RNase I (10 U/μL), 5,000 units - FAQs

What are your recommendations for removal of RNA from DNA solutions using Thermo Scientific RNase I?

1. Add 15-20 u of RNase I per 1 µg of RNA. RNase I is ≥ 90% active within pH range 7.0-8.8 at salt concentration 100-200 mM. Incubation with RNase I can be performed simultaneously with the digestion of DNA by restriction endonucleases.

2. Incubate at 37 degrees C for 30 minutes.

3. Purify DNA by spin column or phenol/chloroform extraction.