Anti-inflammatory activity of macrolide antibiotics.
AuthorsIanaro A, Ialenti A, Maffia P, Sautebin L, Rombolà L, Carnuccio R, Iuvone T, D'Acquisto F, Di Rosa M
JournalJ Pharmacol Exp Ther
PubMed ID10604943
'The effect of four macrolide antibiotics (roxithromycin, clarithromycin, erythromycin, and azithromycin) on the generation of some mediators and cytokines involved in the inflammatory process has been studied both in vivo and in vitro. Rat carrageenin pleurisy was used as a model of acute inflammation, and the macrolides were administered (10, ... More
Transient maintenance in bioreactor improves health of neuronal cells.
AuthorsDi Loreto S, Sebastiani P, Benedetti E, Zimmitti V, Caracciolo V, Amicarelli F, Cimini A, Adorno D
JournalIn Vitro Cell Dev Biol Anim
PubMed ID16848632
'To examine whether a neuronal cell suspension can be held in vitro for a relatively short period without compromising survival rates and functionality, we have set up an experimental protocol planning 24 h of suspension culture in a rotary wall vessel (RWV) bioreactor before plating in a conventional adherent system. ... More
Differential responses of visceral and subcutaneous fat depots to nutrients.
AuthorsEinstein FH, Atzmon G, Yang XM, Ma XH, Rincon M, Rudin E, Muzumdar R, Barzilai N
JournalDiabetes
PubMed ID15734842
'Increased visceral adiposity is a pivotal component of the metabolic syndrome. Differential gene expression patterns of fat-derived peptides (FDPs) in visceral fat and subcutaneous fat have been characterized in the fasting state. Here we examined whether delivery of nutrients differentially affects the expression of FDPs in visceral fat versus subcutaneous ... More
Effects of transient loss of shear stress on blood-brain barrier endothelium: role of nitric oxide and IL-6.
AuthorsKrizanac-Bengez L, Kapural M, Parkinson F, Cucullo L, Hossain M, Mayberg MR, Janigro D
JournalBrain Res
PubMed ID12834884
'Loss of blood-brain barrier (BBB) function may contribute to post-ischemic cerebral injury by yet unknown mechanisms. Ischemia is associated with anoxia, aglycemia and loss of flow (i.e. shearing forces). We tested the hypothesis that loss of shear stress alone does not acutely affect BBB function due to a protective cascade ... More
Regulation of bone metabolism in immunosuppressant (FK506)-treated rats.
AuthorsKirino S, Fukunaga J, Ikegami S, Tsuboi H, Kimata M, Nakata N, Nakano M, Ueno T, Mizukawa N, Sugahara T
JournalJ Bone Miner Metab
PubMed ID15490265
'After organ transplantation, severe osteoporosis is occasionally seen, and the use of immunosuppressants is thought to be one of the causes of such osteoporosis. In the present study, we investigated the effects of FK506 monotherapy on bones and determined the mechanism of onset of osteoporosis, both by assessing chronological changes ... More
Otomicroscopic findings and systemic interleukin-6 levels in relation to etiologic agent during experimental acute otitis media.
AuthorsFoglé-Ansson M, White P, Hermansson A, Melhus A
JournalAPMIS
PubMed ID16689828
'The aim of the present study was to explore whether it was possible to differentiate the clinical course and the otomicroscopic appearance of acute otitis media (AOM) caused by common otitis pathogens in an animal model. Systemic interleukin (IL)-6 levels as early markers for bacterial AOM were also studied. Four ... More
Mechanisms of endotoxin-induced NO, IL-6, and TNF-alpha production in activated rat hepatic stellate cells: role of p38 MAPK.
AuthorsThirunavukkarasu C, Watkins SC, Gandhi CR
JournalHepatology
PubMed ID16871588
'Compelling experimental evidence indicates that the interactions between endotoxin and hepatic stellate cells (HSCs) can play a significant role in the pathogenesis of liver disease. Endotoxin-induced release of a multifunctional mediator NO (via inducible NO synthase) and the proinflammatory cytokines tumor necrosis factor alpha (TNF-alpha) and interleukin (IL)-6 by HSCs ... More
Pretreatment with olprinone hydrochloride, a phosphodiesterase III inhibitor, attenuates lipopolysaccharide-induced lung injury via an anti-inflammatory effect.
AuthorsKoike T, Nadeen Qutab M, Tsuchida M, Takekubo M, Saito M, Hayashi J
JournalPulm Pharmacol Ther
PubMed ID17434327
'Acute respiratory distress syndrome is characterized by neutrophil accumulation in the lungs and the activation of several cytokines produced by macrophages. Olprinone hydrochloride, a specific phosphodiesterase III inhibitor, has anti-inflammatory effects and inhibits the activation of macrophages, in addition to its inotropic and vasodilatory effects. The purpose of this study ... More
Mast cell activation by Mycobacterium tuberculosis: mediator release and role of CD48.
AuthorsMuñoz S, Hernández-Pando R, Abraham SN, Enciso JA
JournalJ Immunol
PubMed ID12759438
'Mast cells (MC) are abundant in the lung and other peripheral tissue, where they participate in inflammatory processes against bacterial infections. Like other effector cells of the innate immune system, MC interact directly with a wide variety of infectious agents. This interaction results in MC activation and inflammatory mediator release. ... More
Selective downregulation of hepatic cytochrome P450 expression and activity in a rat model of inflammatory pain.
