DNase I, RNase-free (supplied with MnCl2) (1 U/μL) - FAQs

Will DNase I (Cat. No. EN0525) cleave DNA in the presence of either Mg2+ or Mn2+?

Yes,in the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion. In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides.