAS-2B) after adenoviral IL-12 gene transduction. The effects of dexamethasone ... More
Intranasal interleukin-12 gene therapy enhanced the activity of ifosfamide against osteosarcoma lung metastases.
AuthorsDuan X, Jia SF, Koshkina N, Kleinerman ES
JournalCancer
PubMed ID16453328
Cyclophosphamide (CTX) and ifosfamide (IFX) are alkylating agents used to treat osteosarcoma (OS). It was previously demonstrated that the sensitivity of OS cells to 4-hydroperoxycyclophosphamide (4-HC, the active metabolite of CTX) is augmented by interleukin (IL)-12 in vitro through a mechanism involving the Fas/FasL pathway. The purpose of these studies ... More
GM-CSF promotes differentiation of human dendritic cells and T lymphocytes toward a predominantly type 1 proinflammatory response.
AuthorsEksioglu EA, Mahmood SS, Chang M, Reddy V
JournalExp Hematol
PubMed ID17562355
We recently demonstrated that patients with high levels of circulating dendritic cells (DC) and interleukin (IL)-12 are associated with reduced cancer relapse after hematopoietic stem cell transplantation. Identifying a growth factor that can promote these immune functions may have beneficial anti-tumor effects. We investigated the hypothesis that granulocyte-macrophage colony-stimulating factor ... More
Transformation of dendritic cells from plasmacytoid to myeloid in a leukemic plasmacytoid dendritic cell line (PMDC05).
AuthorsWatanabe N, Narita M, Yamahira A, Nakamura T, Tochiki N, Saitoh A, Kaji M, Hashimoto S, Furukawa T, Toba K, Fuse I, Aizawa Y, Takahashi M
JournalLeuk Res
PubMed ID20338639
We investigated the transformation from pDCs to mDCs in a pDC line (PMDC05) which was established from a patient with pDC leukemia in our laboratory. PMDC05 cells were separated into two fractions according to the expression of BDCA1 and CD123. BDCA1(-)CD123(+) cells were found to be pDC-like cells by their ... More
Mechanism of CD1d-restricted natural killer T cell activation during microbial infection.
AuthorsBrigl M, Bry L, Kent SC, Gumperz JE, Brenner MB
JournalNat Immunol
PubMed ID14578883
CD1d-restricted natural killer T (NKT) cells are important for host defense against a variety of microbial pathogens. How and when these T cells become activated physiologically during infection remains unknown. Our data support a model in which NKT cells use a unique activation mechanism not requiring their recognition of microbial ... More
OM197-MP-AC induces the maturation of human dendritic cells and promotes a primary T cell response.
AuthorsByl B, Libin M, Bauer J, Martin OR, De Wit D, Davies G, Goldman M, Willems F
JournalInt Immunopharmacol
PubMed ID12639819
Dendritic cell (DC) maturation is critical for the induction of antigen-specific T lymphocyte responses and may be essential for the development of human vaccines relying on T cell immunity. We investigated the effects on human DC of OM-197, a synthetic pseudodipeptide derived from amino acids, linked to three fatty acid ... More
Potent stimuli combined with lipopolysaccaride and IFNgamma may improve immunotherapy against HCC by increasing the maturation and subsequent immune response of the dendritic cells.
AuthorsIwashita Y, Goto S, Tahara K, Kawano K, Chen CL, Kitano S
JournalCancer Biother Radiopharm
PubMed ID12667303
Dendritic cells (DCs)-based immunotherapy is a new strategy for cancer treatment and has been used in some clinical trials against cancer, including melanoma, and has shown promising results. However, the conventional protocol of DC immunotherapy may not be effective for hepatocellular carcinoma (HCC) because of impaired DC maturation in HCC ... More
Chemokine/cytokine production by mononuclear cells from human lymphoid tissues and their modulation by Mycobacterium tuberculosis antigens.
AuthorsArias MA, Pantoja AE, Jaramillo G, López YP, Mejía N, Mejía C, Paris SC, Shattock RJ, Griffin GE, García LF
JournalFEMS Immunol Med Microbiol
PubMed ID17328761
The majority of knowledge about the role of cytokines and chemokines in controlling Mycobacterium tuberculosis infection mainly derives from animal models. In humans, this knowledge is still mainly limited to the blood compartment or accessible lymphoid organs, such as tonsils. Here, we studied cytokine and chemokine production and their modulation ... More
IL-12 secreting dendritic cells are required for optimum activation of human secondary lymphoid tissue T cells.
AuthorsRouard H, Marquet J, Léon A, Maison P, Haioun C, Copie-Bergman C, Plonquet A, Farcet JP, Delfau-Larue MH
JournalJ Immunother
PubMed ID12142555
Successful immunization requires that mature dendritic cells (mDCs) prime T cells in secondary lymphoid tissue (LT). Previously, the authors have shown that LT T cell activation has an increased costimulatory threshold for a proliferative response as compared with peripheral blood (PB) T cells. Therefore, to optimize mDC immunogenicity, DC maturation ... More
Differential uptake and cross-presentation of human papillomavirus virus-like particles by dendritic cells and Langerhans cells.