This study was designed to examine the effect of Freund's complete adjuvant (FCA)-induced inflammation on liver P450 expression and activities in the first 7 days that followed a single FCA injection in the rat hindpaw. ... More
Effect of porto-systemic shunting on NOS expression after extended hepatectomy in rats.
AuthorsHayashi H, Shimizu K, Tani T, Takamura H, Takeshita M, Nakamura K, Ninomiya I, Fushida S, Harada S, Kayahara M
JournalHepatol Res
PubMed ID18713274
Several surgical procedures have been developed for reducing portal vein pressure to prevent postoperative liver injury. Nitric oxide synthase expression (NOS) induced by elevation of portal vein pressure is thought to play an important role in liver regeneration, but the details are not well understood. ... More
IL-10 attenuates hepatic I/R injury and promotes hepatocyte proliferation.
AuthorsDinant S, Veteläinen RL, Florquin S, van Vliet AK, van Gulik TM
JournalJ Surg Res
PubMed ID17543989
One of the most important determinants of the outcome of hepatic ischemia and reperfusion (I/R) injury is the onset of the inflammatory response. Interleukin-10 (IL-10) is a potent anti-inflammatory cytokine. It inhibits the production of interleukin-6 (IL-6), which however, also is involved in priming hepatocyte proliferation. The aim of this ... More
Effects of caloric restriction and exercise on age-related, chronic inflammation assessed by C-reactive protein and interleukin-6.
AuthorsKalani R, Judge S, Carter C, Pahor M, Leeuwenburgh C
JournalJ Gerontol A Biol Sci Med Sci
PubMed ID16567369
Chronic inflammation is associated with the aging process and numerous age-related pathologies. We evaluated the effects of age, caloric restriction (CR), and exercise on plasma C-reactive protein (CRP), interleukin-6, and total antioxidant capacity in Fisher 344 rats. The inflammatory markers were analyzed using enzyme-linked immunosorbent assays (ELISA), while total antioxidant ... More
Influence of cytokines and growth factors in ANG II-mediated collagen upregulation by fibroblasts in rats: role of myocytes.
AuthorsSarkar S, Vellaichamy E, Young D, Sen S
JournalAm J Physiol Heart Circ Physiol
PubMed ID15059775
Abnormal stiffness and altered cardiac function arising from abnormal collagen deposition occur in hypertrophy and heart failure. ANG II has been shown to play a role in this process. To evaluate the mechanism, we developed an in vitro model by subjecting fibroblasts to ANG II treatment in the presence or ... More
Extended transgene expression from a nonintegrating adenoviral vector containing retroviral elements.
We studied the effects of specific retroviral elements in a first-generation serotype 5 adenoviral (Ad5) vector, AdLTR(2)EF1alpha-hEPO. This vector contains 858 base pair (bp) [251-bp envelope sequence plus 607-bp long-terminal repeat (LTR)] from Moloney murine leukemia virus (MoMLV), upstream of the human elongation factor-1alpha (EF1alpha) promoter and human erythropoietin (hEPO) ... More
Nitric oxide (NO)-releasing naproxen (HCT-3012 [(S)-6-methoxy-alpha-methyl-2-naphthaleneacetic Acid 4-(nitrooxy)butyl ester]) interactions with aspirin in gastric mucosa of arthritic rats reveal a role for aspirin-triggered lipoxin, prostaglandins, and NO in gastric protection.
AuthorsFiorucci S, Di Lorenzo A, Renga B, Farneti S, Morelli A, Cirino G
JournalJ Pharmacol Exp Ther
PubMed ID15297470
Administration of selective and nonselective cyclooxygenase (COX)-2 inhibitors to rheumatoid arthritis patients taking low doses of acetylsalicylic acid (ASA) for cardiovascular prevention associates with increased risk of gastrointestinal bleeding. The present study was undertaken to investigate whether administration of HCT-3012 [(S)-6-methoxy-alpha-methyl-2-naphthaleneacetic acid 4-(nitrooxy)butyl ester], a nitric oxide (NO)-releasing derivative of ... More
Epithelial cells respond to proteolytic and non-proteolytic detachment by enhancing interleukin-6 responses.
AuthorsMiller TL, McGee DW
JournalImmunology
PubMed ID11849320
Intestinal inflammatory disease or infection often results in the loss of the epithelial layer as a result mainly of the action of proteases, including the leucocyte serine proteinases (neutrophil elastase), lysosomal cathepsins and the matrix metalloproteinases from recruited inflammatory cells. Previous studies have shown that bronchial or intestinal epithelial cells ... More
Loss of shear stress induces leukocyte-mediated cytokine release and blood-brain barrier failure in dynamic in vitro blood-brain barrier model.
AuthorsKrizanac-Bengez L, Mayberg MR, Cunningham E, Hossain M, Ponnampalam S, Parkinson FE, Janigro D
JournalJ Cell Physiol
PubMed ID15920760
Brain ischemia is associated with an acute release of pro-inflammatory cytokines, notably TNF-alpha and IL-6 and failure of the blood-brain barrier. Shear stress, hypoxia-hypoglycemia, and blood leukocytes play a significant role in blood-brain barrier failure during transient or permanent ischemia. However, these mechanisms have not been studied as independent variables ... More