T7 RNA 聚合酶，HC (200 U/μL)
T7 RNA 聚合酶，HC (200 U/μL)
Thermo Scientific™

T7 RNA 聚合酶，HC (200 U/μL)

Thermo Scientific Bacteriophage T7 RNA polymerase is a DNA-dependent RNA polymerase with strict specificity for its respective double-stranded promoters. It了解更多信息
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货号数量
EP011325,000 U
货号 EP0113
价格（CNY)
2,712.00
飞享价
Ends: 31-Dec-2025
3,874.00
共减 1,162.00 (30%)
Each
添加至购物车
数量:
25,000 U
请求批量或定制报价
价格（CNY)
2,712.00
飞享价
Ends: 31-Dec-2025
3,874.00
共减 1,162.00 (30%)
Each
添加至购物车
Thermo Scientific Bacteriophage T7 RNA polymerase is a DNA-dependent RNA polymerase with strict specificity for its respective double-stranded promoters. It catalyzes the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.

Highlights

• Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Applications

Synthesis of unlabeled and labeled RNA that can be used:

• For hybridization, in vitro RNA translation
• As aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing
• In studies of RNA secondary structure and RNA-protein interactions, RNA splicing

Consensus promoter sequence:
T7: TAATACGACTCACTATAGGGAGA
仅供科研使用。不可用于诊断程序。
规格
最大浓度>200 U/µL
数量25,000 U
聚合酶T7 RNA 聚合酶
Unit SizeEach

常见问题解答 (FAQ)

What is the advantage of using TranscriptAid T7 High Yield Transcription Kit over Thermo Scientific T7 RNA Polymerase with a standard transcription buffer?

TranscriptAid T7 High Yield Transcription Kit is designed to produce both long and short transcripts for applications that require large yields of RNA (milligram amounts). The kit can achieve 10 times greater amount than from conventional in vitro transcription reactions with T7 RNA polymerase due to its unique proprietary enzyme and buffer formulations. Please note that TranscriptAid T7 High Yield Transcription Kit is not recommended for generation of radioactively labelled RNA. Due to large quantities of RNA synthesized with the kit, generation of high specific activity radiolabeled probes would require prohibitively large amounts of radiolabeled nucleotide.