DNA Polymerase I (10 U/μL), 2,500 units - FAQs

What are the differences between Thermo Scientific DNA Polymerase I, Klenow Fragment, and Klenow Fragment (exo-)?

Thermo Scientific DNA Polymerase I possesses 5′?3′ DNA synthesis activity, 3′?5′ exonuclease (proofreading) activity, and 5′?3′ exonuclease activity. Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5′?3′ polymerase activity and 3′?5′ exonuclease (proofreading) activity, but lacks 5′?3′ exonuclease activity. Klenow Fragment, exo-, is also the large fragment of DNA polymerase I. It exhibits 5′?3′ polymerase activity, but lacks the 3′?5′ and 5′?3′ exonuclease activities.