NO-aspirin protects from T cell-mediated liver injury by inhibiting caspase-dependent processing of Th1-like cytokines.
AuthorsFiorucci S, Santucci L, Antonelli E, Distrutti E, Del Sero G, Morelli O, Romani L, Federici B, Del Soldato P, Morelli A
JournalGastroenterology
PubMed ID10648469
Concanavalin A (con A)-induced hepatitis is an immunomediated disease in which assembly of CD4(+) T cells and T helper (Th)1-like cytokines causes Fas-mediated liver cell death. Nitric oxide (NO) modulates Th1 response in vitro. NCX-4016 is an NO-aspirin derivative that spares the gastrointestinal tract and shares molecular targets with NO. ... More
Cytidine-phosphate-guanosine oligodeoxynucleotides and interferon-alpha-expressing tumor cells effectively induce dendritic cell maturation in vitro.
AuthorsHiraide A, Hiroishi K, Ishii S, Eguchi J, Imawari M
JournalAnticancer Res
PubMed ID16475701
'Dendritic cells (DCs) play an important role in immune response and cytidine-phosphate-guanosine (CpG) oligodeoxynucleotides (ODN) as well as interferon (IFN)-alpha have been proven to induce DC maturation. In this study, the synergistic effects of CpG-ODN and IFN-alpha on DC maturation were evaluated.' ... More
Changes in serum levels of cytokines in mice injected with an immunostimulator C3bgp isolated from Cuscuta europea.
AuthorsStanilova S, Dobreva Z, Zhelev Z
JournalInt Immunopharmacol
PubMed ID11515822
'The effect of C3 binding glycoprotein (C3bgp), isolated from Cuscuta europea seeds on induction of in vivo cytokine synthesis was investigated. Different groups of mice were stimulated with 30 microg C3bgp per mouse, injected intraperitoneally. The quantitative determination of IL-1alpha, IL-2, IL-4, IL-6, IL-10, TNF-alpha and IFN-gamma was performed in ... More
Lipopolysaccharide-induced cytokine and receptor expression and neutrophil infiltration in the liver of osteopetrosis (op/op) mutant mice.
AuthorsJiang S, Naito M, Kaizu C, Kuwata K, Hasegawa G, Mukaida N, Shultz LD
JournalLiver
PubMed ID11169061
'Mice homozygous for the osteopetrosis (op) mutation are genetically deficient in macrophage colony-stimulating factor (M-CSF/CSF-1) and are characterized by defective differentiation and function of macrophages. The aim of this study is to assess the contribution of M-CSF to lipopolysaccharide (LPS)-induced cytokine expression and neutrophil infiltration in the liver.' ... More
Implication of STAT1 and STAT3 transcription factors in the response to superantigens.
AuthorsPlaza R, Vidal S, Rodriguez-Sanchez JL, Juarez C
JournalCytokine
PubMed ID14687580
'The activation of STAT1 and STAT3 in response to SEB was analyzed in spleen of Balb/c mice. The intraperitoneal injection of the superantigen SEB activated STAT1 and STAT3 in spleen. Activated STAT1 almost completely disappeared in 24 h even though activated STAT3 was present for more than 48 h after ... More
Redundant and alternative roles for activating Fc receptors and complement in an antibody-dependent model of autoimmune vitiligo.
AuthorsTrcka J, Moroi Y, Clynes RA, Goldberg SM, Bergtold A, Perales MA, Ma M, Ferrone CR, Carroll MC, Ravetch JV, Houghton AN
JournalImmunity
PubMed ID12121667
'Complement and Fc receptor (FcR)-positive cells mediate effector functions of antibodies. Antibody-dependent immunity against the melanosome membrane glycoprotein gp75/tyrosinase-related protein-1 (TYRP-1) of melanocytes leads to autoimmune hypopigmentation (vitiligo) in mice. Hypopigmentation occurred in mice deficient in activating FcR containing the common gamma subunit (Fc gamma R gamma(-/-)) and in mice ... More
Intensification of antitumor effect by T helper 1-dominant adoptive immunogene therapy for advanced orthotopic colon cancer.
AuthorsNakamori M, Iwahashi M, Nakamura M, Ueda K, Zhang X, Yamaue H
JournalClin Cancer Res
PubMed ID12796406
'T helper (Th) 1/Th2 balance, controlled by Th1 or Th2 cells producing cytokines, plays important roles in antitumor immunity. Interleukin-18 (IL-18) can act together with IL-12 in promoting the generation of IFN-gamma producing Th1 cells. The goal of this study was to determine whether cytotoxic T lymphocyte (CTL) secreted in ... More
Inhibition of phosphoinositide 3-kinase delta attenuates allergic airway inflammation and hyperresponsiveness in murine asthma model.
AuthorsLee KS, Lee HK, Hayflick JS, Lee YC, Puri KD
JournalFASEB J
PubMed ID16507763
'P110delta phosphoinositide 3-kinase (PI3K) plays a pivotal role in the recruitment and activation of certain inflammatory cells. Recent findings revealed that the activity of p110delta also contributes to allergen-IgE-induced mast cell activation and vascular permeability. We investigated the role of p110delta in allergic airway inflammation and hyperresponsiveness using IC87114, a ... More
B-cell-deficient mice show an exacerbated inflammatory response in a model of Chlamydophila abortus infection.
AuthorsBuendía AJ, Del Río L, Ortega N, Sánchez J, Gallego MC, Caro MR, Navarro JA, Cuello F, Salinas J
JournalInfect Immun
PubMed ID12438369
'The resolution of Chlamydophila abortus (Chlamydia psittaci serotype 1) infection is dependent on gamma interferon and CD8(+) T cells, and classically, B cells have been considered to play a minimal role in host defense. The role of B cells in the immune response was studied by using a model of ... More
Glossogyne tenuifolia acts to inhibit inflammatory mediator production in a macrophage cell line by downregulating LPS-induced NF-kappa B.
AuthorsWu MJ, Wang L, Ding HY, Weng CY, Yen JH
JournalJ Biomed Sci
PubMed ID14966369
'Glossogyne tenuifolia (hsiang-ju) (GT) is a traditional antipyretic herb used in Chinese medicine; however, no information is available to explain its action. The objective of this research was to elucidate the molecular pharmacological activity and the effective components in the ethanol extract of GT. We found that GT had potent ... More
Anti-inflammatory properties of piperlactam S: modulation of complement 5a-induced chemotaxis and inflammatory cytokines production in macrophages.
AuthorsChiou WF, Peng CH, Chen CF, Chou CJ
JournalPlanta Med
PubMed ID12567272
'Macrophages infiltrate tissues in response to chemoattractants including complement 5a (C5a). Infiltrating macrophages clear microorganisms but also can cause tissue damage. We hypothesized that prevention of macrophages from excessive recruitment into infected sites may underlie the anti-inflammatory effects of piperlactam S, an alkaloid isolated from Piper kadsura (Choisy) Ohwi. To ... More
Involvement of ERK, p38 and NF-kappaB signal transduction in regulation of TLR2, TLR4 and TLR9 gene expression induced by lipopolysaccharide in mouse dendritic cells.
