Assembly of a fibronectin matrix by adherent platelets stimulated by lysophosphatidic acid and other agonists.
AuthorsOlorundare OE, Peyruchaud O, Albrecht RM, Mosher DF
JournalBlood
PubMed ID11418470
'Lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are agonists of the endothelial differentiation gene (Edg) family of G-protein-coupled receptors. LPA and S1P are generated by platelet activation during blood coagulation. Both lipids induce assembly of exogenous fibronectin (FN) by fibroblasts. This study examined whether LPA and S1P stimulate binding and assembly ... More
Fibrinogen deposition at the postischemic vessel wall promotes platelet adhesion during ischemia-reperfusion in vivo.
'Following ischemia-reperfusion (I/R), platelet adhesion is thought to represent the initial event leading to remodeling and reocclusion of the vasculature. The mechanisms underlying platelet adhesion to the endothelium have not been completely established. Endothelial cells rendered ischemic acquire a procoagulant phenotype, characterized by fibrinogen accumulation. Therefore, we evaluated whether fibrinogen ... More
Ultrathin self-assembled fibrin sheets.
AuthorsO'Brien ET, Falvo MR, Millard D, Eastwood B, Taylor RM, Superfine R,
JournalProc Natl Acad Sci U S A
PubMed ID19052234
'Fibrin polymerizes into the fibrous network that is the major structural component of blood clots and thrombi. We demonstrate that fibrin from three different species can also spontaneously polymerize into extensive, molecularly thin, 2D sheets. Sheet assembly occurs in physiologic buffers on both hydrophobic and hydrophilic surfaces, but is routinely ... More
Reprogramming cell shape with laser nano-patterning.
AuthorsVignaud T, Galland R, Tseng Q, Blanchoin L, Colombelli J, Théry M,
JournalJ Cell Sci
PubMed ID22357956
Cell shape in vitro can be directed by geometrically defined micropatterned adhesion substrates. However conventional methods are limited by the fixed micropattern design, which cannot recapitulate the dynamic changes of the cell microenvironment. Here, we manipulate the shape of living cells in real time by using a tightly focused pulsed ... More
Kindlin-3 is essential for integrin activation and platelet aggregation.
AuthorsMoser M, Nieswandt B, Ussar S, Pozgajova M, Fässler R,
JournalNat Med
PubMed ID18278053
Integrin-mediated platelet adhesion and aggregation are essential for sealing injured blood vessels and preventing blood loss, and excessive platelet aggregation can initiate arterial thrombosis, causing heart attacks and stroke. To ensure that platelets aggregate only at injury sites, integrins on circulating platelets exist in a low-affinity state and shift to ... More
Pluronic additives: a solution to sticky problems in digital microfluidics.
AuthorsLuk VN, Mo GCh, Wheeler AR,
JournalLangmuir
PubMed ID18481875
Digital microfluidics (DMF) is a promising technique for carrying out miniaturized, automated biochemical assays in which discrete droplets of reagents are actuated on the surface of an array of electrodes. A limitation for DMF is nonspecific protein adsorption to device surfaces, which interferes with assay fidelity and can cause droplets ... More
Dynamic, electronically switchable surfaces for membrane protein microarrays.
AuthorsTang CS, Dusseiller M, Makohliso S, Heuschkel M, Sharma S, Keller B, Vörös J
JournalAnal Chem
PubMed ID16448043
Microarray technology is a powerful tool that provides a high throughput of bioanalytical information within a single experiment. These miniaturized and parallelized binding assays are highly sensitive and have found widespread popularity especially during the genomic era. However, as drug diagnostics studies are often targeted at membrane proteins, the current ... More
An αIIb β3 antagonist prevents thrombosis without causing Fc receptor γ-chain IIa-mediated thrombocytopenia.
Authors
JournalJ Thromb Haemost
PubMed ID28815933
SARS-CoV-2 spike protein S1 induces fibrin(ogen) resistant to fibrinolysis: implications for microclot formation in COVID-19.
Authors
JournalBiosci Rep
PubMed ID34328172
Parkinson's Disease: A Systemic Inflammatory Disease Accompanied by Bacterial Inflammagens.
Authors
JournalFront Aging Neurosci
PubMed ID31507404
Lubricant-infused directly engraved nano-microstructures for mechanically durable endoscope lens with anti-biofouling and anti-fogging properties.
Authors
JournalSci Rep
PubMed ID33060752
Human Placental Mesenchymal Stem/Stromal cells (pMSCs) inhibit agonist-induced platelet functions reducing atherosclerosis and thrombosis phenotypes.
Authors
JournalJ Cell Mol Med
PubMed ID34535958
Gingipain R1 and Lipopolysaccharide From Porphyromonas gingivalis Have Major Effects on Blood Clot Morphology and Mechanics.
Authors
JournalFront Immunol
PubMed ID32793214
Enhanced β2-microglobulin binding of a navigator" molecule bearing a single-chain variable fragment antibody for artificial switching of metabolic processing pathways."
Authors
JournalBiomater Sci
PubMed ID34231557
Effects of Diabetes Mellitus on Fibrin Clot Structure and Mechanics in a Model of Acute Neutrophil Extracellular Traps (NETs) Formation.
Authors
JournalInt J Mol Sci
PubMed ID32993159
Engineering transferrable microvascular meshes for subcutaneous islet transplantation.
Authors
JournalNat Commun
PubMed ID31601796
A microengineered vascularized bleeding model that integrates the principal components of hemostasis.