Antibody-mediated protection in murine Cryptococcus neoformans infection is associated with pleotrophic effects on cytokine and leukocyte responses.
AuthorsFeldmesser M, Mednick A, Casadevall A
JournalInfect Immun
PubMed ID11854246
Cryptococcus neoformans, an encapsulated yeast, is a common cause of life-threatening meningoencephalitis in immunosuppressed patients. We previously observed that administration of a monoclonal antibody (MAb) to the capsular polysaccharide to mice with pulmonary infection prolonged survival and enhanced granulomatous inflammation without reducing lung CFU. To understand the mechanism of MAb ... More
Changes in serum levels of cytokines in mice injected with an immunostimulator C3bgp isolated from Cuscuta europea.
AuthorsStanilova S, Dobreva Z, Zhelev Z
JournalInt Immunopharmacol
PubMed ID11515822
'The effect of C3 binding glycoprotein (C3bgp), isolated from Cuscuta europea seeds on induction of in vivo cytokine synthesis was investigated. Different groups of mice were stimulated with 30 microg C3bgp per mouse, injected intraperitoneally. The quantitative determination of IL-1alpha, IL-2, IL-4, IL-6, IL-10, TNF-alpha and IFN-gamma was performed in ... More
T-helper and humoral responses to Puumala hantavirus nucleocapsid protein: identification of T-helper epitopes in a mouse model.
Authorsde Carvalho Nicacio C, Sällberg M, Hultgren C, Lundkvist A
JournalJ Gen Virol
PubMed ID11125166
'Puumala hantavirus (PUUV) is a rodent-borne agent causing nephropathia epidemica in humans, a milder form of haemorrhagic fever with renal syndrome occurring in Fennoscandia, central Europe and western Russia. In this study we characterized the immunogenicity of an E. coli-expressed nucleocapsid (N) protein of PUUV (strain Kazan-E6) in inbred mice ... More
Implication of STAT1 and STAT3 transcription factors in the response to superantigens.
AuthorsPlaza R, Vidal S, Rodriguez-Sanchez JL, Juarez C
JournalCytokine
PubMed ID14687580
'The activation of STAT1 and STAT3 in response to SEB was analyzed in spleen of Balb/c mice. The intraperitoneal injection of the superantigen SEB activated STAT1 and STAT3 in spleen. Activated STAT1 almost completely disappeared in 24 h even though activated STAT3 was present for more than 48 h after ... More
Retroviral gene therapy with an immunoglobulin-antigen fusion construct protects from experimental autoimmune uveitis.
AuthorsAgarwal RK, Kang Y, Zambidis E, Scott DW, Chan CC, Caspi RR
JournalJ Clin Invest
PubMed ID10903340
'Immunoglobulins can serve as tolerogenic carriers for antigens, and B cells can function as tolerogenic antigen-presenting cells. We used this principle to design a strategy for gene therapy of experimental autoimmune uveitis, a cell-mediated autoimmune disease model for human uveitis induced with the uveitogenic interphotoreceptor retinoid-binding protein (IRBP). A retroviral ... More
Effects of oral administration of Phellinus linteus on the production of Th1- and Th2-type cytokines in mice.
AuthorsOh GS, Lee MS, Pae HO, Kwon J, Lee SS, Jeong JG, Shin MK, Kwon TO, Chung HT
JournalImmunopharmacol Immunotoxicol
PubMed ID16873096
'The mushroom Phellinus linteus (PL) has been shown to have antitumor and immunostimulatory effects. We hypothesized that the hot water extract of PL (WEPL) exerts its significant immunostimulatory effect by inducing production of the Th1-derived cytokine interferon-gamma (IFN-gamma) by T lymphocytes. T lymphocytes were isolated from the mice fed with ... More
Adenovirus-mediated interleukin-18 mutant in vivo gene transfer inhibits tumor growth through the induction of T cell immunity and activation of natural killer cell cytotoxicity.
AuthorsHwang KS, Cho WK, Yoo J, Seong YR, Kim BK, Kim S, Im DS
JournalCancer Gene Ther
PubMed ID15044962
'We report here that gene transfer using recombinant adenoviruses encoding interleukin (IL)-18 mutants induces potent antitumor activity in vivo. The precursor form of IL-18 (ProIL-18) is processed by caspase-1 to produce bioactive IL-18, but its cleavage by caspase-3 (CPP32) produces an inactive form. To prepare IL-18 molecules with an effective ... More
Induction of cytotoxic T lymphocyte responses against hepatitis delta virus antigens which protect against tumor formation in mice.
AuthorsMauch C, Grimm C, Meckel S, Wands JR, Blum HE, Roggendorf M, Geissler M
JournalVaccine
PubMed ID11567762
'The cellular immune response is a crucial defense mechanism against hepatotropic viruses and in chronic viral hepatitis prevention. Moreover, hepatitis delta virus (HDV) immunogenicity may be an important component in the development of prophylactic and therapeutic vaccines. Therefore, we evaluated the immunogenicity of the small (HDAg) or large delta antigen ... More
L-carnitine modifies the humoral immune response in mice after in vitro or in vivo treatment.
AuthorsAthanassakis I, Mouratidou M, Sakka P, Evangeliou A, Spilioti M, Vassiliadis S
JournalInt Immunopharmacol
PubMed ID11562072
'Although the role of L-carnitine (L-Cn) as a cofactor in the oxidation of long-chain fatty acids has been well established, this agent has also been recognized to have an important role in the regulation of carbohydrate metabolism, and consequently, the maintenance of cell membrane structure and cell viability. L-Cn has ... More
Ontogenic development of Th1 and Th2 cytokine capabilities in random bred mice.
AuthorsFagoaga OR, Nehlsen-Cannarella SL
JournalDev Immunol
PubMed ID12353658
'Neonatal mouse Th1 capabilities mature by postnatal day 5. Neonatal T cells have been reported to exhibit a bias towards Th2 cytokine production when co-cultured with adult antigen presenting cells (APC). We studied mouse T cells co-cultured with contemporary APC to evaluate neonatal cytokine production capabilities. In response to allogeneic ... More
Immunity to murine breast cancer cells modified to express MUC-1, a human breast cancer antigen, in transgenic mice tolerant to human MUC-1.
AuthorsCarr-Brendel V, Markovic D, Ferrer K, Smith M, Taylor-Papadimitriou J, Cohen EP
JournalCancer Res
PubMed ID10811121
'The high incidence of breast cancer in women and the severity of the disease have stimulated a need for improved and novel forms of therapy. The product of the MUC-1 gene has been identified as a breast cancer-associated antigen in breast cancer patients. The gene has been cloned and sequenced. ... More
Transforming growth factor beta blocks Tec kinase phosphorylation, Ca2+ influx, and NFATc translocation causing inhibition of T cell differentiation.
AuthorsChen CH, Seguin-Devaux C, Burke NA, Oriss TB, Watkins SC, Clipstone N, Ray A
JournalJ Exp Med
PubMed ID12810687
'Transforming growth factor (TGF)-beta inhibits T cell proliferation and differentiation. TGF-beta has been shown to inhibit the expression of transcription factors such as GATA-3 and T-bet that play important roles in T cell differentiation. Here we show that TGF-beta inhibits T cell differentiation at a more proximal step. An early ... More
Medroxyprogesterone acetate enhances in vivo and in vitro antibody production.
AuthorsVermeulen M, Pazos P, Lanari C, Molinolo A, Gamberale R, Geffner JR, Giordano M
JournalImmunology
PubMed ID11576224
'In the present study we examine the effects of medroxyprogesterone acetate (MPA) on the specific antibody secretion to T-dependent antigens. Our results show that the in vivo administration of MPA to mice, 7 or 90 days before immunization with sheep red blood cells (SRBC), significantly enhanced both, primary and secondary ... More
DNA-based immunization breaks tolerance in a hepatitis C virus transgenic mouse model.
AuthorsEncke J, Geissler M, Stremmel W, Wands JR
JournalHum Vaccin
PubMed ID17012868
'Chronic hepatitis C infection (HCV) is associated with high morbidity and mortality due to limited treatment options. HCV transgenic mice can be used as surrogate model of chronic HCV infection and may therefore be a small animal model for the evaluation of therapeutic vaccination strategies. We immunized transgenic mice expressing ... More
Flow cytometric determination of cytokine production and proliferation in hepatitis B core antigen specific murine CD4 cells: lack of correlation between number of cytokine producing cells and cytokine levels in supernatant.