AuthorsAn H, Yu Y, Zhang M, Xu H, Qi R, Yan X, Liu S, Wang W, Guo Z, Guo J, Qin Z, Cao X
JournalImmunology
PubMed ID11972630
'Toll-like receptors (TLR) are sentinel receptors capable of recognizing pathogen-associated molecule patterns (PAMP) such as lipopolysaccharide (LPS) and CpG-containing oligonucleotides (CpG ODN). TLR2 and TLR4 are major receptors for Gram-positive and Gram-negative bacterial cell wall components, respectively. TLR9 is necessary for CpG signalling. LPS or CpG ODN can activate immature ... More
Retroviral gene therapy with an immunoglobulin-antigen fusion construct protects from experimental autoimmune uveitis.
AuthorsAgarwal RK, Kang Y, Zambidis E, Scott DW, Chan CC, Caspi RR
JournalJ Clin Invest
PubMed ID10903340
'Immunoglobulins can serve as tolerogenic carriers for antigens, and B cells can function as tolerogenic antigen-presenting cells. We used this principle to design a strategy for gene therapy of experimental autoimmune uveitis, a cell-mediated autoimmune disease model for human uveitis induced with the uveitogenic interphotoreceptor retinoid-binding protein (IRBP). A retroviral ... More
Acute hepatotoxicity of oncolytic adenoviruses in mouse models is associated with expression of wild-type E1a and induction of TNF-alpha.
AuthorsEngler H, Machemer T, Philopena J, Wen SF, Quijano E, Ramachandra M, Tsai V, Ralston R
JournalVirology
PubMed ID15380358
'Replication competent adenoviruses with various E1 modifications designed to restrict their replication to tumor cells are being evaluated as oncolytic agents in clinical trials. In mouse models, we observed that such oncolytic adenoviruses showed greater hepatotoxicity than E1-deleted adenovirus vectors following intravenous administration. Additional studies in congenic BALB/c, nude, and ... More
Determinants of autoantibody induction by conjugated papillomavirus virus-like particles.
AuthorsChackerian B, Lenz P, Lowy DR, Schiller JT
JournalJ Immunol
PubMed ID12444114
'Immunization of mice with self-Ag arrayed on the surface of papillomavirus-like particles induces long-lasting high-titer IgG production by autoreactive B cells. In contrast, immunization with disorganized self-Ag linked to foreign Th epitopes induces weak autoantibody responses that are predominantly of the IgM isotype. In this study, we evaluated the structural ... More
In vitro study of the immune stimulating activity of an atrophic [correction of athrophic] rhinitis vaccine associated to chitosan microspheres.
AuthorsJiang HL, Park IK, Shin NR, Kang SG, Yoo HS, Kim SI, Suh SB, Akaike T, Cho CS
JournalEur J Pharm Biopharm
PubMed ID15451520
'Chitosan microspheres (CMs) were prepared by an ionic gelation process with tripolyphosphate and characterized. Bordetella Bronchiseptica Dermonecrotoxin (BBD), a major virulence factor of a causative agent of atrophic rhinitis (AR), was loaded on to the CMs for nasal vaccination. BBD-loaded CMs were observed as aggregated shapes although unloaded CMs were ... More
Brucella abortus bacA mutant induces greater pro-inflammatory cytokines than the wild-type parent strain.
AuthorsParent MA, Goenka R, Murphy E, Levier K, Carreiro N, Golding B, Ferguson G, Roop RM, Walker GC, Baldwin CL
JournalMicrobes Infect
PubMed ID17196866
'The inner-membrane protein BacA affects Brucella LPS structure. A bacA deletion mutant of Brucella abortus, known as KL7 (bacA(mut)-KL7), is attenuated in BALB/c mice and protects against challenge. Thus, bacA mutation was a candidate for incorporation into live attenuated vaccines. We assessed bacA(mut)-KL7 in 2 additional mouse strains: the more ... More
Chronic cerebral hypoperfusion induced by right unilateral common carotid artery occlusion causes delayed white matter lesions and cognitive impairment in adult mice.
AuthorsYoshizaki K, Adachi K, Kataoka S, Watanabe A, Tabira T, Takahashi K, Wakita H
JournalExp Neurol
PubMed ID18222425
'Some lines of evidence have suggested that subcortical ischemic vascular dementia (SIVD) is a common form of vascular dementia (VaD), and that its pathological changes are the development of ischemic white matter (WM) lesions under chronic hypoperfusion and lacunes. Here, we have developed a novel mouse model of VaD with ... More
Overexpression of interleukin-4 in lungs of mice impairs elimination of Histoplasma capsulatum.
'Protection against the pathogenic fungus Histoplasma capsulatum requires Th1 cytokines. Since interleukin-4 (IL-4) can inhibit both Th1 cytokine production and activity, we examined the effects of overproduction of IL-4 in the lung on the course of pulmonary histoplasmosis. IL-4 lung transgenic mice manifested a higher fungal burden in their lungs, ... More
Downregulation of programmed cell death 4 by inflammatory conditions contributes to the generation of the tumor promoting microenvironment.
AuthorsYasuda M, Schmid T, Rübsamen D, Colburn NH, Irie K, Murakami A
JournalMol Carcinog
PubMed ID20607724
'Ample evidence has shown key roles of inflammation in tumor promotion and carcinogenesis, and tumor-associated macrophages are known to promote tumor growth and dissemination. Programmed cell death 4 (Pdcd4) is a novel tumor suppressor, and although various studies have revealed that the functions and expression mechanisms of Pdcd4 in tumor ... More
Preexposure of murine macrophages to CpG oligonucleotide results in a biphasic tumor necrosis factor alpha response to subsequent lipopolysaccharide challenge.
AuthorsCrabtree TD, Jin L, Raymond DP, Pelletier SJ, Houlgrave CW, Gleason TG, Pruett TL, Sawyer RG
JournalInfect Immun
PubMed ID11254566
'Bacterial DNA and synthetic oligonucleotides containing CpG sequences (CpG-DNA and CpG-ODN) provoke a proinflammatory cytokine response (tumor necrosis factor alpha [TNF-alpha], interleukin-12 [IL-12], and IL-6) and increased mortality in lipopolysaccharide (LPS)-challenged mice via a TNF-alpha-mediated mechanism. It was hypothesized that preexposure of macrophages to CpG-ODN would result in an increased ... More
Several functions of immune cells in mice changed by oxidative stress caused by endotoxin.
AuthorsVíctor VM, De la Fuente M
JournalPhysiol Res
PubMed ID14640902
'We have studied natural killer (NK) activity, lymphoproliferative response, the release of several cytokines (IL-2, TNF alpha and IL-1 beta) and the ROS production in peritoneal leukocytes obtained 0, 2, 4, 12 and 24 h after lipopolysaccharide (LPS) injection. Lethal septic shock (100 % mortality occurred at 30 h after ... More
Biphasic Effect of Phyllanthus emblica L. Extract on NSAID-Induced Ulcer: An Antioxidative Trail Weaved with Immunomodulatory Effect.
AuthorsChatterjee A, Chattopadhyay S, Bandyopadhyay SK
JournalEvid Based Complement Alternat Med
PubMed ID21076542
'Amla (Phyllanthus emblica L.), apart from its food value, can be used as a gastroprotective agent in non steroidal anti-inflammatory drug (NSAID)-induced gastropathy. It has been suggested that the antioxidative property of amla is the key to its therapeutic effect. Hence, on the basis of in vitro antioxidative potential, the ... More
High-yield isolation of murine microglia by mild trypsinization.
