FilmTracer™ FM™ 1-43 Green 生物膜细胞染色剂

FM dyes are lipophilic styryl compounds used in a wide variety of studies involving plasma membrane and vesiculation. The water-soluble了解更多信息

货号	数量
F10317	各 1

货号 F10317

价格（CNY)

4,996.00

Ends: 31-Dec-2025

6,772.00

共减 1,776.00 (26%)

添加至购物车

价格（CNY)

4,996.00

Ends: 31-Dec-2025

6,772.00

共减 1,776.00 (26%)

添加至购物车

FM dyes are lipophilic styryl compounds used in a wide variety of studies involving plasma membrane and vesiculation. The water-soluble FM® dyes are virtually nonfluorescent in aqueous media and are believed to insert into the surface membrane where they become intensely fluorescent. FM® 1-43 stain has been used successfully to stain the cell bodies specifically in a complex biofilm mileu, including Pseudomonas aeruginosa, Escherichia coli, Staphylococcus sp., Acidothiobacillus caldus, and Vibrio cholerae.

仅供科研使用。不可用于诊断程序。

检测方法荧光

染料类型FM 1-43⁄脂质

发射可见光

激发波长范围472⁄580

适用于（设备）共聚焦显微镜、荧光显微镜、流式细胞仪、微孔板读数仪、荧光成像仪

产品线FilmTracer™、Molecular Probes™

数量各 1

运输条件室温

标签类型其他标签或染料

产品类型染料

亚细胞定位细胞膜&脂质, Lipids

Unit SizeEach

内容与储存

室温储存；干燥；避光

常见问题解答 (FAQ)

The product sheet for FM 1-43 Dye states that the maximum for excitation is 510 nm and emissions is 626 nm, but the spectrum on the product page shows a peak at 470 nm and 580 nm. Can you clarify what the excitation and emission maxima is for this dye?

FM lipophilic styryl dyes are known to shift their spectral properties upon binding to membranes. These dyes exhibit an appreciable solvent and environmental dependency in both free and membrane-bound forms. The emissions of the membrane-bound dye shifts to a shorter wavelength compared to the emission in the solvent.

In solvent, FM 1-43 Dye (Cat. Nos. T35356, T3163, F35355, F10317) absorbs in the range of fluorescein (~490 nm) and initially emits at around 626-636 nm. However, upon membrane integration, it exhibits emission in the 565-590 nm range. Because this dye exhibits a large Stokes shift, it does not perfectly fit into a green or orange fluorophore profile, but is rather a yellow-fluorescent dye, and may require a long-pass emission filter.

Note: FM 1-43 Dye is often used with typical fluorescein or GFP optical filter sets for biological imaging but is poorly excited through tetramethylrhodamine filters.

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