Potentiation of porcine circovirus 2-induced postweaning multisystemic wasting syndrome by porcine parvovirus is associated with excessive production of tumor necrosis factor-alpha.
AuthorsKim J, Ha Y, Chae C
JournalVet Pathol
PubMed ID16966450
'This study investigated the potentiation of porcine circovirus 2 (PCV2)-induced postweaning multisystemic wasting syndrome by porcine parvovirus (PPV) and found it was associated with excessive production of tumor necrosis factor-alpha (TNF-alpha). Colostrum-deprived conventional pigs were inoculated intranasally with PCV2 or PPV alone or in combination (PCV2 and PPV). In vitro ... More
In vitro and in vivo effects of an immunomodulator composed of Escherichia coli lipopolysaccharide and Propionibacterium granulosum-inactivated cells in pigs.
AuthorsPappaterra Mendoza GJ, Mateu de Antonio E, Novell Badal ME, Martín Castillo M, Casal Fàbrega J, Marca Puig J
JournalJ Vet Med B Infect Dis Vet Public Health
PubMed ID11075550
'The in vitro cytokine profiles of porcine alveolar macrophages and peripheral blood mononuclear cells (PBMC) were examined by reverse transcription-polymerase chain reaction or enzyme-linked immunosorbent assay after stimulation with the immunomodulatory compound INMD [lipopolysaccharide (LPS) and Propionibacterium granulosum]. Expression of interleukin-1 (IL-1), IL-6, IL-12 and tumour necrosis factor-alpha (TNF-alpha), but ... More
The effects of thermal environment and spray-dried plasma on the acute-phase response of pigs challenged with lipopolysaccharide.
AuthorsFrank JW, Carroll JA, Allee GL, Zannelli ME
JournalJ Anim Sci
PubMed ID12772843
'Forty barrows (TR4 x C22) were weaned at 17 d of age (BW = 6.27 +/- 0.30 kg), housed (two pigs/pen) in a thermal-neutral environment (TN; constant 26.7 degrees C), and fed diets with or without 7% (as-fed basis) spray-dried plasma (SDP). On d 7, one pig/ pen was moved ... More
Pathogenicity of Cryptosporidium parvum--evaluation of an animal infection model.
'With the intention of developing a standardised method for assessment of pathogenicity of Cryptosporidium parvum, the CPB-0 isolate was studied by propagation in 1-day-old calves followed by inoculation into specific pathogen free (SPF) piglets. The experiment was repeated. Diarrhoea and shedding of oocysts were seen in all animals infected with ... More
Impact of different modalities of continuous venovenous hemofiltration on sepsis-induced alterations in experimental pancreatitis.
AuthorsYekebas EF, Strate T, Zolmajd S, Eisenberger CF, Erbersdobler A, Saalmüller A, Steffani K, Busch C, Elsner HA, Engelhardt M, Gillesen A, Meins J, The M, Knoefel WT, Izbicki JR
JournalKidney Int
PubMed ID12371983
'Continuous venovenous hemofiltration (CVVH) is assumed to attenuate systemic complications in septic diseases. The impact of different treatment intensities of CVVH on immunologic and systemic alterations in experimental pancreatitis was evaluated.' ... More
Changes in macrophages in spleen and lymph nodes during acute African swine fever: expression of cytokines.
'To gain further insight into the pathogenesis of African swine fever (ASF), the cytokine expression by macrophages in spleen and lymph nodes were examined. Twenty-one piglets were inoculated with the highly virulent isolate Spain-70 of ASF virus and killed in groups at 1-7 post-inoculation days (pid). An increase in the ... More
Interleukin-10 gene therapy-mediated amelioration of bacterial pneumonia.
AuthorsMorrison DF, Foss DL, Murtaugh MP
JournalInfect Immun
PubMed ID10899882
'Respiratory infection by Actinobacillus pleuropneumoniae causes a highly pathogenic necrotizing pleuropneumonia with severe edema, hemorrhage and fever. Acute infection is characterized by expression of inflammatory cytokines, including interleukin-1 (IL-1), IL-6 and IL-8. To determine if high level production of inflammatory cytokines contributed to disease pathogenesis, we investigated if inhibiting macrophage ... More
The evolution of ischemic spinal cord injury in function, cytoarchitecture, and inflammation and the effects of adenosine A2A receptor activation.
AuthorsReece TB, Okonkwo DO, Ellman PI, Warren PS, Smith RL, Hawkins AS, Linden J, Kron IL, Tribble CG, Kern JA
JournalJ Thorac Cardiovasc Surg
PubMed ID15573078
Spinal cord ischemia/reperfusion injury involves multiple factors that may be modulated by adenosine A 2A receptor activation. This study defines injury progression in terms of function, cytoarchitecture, and inflammation and assesses whether adenosine A 2A receptor activation by ATL-146e limits injury progression. ... More
Attenuation of sepsis-related immunoparalysis by continuous veno-venous hemofiltration in experimental porcine pancreatitis.
