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引用和文献 (11)
Invitrogen™
XCell SureLock™ 小型槽
The XCell SureLock Mini-Cell is a vertical mini-protein gel electrophoresis system compatible with all Invitrogen mini precast gels and Surecast了解更多信息
| 货号 | 数量 |
|---|---|
| EI0001 | 1 unit |
货号 EI0001
价格（CNY)
6,947.00
Each
数量:
1 unit
价格（CNY)
6,947.00
Each
The XCell SureLock Mini-Cell is a vertical mini-protein gel electrophoresis system compatible with all Invitrogen mini precast gels and Surecast handcast gels. The Mini-Cell is simple, sturdy, and easy to use. Rather than using clamps, the system incorporates the use of a gel tension wedge to make setup quick and convenient.
How the system works
The XCell SureLock Mini-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even, horizontal force is generated. This seals the gel/buffer core assembly firmly into position in the lower buffer chamber. The positive locking action of the gel tension wedge ensures a trouble-free, leak-free gel run every time.
The XCell SureLock Mini-Cell can be used for wet transfers using the XCell II Blot Module. The XCell II Blot Module is used in place of the gel/buffer core assembly. It requires less than 200 mL of transfer buffer for western, southern, and northern transfers. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9 cm x 9 cm.
How the system works
The XCell SureLock Mini-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even, horizontal force is generated. This seals the gel/buffer core assembly firmly into position in the lower buffer chamber. The positive locking action of the gel tension wedge ensures a trouble-free, leak-free gel run every time.
The XCell SureLock Mini-Cell can be used for wet transfers using the XCell II Blot Module. The XCell II Blot Module is used in place of the gel/buffer core assembly. It requires less than 200 mL of transfer buffer for western, southern, and northern transfers. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9 cm x 9 cm.
仅供科研使用。不可用于诊断程序。
规格
数量1 unit
运输条件室温
容量多达 2 块小型凝胶
适用于（设备）XCell SureLock™ 小型
凝胶兼容性Novex™ 小型胶、NuPAGE™ 凝胶
凝胶尺寸微型 (8 cm x 8 cm)
最大电压500 V dc
产品线XCell SureLock
类型Mini-Cell 小型垂直电泳系统
Unit SizeEach
内容与储存
在室温下储存。自购买之日起享受一年质保。
常见问题解答 (FAQ)
我正在以恒定电压进行Tris-甘氨酸凝胶转印，但电流读数高于预期起始电流。可能因为什么？
我将蛋白质置于Tris-甘氨酸凝胶上在非变性条件下进行电泳。蛋白质的pI高于Tris-甘氨酸转膜缓冲液的pH。你们建议如何对蛋白质进行转印？
我在对NuPAGE凝胶上的大分子蛋白质进行转印时遇到问题。你们有何建议？
转印后，为什么膜上出现空白的点？
我在蛋白质转印后发现膜上的条带呈扩散状和旋涡状。可能原因是什么？
引用和文献 (11)
引用和文献
Abstract
Enzymatic hydrolysis of pyridoxine-5'-beta-D-glucoside is catalyzed by intestinal lactase-phlorizin hydrolase.
Journal:J Biol Chem
PubMed ID:12023280
'An obligatory step in the mammalian nutritional utilization of pyridoxine-5''-beta-D-glucoside (PNG) is the intestinal hydrolysis of its beta-glucosidic bond that releases pyridoxine (PN). This laboratory previously reported the purification and partial characterization of a novel cytosolic enzyme, designated PNG hydrolase, which hydrolyzed PNG. An investigation of the subcellular distribution of
Definition of genetically distinct attenuation mechanisms in naturally virulence-attenuated Listeria monocytogenes by comparative cell culture and molecular characterization.
Journal:Appl Environ Microbiol
PubMed ID:16000803
'Listeria monocytogenes is a foodborne pathogen able to cause serious disease in humans and animals. Not all isolates are equally pathogenic, however, and several isolates have been characterized as naturally virulence attenuated. We sought to identify the genetic basis of natural virulence attenuation using cell culture assays and molecular techniques.
Combined effect of epinephrine and exercise on calpain/calpastatin and cathepsin B and L activity in porcine longissimus muscle.
Journal:J Anim Sci
PubMed ID:10492449
The objective of the study was to improve the understanding of the relationship between the effect of epinephrine plus exercise and meat tenderness. The calpain, calpastatin, and cathepsin B + L activities and postmortem proteolysis in porcine longissimus muscle were studied. The muscle glycogen stores were depleted in five pigs
Apolipoprotein A-I is a selective target for myeloperoxidase-catalyzed oxidation and functional impairment in subjects with cardiovascular disease.
Journal:J Clin Invest
PubMed ID:15314690
In recent studies we demonstrated that systemic levels of protein-bound nitrotyrosine (NO(2)Tyr) and myeloperoxidase (MPO), a protein that catalyzes generation of nitrating oxidants, serve as independent predictors of atherosclerotic risk, burden, and incident cardiac events. We now show both that apolipoprotein A-I (apoA-I), the primary protein constituent of HDL, is
Regulation of the mitogen-activated protein kinase p44 ERK activity during anoxia/recovery in rainbow trout hypodermal fibroblasts.
Journal:J Exp Biol
PubMed ID:16621957
It is well known from various mammalian cells that anoxia has a major impact on the mitogen-activated protein kinase ERK, but a possible similar effect in fish cells has not been investigated. Here we characterise a p44ERK-like protein in the rainbow trout cell line RTHDF and study the effect of