Antibody-mediated protection in murine Cryptococcus neoformans infection is associated with pleotrophic effects on cytokine and leukocyte responses.
AuthorsFeldmesser M, Mednick A, Casadevall A
JournalInfect Immun
PubMed ID11854246
Cryptococcus neoformans, an encapsulated yeast, is a common cause of life-threatening meningoencephalitis in immunosuppressed patients. We previously observed that administration of a monoclonal antibody (MAb) to the capsular polysaccharide to mice with pulmonary infection prolonged survival and enhanced granulomatous inflammation without reducing lung CFU. To understand the mechanism of MAb ... More
Changes in serum levels of cytokines in mice injected with an immunostimulator C3bgp isolated from Cuscuta europea.
AuthorsStanilova S, Dobreva Z, Zhelev Z
JournalInt Immunopharmacol
PubMed ID11515822
'The effect of C3 binding glycoprotein (C3bgp), isolated from Cuscuta europea seeds on induction of in vivo cytokine synthesis was investigated. Different groups of mice were stimulated with 30 microg C3bgp per mouse, injected intraperitoneally. The quantitative determination of IL-1alpha, IL-2, IL-4, IL-6, IL-10, TNF-alpha and IFN-gamma was performed in ... More
T-helper and humoral responses to Puumala hantavirus nucleocapsid protein: identification of T-helper epitopes in a mouse model.
Authorsde Carvalho Nicacio C, Sällberg M, Hultgren C, Lundkvist A
JournalJ Gen Virol
PubMed ID11125166
'Puumala hantavirus (PUUV) is a rodent-borne agent causing nephropathia epidemica in humans, a milder form of haemorrhagic fever with renal syndrome occurring in Fennoscandia, central Europe and western Russia. In this study we characterized the immunogenicity of an E. coli-expressed nucleocapsid (N) protein of PUUV (strain Kazan-E6) in inbred mice ... More
Intensification of antitumor effect by T helper 1-dominant adoptive immunogene therapy for advanced orthotopic colon cancer.
AuthorsNakamori M, Iwahashi M, Nakamura M, Ueda K, Zhang X, Yamaue H
JournalClin Cancer Res
PubMed ID12796406
'T helper (Th) 1/Th2 balance, controlled by Th1 or Th2 cells producing cytokines, plays important roles in antitumor immunity. Interleukin-18 (IL-18) can act together with IL-12 in promoting the generation of IFN-gamma producing Th1 cells. The goal of this study was to determine whether cytotoxic T lymphocyte (CTL) secreted in ... More
Inhibition of phosphoinositide 3-kinase delta attenuates allergic airway inflammation and hyperresponsiveness in murine asthma model.
AuthorsLee KS, Lee HK, Hayflick JS, Lee YC, Puri KD
JournalFASEB J
PubMed ID16507763
'P110delta phosphoinositide 3-kinase (PI3K) plays a pivotal role in the recruitment and activation of certain inflammatory cells. Recent findings revealed that the activity of p110delta also contributes to allergen-IgE-induced mast cell activation and vascular permeability. We investigated the role of p110delta in allergic airway inflammation and hyperresponsiveness using IC87114, a ... More
A role for CD1d-restricted NKT cells in injury-associated T cell suppression.
AuthorsFaunce DE, Gamelli RL, Choudhry MA, Kovacs EJ
JournalJ Leukoc Biol
PubMed ID12773507
'Natural killer T (NKT) cells are known to modulate T cell responses during autoimmunity, tolerance, and antitumor immunity; however, their potential role in regulating the immune response to injury has not been reported. Using a murine model of burn injury, we investigated whether CD1d-restricted NKT cells played a role in ... More
Selective loss of c-Rel compromises dendritic cell activation of T lymphocytes.
AuthorsBoffa DJ, Feng B, Sharma V, Dematteo R, Miller G, Suthanthiran M, Nunez R, Liou HC
JournalCell Immunol
PubMed ID12826080
'Dendritic cells initiate the immune response by presenting antigen in the context of varying levels of costimulation. The maturation state of the dendritic cell determines the quantity and quality (Th1, Th2) of the subsequent T cell response. Members of the NF-kappaB family of transcription factors have previously been implicated in ... More
Inhibition of IgE-production by peroxisome proliferator-activated receptor ligands.
AuthorsRühl R, Dahten A, Schweigert FJ, Herz U, Worm M
JournalJ Invest Dermatol
PubMed ID14632193
'In this study we analyzed the effect of peroxisome proliferator-activated receptor-alpha and -gamma ligands on immunoglobulin synthesis and cytokine production in non-allergic and atopic dermatitis donors in vitro, but also in vivo ovalbumin-sensitized mice. A significant inhibition in CD40+ interleukin-4-mediated, but also basal IgE synthesis from peripheral blood mononuclear cells ... More
Regulation of immature dendritic cell migration by RhoA guanine nucleotide exchange factor Arhgef5.
AuthorsWang Z, Kumamoto Y, Wang P, Gan X, Lehmann D, Smrcka AV, Cohn L, Iwasaki A, Li L, Wu D
JournalJ Biol Chem
PubMed ID19713215
'There are a large number of Rho guanine nucleotide exchange factors, most of which have no known functions. Here, we carried out a short hairpin RNA-based functional screen of Rho-GEFs for their roles in leukocyte chemotaxis and identified Arhgef5 as an important factor in chemotaxis of a macrophage phage-like RAW264.7 ... More
Nippocystatin, a cysteine protease inhibitor from Nippostrongylus brasiliensis, inhibits antigen processing and modulates antigen-specific immune response.
AuthorsDainichi T, Maekawa Y, Ishii K, Zhang T, Nashed BF, Sakai T, Takashima M, Himeno K
JournalInfect Immun
PubMed ID11705911
'During infection, parasites evade the host immune system by modulating or exploiting the immune system; e.g., they suppress expression of major histocompatibility complex class II molecules or secrete cytokine-like molecules. However, it is not clear whether helminths disturb the immune responses of their hosts by controlling the antigen-processing pathways of ... More
Retroviral gene therapy with an immunoglobulin-antigen fusion construct protects from experimental autoimmune uveitis.
AuthorsAgarwal RK, Kang Y, Zambidis E, Scott DW, Chan CC, Caspi RR
JournalJ Clin Invest
PubMed ID10903340
'Immunoglobulins can serve as tolerogenic carriers for antigens, and B cells can function as tolerogenic antigen-presenting cells. We used this principle to design a strategy for gene therapy of experimental autoimmune uveitis, a cell-mediated autoimmune disease model for human uveitis induced with the uveitogenic interphotoreceptor retinoid-binding protein (IRBP). A retroviral ... More
Possible role of autoantibodies in the pathophysiology of GM2 gangliosidoses.
AuthorsYamaguchi A, Katsuyama K, Nagahama K, Takai T, Aoki I, Yamanaka S
JournalJ Clin Invest
PubMed ID14722612
'Mice containing a disruption of the Hexb gene have provided a useful model system for the study of the human lysosomal storage disorder known as Sandhoff disease (SD). Hexb(-/-) mice rapidly develop a progressive neurologic disease of ganglioside GM2 and GA2 storage. Our study revealed that the disease states in ... More
Immunization with the Chlamydia trachomatis major outer membrane protein, using the outer surface protein A of Borrelia burgdorferi as an adjuvant, can induce protection against a chlamydial genital challenge.
