In vivo non-linear optical (NLO) imaging in live rabbit eyes using the Heidelberg Two-Photon Laser Ophthalmoscope.
AuthorsHao M, Flynn K, Nien-Shy C, Jester BE, Winkler M, Brown DJ, La Schiazza O, Bille J, Jester JV,
JournalExp Eye Res
PubMed ID20558159
Imaging of non-linear optical (NLO) signals generated from the eye using ultrafast pulsed lasers has been limited to the study of ex vivo tissues because of the use of conventional microscopes with slow scan speeds. The purpose of this study was to evaluate the ability of a novel, high scan ... More
Accelerated phagocytosis of amyloid-beta by mouse and human microglia overexpressing the macrophage colony-stimulating factor receptor.
AuthorsMitrasinovic OM, Murphy GM Jr.
JournalJ Biol Chem
PubMed ID12032144
Microglia surrounding A beta plaques in Alzheimer's disease and in the APPV717F transgenic mouse model of Alzheimer's disease have enhanced immunoreactivity for the macrophage colony-stimulating factor receptor (M-CSFR), encoded by the proto-oncogene c-fms. Increased expression of M-CSFR on cultured microglia results in proliferation and release of pro-inflammatory cytokines and expression ... More
Accurate and fiducial-marker-free correction for three-dimensional chromatic shift in biological fluorescence microscopy.
AuthorsMatsuda A, Schermelleh L, Hirano Y, Haraguchi T, Hiraoka Y
JournalSci Rep
PubMed ID29765093
'Correction of chromatic shift is necessary for precise registration of multicolor fluorescence images of biological specimens. New emerging technologies in fluorescence microscopy with increasing spatial resolution and penetration depth have prompted the need for more accurate methods to correct chromatic aberration. However, the amount of chromatic shift of the region ... More
Tissue Damage Signaling Is a Prerequisite for Protective Neutrophil Recruitment to Microbial Infection in Zebrafish.
AuthorsHuang C, Niethammer P
JournalImmunity
PubMed ID29768163
Tissue damage and infection are deemed likewise triggers of innate immune responses. But whereas neutrophil responses to microbes are generally protective, neutrophil recruitment into damaged tissues without infection is deleterious. Why neutrophils respond to tissue damage and not just to microbes is unknown. Is it a flaw of the innate ... More