AuthorsAlheim M, Lazdina U, Milich DR, Sällberg M
JournalJ Immunol Methods
PubMed ID11684133
'Hepatitis B virus (HBV) core antigen (HBcAg) has extraordinary immunostimulatory properties. The majority of studies done so far on HBcAg induced responses have used ELISA or bioassay for cytokine determination and the 3[H]thymidine incorporation assay to measure proliferation. Here multiparameter flow cytometry was used to measure HBcAg induced cytokine production ... More
c-Cbl and Cbl-b regulate T cell responsiveness by promoting ligand-induced TCR down-modulation.
AuthorsNaramura M, Jang IK, Kole H, Huang F, Haines D, Gu H
JournalNat Immunol
PubMed ID12415267
'How Cbl family proteins regulate T cell responses is unclear. We found that c-Cbl Cbl-b double knock-out (dKO) T cells became hyperresponsive upon anti-CD3 stimulation, even though the major T cell antigen receptor (TCR) signaling pathways were not enhanced. The dKO T cells did not down-modulate surface TCR after ligand ... More
Induction of immune responses in mice by vaccination with Liposome-entrapped DNA complexes encoding Toxoplasma gondii SAG1 and ROP1 genes.
AuthorsChen H, Chen G, Zheng H, Guo H
JournalChin Med J (Engl)
PubMed ID14570624
To evaluate the immune responses induced by experimental DNA construct encoding Toxoplasma gondii (T. gondii) surface antigen1 (SAG1) and rhoptry protein 1 (ROP1) in mice as a hybrid gene. ... More
Prolactin modulates survival and cellular immune functions in septic mice.
AuthorsOberbeck R, Schmitz D, Wilsenack K, Schüler M, Biskup C, Schedlowski M, Nast-Kolb D, Exton MS
JournalJ Surg Res
PubMed ID12957137
The immunomodulatory properties of the pituitary hormone prolactin have been demonstrated. It was proposed that prolactin is important in maintaining normal immune response in several pathological states. We investigated the effect of prolactin administration on the survival and cellular immune functions during systemic inflammation. ... More
The effect of CD28/B7 blockade on alloreactive T and B cells after liver cell transplantation.
AuthorsReddy B, Gupta S, Chuzhin Y, Kalergis AM, Budhai L, Zhang M, Droguett G, Horwitz MS, Chowdhury JR, Nathenson SG, Davidson A
JournalTransplantation
PubMed ID11330546
Hepatocyte cell lines are beginning to be developed as universal donors for isolated liver cell transplantation, which is a less invasive method than orthotopic liver transplantation for treatment of metabolic liver disease. The immune response to isolated liver cell transplantation and its modification by costimulatory blockade are as yet not ... More
Ethanol inhibition: the humoral and cellular immune response to hepatitis C virus NS5 protein after genetic immunization.
AuthorsEncke J, Wands JR
JournalAlcohol Clin Exp Res
PubMed ID10924011
The combination of chronic hepatitis C virus (HCV) and ethanol may increase viral replication, impair cellular immunity, and result in severe and progressive liver disease. Because HCV nonstructural proteins play a major role in viral elimination, we examined the cellular and humoral immune responses after genetic immunization against NS5 in ... More
Immunization by Arg-gingipain A DNA vaccine protects mice against an invasive Porphyromonas gingivalis infection through regulation of interferon-gamma production.
AuthorsYonezawa H, Kato T, Kuramitsu HK, Okuda K, Ishihara K
JournalOral Microbiol Immunol
PubMed ID16101960
We previously demonstrated that a Porphyromonas gingivalis rgpA DNA vaccine induced protective immune responses against P. gingivalis infection in mice. In the present study, reduction in lethality against infection by lethal doses of P. gingivalis was observed in the rgpA DNA vaccine-immunized mice. Cytokine levels in the mouse model with ... More
Evidence that IgE molecules mediate a spectrum of effects on mast cell survival and activation via aggregation of the FcepsilonRI.