AuthorsPal S, Luke CJ, Barbour AG, Peterson EM, de la Maza LM
JournalVaccine
PubMed ID12615442
'Two strains of mice C3H/HeN (H-2(k)) and BALB/c (H-2(d)) were immunized with the Chlamydia trachomatis mouse pneumonitis (MoPn) major outer membrane protein (MOMP) using the Borrelia burgdorferi outer surface protein A (OspA) as an adjuvant. As a control, groups of mice were inoculated with ovalbumin instead of MOMP. Female mice ... More
Relationship between the immune response and protection conferred by new designed inactivated vaccines against ovine enzootic abortion in a mouse model.
AuthorsCaro MR, Ortega N, Buendía AJ, Gallego MC, Del Río L, Cuello F, Salinas J
JournalVaccine
PubMed ID12804839
'Chlamydophila abortus is a gram-negative obligate intracellular bacterium and the etiological agent of ovine enzootic abortion (OEA), an economically important disease in many countries. Inactivated vaccines have been reported to induce immunity in ewes and they have been used for many years. However, some outbreaks have been reported in correctly ... More
LF15-0195 generates tolerogenic dendritic cells by suppression of NF-kappaB signaling through inhibition of IKK activity.
AuthorsYang J, Bernier SM, Ichim TE, Li M, Xia X, Zhou D, Huang X, Strejan GH, White DJ, Zhong R, Min WP
JournalJ Leukoc Biol
PubMed ID12949248
'LF15-0195 (LF) is a potent, less toxic analog of the immunosuppressant 15-deoxyspergualine, which we previously reported to prevent graft rejection and to induce permanent tolerance in a murine cardiac transplantation model. However, the underlying mechanism of action of LF required elucidation. In this study, dendritic cells (DC) treated with LF ... More
Overexpression of interleukin-4 in lungs of mice impairs elimination of Histoplasma capsulatum.
'Protection against the pathogenic fungus Histoplasma capsulatum requires Th1 cytokines. Since interleukin-4 (IL-4) can inhibit both Th1 cytokine production and activity, we examined the effects of overproduction of IL-4 in the lung on the course of pulmonary histoplasmosis. IL-4 lung transgenic mice manifested a higher fungal burden in their lungs, ... More
Effects of oral administration of Phellinus linteus on the production of Th1- and Th2-type cytokines in mice.
AuthorsOh GS, Lee MS, Pae HO, Kwon J, Lee SS, Jeong JG, Shin MK, Kwon TO, Chung HT
JournalImmunopharmacol Immunotoxicol
PubMed ID16873096
'The mushroom Phellinus linteus (PL) has been shown to have antitumor and immunostimulatory effects. We hypothesized that the hot water extract of PL (WEPL) exerts its significant immunostimulatory effect by inducing production of the Th1-derived cytokine interferon-gamma (IFN-gamma) by T lymphocytes. T lymphocytes were isolated from the mice fed with ... More
Enhanced UVfemale1 tumor growth in CBF1 mice infected with Schistosoma mansoni due to modulation of Th1-like responses.
AuthorsYoshida A, Maruyama H, Kumagai T, Amano T, Kobayashi F, Wang J, Kuribayashi K, Ohta N
JournalParasitol Int
PubMed ID12113756
'Effects of Schistosoma mansoni infection on anti-tumor immunity were examined in CBF1 mice with ultraviolet-induced UVfemale1 fibrosarcoma cells. Although many laboratory established tumor cells had rejection mechanisms independent of CD4(+) T cells, we confirmed that CD4(+) cells had significant roles in rejection of UVfemale1 cells in the syngeneic CBF1 mice. ... More
Immunization with the Chlamydia trachomatis mouse pneumonitis major outer membrane protein can elicit a protective immune response against a genital challenge.
AuthorsPal S, Theodor I, Peterson EM, de la Maza LM
JournalInfect Immun
PubMed ID11553566
'Infertility, ectopic pregnancy, and chronic abdominal pain are frequent complications of genital infections with Chlamydia trachomatis. In an attempt to produce a vaccine to protect against this pathogen we purified and refolded the C. trachomatis mouse pneumonitis (MoPn) major outer membrane protein (MOMP). This preparation, mixed with Freund's adjuvant using ... More
Induction of cytotoxic T lymphocyte responses against hepatitis delta virus antigens which protect against tumor formation in mice.
AuthorsMauch C, Grimm C, Meckel S, Wands JR, Blum HE, Roggendorf M, Geissler M
JournalVaccine
PubMed ID11567762
'The cellular immune response is a crucial defense mechanism against hepatotropic viruses and in chronic viral hepatitis prevention. Moreover, hepatitis delta virus (HDV) immunogenicity may be an important component in the development of prophylactic and therapeutic vaccines. Therefore, we evaluated the immunogenicity of the small (HDAg) or large delta antigen ... More
Evaluation of the immunogenicity of the P97R1 adhesin of Mycoplasma hyopneumoniae as a mucosal vaccine in mice.
'The immunogenicity of P97 adhesin repeat region R1 (P97R1) of Mycoplasma hyopneumoniae, an important pathogenesis-associated region of P97, was evaluated in mice as a mucosal vaccine. Mice were immunized orally with attenuated Salmonella typhimurium aroA strain CS332 harbouring a eukaryotic or prokaryotic expression vector encoding P97R1. Local and systemic immune ... More
Ontogenic development of Th1 and Th2 cytokine capabilities in random bred mice.
AuthorsFagoaga OR, Nehlsen-Cannarella SL
JournalDev Immunol
PubMed ID12353658
'Neonatal mouse Th1 capabilities mature by postnatal day 5. Neonatal T cells have been reported to exhibit a bias towards Th2 cytokine production when co-cultured with adult antigen presenting cells (APC). We studied mouse T cells co-cultured with contemporary APC to evaluate neonatal cytokine production capabilities. In response to allogeneic ... More
Immunity to murine breast cancer cells modified to express MUC-1, a human breast cancer antigen, in transgenic mice tolerant to human MUC-1.
AuthorsCarr-Brendel V, Markovic D, Ferrer K, Smith M, Taylor-Papadimitriou J, Cohen EP
JournalCancer Res
PubMed ID10811121
'The high incidence of breast cancer in women and the severity of the disease have stimulated a need for improved and novel forms of therapy. The product of the MUC-1 gene has been identified as a breast cancer-associated antigen in breast cancer patients. The gene has been cloned and sequenced. ... More
Preventing autoimmune arthritis using antigen-specific immature dendritic cells: a novel tolerogenic vaccine.
AuthorsPopov I, Li M, Zheng X, San H, Zhang X, Ichim TE, Suzuki M, Feng B, Vladau C, Zhong R, Garcia B, Strejan G, Inman RD, Min WP
JournalArthritis Res Ther
PubMed ID16911769
'Conventional treatments for autoimmune diseases have relied heavily on nonspecific immune suppressants, which possess a variety of adverse effects without inhibiting the autoimmune process in a specific manner. In the present study we demonstrate the effectiveness of antigen-specific, maturation-resistant, tolerogenic dendritic cells (DC) in suppressing collagen-induced arthritis, a murine model ... More
Intratumoral administration of immature dendritic cells following the adenovirus vector encoding CD40 ligand elicits significant regression of established myeloma.