AuthorsSaura J, Tusell JM, Serratosa J
JournalGlia
PubMed ID14603460
'Microglia can be isolated with high purity but low yield by shaking off loosely adherent cells from mixed glial cultures. Here we describe a new technique for isolating microglia with an average yield close to 2,000,000 microglial cells/mouse pup, more than five times higher than that of the shaking method. ... More
Passive serum therapy with polyclonal antibodies against Mycobacterium tuberculosis protects against post-chemotherapy relapse of tuberculosis infection in SCID mice.
AuthorsGuirado E, Amat I, Gil O, Díaz J, Arcos V, Caceres N, Ausina V, Cardona PJ
JournalMicrobes Infect
PubMed ID16702016
'We investigated the protective role of immune-sera against reactivation of Mycobacterium tuberculosis infection in SCID mice and found that passive immunization with sera obtained from mice treated with detoxified M. tuberculosis extracts (delivered in liposomes in a composition known as RUTI) exerted significant protection. Our SCID mouse model consisted of ... More
Immunoglobulin and cytokine production from spleen lymphocytes is modulated in C57BL/6J mice by dietary cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid.
AuthorsYamasaki M, Chujo H, Hirao A, Koyanagi N, Okamoto T, Tojo N, Oishi A, Iwata T, Yamauchi-Sato Y, Yamamoto T, Tsutsumi K, Tachibana H, Yamada K
JournalJ Nutr
PubMed ID12612153
'We evaluated the effect of cis-9, trans-11 (9c,11t) and trans-10, cis-12 (10t,12c) conjugated linoleic acid (CLA) on the immune system in C57BL/6J mice. Mice were fed experimental diets containing 0% CLA (controls), 1% 9c,11t-CLA, 1% 10t,12c-CLA or a 1:1 mixture (0.5% + 0.5%) of these two CLA isomers for 3 ... More
I kappa B overexpression in cardiomyocytes prevents NF-kappa B translocation and provides cardioprotection in trauma.
'This study examined the effects of either IkappaBalpha overexpression (transgenic mice) or N-acetyl-leucinyl-leucinyl-norleucinal (ALLN) administration (proteosome inhibitor in wild-type mice) on cardiomyocyte secretion of tumor necrosis factor-alpha (TNF-alpha) and on cardiac performance after burn trauma. Transgenic mice were divided into four experimental groups. IkappaBalpha overexpressing mice were given a third-degree ... More
Chronic ethanol feeding potentiates Fas Jo2-induced hepatotoxicity: role of CYP2E1 and TNF-alpha and activation of JNK and P38 MAP kinase.
AuthorsWang X, Lu Y, Xie B, Cederbaum AI
JournalFree Radic Biol Med
PubMed ID19477265
'We have previously shown that treatment of mice with pyrazole or acute ethanol potentiated Fas agonistic Jo2 antibody-induced liver injury by a mechanism involving induction of CYP2E1 and elevated oxidative stress. The current study evaluated whether chronic alcohol feeding potentiates Fas-induced liver injury and whether CYP2E1 plays a role in ... More
The potential of mannosylated chitosan microspheres to target macrophage mannose receptors in an adjuvant-delivery system for intranasal immunization.
AuthorsJiang HL, Kang ML, Quan JS, Kang SG, Akaike T, Yoo HS, Cho CS
JournalBiomaterials
PubMed ID18221992
'A vaccine delivery system based on mannosylated chitosan microspheres (MCMs) was studied in vitro and in vivo. Bordetella bronchiseptica antigens containing dermonecrotoxin (BBD) were loaded in MCMs or chitosan microspheres (CMs). Fluorescence confocal microscopy indicated that BBD-loaded MCMs (BBD-MCMs) bound with mannose receptors on murine macrophages (RAW264.7 cells). In vitro ... More
Therapeutic effect of lecithinized superoxide dismutase against colitis.
AuthorsIshihara T, Tanaka K, Tasaka Y, Namba T, Suzuki J, Ishihara T, Okamoto S, Hibi T, Takenaga M, Igarashi R, Sato K, Mizushima Y, Mizushima T
JournalJ Pharmacol Exp Ther
PubMed ID18927353
'Ulcerative colitis (UC) involves intestinal mucosal damage induced by reactive oxygen species (ROS), in particular, superoxide anion. Superoxide dismutase (SOD) catalyzes dismutation of superoxide anion to hydrogen peroxide, which is subsequently detoxified by catalase. Lecithinized SOD (PC-SOD) is a new modified form of SOD that has overcome previous clinical limitations ... More
Selective priming to Toll-like receptor 4 (TLR4), not TLR2, ligands by P. acnes involves up-regulation of MD-2 in mice.
AuthorsRomics L, Dolganiuc A, Kodys K, Drechsler Y, Oak S, Velayudham A, Mandrekar P, Szabo G
JournalHepatology
PubMed ID15349893
'Lipopolysaccharide (LPS) triggers cytokine production through Toll-like receptor 4 (TLR4), which shares downstream signaling pathways with TLR2. We investigated the roles of TLR2 and TLR4 in Propionibacterium acnes (P. acnes)-primed, LPS-induced liver damage using selective TLR ligands. Stock LPS induced interleukin 8 in both TLR4- and TLR2-expressing human embryonic kidney ... More
S-adenosyl-L-methionine decreases the elevated hepatotoxicity induced by Fas agonistic antibody plus acute ethanol pretreatment in mice.
AuthorsWang X, Cederbaum AI
JournalArch Biochem Biophys
PubMed ID18482574
'The current study was designed to investigate the effect and potential mechanism of exogenous administration of S-adenosyl-l-methionine (SAM) on the enhanced hepatotoxicity induced by the Fas agonistic Jo2 antibody plus acute ethanol pretreatment in C57BL/6 mice. Acute ethanol plus Jo2 treatment produces liver toxicity under conditions in which ethanol alone ... More
Inflammatory skin disease in K14/p40 transgenic mice: evidence for interleukin-12-like activities of p40.
'The proinflammatory cytokine interleukin-12, a p35/p40 heterodimer, is produced by resident cells in skin and has been implicated as a pathogenetic factor in T-cell-mediated skin diseases. Secretion of heterodimeric interleukin-12 is always accompanied by production of p40 monomer and p40/p40 homodimer. To investigate the possible in vivo role of p40 ... More
AuthorsSugawara A, Torigoe T, Tamura Y, Kamiguchi K, Nemoto K, Oguro H, Sato N
JournalCell Stress Chaperones
PubMed ID18686015
'Polyamine compound deoxyspergualin (DSG) is a potent immunosuppressive agent that has been applied clinically for protecting graft rejection and treatment of Wegener''s granulomatosis. Though DSG can bind to heat-shock proteins (HSPs) in cells, its mechanism of immunosuppressive action remains unknown. It is widely accepted that extracellular HSPs are capable of ... More
Absence of the cell cycle inhibitor p21Cip1 reduces LPS-induced NO release and activation of the transcription factor NF-kappaB in mixed glial cultures.
AuthorsTusell JM, Saura J, Serratosa J
JournalGlia
PubMed ID15390102
'We have studied possible differences in glial activation between cells from wild-type and p21Cip1-/- mice. We compared the effect of serum mitogenic stimulation on proliferation rate and on the total number of glial cells after 7 days of culture. No differences between wild-type and p21Cip1-/- glial cells were observed. We ... More
Glutamine's protection against sepsis and lung injury is dependent on heat shock protein 70 expression.
AuthorsSingleton KD, Wischmeyer PE
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID17234954
'Glutamine (GLN) has been shown to protect against inflammatory injury and illness in experimental and clinical settings. The mechanism of this protection is unknown; however, laboratory and clinical trial data have indicated a relationship between GLN-mediated protection and enhanced heat shock protein 70 (HSP70) expression. The aim of this study ... More
Regulation of lymphocyte clustering by CD30-mediated ICAM-1 up-regulation.
AuthorsNam SY, Cho KS, Heo YM, Ha JC, Kim YH, Keun Yi H, Han Hwang P, Kim HM, Podack ER
JournalCell Immunol
PubMed ID12473266
'CD30 is expressed transiently on activated B and T lymphocytes and constitutively on several B- and T cell lymphomas. CD30 functions include participation in negative selection of thymocytes, costimulation of activated T cells, isotype switching of B cells, and regulation of the effector activity of cytotoxic lymphocytes. Although CD30 is ... More
Antigen-specific T cell sensitization is impaired in IL-17-deficient mice, causing suppression of allergic cellular and humoral responses.
AuthorsNakae S, Komiyama Y, Nambu A, Sudo K, Iwase M, Homma I, Sekikawa K, Asano M, Iwakura Y
JournalImmunity
PubMed ID12354389
'Interleukin-17 (IL-17) is a proinflammatory cytokine produced by T cells. The involvement of IL-17 in human diseases has been suspected because of its detection in sera from asthmatic patients and synovial fluids from arthritic patients. In this study, we generated IL-17-deficient mice and investigated the role of IL-17 in various ... More
Nutritional route affects ERK phosphorylation and cytokine production in hepatic mononuclear cells.
AuthorsMoriya T, Fukatsu K, Maeshima Y, Ikezawa F, Ueno C, Kang W, Takakayama E, Saitoh D, Mochizuki H, Miyazaki M
JournalAnn Surg
PubMed ID17414615
To clarify the influence of nutritional route on hepatic immunity in a murine model.
Simvastatin attenuates bleomycin-induced pulmonary fibrosis in mice.
AuthorsOu XM, Feng YL, Wen FQ, Huang XY, Xiao J, Wang K, Wang T
JournalChin Med J (Engl)
PubMed ID19080365
Bleomycin-induced fibrosis is extensively used to model aspects of the pathogenesis of interstitial pulmonary fibrosis. This study aimed to determine the benefic effects and mechanisms of simvastatin on bleomycin-induced pulmonary fibrosis in mice. ... More
Oral (1-->3),(1-->4)-beta-D-glucan synergizes with antiganglioside GD2 monoclonal antibody 3F8 in the therapy of neuroblastoma.
AuthorsCheung NK, Modak S
JournalClin Cancer Res
PubMed ID12006541
In vitro beta-glucan can enhance tumor cytotoxicity through iC3b receptors on leukocytes. We tested if (1-->3),(1-->4)-beta-D-glucan (beta-glucan) can synergize with anti-GD2 monoclonal antibody (MoAb) 3F8 (mouse IgG3) in therapy of human neuroblastoma xenografts. ... More
Rat liver ischemia/reperfusion induced proinflammatory mediator and antioxidant expressions analyzed by gene chips and real-time polymerase chain reactions.
AuthorsHsu YC, Chou TY, Chen CF, Wang D, Su CL, Hu RT
JournalTransplant Proc
PubMed ID18790179
Ischemia/reperfusion (I/R) of the rat liver induces injury; however, few studies have investigated gene expressions associated with this phenomenon. In this study, gene chip and real-time polymerase chain reactions (PCR) were used to study the expressions of the proinflammatory mediators and antioxidants after I/R. ... More
Lack of aromatase improves cell-mediated immune response after burn.
AuthorsPlackett TP, Oz OK, Simpson ER, Kovacs EJ
JournalBurns
PubMed ID16750303
Persistent elevation of estrogens after injury or sepsis correlates with increased mortality and a pro-inflammatory state. Given that aromatase is elevated after injury, the enzyme's subsequent conversion of androstenedione and testosterone to estrone and estradiol may be a causative factor for this correlation. ... More
Reperfusion liver injury induces down-regulation of eNOS and up-regulation of iNOS in lung tissues.
AuthorsLin HI, Chou SJ, Wang D, Feng NH, Feng E, Chen CF
JournalTransplant Proc
PubMed ID16980043
Acute lung injury and inflammation can occur after hepatic ischemia/reperfusion (I/R). Little is known regarding the possible role of nitric oxide synthase expression in this complex type of lung injury. ... More
Induction of acute hepatic injury by endotoxin in mice.
AuthorsXie GQ, Jiang JX, Chen YH, Liu DW, Zhu PF, Wang ZG
JournalHepatobiliary Pancreat Dis Int
PubMed ID14607686
To investigate the changes of scavenger receptor (SR) and CD14 in Kupffer cells in endotoxemia in order to uncover the mechanism of the liver to turn a defense organ into effector one in sepsis. ... More
Agonist of peroxisome proliferator-activated receptor-gamma, rosiglitazone, reduces renal injury and dysfunction in a murine sepsis model.
AuthorsLee S, Kim W, Kang KP, Moon SO, Sung MJ, Kim DH, Kim HJ, Park SK
JournalNephrol Dial Transplant
PubMed ID15797891
Agonists of the peroxisome proliferator-activated receptor-gamma may help to regulate inflammation by modulating the production of inflammatory mediators and adhesion molecules. The purpose of this study was to determine the protective effects of rosiglitazone on renal injury in a sepsis model and to explore the mechanism. ... More
Effect of low-dose aspirin on vascular inflammation, plaque stability, and atherogenesis in low-density lipoprotein receptor-deficient mice.
Atherosclerosis is a complex vascular inflammatory disease. Low-dose aspirin is a mainstay in the prevention of vascular complications of atherosclerosis. We wished to determine the effect of low-dose aspirin on vascular inflammation, plaque composition, and atherogenesis in LDL receptor-deficient mice fed a high fat diet. ... More
Levels of certain tumor markers as differential factors between bilharzial and non-biharzial bladder cancer among Egyptian patients.
AuthorsMetwally NS, Ali SA, Mohamed AM, Khaled HM, Ahmed SA
JournalCancer Cell Int
PubMed ID21473769
Bladder cancer is the commonest type of malignant tumors as a result of schistosomaisis which is a major healthy problem in many subtropical developing countries. The aim of this study is to comparatively elucidate the underlying biochemical tumor markers in schistosomal bladder cancer versus non-schistosomal bladder cancer when compared to ... More
Neonatal immune responses to TLR2 stimulation: influence of maternal atopy on Foxp3 and IL-10 expression.
AuthorsSchaub B, Campo M, He H, Perkins D, Gillman MW, Gold DR, Weiss S, Lieberman E, Finn PW
JournalRespir Res
PubMed ID16551363
Maternal atopic background and stimulation of the adaptive immune system with allergen interact in the development of allergic disease. Stimulation of the innate immune system through microbial exposure, such as activation of the innate Toll-like-receptor 2 (TLR2), may reduce the development of allergy in childhood. However, little is known about ... More
Interleukin 16 is up-regulated in Crohn's disease and participates in TNBS colitis in mice.