AuthorsKitaura J, Song J, Tsai M, Asai K, Maeda-Yamamoto M, Mocsai A, Kawakami Y, Liu FT, Lowell CA, Barisas BG, Galli SJ, Kawakami T
JournalProc Natl Acad Sci U S A
PubMed ID14569021
We demonstrate that binding of different IgE molecules (IgEs) to their receptor, FcepsilonRI, induces a spectrum of activation events in the absence of a specific antigen and provide evidence that such activation reflects aggregation of FcepsilonRI. Highly cytokinergic IgEs can efficiently induce production of cytokines and render mast cells resistant ... More
Exacerbated inflammatory responses in transgenic mice expressing an inhibitor of apoptosis protein (OpIAP).
AuthorsRobles MS, Leonardo E, Criado LM, Izquierdo M, Martínez-A C
JournalCell Death Differ
PubMed ID14576774
Members of the inhibitor of apoptosis protein family are involved not only in suppressing apoptosis, but also in signal transduction, cell division, and are associated with some types of cancers. Here we show that transgenic expression of the inhibitor of apoptosis protein OpIAP in murine T lymphocytes leads to a ... More
B7-DC, a new dendritic cell molecule with potent costimulatory properties for T cells.
AuthorsTseng SY, Otsuji M, Gorski K, Huang X, Slansky JE, Pai SI, Shalabi A, Shin T, Pardoll DM, Tsuchiya H
JournalJ Exp Med
PubMed ID11283156
Dendritic cells (DCs), unique antigen-presenting cells (APCs) with potent T cell stimulatory capacity, direct the activation and differentiation of T cells by providing costimulatory signals. As such, they are critical regulators of both natural and vaccine-induced immune responses. A new B7 family member, B7-DC, whose expression is highly restricted to ... More
T cell studies in a peptide-induced model of systemic lupus erythematosus.
AuthorsKhalil M, Inaba K, Steinman R, Ravetch J, Diamond B
JournalJ Immunol
PubMed ID11160209
We have previously reported that immunization with a peptide mimetope of dsDNA on a branched polylysine backbone (DWEYSVWLSN-MAP) induces a systemic lupus erythematosus-like syndrome in the nonautoimmune BALB/c mouse strain. To understand the mechanism underlying this breakdown in self tolerance, we examined the role of T cells in the response. ... More
Protective immunity induced by the full-length cDNA encoding paramyosin of Chinese Schistosoma japonicum.
AuthorsZhou S, Liu S, Song G, Xu Y, Sun W
JournalVaccine
PubMed ID10856799
The full-length cDNA encoding paramyosin of Chinese S. japonicum (Sjc97) has been cloned and sequenced for the first time. The homology of the nucleotide sequence of paramyosin and the deduced amino acid sequence between Chinese, Philippine, Japanese strains and S. mansoni (Sm97) were 99.7, 99.8 and 96% at amino acid ... More
Analysis of the effect of leptin on immune function in vivo using diet-induced obese mice.
AuthorsMito N, Yoshino H, Hosoda T, Sato K
JournalJ Endocrinol
PubMed ID14709155
Leptin can regulate several immune functions. However, the role of leptin on lymphocyte function has not been recognized in vivo. Accordingly, we have investigated the effect of leptin on starvation-induced immune dysfunction using diet-induced obese mice. To induce obesity, C57BL/6J mice were fed a high-fat diet for 14 weeks and ... More
CXCL10 (IFN-gamma-inducible protein-10) control of encephalitogenic CD4+ T cell accumulation in the central nervous system during experimental autoimmune encephalomyelitis.
Experimental autoimmune encephalomyelitis (EAE) is a CD4(+) Th1-mediated demyelinating disease of the CNS that serves as a model for multiple sclerosis. A critical event in the pathogenesis of EAE is the entry of both Ag-specific and Ag-nonspecific T lymphocytes into the CNS. In the present report, we investigated the role ... More
Regulation of dendritic cell differentiation and antitumor immune response in cancer by pharmacologic-selective inhibition of the janus-activated kinase 2/signal transducers and activators of transcription 3 pathway.
