What are the recommended reaction time and temperature for inactivating RNAse A with Thermo Scientific RiboLock RNase Inhibitor?
The activity of RiboLock RNAse Inhibitor in principle is temperature-independent because there are no chemical reactions involved. At both 25 degrees C and 37 degrees C, the same quantity of RiboLock RNAse Inhibitor is needed to quench a given amount of RNase A.