Simultaneous measurement of intracellular pH and Ca2+ in insulin-secreting cells by spectral imaging microscopy.
Authors:Martínez-Zaguilán R,Gurulé MW,Lynch RM
Journal:The American journal of physiology
PubMed ID:8967445
Arabidopsis abi1-1 and abi2-1 phosphatase mutations reduce abscisic acid-induced cytoplasmic calcium rises in guard cells.
Authors:Allen GJ,Kuchitsu K,Chu SP,Murata Y,Schroeder JI
Journal:The Plant cell
PubMed ID:10488243
Elevations in cytoplasmic calcium ([Ca(2)+](cyt)) are an important component of early abscisic acid (ABA) signal transduction. To determine whether defined mutations in ABA signal transduction affect [Ca(2)+](cyt) signaling, the Ca(2)+-sensitive fluorescent dye fura 2 was loaded into the cytoplasm of Arabidopsis guard cells. Oscillations in [Ca(2)+](cyt) could be induced when ... More
Ca2+ transients in cardiac myocytes measured with high and low affinity Ca2+ indicators.
Authors:Berlin JR,Konishi M
Journal:Biophysical journal
PubMed ID:8274651
Intracellular calcium ion ([Ca2+]i) transients were measured in voltage-clamped rat cardiac myocytes with fura-2 or furaptra to quantitate rapid changes in [Ca2+]i. Patch electrode solutions contained the K+ salt of fura-2 (50 microM) or furaptra (300 microM). With identical experimental conditions, peak amplitude of stimulated [Ca2+]i transients in furaptra-loaded myocytes ... More
Evidence for role of cytosolic free calcium in hypoxia-induced proximal tubule injury.
Authors:Kribben A, Wieder ED, Wetzels JF, Yu L, Gengaro PE, Burke TJ, Schrier RW
Journal:J Clin Invest
PubMed ID:8182125
The role of cytosolic free Ca2+ ([Ca2+]i) in hypoxic injury was investigated in rat proximal tubules. [Ca2+]i was measured using fura-2 and cell injury was estimated with propidium iodide (PI) in individual tubules using video imaging fluorescence microscopy. [Ca2+]i increased from approximately 170 to approximately 390 nM during 5 min ... More
Hyposmotic activation hyperpolarizes outer hair cells of guinea pig cochlea.
Authors:Harada N, Ernst A, Zenner HP
Journal:Brain Res
PubMed ID:8348313
The electrophysiological responses of isolated guinea pig outer hair cells (OHCs) to hyposmotic activation were studied using the whole-cell patch-clamp technique. The cell swelling by hyposmotic activation hyperpolarized OHCs by 6.6 +/- 2.3 mV from the resting membrane potential of -58.5 +/- 5.9 mV (n = 48). This hyperpolarization was ... More