AuthorsLiu Y, Xia D, Li F, Zheng C, Xiang J
JournalCancer Gene Ther
PubMed ID15565183
'Our previous study showed that J558 myeloma cells engineered CD40L lost their tumorigenicity in syngeneic mice, and the inoculation of J558/CD40L tumor cells further led to the protective immunity against wild tumors. In the present study, we investigated whether the vaccine can exert more efficient antitumor immunity by combination with ... More
Topical DNA vaccination with DNA/Lipid based complex.
AuthorsChoi MJ, Kim JH, Maibach HI
JournalCurr Drug Deliv
PubMed ID16472092
'Topical DNA vaccines have been shown to elicit both broad humoral and cellular immune response in vivo. The skin is an attractive site for the delivery DNA antigens for DNA vaccination. However, due to skin''s barrier properties, the penetration of DNA and the applications of topical vaccination are limited. To ... More
Prevention of acute and chronic allograft rejection by a novel retinoic acid receptor-alpha-selective agonist.
AuthorsSeino K, Yamauchi T, Shikata K, Kobayashi S, Nagai M, Taniguchi M, Fukao K
JournalInt Immunol
PubMed ID15096490
'To investigate the involvement of retinoic acid receptor (RAR)-alpha in allograft rejection, we investigated the effect of a novel selective agonist to the receptor, ER-38925, in a mouse cardiac allograft model. Prophylactic treatment with ER-38925 inhibited the acute rejection of the mouse cardiac allograft (BALB/c --> C3H/HeN) at 0.3 and ... More
Delta1-Notch3 interactions bias the functional differentiation of activated CD4+ T cells.
AuthorsMaekawa Y, Tsukumo S, Chiba S, Hirai H, Hayashi Y, Okada H, Kishihara K, Yasutomo K
JournalImmunity
PubMed ID14563319
'Following activation by antigen, naive CD4+ T helper precursor cells execute distinct genetic programs that result in their differentiation toward the type 1 or type 2 helper T cell (Th1 or Th2) phenotype. Although the differentiation and function of these Th subsets has been well studied, little is known about ... More
Parasite-induced Th2 polarization is associated with down-regulated dendritic cell responsiveness to Th1 stimuli and a transient delay in T lymphocyte cycling.
AuthorsJankovic D, Kullberg MC, Caspar P, Sher A
JournalJ Immunol
PubMed ID15294955
'The nature of the signals that bias Th effector choice is still not completely understood. Using parasite extracts from pathogens known to induce polarized Th1 or Th2 responses and an in vitro experimental model for priming murine CD4(+) cells, we demonstrated that splenic dendritic cells (DC), but not B cells, ... More
Immunotherapy with CpG DNA conjugated with T-cell epitope peptide of an allergenic Cry j 2 protein is useful for control of allergic conditions in mice.
AuthorsSuzuki M, Ohta N, Min WP, Matsumoto T, Min R, Zhang X, Toida K, Murakami S
JournalInt Immunopharmacol
PubMed ID17161816
'Immunotherapy using T-cell epitope peptides or CpG DNA conjugated with allergenic protein is useful, although the mechanisms of these therapies differ. However, the combination of CpG DNA and peptide, but not protein, had not been documented. Therefore, we investigated CpG DNA conjugated with peptide to obtain positive synergistic effects. In ... More
Immune responses generated by intramuscular DNA immunization of Brugia malayi transglutaminase (BmTGA) in mice.
'An attempt was made to evaluate the immunoprophylactic efficacy of Brugia malayi transglutaminase (BmTGA) as a DNA vaccine, for human lymphatic filariasis. BmTGA was cloned and characterized in the DNA vaccine vector pVAX1. Further, the tissue distribution study of the DNA construct, pVAX-TGA was carried out in mice and the ... More
Disruption of antigen-induced airway inflammation and airway hyper-responsiveness in low affinity neurotrophin receptor p75 gene deficient mice.
AuthorsTokuoka S, Takahashi Y, Masuda T, Tanaka H, Furukawa S, Nagai H
JournalBr J Pharmacol
PubMed ID11724766
'1. Recently, much attention has been paid to the relationship between the nervous and immune systems. The present study was conducted to clarify the role of neurotrophin low affinity receptor (p75N) in allergic airway inflammation and hyper-responsiveness (AHR) in mice by employing p75N gene deficient mice. 2. Mice were immunized ... More
Control of IgE and selective T(H)1 and T(H)2 cytokines by PG102 isolated from Actinidia arguta.
AuthorsPark EJ, Kim B, Eo H, Park K, Kim Y, Lee HJ, Son M, Chang YS, Cho SH, Kim S, Jin M
JournalJ Allergy Clin Immunol
PubMed ID16275390
'Various allergic responses are thought to result from the unbalanced development of T(H)1 and T(H)2 pathways and, subsequently, the overproduction of IgE. Therefore the modulation of T(H)1 and T(H)2 responses is a rational strategy for the treatment of allergic diseases.' ... More
The role of Foxp3+ T cells in long-term efficacy of prophylactic and therapeutic mucosal tolerance induction in mice.
AuthorsWinkler B, Hufnagl K, Spittler A, Ploder M, Kállay E, Vrtala S, Valenta R, Kundi M, Renz H, Wiedermann U
JournalAllergy
PubMed ID16409192
'Mucosal tolerance induction is suggested as treatment strategy for allergic diseases. Using a murine model of birch pollen (BP) allergy we investigated the long-term efficacy and the underlying mechanisms of mucosal tolerance induction with two structurally different molecules in a prophylactic and in a therapeutic set-up.' ... More
Medroxyprogesterone acetate enhances in vivo and in vitro antibody production.
AuthorsVermeulen M, Pazos P, Lanari C, Molinolo A, Gamberale R, Geffner JR, Giordano M
JournalImmunology
PubMed ID11576224
'In the present study we examine the effects of medroxyprogesterone acetate (MPA) on the specific antibody secretion to T-dependent antigens. Our results show that the in vivo administration of MPA to mice, 7 or 90 days before immunization with sheep red blood cells (SRBC), significantly enhanced both, primary and secondary ... More
DNA-based immunization breaks tolerance in a hepatitis C virus transgenic mouse model.
AuthorsEncke J, Geissler M, Stremmel W, Wands JR
JournalHum Vaccin
PubMed ID17012868
'Chronic hepatitis C infection (HCV) is associated with high morbidity and mortality due to limited treatment options. HCV transgenic mice can be used as surrogate model of chronic HCV infection and may therefore be a small animal model for the evaluation of therapeutic vaccination strategies. We immunized transgenic mice expressing ... More
Granulocyte/macrophage-colony stimulating factor produced by recombinant avian poxviruses enriches the regional lymph nodes with antigen-presenting cells and acts as an immunoadjuvant.
AuthorsKass E, Panicali DL, Mazzara G, Schlom J, Greiner JW
JournalCancer Res
PubMed ID11196163
'Recombinant avian poxviruses [fowlpox and canarypox (ALVAC)], restricted for replication in nonavian cell substrates and expressing granulocyte/macrophage-colony stimulating factor (avipox-GM-CSF), were evaluated for their ability to enrich an immunization site with antigen-presenting cells (APCs) and, in turn, function as biological vaccine adjuvants. Avipox-GM-CSF administered as a single s.c. injection significantly ... More
Mucosal co-application of lactic acid bacteria and allergen induces counter-regulatory immune responses in a murine model of birch pollen allergy.