AuthorsNefedova Y, Nagaraj S, Rosenbauer A, Muro-Cacho C, Sebti SM, Gabrilovich DI
JournalCancer Res
PubMed ID16230418
Abnormal dendritic cell differentiation and accumulation of immunosuppressive myeloid cells in cancer is one of the major factors of tumor nonresponsiveness. We have previously shown that hyperactivation of the Janus-activated kinase 2/signal transducers and activators of transcription 3 (JAK2/STAT3) induced by tumor-derived factors (TDF) is responsible for abnormal dendritic cell ... More
Epitope-specific antibody response to Mel-CAM induced by mimotope immunization.
AuthorsHafner C, Wagner S, Jasinska J, Allwardt D, Scheiner O, Wolff K, Pehamberger H, Wiedermann U, Breiteneder H
JournalJ Invest Dermatol
PubMed ID15654965
Peptide mimotopes of tumor antigen epitopes have been proposed as components of tumor vaccines. In this study, we determined the immunogenicity of melcam mim1 and melcam mim2, peptide mimics of an epitope of the melanoma cell-adhesion molecule (Mel-CAM). BALB/c mice were vaccinated either with mimotopes or mimotopes coupled to tetanus ... More
Silencing OCILRP2 leads to intrinsic defects in T cells in response to antigenic stimulation.
AuthorsTian W, Feng B, Liou HC
JournalCell Immunol
PubMed ID16143319
We have previously demonstrated that OCILRP2 interaction with its ligand NKRP1f provides a co-stimulatory signal for optimal T cell proliferation and IL-2 production. Here, using RNA interference technology, we will demonstrate that silencing OCILRP2 in vivo leads to intrinsic impairment in T cell response to CD3- and CD28-cross-linking as well ... More
Enhanced signaling through the IL-2 receptor in CD8+ T cells regulated by antigen recognition results in preferential proliferation and expansion of responding CD8+ T cells rather than promotion of cell death.
AuthorsCheng LE, Ohlén C, Nelson BH, Greenberg PD
JournalProc Natl Acad Sci U S A
PubMed ID11867736
Multiple cytokines, including IL-2, can affect T cell proliferation and survival. However, IL-2 can lead to apoptosis as well as proliferation, making unclear whether IL-2 receptor (IL-2R) signals ultimately have a predominantly positive or negative effect. To address this issue, we examined the effect of enhancing IL-2R signals in CD8(+) ... More
Murine keratocytes function as antigen-presenting cells.
AuthorsSeo SK, Gebhardt BM, Lim HY, Kang SW, Higaki S, Varnell ED, Hill JM, Kaufman HE, Kwon BS
JournalEur J Immunol
PubMed ID11745349
Keratocytes express MHC class I molecules constitutively, and keratocytes stimulated with IFN-gamma express MHC class II molecules. Unstimulated keratocytes constitutively express B7-1 and ICAM-1, as well as low levels of CD40 and 4-1BBL. These findings indicate that keratocytes may deliver both antigen-specific and costimulatory signals to CD4(+) and CD8(+) T ... More
Effect of a single oral dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin on immune function in male NC/Nga mice.
AuthorsFujimaki H, Nohara K, Kobayashi T, Suzuki K, Eguchi-Kasai K, Tsukumo S, Kijima M, Tohyama C
JournalToxicol Sci
PubMed ID11861978
Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces immunosuppression in humans and animals. However, the effect of TCDD on Th2-type immune responses such as allergic reactions has been unclear. Using NC/Nga mice that developed atopic dermatitis-like skin lesions with marked elevation in plasma of total IgE when bred under conventional conditions, we investigated ... More
Insulin-induced remission in new-onset NOD mice is maintained by the PD-1-PD-L1 pathway.
AuthorsFife BT, Guleria I, Gubbels Bupp M, Eagar TN, Tang Q, Bour-Jordan H, Yagita H, Azuma M, Sayegh MH, Bluestone JA
JournalJ Exp Med
PubMed ID17116737
The past decade has seen a significant increase in the number of potentially tolerogenic therapies for treatment of new-onset diabetes. However, most treatments are antigen nonspecific, and the mechanism for the maintenance of long-term tolerance remains unclear. In this study, we developed an antigen-specific therapy, insulin-coupled antigen-presenting cells, to treat ... More
B-cell depletion fails to abrogate the induction of oral tolerance of specific Th1 immune responses in mice.