AuthorsFausch SC, Da Silva DM, Kast WM
JournalCancer Res
PubMed ID12839929
A causal link between cervical cancer and high-risk human papillomaviruses has been established. The virus infects basal cells of the mucosa, where Langerhans cells are the resident antigen-presenting cells. Langerhans cells and dendritic cells, which are targeted by vaccination, internalize similar amounts of human papillomavirus virus-like particles (VLPs), albeit through ... More
Helper function of memory CD8+ T cells: heterologous CD8+ T cells support the induction of therapeutic cancer immunity.
In contrast to the well-established efficacy of preventive vaccines, the effectiveness of therapeutic vaccines remains limited. To develop effective vaccination regimens against cancer, we have analyzed the effect of effector and memory CD8+ T cells on the ability of dendritic cells to mediate the immunologic and antitumor effects of vaccination. ... More
Regulatory balance between the immune response of tumor antigen-specific T-cell receptor gene-transduced CD8 T cells and the suppressive effects of tolerogenic dendritic cells.
AuthorsFujii S, Nishimura MI, Lotze MT
JournalCancer Sci
PubMed ID16367910
Tumor immune responses, including some immunotherapy strategies, can fail because of a number of reasons, such as poor tumor cell immunogenicity or local suppressive cytokine release by dendritic cells (DC) at tumor sites. The retroviral transfer of T-cell receptor (TCR) genes encoding tumor-specific receptors into T cells is a fascinating ... More
Critical role of protein kinase C epsilon for lipopolysaccharide-induced IL-12 synthesis in monocyte-derived dendritic cells.
AuthorsAksoy E, Amraoui Z, Goriely S, Goldman M, Willems F
JournalEur J Immunol
PubMed ID12385023
In the present study we have investigated the potential involvement of protein kinase C (PKC) in the maturation of human dendritic cells (DC) by bacterial lipopolysaccharide (LPS). LPS stimulation of DC derived from human monocytes resulted in PKC phosphorylation. Inhibition of PKC activation using bisindolylmaleimide (Bis), a pan-PKC inhibitor, was ... More
Quantitative analysis of chemokine expression by dendritic cell subsets in vitro and in vivo.
Upon maturation, dendritic cells (DCs) have to adjust their chemokine expression to sequentially attract different leukocyte subsets. We used real-time quantitative polymerase chain reaction analysis to study in detail the expression of 12 chemokines involved in the recruitment of leukocytes into and inside secondary lymphoid organs, by DCs in distinct ... More
Functional defects of dendritic cells in patients with CD40 deficiency.
AuthorsFontana S, Moratto D, Mangal S, De Francesco M, Vermi W, Ferrari S, Facchetti F, Kutukculer N, Fiorini C, Duse M, Das PK, Notarangelo LD, Plebani A, Badolato R
JournalBlood
PubMed ID12893749
We have recently identified 2 patients with a rare autosomal recessive form of hyper IgM disease, known as HIGM3, caused by mutations in the CD40 gene. These patients had opportunistic infections observed on X-linked hyper IgM syndrome (HIGM), suggesting that the CD40-CD40 ligand interaction is important for promoting T-cell-mediated immunity. ... More
High mobility group B1 protein suppresses the human plasmacytoid dendritic cell response to TLR9 agonists.
AuthorsPopovic PJ, DeMarco R, Lotze MT, Winikoff SE, Bartlett DL, Krieg AM, Guo ZS, Brown CK, Tracey KJ, Zeh HJ
JournalJ Immunol
PubMed ID17142771
Plasmacytoid dendritic cells (PDC) are innate immune effector cells that are recruited to sites of chronic inflammation, where they modify the quality and nature of the adaptive immune response. PDCs modulate adaptive immunity in response to signals delivered within the local inflammatory milieu by pathogen- or damage-associated molecular pattern, molecules, ... More
Impaired function of dendritic cells circulating in patients infected with hepatitis C virus who have persistently normal alanine aminotransferase levels.
AuthorsKanto T, Inoue M, Miyazaki M, Itose I, Miyatake H, Sakakibara M, Yakushijin T, Kaimori A, Oki C, Hiramatsu N, Kasahara A, Hayashi N
JournalIntervirology
PubMed ID16166790
Hepatitis C virus (HCV) induces chronic liver disease in hosts which can eventually progresses to liver cirrhosis and hepatocellular carcinoma. However, progression of liver disease is slower in patients with persistently normal levels of alanine aminotransferase (ALT) than in those with active hepatitis. Although distinct immune responses against HCV have ... More
Tumor-derived MUC1 mucins interact with differentiating monocytes and induce IL-10highIL-12low regulatory dendritic cell.
AuthorsMonti P, Leone BE, Zerbi A, Balzano G, Cainarca S, Sordi V, Pontillo M, Mercalli A, Di Carlo V, Allavena P, Piemonti L
JournalJ Immunol
PubMed ID15187110
Dendritic cells (DC) initiate immunity by the activation of naive T cells and control immunity through their ability to induce unresponsiveness of lymphocytes by mechanisms that include deletion and induction of regulatory cells. An inadequate presentation to T cells by tumor-induced "regulatory" DC, among several mechanisms, can explain tolerance to ... More
Type 1-polarized dendritic cells loaded with autologous tumor are a potent immunogen against chronic lymphocytic leukemia.