AuthorsRepa A, Grangette C, Daniel C, Hochreiter R, Hoffmann-Sommergruber K, Thalhamer J, Kraft D, Breiteneder H, Mercenier A, Wiedermann U
JournalVaccine
PubMed ID14604575
'Recent epidemiological studies and clinical trials suggest a possible role of certain lactic acid bacteria (LAB) strains in the prevention of allergic diseases. In this study, we aimed at evaluating the immunomodulatory potential of two LAB strains, Lactococcus lactis and Lactobacillus plantarum, for prophylaxis and therapy of allergic immune responses. ... More
The Leishmania infantum acidic ribosomal protein P0 administered as a DNA vaccine confers protective immunity to Leishmania major infection in BALB/c mice.
AuthorsIborra S, Soto M, Carrión J, Nieto A, Fernández E, Alonso C, Requena JM
JournalInfect Immun
PubMed ID14573678
'In this study, we examined the immunogenic properties of the Leishmania infantum acidic ribosomal protein P0 (LiP0) in the BALB/c mouse model. The humoral and cellular responses induced by the administration of the LiP0 antigen, either as soluble recombinant LiP0 (rLiP0) or as a plasmid DNA formulation (pcDNA3-LiP0), were determined. ... More
Regulation of lymphocyte clustering by CD30-mediated ICAM-1 up-regulation.
AuthorsNam SY, Cho KS, Heo YM, Ha JC, Kim YH, Keun Yi H, Han Hwang P, Kim HM, Podack ER
JournalCell Immunol
PubMed ID12473266
'CD30 is expressed transiently on activated B and T lymphocytes and constitutively on several B- and T cell lymphomas. CD30 functions include participation in negative selection of thymocytes, costimulation of activated T cells, isotype switching of B cells, and regulation of the effector activity of cytotoxic lymphocytes. Although CD30 is ... More
CD4-dependent signaling is required for a late checkpoint during Th2 development associated with resistance to activation-induced cell death.
AuthorsTatari-Calderone Z, Brogdon JL, Tinsley KW, Ramezani A, Leitenberg D
JournalJ Immunol
PubMed ID16237052
'Previous studies have found that class II-restricted T cells from CD4-deficient mice reconstituted with a tail-less CD4 transgene have a specific defect in the development of Th2 effector cells; however, the reason for this defect was not clear. Following stimulation with a high potency peptide and exogenous IL-4, CD4-dependent signaling ... More
Flow cytometric determination of cytokine production and proliferation in hepatitis B core antigen specific murine CD4 cells: lack of correlation between number of cytokine producing cells and cytokine levels in supernatant.
AuthorsAlheim M, Lazdina U, Milich DR, Sällberg M
JournalJ Immunol Methods
PubMed ID11684133
'Hepatitis B virus (HBV) core antigen (HBcAg) has extraordinary immunostimulatory properties. The majority of studies done so far on HBcAg induced responses have used ELISA or bioassay for cytokine determination and the 3[H]thymidine incorporation assay to measure proliferation. Here multiparameter flow cytometry was used to measure HBcAg induced cytokine production ... More
Interferon gamma stabilizes the T helper cell type 1 phenotype.
AuthorsZhang Y, Apilado R, Coleman J, Ben-Sasson S, Tsang S, Hu-Li J, Paul WE, Huang H
JournalJ Exp Med
PubMed ID11457891
'T helper cell (Th)1-primed CD4 T cells from wild-type donors make little interleukin (IL)-4 when restimulated under Th2 conditions. However, such restimulation of Th1-primed cells from interferon (IFN)-gamma(2/-) or IFN-gamma receptor (IFN-gammaR)(-/-) mice resulted in substantial production of IL-4 and other Th2 cytokines. Adding IFN-gamma to the priming culture markedly ... More
A role for balance of interferon-gamma and interleukin-4 production in protective immunity against Neospora caninum infection.
AuthorsNishikawa Y, Inoue N, Makala L, Nagasawa H
JournalVet Parasitol
PubMed ID14559161
'A suitable balance in the production of Th1/Th2-type cytokines has a crucial role in the control of microbial infections. We investigated cytokine production patterns and effects during Neospora caninum infection, based on two mouse models and an in vitro system. In the acute infection of N. caninum, BALB/c-background IFN-gamma-deficient mice ... More
Enhancement of DNA vaccine potency through linkage of antigen to filamentous bacteriophage coat protein III domain I.
AuthorsCuesta AM, Suárez E, Larsen M, Jensen KB, Sanz L, Compte M, Kristensen P, Alvarez-Vallina L
JournalImmunology
PubMed ID16556264
'Although DNA-based cancer vaccines have been successfully tested in mouse models, a major drawback of cancer vaccination still remains, namely that tumour antigens are weak and fail to generate a vigorous immune response in tumour-bearing patients. Genetic technology offers strategies for promoting immune pathways by adding immune-activating genes to the ... More
Vaccination of newborn mice induces a strong protective immune response against respiratory and genital challenges with Chlamydia trachomatis.
AuthorsPal S, Peterson EM, de la Maza LM
JournalVaccine
PubMed ID16085340
'Chlamydia trachomatis infections can occur early in life and may result in long-term sequelae. To assess the feasibility of implementing a vaccine in newborns, groups of 2-day-old BALB/c mice were immunized intranasally (i.n.) with 1x10(4) inclusion forming units (IFU) of C. trachomatis mouse pneumonitis (MoPn). As a control, newborn mice ... More
c-Cbl and Cbl-b regulate T cell responsiveness by promoting ligand-induced TCR down-modulation.
AuthorsNaramura M, Jang IK, Kole H, Huang F, Haines D, Gu H
JournalNat Immunol
PubMed ID12415267
'How Cbl family proteins regulate T cell responses is unclear. We found that c-Cbl Cbl-b double knock-out (dKO) T cells became hyperresponsive upon anti-CD3 stimulation, even though the major T cell antigen receptor (TCR) signaling pathways were not enhanced. The dKO T cells did not down-modulate surface TCR after ligand ... More
Dendritic cells engineered to express the Flt3 ligand stimulate type I immune response, and induce enhanced cytoxic T and natural killer cell cytotoxicities and antitumor immunity.
AuthorsLiu Y, Huang H, Chen Z, Zong L, Xiang J
JournalJ Gene Med
PubMed ID12898636
Tumor antigen presentation by dendritic cells (DCs) to T cells in lymphoid organs is crucial for induction of antitumor immune responses. Fms-like tyrosine kinase 3 ligand (Flt3L) is a regulator of hematopoietic cell development. ... More
Resting B cells are not antigen-presenting cells in the induction of oral tolerance of specific Th2 immune responses in mice.
AuthorsPeng HJ, Chang ZN, Kuo SW, Lee CC, Tzau YY
JournalInt Arch Allergy Immunol
PubMed ID10899760
It has been shown that antigen presentation by resting B cells can induce tolerance to intravenously administered protein antigens, but the role of resting B cells in the induction of oral tolerance is unclear. ... More
Combination B7-Fc fusion protein treatment and Treg cell depletion therapy.
AuthorsLiu A, Hu P, Khawli LA, Epstein AL
JournalClin Cancer Res
PubMed ID16322313
A B7.1 fusion protein consisting of the extracellular domains of human B7.1 and the Fc portion of human IgG1, called B7.1-Fc, was generated and evaluated for its antitumor potential when used alone or in combination with regulatory T (Treg) cell depletion. ... More
Uteroglobin-related protein 1 expression suppresses allergic airway inflammation in mice.