AuthorsYekebas EF, Eisenberger CF, Ohnesorge H, Saalmüller A, Elsner HA, Engelhardt M, Gillesen A, Meins J, The M, Strate T, Busch C, Knoefel WT, Bloechle C, Izbicki JR
JournalCrit Care Med
PubMed ID11445702
In light of evidence suggesting that hemofiltration favorably influences septic diseases by removing sepsis mediators, the impact of different modalities of continuous veno-venous hemofiltration (CVVH) on outcome and immunologic derangements in porcine pancreatogenic sepsis was evaluated. ... More
Cardiopulmonary bypass elicits a pro- and anti-inflammatory cytokine response and impaired neutrophil chemotaxis in neonatal pigs.
AuthorsBrix-Christensen V, Petersen TK, Ravn HB, Hjortdal VE, Andersen NT, Tønnesen E
JournalActa Anaesthesiol Scand
PubMed ID11300377
Cardiopulmonary bypass (CPB) induces a systemic inflammatory response and organ dysfunction, especially in children. Plasma concentration of inflammatory markers are increased in response to the trauma of cardiac surgery and CPB. The aim of the present study was to investigate whether the CPB procedure in itself elicits increased levels of ... More
Systematic reaction to electrolytic treatment of pig livers in vivo.
AuthorsBerry D, Garcea G, Chong C, Silman E, Finch G, Dennison A, Maddern GJ
JournalANZ J Surg
PubMed ID15230798
Only a minority of secondary liver tumours are amenable to segmental resection and as a result, considerable research has been focused on developing ablative methods to destroy liver metastases. Many of these methods are limited by the development of a systemic inflammatory response mediated by cytokines such as interleukin-8 (IL-8) ... More
Induction of experimental allergic encephalomyelitis in the NIH minipig.
AuthorsSinger BA, Tresser NJ, Frank JA, McFarland HF, Biddison WE
JournalJ Neuroimmunol
PubMed ID10713359
Experimental allergic encephalomyelitis (EAE) was induced in the NIH minipig to create a large animal model of multiple sclerosis with a well-characterized immune system. Sixteen NIH minipigs were inoculated with minipig spinal cord homogenate (SCH). The clinical course was primarily monophasic, but re-induction was possible. CNS and blood lymphocytes specifically ... More
Chlortetracycline modulates acute phase response of ex vivo perfused pig livers, and inhibits TNF-alpha secretion by isolated Kupffer cells.
AuthorsAkunda JK, Johnson E, Ahrens FA, Kramer TT
JournalComp Immunol Microbiol Infect Dis
PubMed ID11247048
Tetracyclines have been shown to have anti-inflammatory effects in addition to their antimicrobial action. We investigated the effects of in vivo administration of chlortetracycline (CTC) on ex vivo perfused pig livers. The retention and clearance of Salmonella choleraesuis, production of acute phase proteins C-reactive protein (CRP), and haptoglobin (HPG) by ... More
Direct cell-to-cell contact between Kupffer cells and hepatocytes augments endotoxin-induced hepatic injury.
AuthorsHoebe KH, Witkamp RF, Fink-Gremmels J, Van Miert AS, Monshouwer M
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID11254499
This study focuses on the importance of direct contact between Kupffer cells (KCs) and hepatocytes (HCs) during the hepatic inflammatory response using an in vitro approach. The lipopolysaccharide (LPS)-induced inflammatory response in monocultures of porcine HCs and KCs were compared with cocultures prepared either with direct contact between KCs and ... More
Adiponectin differentially regulates cytokines in porcine macrophages.
AuthorsWulster-Radcliffe MC, Ajuwon KM, Wang J, Christian JA, Spurlock ME
JournalBiochem Biophys Res Commun
PubMed ID15033490
Adiponectin, an adipocyte-derived hormone, attenuates the production of TNFalpha by activated human macrophages. In the present study, we used porcine blood-derived macrophages to test the hypothesis that the anti-inflammatory action of adiponectin includes suppression of IL6 and an induction of IL10. Adiponectin suppressed both TNFalpha and IL6 production in macrophages ... More
Reduction of porcine reproductive and respiratory syndrome virus (PRRSV) infection in swine alveolar macrophages by porcine circovirus 2 (PCV2)-induced interferon-alpha.
AuthorsChang HW, Jeng CR, Liu JJ, Lin TL, Chang CC, Chia MY, Tsai YC, Pang VF
JournalVet Microbiol
PubMed ID15936905
Two common viral pathogens of swine, namely, porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV), were investigated in regard to their effects on monolayer cultures of swine alveolar macrophages (AMs). The purpose was to identify selected cellular changes and responses potentially associated with the clinical ... More