RNase A/T1 Mix - FAQs

What is your recommended RNase digestion mixture with Thermo Scientific RNase A/T1 Mix for RNase protection assay?

We recommend 10 mM Tris-HCl (pH 7.5), 300 mM NaCl, 5 mM EDTA (pH 7.5), 20 µL of RNase A/T1 Mix per 1 mL of reaction mixture.