AuthorsKeates AC, Castagliuolo I, Cruickshank WW, Qiu B, Arseneau KO, Brazer W, Kelly CP
JournalGastroenterology
PubMed ID11040184
Interleukin (IL)-16 is a T lymphocyte- derived cytokine that uses CD4 as its receptor and hence selectively recruits CD4-bearing cells. Infiltrating CD4(+) T cells are a feature of Crohn's disease; however, the role of IL-16 in intestinal inflammation is unknown. The aim of this study was to determine whether IL-16 ... More
Proinflammatory role of inducible nitric oxide synthase in acute hyperoxic lung injury.
AuthorsHesse AK, Dörger M, Kupatt C, Krombach F
JournalRespir Res
PubMed ID15377396
Hyperoxic exposures are often found in clinical settings of respiratory insufficient patients, although oxygen therapy (>50% O2) can result in the development of acute hyperoxic lung injury within a few days. Upon hyperoxic exposure, the inducible nitric oxide synthase (iNOS) is activated by a variety of proinflammatory cytokines both in ... More
AuthorsHarrison CM, Pompilius M, Pinkerton KE, Ballinger SW
JournalEnviron Health Perspect
PubMed ID21169125
Oxidative stress associated with cardiovascular disease (CVD) risk factors contributes to disease development. However, less is known whether specific subcellular components play a role in disease susceptibility. In this regard, it has been previously reported that vascular mitochondrial damage and dysfunction are associated with atherosclerosis. However, no studies have determined ... More
Complement component c5a is integral to the febrile response of mice to lipopolysaccharide.
AuthorsLi S, Boackle SA, Holers VM, Lambris JD, Blatteis CM
JournalNeuroimmunomodulation
PubMed ID15785108
The complement system is critical to the febrile response of mice to intraperitoneally administered lipopolysaccharide (LPS). We previously identified C3 and C5 as two components potentially involved in this response. This study was designed to examine whether the complement system is also pivotal in the response of mice to intravenously ... More
The effect of Lipoxin A4 on the interaction between macrophage and osteoblast: possible role in the treatment of aseptic loosening.
AuthorsLi G, Wu P, Xu Y, Yu Y, Sun L, Zhu L, Ye D
JournalBMC Musculoskelet Disord
PubMed ID19490628
Aseptic loosening (AL) is the main problem of total joints replacement (TJR) by the implantation of permanently prosthetic components. In vitro and in vivo studies have clearly demonstrated that wear debris and its byproducts could trigger inflammation in the peri-implant tissue. Lipoxins (LXs) are endogenous eicosanoids synthesized locally from arachidonate ... More
Early cellular signaling responses to axonal injury.
AuthorsLukas TJ, Wang AL, Yuan M, Neufeld AH
JournalCell Commun Signal
PubMed ID19284657
We have used optic nerve injury as a model to study early signaling events in neuronal tissue following axonal injury. Optic nerve injury results in the selective death of retinal ganglion cells (RGCs). The time course of cell death takes place over a period of days with the earliest detection ... More
The kinetics of proinflammatory cytokines in murine peritoneal macrophages infected with envelope protein-glycosylated or non-glycosylated West Nile virus.
AuthorsShirato K, Miyoshi H, Kariwa H, Takashima I
JournalVirus Res
PubMed ID16632038
The envelope (E) protein glycosylation status of the New York strain of West Nile (WN) virus is an important determinant of virus neuroinvasiveness. To elucidate the determinant of the difference between E protein-glycosylated and non-glycosylated WN virus infections, the cytokine expression of murine peritoneal macrophages infected with each virus was ... More
Left ventricular hypertrophy in mice with a cardiac-specific overexpression of interleukin-1.
AuthorsNishikawa K, Yoshida M, Kusuhara M, Ishigami N, Isoda K, Miyazaki K, Ohsuzu F
JournalAm J Physiol Heart Circ Physiol
PubMed ID16473963
Recent studies have identified the importance of proinflammatory cytokines in the development of left ventricular (LV) hypertrophy. However, the precise role of interleukin-1 (IL-1), one of the major proinflammatory cytokines, in the myocardium is not fully understood. In this study, we investigated the pathophysiological consequences of cardiac expression of IL-1 ... More
Regulation of mast cell survival by IgE.
AuthorsAsai K, Kitaura J, Kawakami Y, Yamagata N, Tsai M, Carbone DP, Liu FT, Galli SJ, Kawakami T
JournalImmunity
PubMed ID11420048
Mast cells play critical roles in hypersensitivity and in defense against certain parasites. We provide evidence that mouse mast cell survival and growth are promoted by monomeric IgE binding to its high-affinity receptor, Fc epsilon RI. Monomeric IgE does not promote DNA synthesis but suppresses the apoptosis induced by growth ... More
Epigallocatechin-3-gallate protects toluene diisocyanate-induced airway inflammation in a murine model of asthma.
AuthorsKim SH, Park HJ, Lee CM, Choi IW, Moon DO, Roh HJ, Lee HK, Park YM
JournalFEBS Lett
PubMed ID16516891
Epigallocatechin-3-gallate (EGCG), a major form of tea catechin, has anti-allergic properties. To elucidate the anti-allergic mechanisms of EGCG, we investigated its regulation of matrix metalloproteinase (MMP-9) expression in toluene diisocyanate (TDI)-inhalation lung tissues as well as TNF-alpha and Th2 cytokine (IL-5) production in BAL fluid. Compared with untreated asthmatic mice ... More
Toxicity and immunomodulatory activity of fractions of Hibiscus sabdariffa Linn (family Malvaceae) in animal models.
AuthorsFakeye T
JournalAfr J Tradit Complement Altern Med
PubMed ID20161963
This study evaluated immunomodulatory properties and the sub-acute toxicity profile of two fractions of the aqueous alcoholic extract of the dried calyx of Hibiscus sabdariffa in experimental animals. Immunomodulatory activity was evaluated using red blood cell-induced immunostimulation. The fractions were not found to be toxic after 7-day administration, though there ... More
Calcineurin activates NF-kappaB in skeletal muscle C2C12 cells.
AuthorsAlzuherri H, Chang KC
JournalCell Signal
PubMed ID12639710
Calcineurin (CnA) is an important signalling molecule in skeletal muscle, in the promotion of differentiation, slow-fibre phenotype and possibly fibre hypertrophy. We found that stable expression of constitutively active CnA in muscle C2C12 cells strongly activated NF-kappaB, a key mediator of muscle wasting. NF-kappaB activation by CnA was associated with ... More
TNF/iNOS-producing dendritic cells mediate innate immune defense against bacterial infection.
AuthorsSerbina NV, Salazar-Mather TP, Biron CA, Kuziel WA, Pamer EG
JournalImmunity
PubMed ID12871639
Dendritic cells (DCs) present microbial antigens to T cells and provide inflammatory signals that modulate T cell differentiation. While the role of DCs in adaptive immunity is well established, their involvement in innate immune defenses is less well defined. We have identified a TNF/iNOS-producing (Tip)-DC subset in spleens of Listeria ... More
Prevention of pathogenic Escherichia coli infection in mice and stimulation of macrophage activation in rats by an oral administration of probiotic Lactobacillus casei I-5.
