Effects of neurotoxic and neuroprotective agents on peripheral nerve regeneration assayed by time-lapse imaging in vivo.
AuthorsPan YA, Misgeld T, Lichtman JW, Sanes JR
JournalJ Neurosci
PubMed ID14673013
'A direct histological assay of axonal regeneration would have many advantages over currently available behavioral, electrophysiological, and radiometric assays. We show that peripheral sensory axons marked with the yellow fluorescent protein in transgenic mice can be viewed transcutaneously in superficial nerves. Degenerating and regenerating axons can be followed in live ... More
In vivo non-linear optical (NLO) imaging in live rabbit eyes using the Heidelberg Two-Photon Laser Ophthalmoscope.
AuthorsHao M, Flynn K, Nien-Shy C, Jester BE, Winkler M, Brown DJ, La Schiazza O, Bille J, Jester JV,
JournalExp Eye Res
PubMed ID20558159
Imaging of non-linear optical (NLO) signals generated from the eye using ultrafast pulsed lasers has been limited to the study of ex vivo tissues because of the use of conventional microscopes with slow scan speeds. The purpose of this study was to evaluate the ability of a novel, high scan ... More