DreamTaq™ Hot Start DNA Polymerase, 500 U - FAQs

查看更多产品信息 DreamTaq™ Hot Start DNA Polymerase - FAQs (EP1703, EP1711, EP1701, EP1712, EP1702, EP1713)

6 个常见问题解答

Which nucleotide analogues can be used with DreamTaq Hot Start DNA Polymerase?

DreamTaq Hot Start DNA Polymerase can incorporate dUTP and a variety of modified nucleotides, such as: dITP, 7-deaza-dGTP, fluorescein-12-dUTP, Biotin-11-dUTP, dm5CTP, alpha-thio-dCTP, and aminoallyl-dUTP.

Are DreamTaq buffers the same for DreamTaq DNA Polymerase and DreamTaq Hot Start DNA Polymerase?

Yes. 10X DreamTaq Buffer and 10X DreamTaq Green Buffer are the same for both polymerases.

My experiments require extra DreamTaq Buffer. Do you offer the buffer as a separate product?

Yes, DreamTaq Buffer (Cat. No. B65, 4 x 1.25 mL) and DreamTaq Green Buffer (Cat. No. B71, 4 x 1.25 mL) are available as stand-alone products.

Can DreamTaq Hot Start DNA Polymerase be used for amplification of bisulfite converted DNA?

Yes. DreamTaq Hot Start DNA Polymerase can read uracil in the template strand and therefore can be used for amplification of bisulfite converted DNA.

What is the limit of amplicon size using DreamTaq Hot Start DNA Polymerase?

DreamTaq Hot Start DNA Polymerase amplifies up to 6 kb from human genomic DNA and up to 20 kb from lambda DNA with high yields and specificity. Amplification of even longer fragments up to 9 kb from human genomic DNA has been demonstrated, but may require additional optimization of reaction conditions and primer design.

Does DreamTaq Hot Start DNA Polymerase add the non-template dependent 3'-A overhang?

Yes. DreamTaq Hot Start DNA Polymerase generates 3'-A overhangs.