AuthorsIshida-Fujii K, Sato R, Goto S, Yang XP, Kuboki H, Hirano S, Sato M
JournalBiosci Biotechnol Biochem
PubMed ID17420603
Lactobacillus casei I-5 isolated from an alcohol fermentation broth enhanced immunity and prevented pathogenic infection as a probiotic. Mice fed with I-5 cells for 11 days prior to an intraperitoneal challenge with pathogenic Escherichia coli Juhl exhibited a high survival rate compared with the control group. Rats fed with I-5 ... More
Immunization by Arg-gingipain A DNA vaccine protects mice against an invasive Porphyromonas gingivalis infection through regulation of interferon-gamma production.
AuthorsYonezawa H, Kato T, Kuramitsu HK, Okuda K, Ishihara K
JournalOral Microbiol Immunol
PubMed ID16101960
We previously demonstrated that a Porphyromonas gingivalis rgpA DNA vaccine induced protective immune responses against P. gingivalis infection in mice. In the present study, reduction in lethality against infection by lethal doses of P. gingivalis was observed in the rgpA DNA vaccine-immunized mice. Cytokine levels in the mouse model with ... More
Superantigenic activity is responsible for induction of coronary arteritis in mice: an animal model of Kawasaki disease.
Kawasaki disease (KD) is a multisystem vasculitis and the leading cause of acquired heart disease in children of the developed world. Lactobacillus casei cell wall extract-induced coronary arteritis in mice mirrors KD in children. Here, we report that responses to L. casei cell wall extract possess all the hallmarks of ... More
Selective stimulation of G-CSF gene expression in macrophages by a stimulatory monoclonal antibody as detected by a luciferase reporter gene assay.
AuthorsAoki Y, Sha S, Mukai H, Nishi Y
JournalJ Leukoc Biol
PubMed ID11073117
We have identified a stimulatory monoclonal antibody (mAb) from autoimmune mice that selectively stimulates granulocyte colony-stimulating factor (G-CSF) gene expression in a mouse macrophage cell line. The induction was observed not only in the cell line, but also in normal peritoneal macrophages. This mAb bound to the monocyte/macrophage cell lines ... More
Evidence that IgE molecules mediate a spectrum of effects on mast cell survival and activation via aggregation of the FcepsilonRI.
AuthorsKitaura J, Song J, Tsai M, Asai K, Maeda-Yamamoto M, Mocsai A, Kawakami Y, Liu FT, Lowell CA, Barisas BG, Galli SJ, Kawakami T
JournalProc Natl Acad Sci U S A
PubMed ID14569021
We demonstrate that binding of different IgE molecules (IgEs) to their receptor, FcepsilonRI, induces a spectrum of activation events in the absence of a specific antigen and provide evidence that such activation reflects aggregation of FcepsilonRI. Highly cytokinergic IgEs can efficiently induce production of cytokines and render mast cells resistant ... More
Apoptosis of activated CD4+ and CD8+ T cells is enhanced by co-culture with hepatocytes expressing hepatitis C virus (HCV) structural proteins through FasL induction.
A central unresolved issue in hepatitis C virus (HCV) infection is how the virus establishes chronic infection. Recent studies suggest that the liver microenvironment leads to apoptosis of activated T cells, which may be involved in the tolerance to liver allograft. Here, We report that murine hepatocytes expressing a transgene ... More
Effects of luteolin and other flavonoids on IgE-mediated allergic reactions.
AuthorsKimata M, Inagaki N, Nagai H
JournalPlanta Med
PubMed ID10705729
The anti-allergic action of luteolin was investigated in the rodent experimental allergic models. In the present study, the effects of luteolin were compared to those of baicalein, quercetin, and prednisolone. Luteolin as well as baicalein, quercetin, and prednisolone inhibited the IgE antibody-mediated biphasic cutaneous reaction (immediate phase reaction and late ... More
Effect of honey bee venom on microglial cells nitric oxide and tumor necrosis factor-alpha production stimulated by LPS.
AuthorsHan S, Lee K, Yeo J, Kweon H, Woo S, Lee M, Baek H, Kim S, Park K
JournalJ Ethnopharmacol
PubMed ID17166679
Abnormal activation of microglial cells has been implicated in various neurodegenerative diseases. Results showed that venom (KBV) produced and purified in Korea regulated lipopolysaccharides (LPS)-induced nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) in the murine microglia, BV-2 cell line. The production of proinflammatory cytokines, NO, and TNF-alpha was examined ... More
Modulation of murine macrophage function by methamphetamine.
AuthorsIn SW, Son EW, Rhee DK, Pyo S
JournalJ Toxicol Environ Health A
PubMed ID15513893
The abuse of methamphetamine (MA) is an increasingly growing problem globally and produces serious side effects. In the present study, the immunomodulating effects of MA were examined on murine peritoneal macrophages after MA (5 mg/kg body weight) was administered daily orally for 2 wk. When purified macrophages were stimulated with ... More
Glutamine preserves skeletal muscle force during an inflammatory insult.
AuthorsMeador BM, Huey KA
JournalMuscle Nerve
PubMed ID19705479
The purpose of this study was to test the hypothesis that acute glutamine (GLN) supplementation can counteract skeletal muscle contractile dysfunction occurring in response to inflammation by elevating muscle heat shock protein (Hsp) expression and reducing inflammatory cytokines. Mice received 5 mg/kg lipopolysaccharide (LPS) concurrently with 1 g/kg GLN or ... More
Anti-inflammatory activity of the methanol extract of moutan cortex in LPS-activated Raw264.7 cells.
AuthorsChun SC, Jee SY, Lee SG, Park SJ, Lee JR, Kim SC
JournalEvid Based Complement Alternat Med
PubMed ID17965763
Moutan Cortex (MCE) has been used in traditional medicine to remove heat from the blood, promote blood circulation and alleviate blood stasis. This study was conducted to evaluate the effects of MCE on regulatory mechanisms of cytokines and nitric oxide (NO) involved in immunological activity of Raw264.7 cells. Cells were ... More
Identification of gp96 as a novel target for treatment of autoimmune disease in mice.
AuthorsHan JM, Kwon NH, Lee JY, Jeong SJ, Jung HJ, Kim HR, Li Z, Kim S
JournalPLoS One
PubMed ID20352117
Heat shock proteins have been implicated as endogenous activators for dendritic cells (DCs). Chronic expression of heat shock protein gp96 on cell surfaces induces significant DC activations and systemic lupus erythematosus (SLE)-like phenotypes in mice. However, its potential as a therapeutic target against SLE remains to be evaluated. In this ... More
Prevention of lethal acute graft-versus-host disease in mice by oral administration of T helper 1 inhibitor, TAK-603.
AuthorsLu Y, Sakamaki S, Kuroda H, Kusakabe T, Konuma Y, Akiyama T, Fujimi A, Takemoto N, Nishiie K, Matsunaga T, Hirayama Y, Kato J, Kon S, Kogawa K, Niitsu Y
JournalBlood
PubMed ID11159546
Acute graft-versus-host diseases (GVHD) is a major cause of morbidity and mortality in patients undergoing allogeneic bone marrow transplantation (BMT). T helper 1 (Th1)-type cytokines such as interferon-gamma or tumor necrosis factor-alpha have been implicated in the pathogenesis of acute GVHD. TAK-603 is a new quinoline derivative, which is now ... More
Induction of cytochrome P450 2E1 [corrected] promotes liver injury in ob/ob mice.