AuthorsPeng HJ, Chang ZN, Lee CC, Kuo SW
JournalScand J Immunol
PubMed ID10792836
Antigen presentation by resting B cells has been shown to induce peripheral tolerance to intravenous (i.v.) administered soluble protein antigens. We further examined the role of resting B cells in the induction of oral tolerance. Mice were treated continuously from birth with rabbit antimouse IgM serum for 5 weeks. Immunohistological ... More
Transient anti-CD154-mediated immunotherapy of ongoing relapsing experimental autoimmune encephalomyelitis induces long-term inhibition of disease relapses.
AuthorsHoward LM, Dal Canto MC, Miller SD
JournalJ Neuroimmunol
PubMed ID12161021
Relapsing experimental autoimmune encephalomyelitis (R-EAE) is a Th1-mediated central nervous system (CNS) autoimmune disease with pathology similar to that of relapsing-remitting multiple sclerosis. Among recent therapeutic approaches to prevent or treat relapsing disease is the strategic blockade of the CD154-CD40 ligand pair interactions. We have previously shown that CD154 blockade ... More
Enhanced delayed-type hypersensitivity and diminished immediate-type hypersensitivity in mice lacking the inducible VPAC(2) receptor for vasoactive intestinal peptide.
AuthorsGoetzl EJ, Voice JK, Shen S, Dorsam G, Kong Y, West KM, Morrison CF, Harmar AJ
JournalProc Natl Acad Sci U S A
PubMed ID11698667
Vasoactive intestinal peptide (VIP) and its G protein-coupled receptors, VPAC(1)R and VPAC(2)R, are prominent in the immune system and regulate many aspects of T cell-dependent immunity. In mouse T cells, VPAC(1)R is expressed constitutively, whereas VPAC(2)R is induced by immune stimuli. VPAC(2)R-null (VPAC(2)R(-/-)) mice on a C57BL/6 background are shown ... More
AuthorsLin CC, Chou CW, Shiau AL, Tu CF, Ko TM, Chen YL, Yang BC, Tao MH, Lai MD
JournalMol Ther
PubMed ID15294176
The therapeutic efficacy of HER2/c-erbB-2/neu DNA immunization on mouse tumor cells expressing exogenous human or rat p185neu but not on mouse tumor cells naturally expressing mouse p185neu has been demonstrated. We investigated the feasibility of using N-terminal rat neu DNA immunization on mouse tumor overexpressing endogenous p185neu and enhancing the ... More
Enhanced cellular delivery and transfection efficiency of plasmid DNA using positively charged biocompatible colloidal gold nanoparticles.
AuthorsNoh SM, Kim WK, Kim SJ, Kim JM, Baek KH, Oh YK
JournalBiochim Biophys Acta
PubMed ID17324519
Efficient and safe nonviral gene delivery systems are a prerequisite for the clinical application of therapeutic genes. In this study, we report an enhancement of the transfection efficiency of plasmid DNA, via the use of positively charged colloidal gold nanoparticles (PGN). Plasmid DNA encoding for murine interleukin-2 (pVAXmIL-2) was complexed ... More
In vivo transfection of a cis element 'decoy' against signal transducers and activators of transcription 6 (STAT6)-binding site ameliorates IgE-mediated late-phase reaction in an atopic dermatitis mouse model.
AuthorsYokozeki H, Wu MH, Sumi K, Awad S, Satoh T, Katayama I, Takeda K, Akira S, Kaneda Y, Nishioka K
JournalGene Ther
PubMed ID15306842
Signal transducers and activators of transcription 6 (STAT6) play a crucial role in the transactivation of IL-4 and IL-13, which might be involved in the pathogenesis of atopic dermatitis (AD). We herein reported that the IgE-mediated late-phase reaction significantly decreased in STAT6-deficient (STAT6(-/-)) mice in AD model mice induced by ... More
Targeted gene disruption of the heat shock protein 72 gene (hsp70.1) in the donor tissue is associated with a prolonged rejection-free survival in the murine skin allograft model.