AuthorsChiba Y, Kurotani R, Kusakabe T, Miura T, Link BW, Misawa M, Kimura S
JournalAm J Respir Crit Care Med
PubMed ID16456148
Uteroglobin-related protein (UGRP) 1, which is highly expressed in the epithelial cells of the airways, has been suggested to play a role in lung inflammation.
Protective effect of DNA-mediated immunization with a combination of SAG1 and IL-2 gene adjuvant against infection of Toxoplasma gondii in mice.
AuthorsChen G, Chen H, Guo H, Zheng H
JournalChin Med J (Engl)
PubMed ID12490084
To characterize the immune response induced by SAG1 encoding plasmid combined with IL-2 gene adjuvant in mice and to assess the protective effect of this vaccination against toxoplasmosis. ... More
Analyses of a mouse model of the dermatitis caused by 2,4,6-trinitro-1-chlorobenzene (TNCB)-repeated application.
AuthorsHarada D, Takada C, Tsukumo Y, Takaba K, Manabe H
JournalJ Dermatol Sci
PubMed ID15734285
The model of chronic dermatitis caused by repeated application of hapten is frequently used as a tool for assessment of the efficacy of a compound or the elucidation of chronic dermatitis. ... More
Induction of immune responses in mice by vaccination with Liposome-entrapped DNA complexes encoding Toxoplasma gondii SAG1 and ROP1 genes.
AuthorsChen H, Chen G, Zheng H, Guo H
JournalChin Med J (Engl)
PubMed ID14570624
To evaluate the immune responses induced by experimental DNA construct encoding Toxoplasma gondii (T. gondii) surface antigen1 (SAG1) and rhoptry protein 1 (ROP1) in mice as a hybrid gene. ... More
Oral administration of an edible-mushroom-derived protein inhibits the development of food-allergic reactions in mice.
AuthorsHsieh KY, Hsu CI, Lin JY, Tsai CC, Lin RH
JournalClin Exp Allergy
PubMed ID14616874
Food allergy is a common disease without effective treatment. Since strict elimination of food allergens may be difficult, strategies for effective intervention are urgently needed.
IL-5 deficiency abolishes aspects of airway remodelling in a murine model of lung inflammation.
AuthorsTrifilieff A, Fujitani Y, Coyle AJ, Kopf M, Bertrand C
JournalClin Exp Allergy
PubMed ID11422160
Lung remodelling is a recognized feature of chronic asthma. In the present study, we have used IL-5-deficient mice to evaluate the role of this cytokine and eosinophilic inflammation in the initial stages of the structural changes occurring in the lung after antigen challenge. ... More
Differential roles for CD4 and CD8 T cells after diisocyanate sensitization: genetic control of TH2-induced lung inflammation.
AuthorsHerrick CA, Das J, Xu L, Wisnewski AV, Redlich CA, Bottomly K
JournalJ Allergy Clin Immunol
PubMed ID12743574
Exposure to diisocyanates is a major cause of occupational asthma. We previously developed a novel mouse model of diisocyanate-induced asthma involving epicutaneous sensitization to hexamethylene diisocyanate (HDI) that demonstrates many features of the human disease, including airway eosinophilia and mucus hypersecretion. ... More
Suppression of Th2 immune responses by mekabu fucoidan from Undaria pinnatifida sporophylls.
AuthorsMaruyama H, Tamauchi H, Hashimoto M, Nakano T
JournalInt Arch Allergy Immunol
PubMed ID15970637
We demonstrated that mekabu fucoidan obtained from Undaria pinnatifida (Up) sporophylls augments the type 1 T-helper (Th1) cell response in normal BALB/c mice. In this study, we examined the effects of the fucoidan of mekabu on the type 2 T-helper (Th2) response in bronchoalveolar lavage fluid (BALF) after ovalbumin (OVA) ... More
The effect of CD28/B7 blockade on alloreactive T and B cells after liver cell transplantation.
AuthorsReddy B, Gupta S, Chuzhin Y, Kalergis AM, Budhai L, Zhang M, Droguett G, Horwitz MS, Chowdhury JR, Nathenson SG, Davidson A
JournalTransplantation
PubMed ID11330546
Hepatocyte cell lines are beginning to be developed as universal donors for isolated liver cell transplantation, which is a less invasive method than orthotopic liver transplantation for treatment of metabolic liver disease. The immune response to isolated liver cell transplantation and its modification by costimulatory blockade are as yet not ... More
Ethanol inhibition: the humoral and cellular immune response to hepatitis C virus NS5 protein after genetic immunization.
AuthorsEncke J, Wands JR
JournalAlcohol Clin Exp Res
PubMed ID10924011
The combination of chronic hepatitis C virus (HCV) and ethanol may increase viral replication, impair cellular immunity, and result in severe and progressive liver disease. Because HCV nonstructural proteins play a major role in viral elimination, we examined the cellular and humoral immune responses after genetic immunization against NS5 in ... More
Effects of Maharishi Amrit Kalash 5 as an Ayurvedic herbal food supplement on immune functions in aged mice.
AuthorsInaba R, Mirbod SM, Sugiura H
JournalBMC Complement Altern Med
PubMed ID15790423
Maharishi Amrit Kalash (MAK) 5, one of the Ayurvedic food supplements, belongs to a group of substances known as Rasayana. MAK5 and other Rasayanas are believed to enhance the body's resistance to infections and disease, and enhance longevity. In this study, we determined the effects of administration of MAK5, one ... More
Protective efficacy of vaccination by recombinant vaccinia virus against Neospora caninum infection.
AuthorsNishikawa Y, Inoue N, Xuan X, Nagasawa H, Igarashi I, Fujisaki K, Otsuka H, Mikami T
JournalVaccine
PubMed ID11163660
The recombinant vaccinia viruses expressing the surface protein of Neospora caninum tachyzoite, NcSAG1 or NcSRS2, were constructed. The vaccination with these recombinant viruses could protect effectively the parasite invasion in a mouse model system. The vaccine efficacy of NcSRS2 was higher than that of NcSAG1. The present study indicated that ... More
Expression of human immunodeficiency virus type 1 tat from a replication-deficient herpes simplex type 1 vector induces antigen-specific T cell responses.
AuthorsBozac A, Berto E, Vasquez F, Grandi P, Caputo A, Manservigi R, Ensoli B, Marconi P
JournalVaccine
PubMed ID16884834
Herpes simplex type-1 virus (HSV-1) based vectors have been widely used in different gene therapy approaches and also as experimental vaccines against HSV-1 infection. Recent advances in the HSV-1 technology do support the use of replication defective HSV-1 as vaccine vectors for delivery of foreign antigens. We have examined the ... More
The effects of Morinda citrifolia L. (noni) on the immune system: its molecular mechanisms of action.
AuthorsPalu AK, Kim AH, West BJ, Deng S, Jensen J, White L
JournalJ Ethnopharmacol
PubMed ID18063495
The aim of this study was to investigate the mechanisms involved in the immunomodulatory effects of Morinda citrifolia L. (noni) in vitro and in vivo in mice. In vitro, Tahitian Noni Juice (TNJ) and Noni fruit juice concentrates (NFJC) (1, 5mg/mL) potently activate cannabinoid 2 (CB2), but inhibit cannabinoid 1 ... More
Vaxfectin enhances antigen specific antibody titers and maintains Th1 type immune responses to plasmid DNA immunization.