AuthorsLee JJ, Foon KA, Mailliard RB, Muthuswamy R, Kalinski P
JournalJ Leukoc Biol
PubMed ID18426971
Induction of active tumor-specific immunity in patients with chronic lymphocytic leukemia (CLL) and other hematologic malignancies is compromised by the deficit of endogenous dendritic cells (DCs). In attempt to develop improved vaccination strategies for patients with CLL and other tumors with poorly identified rejection antigens, we tested the ability of ... More
P-glycoprotein functions as a differentiation switch in antigen presenting cell maturation.
AuthorsPendse SS, Behjati S, Schatton T, Izawa A, Sayegh MH, Frank MH
JournalAm J Transplant
PubMed ID17083370
P-glycoprotein (P-gp) expressed on human antigen presenting cells (APC) regulates alloantigen-dependent T-cell activation, but the associated mechanisms are not well understood. Here we demonstrate that P-gp functions in IL-12-dependent monocyte differentiation into dendritic cell (DC) lineages during APC maturation, thereby regulating the capacity of myeloid-derived APCs to elicit alloimmune Th1 ... More
OxLDL immune complexes activate complement and induce cytokine production by MonoMac 6 cells and human macrophages.
AuthorsSaad AF, Virella G, Chassereau C, Boackle RJ, Lopes-Virella MF
JournalJ Lipid Res
PubMed ID16804192
Oxidized low density lipoprotein (OxLDL) is immunogenic and induces autoimmune responses in humans. OxLDL antibodies are predominantly of the proinflammatory IgG1 and IgG3 isotypes. We tested the capacity of immune complexes prepared with copper-oxidized human LDL and affinity chromatography-purified human OxLDL antibodies [OxLDL-immune complexes (ICs)] to activate complement and to ... More
Heterologous papillomavirus virus-like particles and human papillomavirus virus-like particle immune complexes activate human Langerhans cells.
AuthorsFausch SC, Da Silva DM, Kast WM
JournalVaccine
PubMed ID15705478
Chimeric human papillomavirus virus-like particles (HPV cVLP) are currently being explored as a therapeutic vaccination strategy against cervical cancer. HPV cVLP are being explored as a result of their interaction with and activation of dendritic cells, a potent antigen-presenting cell. However Langerhans cells, another type of antigen-presenting cell, can interact ... More
Reduced number and function of peripheral dendritic cells in coeliac disease.
AuthorsCiccocioppo R, Ricci G, Rovati B, Pesce I, Mazzocchi S, Piancatelli D, Cagnoni A, Millimaggi D, Danova M, Corazza GR
JournalClin Exp Immunol
PubMed ID17581262
Dendritic cells (DC) play a pivotal role in shaping the immune response in both physiological and pathological conditions. In peripheral blood at least two subsets, the myeloid and plasmacytoid, have been described as having different T stimulatory functions and a variable degree of maturation. Certainly, antigen presentation plays a crucial ... More
Quick generation of fully mature dendritic cells from monocytes with OK432, low-dose prostanoid, and interferon-alpha as potent immune enhancers.
AuthorsSakakibara M, Kanto T, Inoue M, Kaimori A, Yakushijin T, Miyatake H, Itose I, Miyazaki M, Kuzushita N, Hiramatsu N, Takehara T, Kasahara A, Hayashi N
JournalJ Immunother
PubMed ID16365602
Dendritic cells (DCs) are one of the promising tools for enhancing antigen-specific immune responses in clinical settings. Many studies have been performed thus far to verify the efficacy of the DC vaccine in cancer patients; however, the responses have not always been satisfactory, partly because of DC incompetence. To obtain ... More
FcgammaR cross-linking mediates NF-kappaB activation, reduced antigen presentation capacity, and decreased IL-12 production in monocytes without modulation of myeloid dendritic cell development.
AuthorsDrechsler Y, Chavan S, Catalano D, Mandrekar P, Szabo G
JournalJ Leukoc Biol
PubMed ID12377934
Stimulation of monocytes (MO) through receptors for the Fc region of immunoglobulin G (FcgammaR) activates a variety of responses, including phagocytosis, antibody (Ab)-dependent cellular cytotoxicity, and production of cytokines. We previously reported that the MO subpopulation that expresses FcgammaR in high density produces high levels of tumor necrosis factor alpha ... More
Effect of CXC chemokine platelet factor 4 on differentiation and function of monocyte-derived dendritic cells.
AuthorsXia CQ, Kao KJ
JournalInt Immunol
PubMed ID12882838
Platelet factor 4 (PF4) is a CXC chemokine secreted by activated platelets. PF4 has been shown to promote monocyte survival and induce the differentiation of monocytes into macrophages. However, the effect of PF4 on differentiation of monocytes into dendritic cells (DC) has yet to be determined. As reported previously, monocytes ... More