AuthorsOh KH, Kim JY, Kim D, Lee EM, Oh HY, Seo JS, Han JS, Kim S, Lee JS, Ahn C
JournalTranspl Immunol
PubMed ID15589740
It is known that the expression level of the heat shock protein (HSP) is elevated in allograft tissues, but the specific role of the HSP in the acute rejection has not been elucidated. This study aims to determine how and when the HSP72 molecule works immunologically in the process of ... More
Testosterone receptor blockade restores cellular immunity in male mice after burn injury.
AuthorsMessingham KA, Shirazi M, Duffner LA, Emanuele MA, Kovacs EJ
JournalJ Endocrinol
PubMed ID11312147
Males are known to have increased risk for septic complications after traumatic injury, which appears to be mediated by the inhibitory effects of testosterone on immune function. The role of testosterone in immunity after burn injury, however, remains unclear. Herein, we examined the effects of a testosterone receptor antagonist, flutamide, ... More
Anti-ICAM-1 antibody and CTLA-4Ig synergistically enhance immature dendritic cells to induce donor-specific immune tolerance in vivo.
AuthorsWang Q, Zhang M, Ding G, Liu Y, Sun Y, Wang J, Zhang W, Fu Z, Cao X
JournalImmunol Lett
PubMed ID14611905
Immature dendritic cells (DC) have been demonstrated to induce T-cell hyporesponsiveness in vitro and immune tolerance in vivo. However, immature DC (iDC) may become mature once infused in vivo, thus limiting the prolongation of the allograft survival. Considering that mature DC express high level of B7, intercellular adhesion molecule-1 (ICAM-1), ... More
The IL-12 role in donor cell engraftment in a murine model of semiallogenic GVH disease with signs of autoimmune disease.
AuthorsGallego F, Gelpí C
JournalAutoimmunity
PubMed ID15293885
We analyzed the IL-12 effect in an autoimmune disease induced in a semiallogenic murine model of graft-vs-host disease (GVHD) Balb/c semiallogenic lymphoid cells i.v. infected in hybrid mice (Balb/c x A/J) F1 (CAF). IL-12 was administered 1 h before cell transplantation following two different protocols: (a) injecting 2 microg of ... More
Effective induction of immune tolerance by portal venous infusion with IL-10 gene-modified immature dendritic cells leading to prolongation of allograft survival.
AuthorsZhang M, Wang Q, Liu Y, Sun Y, Ding G, Fu Z, Min Z, Zhu Y, Cao X
JournalJ Mol Med (Berl)
PubMed ID15168681
Dendritic cells (DC) not only initiate T cell responses, but are also involved in the induction of tolerance. The functional properties of DC are strictly dependent on their state of maturation. It has been shown that immature DC can induce immune tolerance and prolong allograft survival. Interleukin-10 (IL-10) is an ... More
DNA vaccination with map1 gene followed by protein boost augments protection against challenge with Cowdria ruminantium, the agent of heartwater.
AuthorsNyika A, Barbet AF, Burridge MJ, Mahan SM
JournalVaccine
PubMed ID11803084
A DNA vaccine encoding the immunodominant MAP1 protein of Cowdria ruminantium (Crystal Springs (CS) strain) was shown to partially protect DBA/2 mice against homologous lethal challenge. To enhance the protective capacity of this DNA vaccine, the effects of length of interval between vaccinations and of prime-boost regimes were investigated. Increasing ... More
Nitric oxide sensitizes tumor cells to dendritic cell-mediated apoptosis, uptake, and cross-presentation.
AuthorsHuang J, Tatsumi T, Pizzoferrato E, Vujanovic N, Storkus WJ
JournalCancer Res
PubMed ID16166326
Dendritic cells are professional antigen-presenting cells associated with efficient antigen processing and presentation to T cells. However, recent evidence also suggests that dendritic cells may mediate direct tumoricidal functions. In this study, we investigated the mechanism by which murine dendritic cells mediate the apoptotic death of murine lymphoma cell lines, ... More