T4 RNA 连接酶 (10 U/μL)

Name: T4 RNA 连接酶 (10 U/μL)
SKU: EL0021
Price: 643.00 CNY

Thermo Scientific T4 RNA 连接酶催化寡核苷酸、单链 RNA 和 DNA 分子间和分子内了解更多信息

货号 EL0021

价格（CNY)

643.00

飞享价

Ends: 31-Dec-2025

917.00

共减 274.00 (30%)

Each

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请求批量或定制报价

价格（CNY)

643.00

飞享价

Ends: 31-Dec-2025

917.00

共减 274.00 (30%)

Each

添加至购物车

Thermo Scientific T4 RNA 连接酶催化寡核苷酸、单链 RNA 和 DNA 分子间和分子内 5'-磷酸基团和 3'-羟基末端间磷酸二酯键的 ATP 依赖性形成。

最少底物：分子间反应中为一个核苷 3',5'-磷酸氢盐，分子内反应中为 8碱基寡核苷酸。

应用：

•RNA 3'-末端标记（使用胞苷 3',5'-二[α-³²P]磷酸）
•RNA 与 RNA 连接
•寡核糖核苷酸和寡脱氧核糖核苷酸的合成
•tRNA 特异性修饰
•寡脱氧核苷酸连接至单链 cDNA 以实现 5' RACE（cDNA 末端快速扩增）
• PCR 复合引物的位点特异性生成

注

反应混合物中 BSA 的推荐浓度为 0.1 mg/mL。

For Research Use Only. Not for use in diagnostic procedures.

规格

兼容缓冲液10X 反应缓冲液

产品类型T4 RNA 连接酶

数量1000 U

最大浓度10 U/μL

酶T4 RNA 连接酶

Unit SizeEach

常见问题解答 (FAQ)

What are some of the problems associated with sticky-end cloning?

The amplified DNA needs to be purified from the PCR mixture components prior to cloning. The dNTPs carried over from the PCR are competitive inhibitors for ATP in the ligation reaction.

If during synthesis of the PCR primers their chemical integrity has been compromised by either a base substitution or modification, the enzyme recognition site may in actuality not exist. If this is the case, PCR products will be resistant to digestion with restriction enzymes. It may be necessary to use a higher concentration of the restriction enzyme and to incubate at the appropriate temperature overnight to ensure cutting.