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查看更多产品信息 PowerEase® 500 Power Supply (100/120 VAC, 50/60 Hz) - FAQs (EI8600)
18 个常见问题解答
使用PowerEase 500电源时，可同时进行电泳的Tris-甘氨酸凝胶或NuPAGE凝胶数量如下：
Tris-甘氨酸凝胶：10块凝胶，即5块XCell SureLock小型凝胶
NuPAGE Bis-Tris凝胶：2块凝胶，即1块XCell SureLock小型凝胶
NuPAGE Tris-Acetate凝胶：6块凝胶，即3块XCell SureLock小型凝胶
注意：PowerEase 500电源已于2013年12月31日起停产，我们的新型替代品为PowerEase 300W电源和PowerEase 90W电源。
除了偶尔擦掉仪器上逐渐积累的盐类残留物以外，PowerEase 500电源不需要其他定期维护。
注意：PowerEase 500电源已于2013年12月31日起停产，我们的新型替代品为PowerEase 300W电源和PowerEase 90W电源。
PowerEase 500电源可在恒定电压、恒定电流和恒定功率多种条件下运行。默认设置为恒定电压。我们推荐在恒定电压下进行预制胶电泳，原因如下：
•预制胶为连续性凝胶
•对于大部分电泳方法，电阻在整个电泳过程中不断增加。在恒定电压下，电流和功率在整个电泳过程中不断降低，形成自然的安全界限
•即使同时进行电泳的凝胶数量和厚度不同，也可使用相同的电压设置。
如果您想在恒定功率下使用PowerEase 500电源进行凝胶电泳，可先设置电压极限（等于或稍高于电泳过程中可达到的最大预期电压），再将功率设置到所需水平。如果在不限制电压的情况下出现局部故障，如连接不良，则非常高的局部电阻可能导致局部过热，并损害电泳装置和/或凝胶。
注意：PowerEase 500电源已于2013年12月31日起停产，我们的新型替代品为PowerEase 300W电源和PowerEase 90W电源。
PowerEase 500电源可在恒定电压、恒定电流和恒定功率多种条件下运行。默认设置为恒定电压。我们推荐在恒定电压下进行预制胶电泳，原因如下：
•预制胶为连续性凝胶
•对于大部分电泳方法，电阻在整个电泳过程中不断增加。在恒定电压下，电流和功率在整个电泳过程中不断降低，形成自然的安全界限；而且，即使同时进行电泳的凝胶数量和厚度不同，也可使用相同的电压设置。
如果您想在恒定电流下使用PowerEase 500电源进行凝胶电泳，可先设置电压极限（等于或稍高于电泳过程中可达到的最大预期电压），再将电流设置到所需水平。用这种方法，设备会努力达到设定的电压。但是，当达到阈值电流时，电流将不再变化，电泳会转换为恒定电流（限制因素）模式。如果在不限制电压的情况下出现局部故障，如连接不良，则非常高的局部电阻可能导致电压升高并达到电源的最大容量。这可能引起局部过热，并损害电泳装置和/或凝胶，或产生不安全因素。
注意：PowerEase 500电源已于2013年12月31日起停产，我们的新型替代品为PowerEase 300W电源和PowerEase 90W电源。
可以。但是，应记住液晶屏幕在冷藏室内会逐渐消失。但是，绿色和红色指示灯表明PowerEase仍可运行。回到室温后，屏幕将恢复。
注意：PowerEase 500电源已于2013年12月31日起停产，我们的新型替代品为PowerEase 300W电源和PowerEase 90W电源。
PowerEase 500电源已停产。我们的新型替代品为PowerEase 300W电源和PowerEase 90W电源。
在NativePAGE电泳期间，电流下降至低于1 mA很常见。PowerEase 300W电源会将此记录为“空载”错误并自动关闭，导致电泳停止。由于无法禁用或关闭PowerEase 300W电源的“负载检测”功能，我们建议不要将其用于Native-PAGE电泳。
The most common power supplies from Thermo Fisher Scientific, Bio-Rad, and Hoefer are compatible. Also, power supply adapters are available for power supplies not designed for use with covered or non-retractable power leads. Thermo Fisher Owl branded gel tanks are not compatible
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The NativePAGE Invitrogen Bis-Tris Gel System is a pre-cast polyacrylamide mini gel system that provides a sensitive and high-resolution method for analyzing native membrane protein complexes, native soluble proteins, molecular mass estimations, and assessing purity of native proteins. It is based on the blue native polyacrylamide gel electrophoresis technique (BN PAGE) developed by Schagger and von Jagow.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Specifications for Temperature Monitoring Probe:
1) Resistance: 10 kOhm @ 25°C (44036).
