Interleukin-6 deficiency increases inflammatory bone destruction.
AuthorsBalto K, Sasaki H, Stashenko P,
JournalInfect Immun
PubMed ID11159963
'Periapical bone destruction occurs as a consequence of pulpal infection. In previous studies, we showed that interleukin-1 (IL-1) is the primary stimulator of bone destruction in this model. IL-6 is a pleiotropic cytokine that is induced in these infections and has both pro- and anti-inflammatory activities. In the present study, ... More
Changes in serum levels of cytokines in mice injected with an immunostimulator C3bgp isolated from Cuscuta europea.
AuthorsStanilova S, Dobreva Z, Zhelev Z
JournalInt Immunopharmacol
PubMed ID11515822
'The effect of C3 binding glycoprotein (C3bgp), isolated from Cuscuta europea seeds on induction of in vivo cytokine synthesis was investigated. Different groups of mice were stimulated with 30 microg C3bgp per mouse, injected intraperitoneally. The quantitative determination of IL-1alpha, IL-2, IL-4, IL-6, IL-10, TNF-alpha and IFN-gamma was performed in ... More
Nanoemulsions of an anti-oxidant synergy formulation containing gamma tocopherol have enhanced bioavailability and anti-inflammatory properties.
AuthorsKuo F, Subramanian B, Kotyla T, Wilson TA, Yoganathan S, Nicolosi RJ
JournalInt J Pharm
PubMed ID18718513
'The present study investigated whether MicroFluidizer Processor-based nanoemulsions of an antioxidant synergy formulation (ASF), containing delta, alpha and gamma tocopherol influenced inflammation and bioavailability in CD-1 mice. Croton oil was applied to all animals'' right ear lobe to induce inflammation. Auricular thickness was measured after 2 and 6h after the ... More
The osterix transcription factor down-regulates interleukin-1 alpha expression in mouse osteosarcoma cells.
AuthorsCao Y, Jia SF, Chakravarty G, de Crombrugghe B, Kleinerman ES
JournalMol Cancer Res
PubMed ID18234967
'K7M2 mouse osteosarcoma cells form lytic tumors and are deficient in osterix (Osx), a zinc finger-containing transcription factor required for osteoblast differentiation and bone formation. Our previous studies showed that replacement of Osx suppresses lytic bone destruction. Cytokines, including interleukin (IL)-1alpha, IL-6, IL-11, and prostaglandin E2, have been shown to ... More
Autocrine action of IL-10 suppresses proinflammatory mediators and inflammation in the HSV-1-infected cornea.
AuthorsYan XT, Zhuang M, Oakes JE, Lausch RN
JournalJ Leukoc Biol
PubMed ID11200059
'We investigated whether IL-10 produced endogenously would influence the development of HSV-1-induced acute corneal disease. Murine corneal epithelial cells and fibroblasts cultured in vitro expressed IL-10 mRNA and protein constitutively and also IL-10 receptors. Inclusion of IL-10 neutralizing antibody in the culture medium significantly (p<0.05) enhanced TNF-alpha-induced IL-6 and MIP-2 ... More
Time course of acute-phase response induced by Tityus serrulatus venom and TsTX-I in mice.
'Animal venom can induce systemic alterations similar to those observed in acute-phase inflammatory response. In the present study, we report the systemic (circulatory) and local (peritoneal cavity) effects induced by Tityus serrulatus venom and its major toxin TsTX-I (Ts1) in mice over various time periods. Both the venom and TsTX-I ... More
Inflammatory skin disease in K14/p40 transgenic mice: evidence for interleukin-12-like activities of p40.
'The proinflammatory cytokine interleukin-12, a p35/p40 heterodimer, is produced by resident cells in skin and has been implicated as a pathogenetic factor in T-cell-mediated skin diseases. Secretion of heterodimeric interleukin-12 is always accompanied by production of p40 monomer and p40/p40 homodimer. To investigate the possible in vivo role of p40 ... More
Epidermal interleukin-1 alpha generation is amplified at low humidity: implications for the pathogenesis of inflammatory dermatoses.
AuthorsAshida Y, Ogo M, Denda M
JournalBr J Dermatol
PubMed ID11251553
We have previously reported that low humidity amplifies the hyperproliferative and inflammatory response to barrier disruption. Other reports suggest that epidermal interleukin (IL)-1 alpha is stimulated by various factors related to epidermal inflammation and that it may induce other proinflammatory molecules. ... More
Toll-like receptor 4-deficient mice have reduced bone destruction following mixed anaerobic infection.
