Search
Search
View additional product information for Flow Cytometry Sub-micron Particle Size Reference Kit - FAQs (F13839)
17 product FAQs found
试剂盒中的绿色染料是细胞通透性核酸染色剂，会标记所有细胞。红色染料不能渗透进细胞，只能对膜受损的细胞（死细胞）进行染色。因此，任何具有红色信号的细胞会被认为是死亡的。您的细胞可能会出现有些只有红色信号，有些有红色和绿色信号，有些只有绿色信号的现象。有时红色染料会置换绿色染料。在任何情况下，红色细胞都是死亡的。
另外，绿色染料可能漏进红色通道。进行单色染色，同时在绿色和红色滤光片下检查确定漏光程度。为了避免这种漏光的情况，请使用低浓度的染料；如果可能的话，使用窄带通滤光片。
这里有几种办法。我们有两种荧光试剂盒可用于细菌计数：Live/Dead BacLight Bacterial Viability and Counting Kit（货号L34856）；Bacteria Counting kit, for flow cytometry（货号B7277）。另一个选择是Flow Cytometry Sub-micron Particle Size Reference Kit（货号F13839）。
Attune NxT声波聚焦流式细胞仪检测的下限是0.5 µm。
Attune NxT自动进样器是Attune NxT声波聚焦流式细胞仪的可选附件，能够快速分析至多384份样品。对于不同形式平板——无论96孔还是384孔板——它都具有广泛的兼容性。内置智能探针，最大限度避免堵塞和截留（<0.5%），防止损坏仪器。该仪器通过通气而不是振荡混合，确保样品的均质性，同时维持细胞的活性。它还可作为Attune NxT流式细胞仪关机程序的一个步骤来执行自动清洗。无论采用何种取样方法（试管或平板）和数据采集速率，它都能提供一致的数据。
•模块化设计—多种配置可选，可现场升级。
•节省时间—在不降低数据质量的前提下，速度提高10倍以上。
•简化样品准备流程—无需洗涤，无裂解选项，流体不堵塞。
•可实现独特的应用—可针对广泛的细胞类型和样本构建复杂的实验方案。
针对多参数分析应用，Attune NxT声波聚焦流式细胞仪可以选择最多4个激光器和14色配置，采用模块化系统设计来满足大部分试验需求和实验室预算。创新的光路设计，有助于确保四个空间分隔的激光器精确对齐样品流，确保长期的数据一致性、优越的性能和出色的可靠性。仪器可配备多达4个具有平顶光束的固态激光器（405 nm、488 nm、561 nm和637 nm）。
Attune NxT声波聚焦流式细胞仪的声波聚焦技术采用超声波辐射压力（>2 MHz），将微粒运送至样品流中心。之后，这股预聚焦流注入到为样品提供额外流体动力学压力的鞘液流中。这种“声波辅助流体动力学聚焦”技术可以在任何进样速率的情况下都保证样品流中心范围集中、激光照度均一。在传统的依赖于流体动力学聚焦技术的流式细胞仪中，样品中心需要变宽来适应流速的增加，从而导致激光照度不均一。
细胞计数是一种测量细胞和微粒物理化学特性的方法。流式细胞术可在流式细胞仪内使细胞或微粒单独通过激光来测定其特性，它可用于测定单细胞，针对样品中的每个细胞提供独立的测定结果。此外，还可提供这些样品特性的统计分布结果。 流式细胞仪由三个子系统组成：流体系统、光学系统和电子系统。流体系统负责使细胞移动，从而对其进行分析。光学系统负责产生和收集光信号。电子系统负责将合适的光学信号转化为电子信号，以便进行计算机分析。
We recommend mixing the beads thoroughly before use and using a slower flow rate.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Yes. Charged groups are on the surface making the beads water-dispersible.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The dye is dispersed throughout the polystyrene matrix.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The green dye in the kit will label all the cells as it is a cell-permeant nucleic acid stain. The red dye is not cell permeant, and will only stain the cells with compromised membranes (dead cells). Therefore, any cells with red signal will be considered dead. It is possible that you will have some cells that are only red, some that are red and green, and some that are only green. Sometimes the red dye will displace the green dye. In any case, any red cells are dead.
Also, the green dye emission may bleed through into the red channel. Do a single-color staining and examine under both green and red filter sets to determine the level of bleedthrough. To avoid this bleedthrough, use a lower concentration of dye, and, if possible, use narrow bandpass filters.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
There are several options. We have two fluorescence based kits that are useful for bacterial counting: Live/Dead BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856) and Bacteria Counting kit, for flow cytometry (Cat. No. B7277). Another option is the Flow Cytometry Sub-micron Particle Size Reference Kit (Cat. No. F13839).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The smallest size that you can detect with the Attune NxT Acoustic Focusing Cytometer is 0.5 µm.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The Attune NxT Autosampler, an optional accessory for the Attune NxT Acoustic Focusing Cytometer, enables rapid processing of up to 384 samples. It has broad compatibility with different plate formats, both 96- and 384-well plates. It has an intelligent probe designed to minimize clogging and carryover (<0.5%) and to prevent damage to the instrument. It mixes by aspiration rather than shaking to ensure homogeneity of the sample and maintain cell viability. Is performs automated cleaning as part of the shutdown process of the Attune NxT Cytometer. It provides consistent data regardless of sampling method (tube vs. plate) and collection rate.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
-Modular design - Multiple configurations available - field upgradable.
-Save time - 10X faster speeds with no loss in data quality.
-Simplified sample prep - No wash, no lyse options, non-clogging fluidics.
-Enables unique applications - Complex protocols on a broad range of cell types and samples.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
With the option to be configured with up to 4 lasers and 14 colors for multi-parameter analysis the Attune NxT Acoustic Focusing Cytometer was designed as a modular system to fit most experimental needs and lab budgets. The novel design of the optical path helps ensure precise fixed alignment of four spatially separated lasers onto the sample stream enabling consistency in data over time, superior performance, and superior reliability. The instrument can be configured with up to 4 solid-state lasers (405 nm, 488 nm, 561 nm, and 637 nm) with flat top beam profiles.
The Attune NxT Flow Cytometer's acoustic focusing uses ultrasonic radiation pressure (>
2 MHz) to transport particles into the center of the sample stream. This pre-focused stream is then injected into the sheath stream, which supplies an additional hydrodynamic pressure to the sample. The combination of these two forces- termed acoustic-assisted hydrodynamic focusing-results in a narrow core stream and uniform laser illumination, regardless of the sample input rate. In traditional cytometers that rely solely on hydrodynamic focusing, the sample core widens to accommodate the increases in flow rate, which results in less uniform laser light illumination.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Cytometry is the measurement of physical or chemical characteristics of cells or particles. Flow cytometry measures these characteristics of cells or particles as they individually pass lasers in a flow cytometer instrument. Flow cytometry is performed on single cells, providing discrete measurements for each cell in the sample. It also provides a statistical distribution of the measured characteristics of the sample.
A flow cytometer is made up of three subsystems: fluidics, optics, and electronics. Fluidics moves the cells and introduces them for interrogation. Optics generates and collects the light signals. Electronics converts the optical signals to proportional electronic signals for computer analysis.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.