2) Interchangeability: ±0.1°C from 0°C to 70°C.
3) Electrical isolation: Greater than 100 mOhm with 500 VDC applied between internal conductors and conductive beads surrounding thermistor bead.
We recommend rinsing the membrane briefly in water, air drying and store it at room temperature in a ziplock bag. Do not place nitrocellulose in the freezer because it will shatter. Unlike PVDF, nitrocellulose membranes should never be pre-wetted in alcohol as it will cause them to shrivel.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Linear mode is a feature of the PowerEase 500 Power Supply that has not been described in the manual. The linear mode can be activated from the 'Edit' screen (where you would change settings for time, voltage, power, current). While on the “Edit” screen, press the left and right arrows simultaneously. This will activate another screen with a three choice menu where the third choice is linear mode. Move the cursor down to 'Linear mode' and turn on Linear mode by using the up/down arrows.
Note: The PowerEase 500 Power Supply has been discontinued as of December 31, 2013 and our newer alternatives are the PowerEase 300W Power Supply and PowerEase 90W Power Supply.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Using the PowerEase 500 Power Supply, the number of Tris-Glycine or NuPAGE gels that can be run simultaneously is shown below:
- Tris-Glycine gels: 10 gels i.e., 5 XCell SureLock Mini-Cells
- NuPAGE Bis-Tris gels: 2 gels i.e., 1 XCell SureLock Mini-Cell
- NuPAGE Tris-Acetate gels: 6 gels i.e., 3 XCell SureLock Mini-Cells
Note: The PowerEase 500 Power Supply has been discontinued as of December 31, 2013 and our newer alternatives are the PowerEase 300W Power Supply and PowerEase 90W Power Supply.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Other than an occasional wipe-down of the instrument to remove salt deposits, which may have built up over time, the PowerEase 500 Power Supply does not require a periodic maintenance program.
Note: The PowerEase 500 Power Supply has been discontinued as of December 31, 2013 and our newer alternatives are the PowerEase 300W Power Supply and PowerEase 90W Power Supply.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The PowerEase 500 Power Supply can be run under a variety of conditions including constant voltage, constant current, and constant power. The default setting is for constant voltage. We recommend running our precast gels at constant voltage for the following reasons:
- They are continuous gels
- For most electrophoresis methods, resistance increases throughout the run. When run at constant voltage, current and power decrease throughout the run, providing a natural safety margin
- The same voltage setting can be used regardless of the number, or thickness of gels being run
If you want to use the PowerEase 500 Power Supply to run a gel at constant power, this can be accomplished by setting a voltage limit (at or slightly above the maximum voltage expected to be reached during the run) and then setting the power to the desired level. If no voltage limit is set and a local fault condition occurs, such as a poor connection, very high local resistance may cause a high proportion of the total heat to be generated over a small distance, damaging the electrophoresis unit and/or gel(s). Note: The PowerEase 500 Power Supply has been discontinued as of December 31, 2013 and our newer alternatives are the PowerEase 300W Power Supply and PowerEase 90W Power Supply.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The PowerEase 500 Power Supply can be operated under a variety of conditions including constant voltage, constant current and constant power. The default setting is for constant voltage. We recommend running our precast gels at constant voltage for the following reasons:
- They are continuous gels
- For most electrophoresis methods, resistance increases throughout the run. When run at constant voltage, current and power decrease throughout the run, providing a natural safety margin
- The same voltage setting can be used regardless of the number, or thickness of gels being run
If you would like to use the PowerEase 500 Power Supply to run a gel at constant current, this can be accomplished by setting a voltage limit (at or slightly above the maximum voltage expected to be reached during the run) and then setting the current to the desired level. In this way, the device will try to reach the set voltage. However, when it reaches the threshold current, the current will plateau and the run will switch to constant current (the limiting factor). If no voltage limit is set and a local fault condition occurs, such as a poor connection, very high local resistance may cause the voltage to increase to the maximum of the power supply. This can lead to local overheating and damage to the electrophoresis unit and or gel(s) or create unsafe conditions. Note: The PowerEase 500 Power Supply has been discontinued as of December 31, 2013 and our newer alternatives are the PowerEase 300W Power Supply and PowerEase 90W Power Supply.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Yes, however, keep in mind that the LCD screen will fade away in the cold room. The PowerEase would still be working and this will be indicated by the green and red light. The screen should come back on when returned to room temp.
Note: The PowerEase 500 Power Supply has been discontinued as of December 31, 2013 and our newer alternatives are the PowerEase 300W Power Supply and PowerEase 90W Power Supply.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The PowerEase 500 Power Supply has been discontinued. Our newer alternatives are the PowerEase 300W Power Supply and PowerEase 90W Power Supply.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.