AuthorsHou L, Sasaki H, Stashenko P,
JournalInfect Immun
PubMed ID10899873
C3H/HeJ mice have an impaired ability to respond to lipopolysaccharide (LPS) due to a mutation in the gene that encodes Toll-like receptor 4 (TLR4). The effect of TLR4 deficiency on host responses to endodontic infections is unknown. In the present study, we compared periapical bone destruction, sepsis, and inflammatory cytokine ... More
Selective stimulation of G-CSF gene expression in macrophages by a stimulatory monoclonal antibody as detected by a luciferase reporter gene assay.
AuthorsAoki Y, Sha S, Mukai H, Nishi Y
JournalJ Leukoc Biol
PubMed ID11073117
We have identified a stimulatory monoclonal antibody (mAb) from autoimmune mice that selectively stimulates granulocyte colony-stimulating factor (G-CSF) gene expression in a mouse macrophage cell line. The induction was observed not only in the cell line, but also in normal peritoneal macrophages. This mAb bound to the monocyte/macrophage cell lines ... More
Deregulation of peripheral B-cell development in enhanced severity of collagen-induced arthritis in FcgammaRIIB-deficient mice.
AuthorsNakamura A, Nukiwa T, Takai T
JournalJ Autoimmun
PubMed ID12753808
Accumulating evidence indicates that the type IIB Fc receptor for IgG (FcgammaRIIB) plays a pivotal role in maintaining peripheral tolerance by suppressing excessive humoral and cellular immune responses. However, little is known about the mechanism by which the autoreactive B cells develop in the periphery in FcgammaRIIB-deficient mice. To clarify ... More
Cytokines IL-1 alpha, IL-6, and GM-CSF constitutively secreted by oral squamous carcinoma induce down-regulation of CD80 costimulatory molecule expression: restoration by interferon gamma.
AuthorsThomas GR, Chen Z, Leukinova E, Van Waes C, Wen J
JournalCancer Immunol Immunother
PubMed ID14551747
We previously characterized the expression of CD80 in different murine head and neck squamous cell carcinoma (HNSCC) clones derived following tumor progression in the absence of T cell-mediated immunity in severe combined immunodeficient (SCID) mice. We found that HNSCCs that did not express CD80 grew as progressors, while those that ... More
Role for macrophage inflammatory protein 2 (MIP-2), MIP-1alpha, and interleukin-1alpha in the delayed-type hypersensitivity response to viral antigen.
AuthorsTumpey TM, Fenton R, Molesworth-Kenyon S, Oakes JE, Lausch RN
JournalJ Virol
PubMed ID12134010
BALB/c mice sensitized to herpes simplex virus type 1 (HSV-1) develop a vigorous delayed-type hypersensitivity (DTH) response upon intradermal virus antigen challenge. Although CD4(+) T cells are a key mediator of this response, neutrophils are the most abundant cells at the antigen challenge site both initially and at the peak ... More
Mutual augmentation of the induction of the histamine-forming enzyme, histidine decarboxylase, between alendronate and immuno-stimulants (IL-1, TNF, and LPS), and its prevention by clodronate.
AuthorsDeng X, Yu Z, Funayama H, Shoji N, Sasano T, Iwakura Y, Sugawara S, Endo Y
JournalToxicol Appl Pharmacol
PubMed ID16203021
Nitrogen-containing bisphosphonates (N-BPs), powerful anti-bone-resorptive drugs, have inflammatory side effects, while histamine is not only an inflammatory mediator, but also an immuno-modifier. In murine models, a single intraperitoneal injection of an N-BP induces various inflammatory reactions, including the induction of the histamine-forming enzyme histidine decarboxylase (HDC) in tissues important in ... More
CX-659S, a diaminouracil derivative, indirectly inhibits the function of Langerhans cells by blocking the MEK1/2-Erk1/2 pathway in keratinocytes.
AuthorsUchi H, Koga T, Urabe K, Moroi Y, Furue M
JournalJ Invest Dermatol
PubMed ID12787125
Keratinocytes are an important component of the skin immune system, and keratinocyte-derived cytokines control the function of Langerhans cells. We previously showed that CX-659S, a novel diaminouracil derivative, had an inhibitory effect on hapten-induced contact hypersensitivity reaction in mice. In this study, we investigated the mechanism by which CX-659S elicits ... More