Fluo-4 Direct™ 钙含量检测试剂盒
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引用和文献 (13)
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Fluo-4 Direct™ 钙含量检测试剂盒

Fluo-4 Direct™ 钙含量测定试剂盒专门用于提供均相荧光钙含量测定试剂盒，其具有以下特点：1.轻松加载至细胞内 2.获得较大的测定窗口，以及3.在完全培养基中抑制钙指示剂生成的背景荧光，对特定细胞荧光几乎没有影响 这种先进的配方可以在含血清的培养基中以简单的“单纯加样”形式进行测定。Fluo-4 Direct™ 是 Fluo-4 钙含量检测试剂系列的第三款产品了解更多信息
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货号数量
F10471100 ml
F10472100 ml
F104731000 ml
货号 F10471
价格（CNY)
5,118.00
飞享价
Ends: 31-Dec-2025
6,892.00
共减 1,774.00 (26%)
Each
添加至购物车
数量:
100 ml
价格（CNY)
5,118.00
飞享价
Ends: 31-Dec-2025
6,892.00
共减 1,774.00 (26%)
Each
添加至购物车
Fluo-4 Direct™ 钙含量测定试剂盒专门用于提供均相荧光钙含量测定试剂盒，其具有以下特点：
1.轻松加载至细胞内
2.获得较大的测定窗口，以及
3.在完全培养基中抑制钙指示剂生成的背景荧光，对特定细胞荧光几乎没有影响

这种先进的配方可以在含血清的培养基中以简单的“单纯加样”形式进行测定。Fluo-4 Direct™ 是 Fluo-4 钙含量检测试剂系列的第三款产品。Fluo-4 AM 和 Fluo-4 NW 都需要在测定试剂盒检测前除去培养基，但 Fluo-4 NW 通过使用便于细胞加载的 PowerLoad™ 试剂增加了便利性。Fluo-4 Direct™ 钙含量测定试剂盒与 Fluo-4 NW 类似，其配方同样含有 PowerLoad™ 以方便细胞加载，但独特之处在于其能够在完全培养基内使用，并且可以有效抑制背景荧光，而不会影响测定中产生的特定细胞荧光。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型基于荧光染料
形式粉末
修改化学修饰
数量100 ml
运输条件室温
子细胞定位细胞核、细胞器、细胞质
颜色绿色
发射可见
适用于（应用）钙含量测定试剂盒
适用于（设备）共聚焦显微镜, 荧光显微镜, 高内涵分析仪, HTS 读数仪, 微孔板读数仪, 荧光成像仪
产品线Molecular Probes
产品类型基于细胞的钙通量
Unit SizeEach
内容与储存
  • 10 × 10 ml Fluo-4 Direct™ 钙含量测定试剂（组分 A）
  • 2 × 77 mg 丙磺舒（组份 B）
  • 200 ml Fluo-4 Direct™ 钙含量测定缓冲液（组分 C）
  • 保存于 ≤-20°C 。干燥避光储存。

常见问题解答 (FAQ)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (13)

引用和文献
Abstract
High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates.
Authors:Biggs MJ, Milone MC, Santos LC, Gondarenko A, Wind SJ,
Journal:J R Soc Interface
PubMed ID:21490003
'T-cell activation via antigen presentation is associated with the formation of a macromolecular membrane assembly termed the immunological synapse (IS). The genesis of the IS and the onset of juxtacrine signalling is characterized by the formation of cell membrane microclusters and the organization of such into segregated microdomains. A central ... More
Regulation of cytosolic phospholipase A2 (cPLA2alpha) and its association with cell proliferation in human lens epithelial cells.
Authors:Wang Y, Xing KY, Lou MF,
Journal:Invest Ophthalmol Vis Sci
PubMed ID:21896865
'To investigate the molecular mechanism for cytosolic phospholipase A2 (cPLA(2)a) regulation and its association to platelet-derived growth factor (PDGF)-induced cell proliferation. cPLA(2)a was examined using human lens epithelial (HLE) B3 cells. Reactive oxygen species (ROS) generation induced by PDGF was analyzed by luminescence assay. Cell proliferation was measured by cell ... More
20-Hydroxyeicosatetraenoic acid (20-HETE) is a novel activator of transient receptor potential vanilloid 1 (TRPV1) channel.
Authors:Wen H, Östman J, Bubb KJ, Panayiotou C, Priestley JV, Baker MD, Ahluwalia A,
Journal:J Biol Chem
PubMed ID:22389490
'TRPV1 is a member of the transient receptor potential ion channel family and is gated by capsaicin, the pungent component of chili pepper. It is expressed predominantly in small diameter peripheral nerve fibers and is activated by noxious temperatures >42 °C. 20-Hydroxyeicosatetraenoic acid (20-HETE) is a cytochrome P-450 4A/4F-derived metabolite ... More
Gq/5-HT2c receptor signals activate a local GABAergic inhibitory feedback circuit to modulate serotonergic firing and anxiety in mice.
Authors:Spoida K, Masseck OA, Deneris ES, Herlitze S,
Journal:
PubMed ID:24733892
Serotonin 2c receptors (5-HT2c-Rs) are drug targets for certain mental disorders, including schizophrenia, depression, and anxiety. 5-HT2c-Rs are expressed throughout the brain, making it difficult to link behavioral changes to circuit specific receptor expression. Various 5-HT-Rs, including 5-HT2c-Rs, are found in the dorsal raphe nucleus (DRN); however, the function of ... More
Small molecule inhibition of PAX3-FOXO1 through AKT activation suppresses malignant phenotypes of alveolar rhabdomyosarcoma.
Authors:Jothi M, Mal M, Keller C, Mal AK,
Journal:
PubMed ID:24107448
Alveolar rhabdomyosarcoma comprises a rare highly malignant tumor presumed to be associated with skeletal muscle lineage in children. The hallmark of the majority of alveolar rhabdomyosarcoma is a chromosomal translocation that generates the PAX3-FOXO1 fusion protein, which is an oncogenic transcription factor responsible for the development of the malignant phenotype ... More
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