AuthorsReyes L, Hartikka J, Bozoukova V, Sukhu L, Nishioka W, Singh G, Ferrari M, Enas J, Wheeler CJ, Manthorpe M, Wloch MK
JournalVaccine
PubMed ID11395213
Antigen specific immune responses were characterized after intramuscular immunization of BALB/c mice with 5 antigen encoding plasmid DNAs (pDNAs) complexed with Vaxfectin, a cationic lipid formulation. Vaxfectin increased IgG titers for all of the antigens with no effect on the CTL responses to the 2 antigens for which CTL assays ... More
Combined alpha tumor necrosis factor gene therapy and engineered dendritic cell vaccine in combating well-established tumors.
AuthorsLiu Y, Saxena A, Zheng C, Carlsen S, Xiang J
JournalJ Gene Med
PubMed ID15293344
Although current immunotherapeutic strategies including adenovirus (AdV)-mediated gene therapy and dendritic cell (DC) vaccine can all stimulate antitumor cytotoxic T lymphocyte (CLT) responses, their therapeutic efficiency has still been limited to generation of prophylactic antitumor immunity against re-challenge with the parental tumor cells or growth inhibition of small tumors in ... More
B7/CD28 costimulation is essential for the homeostasis of the CD4+CD25+ immunoregulatory T cells that control autoimmune diabetes.
AuthorsSalomon B, Lenschow DJ, Rhee L, Ashourian N, Singh B, Sharpe A, Bluestone JA
JournalImmunity
PubMed ID10795741
CD28/B7 costimulation has been implicated in the induction and progression of autoimmune diseases. Experimentally induced models of autoimmunity have been shown to be prevented or reduced in intensity in mice rendered deficient for CD28 costimulation. In sharp contrast, spontaneous diabetes is exacerbated in both B7-1/B7-2-deficient and CD28-deficient NOD mice. These ... More
Evaluation of immunoprophylactic efficacy of Brugia malayi transglutaminase (BmTGA) in single and multiple antigen vaccination with BmALT-2 and BmTPX for human lymphatic filariasis.
An attempt was made to study the immunoprophylactic efficacy of recombinant Brugia malayi transglutaminase (BmTGA) as protein vaccine along with two other recombinant proteins, Brugia malayi abundant larval transcript-2 (BmALT-2) and Brugia malayi thioredoxin peroxidase (BmTPX), in single and multiple antigen form for human lymphatic filariasis. Parasite challenge studies in ... More
'Educated' dendritic cells act as messengers from memory to naive T helper cells.
AuthorsAlpan O, Bachelder E, Isil E, Arnheiter H, Matzinger P
JournalNat Immunol
PubMed ID15156140
Ingested antigens lead to the generation of effector T cells that secrete interleukin 4 (IL-4) rather than interferon-gamma (IFN-gamma) and are capable of influencing naive T cells in their immediate environment to do the same. Using chimeric mice generated by aggregation of two genotypically different embryos, we found that the ... More
Immunization by Arg-gingipain A DNA vaccine protects mice against an invasive Porphyromonas gingivalis infection through regulation of interferon-gamma production.
AuthorsYonezawa H, Kato T, Kuramitsu HK, Okuda K, Ishihara K
JournalOral Microbiol Immunol
PubMed ID16101960
We previously demonstrated that a Porphyromonas gingivalis rgpA DNA vaccine induced protective immune responses against P. gingivalis infection in mice. In the present study, reduction in lethality against infection by lethal doses of P. gingivalis was observed in the rgpA DNA vaccine-immunized mice. Cytokine levels in the mouse model with ... More
Protection against collagen-induced arthritis by electrotransfer of an expression plasmid for the interleukin-4.
AuthorsHo SH, Hahn W, Lee HJ, Kim DS, Jeong JG, Kim S, Yu SS, Jeon ES, Kim S, Kim JM
JournalBiochem Biophys Res Commun
PubMed ID15358092
Rheumatoid arthritis (RA) is a chronic inflammatory joint disease, leading to cartilage and bone destruction. We investigated whether the electrotransfer of IL-4 DNA could regulate the disease progress of murine collagen-induced arthritis (CIA). The maximum serum level of mIL-4 was measured by 340 pg/ml on day 1 following DNA transfer. ... More
Transplanted MHC class I-deficient nonobese diabetic mouse islets are protected from autoimmune injury in diabetic nonobese recipients.
AuthorsPrange S, Zucker P, Jevnikar AM, Singh B
JournalTransplantation
PubMed ID11349735
The injury of transplanted islets may occur by both autoimmune and alloimmune processes directed against MHC targets. To examine the role of MHC class I in islet graft injury, we transplanted syngeneic and allogeneic beta2-microglobulin-deficient islets into diabetic nonobese diabetic (NOD) mice. Loss of graft function was observed within 14 ... More
Cell-based gene therapy modifies matrix remodeling after a myocardial infarction in tissue inhibitor of matrix metalloproteinase-3-deficient mice.
AuthorsAngoulvant D, Fazel S, Weisel RD, Lai TY, Fedak PW, Chen L, Rafati S, Seneviratne CK, Degousee N, Li RK
JournalJ Thorac Cardiovasc Surg
PubMed ID19185172
Cell-based gene therapy can enhance the effects of cell transplantation by temporally and spatially regulating the release of the gene product. The purpose of this study was to evaluate transient matrix metalloproteinase inhibition by implanting cells genetically modified to overexpress a natural tissue inhibitor of matrix metalloproteinases (tissue inhibitor of ... More
Mucosal T cells expressing high levels of IL-7 receptor are potential targets for treatment of chronic colitis.
AuthorsYamazaki M, Yajima T, Tanabe M, Fukui K, Okada E, Okamoto R, Oshima S, Nakamura T, Kanai T, Uehira M, Takeuchi T, Ishikawa H, Hibi T, Watanabe M
JournalJ Immunol
PubMed ID12874249
The IL-7/IL-7R-dependent signaling pathway plays a crucial role in regulating the immune response in intestinal mucosa. Here we demonstrate the pivotal role of this pathway in the development and treatment of chronic colitis. T cells expressing high levels of IL-7R were substantially infiltrated in the chronic inflamed mucosa of TCR ... More
Pertussis toxin sensitization alters the pathogenesis of subsequent respiratory syncytial virus infection.
AuthorsFischer JE, Johnson JE, Johnson TR, Graham BS
JournalJ Infect Dis
PubMed ID10979896
Evidence suggests that both host and viral factors influence disease severity after infection with respiratory syncytial virus (RSV). To characterize the effects of pertussis toxin (PT) sensitization on subsequent RSV infection, BALB/c mice were treated with PT parenterally before RSV challenge. Priming with purified and detoxified PT before RSV challenge ... More
T cell studies in a peptide-induced model of systemic lupus erythematosus.
AuthorsKhalil M, Inaba K, Steinman R, Ravetch J, Diamond B
JournalJ Immunol
PubMed ID11160209
We have previously reported that immunization with a peptide mimetope of dsDNA on a branched polylysine backbone (DWEYSVWLSN-MAP) induces a systemic lupus erythematosus-like syndrome in the nonautoimmune BALB/c mouse strain. To understand the mechanism underlying this breakdown in self tolerance, we examined the role of T cells in the response. ... More
Protective immunity induced by the full-length cDNA encoding paramyosin of Chinese Schistosoma japonicum.
