免疫肿瘤学检查点 7-Plex 小鼠 ProcartaPlex™ 检测组合 2
免疫肿瘤学检查点 7-Plex 小鼠 ProcartaPlex™ 检测组合 2
Invitrogen™

免疫肿瘤学检查点 7-Plex 小鼠 ProcartaPlex™ 检测组合 2

小鼠免疫肿瘤学检查点 7-Plex ProcartaPlex 检测组合 2 可通过使用 Luminex xMAP 技术分析在单个孔中分析 7了解更多信息
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货号数量
EPX070-20835-90196 次测试
货号 EPX070-20835-901
价格(CNY)
-
数量:
96 次测试

小鼠免疫肿瘤学检查点 7-Plex ProcartaPlex 检测组合 2 可通过使用 Luminex xMAP 技术分析在单个孔中分析 7 种蛋白靶标进行癌症研究。同时检测这些免疫共刺激和共抑制检查点靶标可提供关于癌症和自身免疫过程中的免疫激活或稳态信息。该检测组合以即用型规格提供,配有以 1X 浓度配制的一小瓶捕获和检测试剂,所需的移液和实验设置更少。它不能与 Simplex 或其他检测组合结合使用。它补充了免疫肿瘤学检查点 4-plex ProcartaPlex 检测组合(货号 EPX040-20830-901),从而可在同一研究领域提供 4 个额外蛋白靶标。

对 ProcartaPlex 预配置检测组合进行了广泛的分析物结合、干扰和交叉反应性检测,以提供较高水平的验证和精密度。所有 ProcartaPlex 检测组合均配有进行检测所需的试剂。

靶标列表 [微珠区域]:CD137L (4-1BBL) [74]、CD152 (CTLA4) [77]、CD276 (B7-H3) [67]、CD80 [15]、PD-1 [73]、PD-L1 [29]、PD-L2 [53]

关于用于 Luminex 平台的 ProcartaPlex 测定
ProcartaPlex 免疫测定基于夹心式 ELISA 的原理,使用与一种蛋白的不同抗原决定簇结合的两种高度特异性抗体,通过 Luminex 仪器同时定量测定所有蛋白靶标。ProcartaPlex 多通路检测只需 25 μL 血浆或血清,或 50 μL 细胞培养物上清液,只需四小时即可获得分析结果。

其特性包括:
• 可重现的可靠结果—经验证检测组合符合较高行业标准,包括蛋白靶标可结合性和交叉反应性检测
• 每个样品可得到更多结果—在单个 25–50 μL 样品中同时测量多种蛋白靶标
• 成熟的 Luminex 技术 — 经常被引用的蛋白检测和定量多通路平台

ProcartaPlex 检测利用 Luminex xMAP(多分析物分析)技术在单个 25–50 μL 样品(血浆、血清、细胞培养上清液和其他体液)中同时检测和定量多达 80 种蛋白靶标。

ProcartaPlex 检测中的 Luminex 微珠用精确比例的红色和红外荧光基团进行内部染色,生成光谱独特的标记,可通过 Luminex xMAP 检测系统(如 Luminex 200、FLEXMAP 3D 和 MAGPIX)识别。与夹心ELISA类似,ProcartaPlex测定使用匹配的抗体对来鉴定目的蛋白质。在多通路测定中,每种光谱独特的微珠用单一靶蛋白特异性抗体标记,并且用生物素化抗体和链霉素亲和素–R-藻红蛋白 (RPE) 识别结合的蛋白。蛋白特异性抗体与独特微珠偶联可用于在单孔中分析多个靶标。

ProcartaPlex 检测与 ELISA 之间最显著的区别在于 ProcartaPlex 检测中的捕获抗体与微珠偶联并且不吸附于微孔板孔中,因此 ProcartaPlex 检测试剂在溶液中自由漂浮。例如,对于检测,Luminex 200 仪器包含两种激光,一种用于区分每个微珠的光谱特征,另一种用于定量 RPE 荧光的量,其与

仅供科研使用。不可用于诊断程序。
规格
测定范围参见检验报告
检测灵敏度参见检验报告
适用于(设备)Luminex™ 仪器
产品规格多通路试剂盒
产品线ProcartaPlex™
样品类型血清、血浆、细胞培养物上清液, Plasma, Cell Culture Supernatants
样品体积血清,血浆:25 μL;CCS:50 μL
运输条件湿冰
可组合性不可结合使用
产品类型多通路检测组合
数量96 次测试
研究领域Immunology, Oncology
种属小鼠
Unit SizeEach
内容与储存
  • 2 小瓶小鼠标准品混合物 F(冻干)
  • 1 小瓶捕获微珠混合物 (1 X)
  • 1 小瓶生物素化检测抗体混合物 (1 X)
  • 1 瓶读取缓冲液 (1X)
  • 1 瓶洗涤缓冲液 (10 x)
  • 1 瓶链霉素亲和素-PE (1 X)
  • 1 瓶 Universal Assay Buffer (1 X)
  • 8 联排管
  • 黏性封板膜
  • 平底 96 孔板,黑色
  • 储存于 2°C 至 8°C

常见问题解答 (FAQ)

Do you offer a Mac-compatible version of the ProcartaPlex Analyst Software?

Unfortunately, we do not currently offer a Mac-compatible version of the ProcartaPlex Analyst Software. ProcartaPlex Analyst Software is compatible with PC, or xPONENT Software is available on Luminex instruments to analyze your data.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do you offer FlowCytomix assays?

Sorry, the FlowCytomix product line has been discontinued. However, we offer a new antibody and magnetic bead-based detection system, i.e., ProcartaPlex assays for multiplex protein quantitation using the Luminex instrument platform.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How long does it take to acquire ProcartaPlex assay data on the different Luminex instruments?

With everything working smoothly, here is the approximate read time for one plate on different Luminex instruments:

- FLEXMAP 3D Systems and INTELLIFLEX Systems will read 96 well plates in ~20 min, and 384 well plates in ~75 min.
- Luminex 100/200 Systems will read 96 well plates in ~40 min.
- MAGPIX System will read 96 well plates in ~50-60 min. Please note that these are estimates for the read times of the plates only, and do not include time for calibration or set-up of the instrument.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is the sensitivity value for a Multiplex ProcartaPlex assay different when compared to the sensitivity for those same analytes assayed as Simplex assays?

In general, sensitivity between Simplex and Multiplex ProcartaPlex assay kits is comparable.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why does the ProcartaPlex ULOQ (Upper Limit of Quantitation) and LLOQ (Lower Limit of Quantitation) change between different lots of standards?

We supply the standard protein in a lyophilized form. Lyophilization is a dehydration process that preserves the perishable standard. We have demonstrated that often, the amount of starting protein prior to lyophilization and that of the final product after lyophilization varies (this could be different from analyte to analyte). To ensure that we deliver the most quantitative and reproducible product, we always calibrate the standard after lyophilization and therefore, the concentration range can vary from lot to lot of the standard.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.