AuthorsDey A, Cederbaum AI
JournalHepatology
PubMed ID17538970
Cytochrome P450 2E1 (CYP2E1) activates several hepatotoxins and contributes to alcoholic liver damage. Obesity is a growing health problem in the United States. The aim of the present study was to evaluate whether acetone- or pyrazole-mediated induction of CYP2E1 can potentiate liver injury in obesity. CYP2E1 protein and activity were ... More
The novel beta-defensin DEFB123 prevents lipopolysaccharide-mediated effects in vitro and in vivo.
AuthorsMotzkus D, Schulz-Maronde S, Heitland A, Schulz A, Forssmann WG, Jübner M, Maronde E
JournalFASEB J
PubMed ID16790530
Defensins are a family of secreted antimicrobial peptides proposed to directly interfere with bacterial membranes. Here we show a functional analysis of the novel beta-defensin DEFB123. A peptide comprising the beta-defensin core region was synthesized and used for our analysis. Like other beta-defensins, DEFB123 exerted antimicrobial activity against a broad ... More
Genetic dissection of the cellular pathways and signaling mechanisms in modeled tumor necrosis factor-induced Crohn's-like inflammatory bowel disease.
AuthorsKontoyiannis D, Boulougouris G, Manoloukos M, Armaka M, Apostolaki M, Pizarro T, Kotlyarov A, Forster I, Flavell R, Gaestel M, Tsichlis P, Cominelli F, Kollias G
JournalJ Exp Med
PubMed ID12486099
Recent clinical evidence demonstrated the importance of tumor necrosis factor (TNF) in the development of Crohn's disease. A mouse model for this pathology has previously been established by engineering defects in the translational control of TNF mRNA (Tnf(Delta)(ARE) mouse). Here, we show that development of intestinal pathology in this model ... More
Immune alterations in mice exposed to the herbicide simazine.
AuthorsKim KR, Son EW, Hee-Um S, Kim BO, Rhee DK, Pyo S
JournalJ Toxicol Environ Health A
PubMed ID12791541
Simazine, a triazine herbicide, was investigated for its in vivo immunomodulatory properties. Male C57Bl/6 mice were treated with vehicle or 300 or 600 mg/kg body weight (bw) simazine daily orally for 4 wk. The immune system was evaluated by the antibody response to sheep red blood cells (SRBC; plaque assay ... More
Negative feedback regulation of lipopolysaccharide-induced inducible nitric oxide synthase gene expression by heme oxygenase-1 induction in macrophages.
AuthorsAshino T, Yamanaka R, Yamamoto M, Shimokawa H, Sekikawa K, Iwakura Y, Shioda S, Numazawa S, Yoshida T
JournalMol Immunol
PubMed ID18022235
Heme oxygenase-1 (HO-1) is induced under infectious diseases in macrophages. We performed experiments using various gene deficient mouse-derived macrophages to determine a detailed induction mechanism of HO-1 by lipopolysaccharide (LPS) and the functional role of HO-1 induction in macrophages. LPS (1 microg/mL) maximally induced inducible nitric oxide synthase (iNOS) and ... More
Prostacyclin-IP signaling and prostaglandin E2-EP2/EP4 signaling both mediate joint inflammation in mouse collagen-induced arthritis.
AuthorsHonda T, Segi-Nishida E, Miyachi Y, Narumiya S
JournalJ Exp Med
PubMed ID16446378
Prostaglandin (PG)I2 (prostacyclin [PGI]) and PGE2 are abundantly present in the synovial fluid of rheumatoid arthritis (RA) patients. Although the role of PGE2 in RA has been well studied, how much PGI2 contributes to RA is little known. To examine this issue, we backcrossed mice lacking the PGI receptor (IP) ... More
Loss-of-function mutation in Toll-like receptor 4 prevents diet-induced obesity and insulin resistance.
Obesity is associated with insulin resistance and a state of abnormal inflammatory response. The Toll-like receptor (TLR)4 has an important role in inflammation and immunity, and its expression has been reported in most tissues of the body, including the insulin-sensitive ones. Because it is activated by lipopolysaccharide and saturated fatty ... More
Is IL12 necessary in experimental hypersensitivity pneumonitis?
AuthorsSchuyler M, Gott K, Cherne A
JournalInt J Exp Pathol
PubMed ID12084045
Inhalation of Saccharopolyspora rectivirgula (SR) can cause the disease Farmer's Lung, a classic example of hypersensitivity pneumonitis. Th1, but not Th2, cell lines can adoptively transfer experimental hypersensitivity pneumonitis (EHP). Substantial amounts of IL12 appear in bronchoalveolar lavage fluid (BALF) after a single intratracheal (IT) injection of SR, and SR-induced ... More
Immunomodulatory mechanism of the aqueous extract of sword brake fern (Pteris ensiformis Burm.).
AuthorsWu MJ, Weng CY, Wang L, Lian TW
JournalJ Ethnopharmacol
PubMed ID15763366
Sword brake fern (Pteris ensiformis Burm.) is an ingredient in most of the traditional herbal beverage formulas in Taiwan; however, no information is available to explain its bioactivity. The aim of this study is to elucidate the molecular pharmacological activity in the aqueous extract of sword brake fern (SBF). We ... More
Inflammation-dependent cerebral deposition of serum amyloid a protein in a mouse model of amyloidosis.
AuthorsGuo JT, Yu J, Grass D, de Beer FC, Kindy MS
JournalJ Neurosci
PubMed ID12122052
The major pathological hallmark of amyloid diseases is the presence of extracellular amyloid deposits. Serum amyloid A (SAA) is an apolipoprotein primarily produced in the liver. Serum protein levels can increase one thousandfold after inflammation. SAA is the precursor to the amyloid A protein found in deposits of systemic amyloid ... More
Cloning and characterization of a high mobility group box 1 (HMGB1) homologue protein from Schistosoma mansoni.
AuthorsGnanasekar M, Velusamy R, He YX, Ramaswamy K
JournalMol Biochem Parasitol
PubMed ID16246438
Mammalian homologue of high mobility group box chromatin protein (HMGB) 1 was identified and cloned from human parasites, Schistosoma mansoni and S. haematobium. Sequence analyses showed that the parasite HMGB1s has 35-40% identity to human and rodent HMGB1s, and 33% identity to Caenorhabditis elegans HMGB1. Parasite HMGB1s also contains an ... More
Pluronic F127 enhances the effect as an adjuvant of chitosan microspheres in the intranasal delivery of Bordetella bronchiseptica antigens containing dermonecrotoxin.
AuthorsKang ML, Jiang HL, Kang SG, Guo DD, Lee DY, Cho CS, Yoo HS
JournalVaccine
PubMed ID17485148
We have studied a vaccine delivery system in vitro and in vivo based on chitosan microspheres (CMs) prepared in the presence of selected immunomodulators, Pluronic block copolymer F127 (F127). The Bordetella bronchiseptica multiple antigens containing dermonecrotoxin (BBD), a virulent factor leading to atrophic rhinitis (AR) in swine was loaded in ... More
Antitumor activity of the TLR-5 ligand flagellin in mouse models of cancer.
AuthorsSfondrini L, Rossini A, Besusso D, Merlo A, Tagliabue E, Mènard S, Balsari A
JournalJ Immunol
PubMed ID16709820
Flagellin, the structural protein subunit of the bacterial flagellum, is specifically recognized by TLR-5 and has potent immunomodulatory effects. The antitumor effects of purified Salmonella typhimurium flagellin were evaluated in mice transplanted s.c. with a weakly immunogenic murine tumor or with its variant stably transfected to express the highly antigenic ... More
Regulation and function of CCSP during pulmonary Pseudomonas aeruginosa infection in vivo.