AuthorsZhou S, Liu S, Song G, Xu Y, Sun W
JournalVaccine
PubMed ID10856799
The full-length cDNA encoding paramyosin of Chinese S. japonicum (Sjc97) has been cloned and sequenced for the first time. The homology of the nucleotide sequence of paramyosin and the deduced amino acid sequence between Chinese, Philippine, Japanese strains and S. mansoni (Sm97) were 99.7, 99.8 and 96% at amino acid ... More
Allergic diathesis in transgenic mice with constitutive T cell expression of inducible vasoactive intestinal peptide receptor.
AuthorsVoice JK, Dorsam G, Lee H, Kong Y, Goetzl EJ
JournalFASEB J
PubMed ID11689474
Vasoactive intestinal peptide (VIP) and its G-protein-coupled receptors (VPAC1 and VPAC2 Rs) are prominent in the immune system. In T cells, VPAC1 R is expressed constitutively whereas VPAC2 R is induced only after stimulation of the T cell receptor (TCR) or exposure to some cytokines. VPAC1 R and VPAC2 R ... More
CXCL10 (IFN-gamma-inducible protein-10) control of encephalitogenic CD4+ T cell accumulation in the central nervous system during experimental autoimmune encephalomyelitis.
Experimental autoimmune encephalomyelitis (EAE) is a CD4(+) Th1-mediated demyelinating disease of the CNS that serves as a model for multiple sclerosis. A critical event in the pathogenesis of EAE is the entry of both Ag-specific and Ag-nonspecific T lymphocytes into the CNS. In the present report, we investigated the role ... More
Enhancement of primary and secondary cellular immune responses against human immunodeficiency virus type 1 gag by using DNA expression vectors that target Gag antigen to the secretory pathway.
AuthorsQiu JT, Liu B, Tian C, Pavlakis GN, Yu XF
JournalJ Virol
PubMed ID10846081
In this study, we have investigated the influence of antigen targeting after DNA vaccination upon the induction of cellular immune responses against human immunodeficiency virus type 1 (HIV-1) Gag. In addition to the standard version of HIV-1 Gag, we constructed Gag expression vectors that encode a secreted (Sc-Gag) and a ... More
CD4+Thy1- thymocytes with a Th-type 2 cytokine response.
AuthorsCerasoli DM, Kelsoe G, Sarzotti M
JournalInt Immunol
PubMed ID11133836
We have identified a small subset of CD3(+), CD4(+), CD8(-) thymocytes that do not express Thy1 (CD90). This Thy1(-) subset represents 1-3.7% of the total number of thymocytes in a naive mouse. CD4(+)Thy1(-) thymocytes express high levels of CD3, intermediate to high levels of heat-stable antigen (HSA), and low levels ... More
Blockade of transforming growth factor beta/Smad signaling in T cells by overexpression of Smad7 enhances antigen-induced airway inflammation and airway reactivity.
AuthorsNakao A, Miike S, Hatano M, Okumura K, Tokuhisa T, Ra C, Iwamoto I
JournalJ Exp Med
PubMed ID10899902
Transforming growth factor (TGF)-beta has been implicated in immunosuppression. However, it remains obscure whether regulation of T cells by TGF-beta contributes to the immunosuppression in vivo. To address this issue, we developed transgenic mice expressing Smad7, an intracellular antagonist of TGF-beta/Smad signaling, selectively in mature T cells using a plasmid ... More
Epitope-specific antibody response to Mel-CAM induced by mimotope immunization.
AuthorsHafner C, Wagner S, Jasinska J, Allwardt D, Scheiner O, Wolff K, Pehamberger H, Wiedermann U, Breiteneder H
JournalJ Invest Dermatol
PubMed ID15654965
Peptide mimotopes of tumor antigen epitopes have been proposed as components of tumor vaccines. In this study, we determined the immunogenicity of melcam mim1 and melcam mim2, peptide mimics of an epitope of the melanoma cell-adhesion molecule (Mel-CAM). BALB/c mice were vaccinated either with mimotopes or mimotopes coupled to tetanus ... More
High-level expression of interleukin-4 following electroporation-mediated gene transfer accelerates Type 1 diabetes in NOD mice.
AuthorsHoriki M, Yamato E, Noso S, Ikegami H, Ogihara T, Miyazaki J
JournalJ Autoimmun
PubMed ID12657524
Nonobese diabetic (NOD) mice develop T cell-dependent autoimmune disease. Administration of interleukin-4 (IL-4), one of the T helper 2 (Th2) cytokines, is reported to prevent either insulitis or diabetes or both in NOD mice. We examined the effect of transferring an IL-4-expressing plasmid vector into muscle by in vivo electroporation ... More
Prevention of lethal acute graft-versus-host disease in mice by oral administration of T helper 1 inhibitor, TAK-603.
AuthorsLu Y, Sakamaki S, Kuroda H, Kusakabe T, Konuma Y, Akiyama T, Fujimi A, Takemoto N, Nishiie K, Matsunaga T, Hirayama Y, Kato J, Kon S, Kogawa K, Niitsu Y
JournalBlood
PubMed ID11159546
Acute graft-versus-host diseases (GVHD) is a major cause of morbidity and mortality in patients undergoing allogeneic bone marrow transplantation (BMT). T helper 1 (Th1)-type cytokines such as interferon-gamma or tumor necrosis factor-alpha have been implicated in the pathogenesis of acute GVHD. TAK-603 is a new quinoline derivative, which is now ... More
Evidence of GATA-3-dependent Th2 commitment during the in vivo immune response.
AuthorsTamauchi H, Terashima M, Ito M, Maruyama H, Ikewaki N, Inoue M, Gao X, Hozumi K, Habu S
JournalInt Immunol
PubMed ID14688073
The transcription factor GATA-3 has been shown to play an important role for the in vitro induction of T(h)2 cells. To clarify how the in vivo immune response is governed under GATA-3 function, we generated double-transgenic mice by crossing GATA-3 transgenic mice with ovalbumin (OVA)-specific TCR transgenic mice. After immunization ... More
The immunomodulating properties of human respiratory syncytial virus and immunostimulating complexes containing Quillaja saponin components QH-A, QH-C and ISCOPREP703.
AuthorsHu KF, Regner M, Siegrist CA, Lambert P, Chen M, Bengtsson KL, Morein B
JournalFEMS Immunol Med Microbiol
PubMed ID15681158
A successful vaccine against human RSV (HRSV) is likely to induce a Th1 or a balanced Th1/TH2 cytokine response. We tested a panel of HRSV immunostimulating complexes (ISCOMs) containing different Quillaja saponin fractions (QH-A, QH-C, and 703: a mixture of 70% QH-A and 30% QH-C) with different immunological properties for ... More
Ragweed-induced expression of GATA-3, IL-4, and IL-5 by eosinophils in the lungs of allergic C57BL/6J mice.
AuthorsJustice JP, Borchers MT, Lee JJ, Rowan WH, Shibata Y, Van Scott MR
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID11792635
Allergen-induced recruitment of T lymphocytes and eosinophils to the airways is associated with increased expression of the transcription factor GATA-3. In this study, the relationship between airway inflammation and GATA-3 expression in the lungs was investigated using ragweed-sensitized C57BL/6J mice. Intratracheal ragweed challenge increased both the number of GATA-3-expressing cells ... More
Complex role of the IL-4 receptor alpha in a murine model of airway inflammation: expression of the IL-4 receptor alpha on nonlymphoid cells of bone marrow origin contributes to severity of inflammation.
