Dynamics of the action of biocides in Pseudomonas aeruginosa biofilms.
AuthorsBridier A, Dubois-Brissonnet F, Greub G, Thomas V, Briandet R,
JournalAntimicrob Agents Chemother
PubMed ID21422224
'The biocidal activity of peracetic acid (PAA) and benzalkonium chloride (BAC) on Pseudomonas aeruginosa biofilms was investigated by using a recently developed confocal laser scanning microscopy (CLSM) method that enables the direct and real-time visualization of cell inactivation within the structure. This technique is based on monitoring the loss of ... More
Novel amelogenin-releasing hydrogel for remineralization of enamel artificial caries.
AuthorsFan Y, Wen ZT, Liao S, Lallier T, Hagan JL, Twomley JT, Zhang JF, Sun Z, Xu X,
JournalJ Bioact Compat Polym
PubMed ID23338820
Recently, the use of recombinant full-length amelogenin protein in combination with fluoride has shown promising results in the formation of densely packed enamel-like structures. In this study, amelogenin (rP172)-releasing hydrogels containing calcium, phosphate, and fluoride were investigated for remineralization efficacy using in vitro early enamel caries models. The hydrogels were ... More
Antimicrobial penetration and efficacy in an in vitro oral biofilm model.
AuthorsCorbin A, Pitts B, Parker A, Stewart PS,
JournalAntimicrob Agents Chemother
PubMed ID21537022
The penetration and overall efficacy of six mouthrinse actives was evaluated by using an in vitro flow cell oral biofilm model. The technique involved preloading biofilm cells with a green fluorescent dye that leaked out as the cells were permeabilized by a treatment. The loss of green color, and of ... More
C-terminal amino acids of alpha-melanocyte-stimulating hormone are requisite for its antibacterial activity against Staphylococcus aureus.
AuthorsSingh M, Mukhopadhyay K,
JournalAntimicrob Agents Chemother
PubMed ID21282427
Alpha-melanocyte-stimulating hormone (a-MSH) is an endogenous neuropeptide that is known for its anti-inflammatory and antipyretic activities. We recently demonstrated that a-MSH possesses staphylocidal activity and causes bacterial membrane damage. To understand the role of its amino acid sequences in the staphylocidal mechanism, in the present study we investigated the antimicrobial ... More
Local antibiotic delivery with bovine cancellous chips.
Infected bone defects and osteomyelitis are encountered frequently in trauma cases. Currently, the standard of care for osteomyelitis cases is prolonged systemic antibiotic therapy and implantation of antibiotic carrier beads. However, this method requires a secondary surgery to remove the beads after the infection has cleared. In the present study ... More
Spatial and temporal patterns of biocide action against Staphylococcus epidermidis biofilms.
AuthorsDavison WM, Pitts B, Stewart PS,
JournalAntimicrob Agents Chemother
PubMed ID20457816
The dynamic antimicrobial action of chlorine, a quaternary ammonium compound, glutaraldehyde, and nisin within biofilm cell clusters of Staphylococcus epidermidis was investigated using time-lapse confocal scanning laser microscopy. The technique allowed for the simultaneous imaging of changes in biofilm structure and disruption of cellular membrane integrity through the loss of ... More
CcpA and LacD.1 affect temporal regulation of Streptococcus pyogenes virulence genes.
AuthorsKietzman CC, Caparon MG,
JournalInfect Immun
PubMed ID19841076
Production of H(2)O(2) follows a growth phase-dependent pattern that mimics that of many virulence factors of Streptococcus pyogenes. To gain greater insight into mechanisms coupling virulence factor expression to growth phase, we investigated the molecular basis for H(2)O(2) generation and its regulation. Deletion of the gene encoding lactate oxidase (lctO) ... More
Comparison of different live/dead stainings for detection and quantification of adherent microorganisms in the initial oral biofilm.
AuthorsTawakoli PN, Al-Ahmad A, Hoth-Hannig W, Hannig M, Hannig C,
JournalClin Oral Investig
PubMed ID22821430
The aim of the present study was to investigate different fluorescence-based, two-color viability assays for visualization and quantification of initial bacterial adherence and to establish reliable alternatives to the ethidium bromide staining procedure. Bacterial colonization was attained in situ on bovine enamel slabs (n?=?6 subjects). Five different live/dead assays were ... More