AuthorsHayashida S, Harrod KS, Whitsett JA
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID10956619
Clara cell secretory protein (CCSP) is a 16-kDa homodimeric polypeptide secreted by respiratory epithelial cells in the conducting airways of the lung. To assess the role of CCSP in bacterial inflammation and to discern whether CCSP expression is influenced by bacterial infection, CCSP-deficient [(-/-)] gene-targeted mice and wild-type mice were ... More
Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice.
AuthorsShin SJ, Shin SW, Kang ML, Lee DY, Yang MS, Jang YS, Yoo HS
JournalJ Vet Sci
PubMed ID17993753
We previously induced protective immune response by oral immunization with yeast expressing the ApxIIA antigen. The ApxI antigen is also an important factor in the protection against Actinobacillus pleuropneumoniae serotype 5 infection; therefore, the protective immunity in mice following oral immunization with Saccharomyces cerevisiae expressing either ApxIA (group C) or ... More
C-type natriuretic peptide attenuates bleomycin-induced pulmonary fibrosis in mice.
AuthorsMurakami S, Nagaya N, Itoh T, Fujii T, Iwase T, Hamada K, Kimura H, Kangawa K
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID15285999
C-type natriuretic peptide (CNP) has been shown to play an important role in the regulation of vascular tone and remodeling. However, the physiological role of CNP in the lung remains unknown. Accordingly, we investigated whether CNP infusion attenuates bleomycin (BLM)-induced pulmonary fibrosis in mice. After intratracheal injection of BLM or ... More
Enhancement of repopulation and hematopoiesis of bone marrow cells in irradiated mice by oral administration of PG101, a water-soluble extract from Lentinus lepideus.
AuthorsJin M, Jeon H, Jung HJ, Kim B, Shin SS, Choi JJ, Lee JK, Kang CY, Kim S
JournalExp Biol Med (Maywood)
PubMed ID12773709
PG101 is a water-soluble extract from Lentinus lepideus. It is a potential biological response modifier that activates selective cytokines in vitro, mainly by controlling cellular transcription factor NF-kappaB. Effects of PG101 were tested on bone marrow cells in irradiated mice. Mice were irradiated with a dose of 6 Gy and ... More
beta-Amyloid infusion results in delayed and age-dependent learning deficits without role of inflammation or beta-amyloid deposits.
AuthorsMalm T, Ort M, Tähtivaara L, Jukarainen N, Goldsteins G, Puoliväli J, Nurmi A, Pussinen R, Ahtoniemi T, Miettinen TK, Kanninen K, Leskinen S, Vartiainen N, Yrjänheikki J, Laatikainen R, Harris-White ME, Koistinaho M, Frautschy SA, Bures J, Koistinaho J
JournalProc Natl Acad Sci U S A
PubMed ID16723396
beta-Amyloid (Abeta) polypeptide plays a critical role in the pathogenesis of Alzheimer's disease (AD), which is characterized by progressive decline of cognitive functions, formation of Abeta deposits and neurofibrillary tangles, and loss of neurons. Increased genetic production or direct intracerebral administration of Abeta in animal models results in Abeta deposition, ... More
Neuropeptide neurotensin stimulates intestinal wound healing following chronic intestinal inflammation.
AuthorsBrun P, Mastrotto C, Beggiao E, Stefani A, Barzon L, Sturniolo GC, Palù G, Castagliuolo I
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID15764810
Because neurotensin (NT) and its high-affinity receptor (NTR1) modulate immune responses, chloride secretion, and epithelial cell proliferation, we sought to investigate their role in the repair process that follows the development of mucosal injuries during a persistent inflammation. Colonic NT and NTR1, mRNA, and protein significantly increased only after dextran ... More
Role of the FcepsilonRI beta-chain ITAM as a signal regulator for mast cell activation with monomeric IgE.
AuthorsNunomura S, Gon Y, Yoshimaru T, Suzuki Y, Nishimoto H, Kawakami T, Ra C
JournalInt Immunol
PubMed ID15944196
The beta-chain of the high-affinity receptor for IgE (FcepsilonRI) plays a crucial role for amplification of the intracellular signaling in mast cells upon FcepsilonRI cross-linking by IgE*antigen complexes (IgE*Ag). Some monomeric IgE as well as IgE*Ag stimulate FcepsilonRI-signaling pathways, leading to cell activation, whereas the biological functions of the beta-chain ... More
Keratinocyte growth factor gene transduction ameliorates acute lung injury and mortality in mice.
AuthorsBaba Y, Yazawa T, Kanegae Y, Sakamoto S, Saito I, Morimura N, Goto T, Yamada Y, Kurahashi K
JournalHum Gene Ther
PubMed ID17328680
At present there is no known effective pharmacological therapy for acute lung injury (ALI). Because keratinocyte growth factor (KGF) promotes epithelial cell growth, intratracheal administration of KGF has the possibility of restoring lung tissue integrity in injured lungs and improving patient outcomes. However, treatment using recombinant KGF protein is limited ... More
Innate immune response to adenoviral vectors is mediated by both Toll-like receptor-dependent and -independent pathways.
AuthorsZhu J, Huang X, Yang Y
JournalJ Virol
PubMed ID17229689
Recombinant adenoviral vectors have been widely used for gene therapy applications and as vaccine vehicles for treating infectious diseases such as human immunodeficiency virus disease. The innate immune response to adenoviruses represents the most significant hurdle in clinical application of adenoviral vectors for gene therapy, but it is an attractive ... More
Acute ethanol pretreatment increases FAS-mediated liver injury in mice: role of oxidative stress and CYP2E1-dependent and -independent pathways.
AuthorsWang X, Cederbaum AI
JournalFree Radic Biol Med
PubMed ID17349925
This study evaluated whether acute ethanol pretreatment potentiates Fas-mediated liver injury and if oxidative stress and CYP2E1 play a role in any enhanced hepatotoxicity. There were 3-fold increases of transaminases and more extensive apoptotic necrosis of hepatocytes and focal hemorrhages of the hepatic lobule in mice treated with Jo2 Fas ... More
S-adenosyl-L-methionine attenuates hepatotoxicity induced by agonistic Jo2 Fas antibody following CYP2E1 induction in mice.
AuthorsWang X, Cederbaum AI
JournalJ Pharmacol Exp Ther
PubMed ID16373529
S-Adenosyl-l-methionine (SAM) has been shown to be hepatoprotective against many toxic agents. Its possible effectiveness in protecting against CYP2E1-dependent toxicity is not known. We recently reported that treatment of mice with pyrazole to induce CYP2E1 increased hepatotoxicity produced by Fas agonistic Jo2 antibody. The current study was designed to investigate ... More
Cytochrome P4502E1 sensitizes to tumor necrosis factor alpha-induced liver injury through activation of mitogen-activated protein kinases in mice.
AuthorsWu D, Cederbaum A
JournalHepatology
PubMed ID18095305
The goal of this study was to evaluate the role of mitogen-activated protein kinase (MAPK) in cytochrome P4502E1 (CYP2E1) potentiation of lipopolysaccharide or tumor necrosis factor alpha (TNF-alpha)-induced liver injury. Treatment of C57/BL/6 mice with pyrazole (PY) plus lipopolysaccharide (LPS) induced liver injury compared with mice treated with PY or ... More