HSP60 Human ProcartaPlex™ Simplex Kit - FAQs

Unfortunately, we do not currently offer a Mac-compatible version of the ProcartaPlex Analyst Software. ProcartaPlex Analyst Software is compatible with PC, or xPONENT Software is available on Luminex instruments to analyze your data.

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Sorry, the FlowCytomix product line has been discontinued. However, we offer a new antibody and magnetic bead-based detection system, i.e., ProcartaPlex assays for multiplex protein quantitation using the Luminex instrument platform.

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With everything working smoothly, here is the approximate read time for one plate on different Luminex instruments:

- FLEXMAP 3D Systems and INTELLIFLEX Systems will read 96 well plates in ~20 min, and 384 well plates in ~75 min.
- Luminex 100/200 Systems will read 96 well plates in ~40 min.
- MAGPIX System will read 96 well plates in ~50-60 min. Please note that these are estimates for the read times of the plates only, and do not include time for calibration or set-up of the instrument.

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In general, sensitivity between Simplex and Multiplex ProcartaPlex assay kits is comparable.

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We supply the standard protein in a lyophilized form. Lyophilization is a dehydration process that preserves the perishable standard. We have demonstrated that often, the amount of starting protein prior to lyophilization and that of the final product after lyophilization varies (this could be different from analyte to analyte). To ensure that we deliver the most quantitative and reproducible product, we always calibrate the standard after lyophilization and therefore, the concentration range can vary from lot to lot of the standard.

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No. Storage of the beads at temperatures below 0 degrees C will damage the beads and render them unusable.

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We validate and release our ProcartaPlex assays with a minimum bead count of 50. In our experience, the assays also work with a bead count of 20 though we have not validated them at this bead count.

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The sensitivity information is based on the 2 hr incubation with samples.

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To minimize inter-assay coefficient of variation between two independent assay plates, it is very important that the same operator performs the assays. Also, we recommend that you follow the incubation times exactly as they are stated in the manual in order to get consistent results. Other tips for minimizing inter-assay coefficient of variation include performing a standard curve in duplicate on each plate and using the same kit lot.

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In-house, we test ProcartaPlex assays with RPMI + 0.5% FCS. You would have to empirically determine if your specific medium will interfere with the assay.

Note: Our customers have successfully used media containing up to 5% FBS with ProcartaPlex assays. As bovine serum contains TGF beta, the use of FBS or FCS should be avoided with TGF beta ProcartaPlex assays.

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Open the Bio-Plex Manager and click on “File”, then “Document Export Properties”, then “Output CSV Format” and set “Analytes Labels” to “Name (Region)”. Choose the desired file “Destination”, and click “OK”. Click “Document Export”, then “Export”, and import the generated .CSV file into ProcartaPlex Analysis App on Thermo Fisher Connect: https://apps.thermofisher.com/apps/procartaplex

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We have not specifically tested ProcartaPlex assays with tear samples in-house. However, here is a reference from the literature describing the use of ProcartaPlex assays with tear samples (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161422/pdf/pone.0107370.pdf).

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We have tested breast milk samples in-house using the serum ProcartaPlex protocol and found no general interference with the breast milk matrix. Please note that we have only tested a few analytes (TNFa, IL-10, TNFR), however, there is no obvious reason why other analytes should not work as well.

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The size of the beads in our ProcartaPlex assays is 6.5 µm.

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