AuthorsKelly-Welch AE, Melo ME, Smith E, Ford AQ, Haudenschild C, Noben-Trauth N, Keegan AD
JournalJ Immunol
PubMed ID15034072
Recent studies have suggested the IL-4Ralpha expressed on lung epithelium is necessary for TH2-mediated goblet cell differentiation and mucus hypersecretion in a murine model of allergic lung disease. However, the IL-4Ralpha is expressed on numerous cell types that could contribute to the overall pathology and severity of asthma. The relative ... More
Murine keratocytes function as antigen-presenting cells.
AuthorsSeo SK, Gebhardt BM, Lim HY, Kang SW, Higaki S, Varnell ED, Hill JM, Kaufman HE, Kwon BS
JournalEur J Immunol
PubMed ID11745349
Keratocytes express MHC class I molecules constitutively, and keratocytes stimulated with IFN-gamma express MHC class II molecules. Unstimulated keratocytes constitutively express B7-1 and ICAM-1, as well as low levels of CD40 and 4-1BBL. These findings indicate that keratocytes may deliver both antigen-specific and costimulatory signals to CD4(+) and CD8(+) T ... More
The key regulators of adult T helper cell responses, STAT6 and T-bet, are established in early life in mice.
AuthorsRose S, Guevara P, Farach S, Adkins B
JournalEur J Immunol
PubMed ID16568497
Murine neonatal immunity is typically Th2 biased. This is characterized by high-level IL-4 production at all phases of the immune response and poor IFN-gamma memory responses. The differential expression of Th1/Th2 cytokines by neonates and adults could arise if the critical regulators of Th differentiation and function, STAT6 and T-bet, ... More
Time course of inflammatory and remodeling events in a murine model of asthma: effect of steroid treatment.
AuthorsTrifilieff A, El-Hashim A, Bertrand C
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID11076802
The kinetics of airway inflammation and remodeling processes following ovalbumin aerosol challenge in sensitized BALB/c mice was studied. Mice were exposed to either single or five ovalbumin challenges over 5 days. In both protocols, time-dependent increases in bronchoalveolar lavage (BAL) cellular fibronectin, neutrophils and eosinophils were observed. The kinetics of ... More
Migration-inhibitory factor gene-deficient mice are susceptible to cutaneous Leishmania major infection.
AuthorsSatoskar AR, Bozza M, Rodriguez Sosa M, Lin G, David JR
JournalInfect Immun
PubMed ID11159984
To determine the role of endogenous migration-inhibitory factor (MIF) in the development of protective immunity against cutaneous leishmaniasis, we analyzed the course of cutaneous Leishmania major infection in MIF gene-deficient mice (MIF(-/-)) and wild-type (MIF(+/+)) mice. Following cutaneous L. major infection, MIF(-/-) mice were susceptible to disease and developed significantly ... More
Footpad reaction induced by Neospora caninum tachyzoite extract in infected BALB/c mice.
AuthorsOmata Y, Kamiya H, Kano R, Kobayashi Y, Maeda R, Saito A
JournalVet Parasitol
PubMed ID16616423
Little information is available regarding a delayed type hypersensitivity (DTH) reaction in neosporosis. In this study, we examined the elicitation of a DTH reaction in mice infected with Neospora caninum by inoculation of the footpad with tachyzoite antigens. The footpads of BALB/c mice infected with N. caninum and those of ... More
Effect of a single oral dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin on immune function in male NC/Nga mice.
AuthorsFujimaki H, Nohara K, Kobayashi T, Suzuki K, Eguchi-Kasai K, Tsukumo S, Kijima M, Tohyama C
JournalToxicol Sci
PubMed ID11861978
Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces immunosuppression in humans and animals. However, the effect of TCDD on Th2-type immune responses such as allergic reactions has been unclear. Using NC/Nga mice that developed atopic dermatitis-like skin lesions with marked elevation in plasma of total IgE when bred under conventional conditions, we investigated ... More
IL-1 is required for allergen-specific Th2 cell activation and the development of airway hypersensitivity response.
AuthorsNakae S, Komiyama Y, Yokoyama H, Nambu A, Umeda M, Iwase M, Homma I, Sudo K, Horai R, Asano M, Iwakura Y
JournalInt Immunol
PubMed ID12663678
IL-1 is a pro-inflammatory cytokine consisted of two molecular species, IL-1alpha and IL-1beta, and the IL-1 receptor antagonist (IL-1Ra) is a natural inhibitor of both molecules. Although it is suggested that IL-1 potentiates immune responses mediated by T(h)2 cells, the role of IL-1 in asthma still remains unclear. In this ... More
Insulin-induced remission in new-onset NOD mice is maintained by the PD-1-PD-L1 pathway.
AuthorsFife BT, Guleria I, Gubbels Bupp M, Eagar TN, Tang Q, Bour-Jordan H, Yagita H, Azuma M, Sayegh MH, Bluestone JA
JournalJ Exp Med
PubMed ID17116737
The past decade has seen a significant increase in the number of potentially tolerogenic therapies for treatment of new-onset diabetes. However, most treatments are antigen nonspecific, and the mechanism for the maintenance of long-term tolerance remains unclear. In this study, we developed an antigen-specific therapy, insulin-coupled antigen-presenting cells, to treat ... More
Effect of orally administered rolipram, a phosphodiesterase 4 inhibitor, on a mouse model of the dermatitis caused by 2,4,6-trinitro-1-chlorobenzene (TNCB)-repeated application.
AuthorsHarada D, Tsukumo Y, Takashima Y, Manabe H
JournalEur J Pharmacol
PubMed ID16442096
The purpose of this study was to evaluate the efficacy of rolipram, a phosphodiesterase (PDE) 4 inhibitor, in a mouse model of dermatitis induced by repeated application of 2,4,6-trinitro-1-chlorobenzene (TNCB). BALB/c mice were sensitized with 0.3% w/v TNCB applied to the ear on day -7, followed by application three times ... More
Suppression of CD4+ T cell activation by a novel inhibitor of Src family kinases.
AuthorsMcRae BL, Wallace C, Dixon KF, Roux A, Mohan S, Jia Y, Presky DH, Tracey DE, Hirst GC
JournalInt Immunopharmacol
PubMed ID15710336
The Src family kinases Lck and Fyn play an important role in T cell development and function. We have synthesized a novel small molecule, A-420983, which inhibits Lck and Fyn, as well as other Src family kinases, but has selectivity with respect to non-Src family kinases. A-420983 completely inhibited antigen-stimulated ... More
Abrogation of lung inflammation in sensitized Stat6-deficient mice is dependent on the allergen inhalation procedure.
AuthorsTrifilieff A, El-Hasim A, Corteling R, Owen CE
JournalBr J Pharmacol
PubMed ID10928961
Conflicting results have been reported about the role of Stat6 in allergen-induced airway inflammation. We have studied the influence of the allergen inhalation procedure on the inflammatory response using wild-type and Stat6-deficient mice generated on a C57BL/6 background. Animals were immunized i.p. on day 0 and 7 with ovalbumin (OVA) ... More