Fura-2，AM，FluoroPure™ 级 - 特殊包装

引用和文献 (4399)

Invitrogen™

Fura-2，AM，FluoroPure™ 级 - 特殊包装

因为它在我们位于俄勒冈州尤金的 ISO 9001—认证工厂进行生产，我们可以保证通过 HPLC 法测得的 FluoroPure 级 Fura-2 AM 纯度大于或等于了解更多信息

货号	数量
F14185	20 x 50 μg

货号 F14185

价格（CNY)

5,348.00

Ends: 31-Dec-2025

7,014.00

共减 1,666.00 (24%)

添加至购物车

20 x 50 μg

价格（CNY)

5,348.00

Ends: 31-Dec-2025

7,014.00

共减 1,666.00 (24%)

添加至购物车

因为它在我们位于俄勒冈州尤金的 ISO 9001—认证工厂进行生产，我们可以保证通过 HPLC 法测得的 FluoroPure 级 Fura-2 AM 纯度大于或等于 98%。

了解更多有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的信息›

仅供科研使用。不可用于诊断程序。

检测方法荧光

染料类型基于荧光染料

数量20 x 50 μg

运输条件室温

适用于（应用）荧光探针

适用于（设备）荧光显微镜

产品线FluoroPure

产品类型Fura-2 AM

Unit SizeEach

内容与储存

在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The K_d value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (4399)

引用和文献

Abstract

Mechanisms of photoswitch conjugation and light activation of an ionotropic glutamate receptor.

Authors:Gorostiza P,Volgraf M,Numano R,Szobota S,Trauner D,Isacoff EY

Journal:Proceedings of the National Academy of Sciences of the United States of America

PubMed ID:17578923

The analysis of cell signaling requires the rapid and selective manipulation of protein function. We have synthesized photoswitches that covalently modify target proteins and reversibly present and withdraw a ligand from its binding site due to photoisomerization of an azobenzene linker. We describe here the properties of a glutamate photoswitch ... More

Simultaneous measurement of intracellular pH and Ca2+ in insulin-secreting cells by spectral imaging microscopy.

Authors:Martínez-Zaguilán R,Gurulé MW,Lynch RM

Journal:The American journal of physiology

PubMed ID:8967445

Functional implications of calcium permeability of the channel formed by pannexin 1.

Authors:Vanden Abeele F,Bidaux G,Gordienko D,Beck B,Panchin YV,Baranova AV,Ivanov DV,Skryma R,Prevarskaya N

Journal:The Journal of cell biology

PubMed ID:16908669

Although human pannexins (PanX) are homologous to gap junction molecules, their physiological function in vertebrates remains poorly understood. Our results demonstrate that overexpression of PanX1 results in the formation of Ca(2+)-permeable gap junction channels between adjacent cells, thus, allowing direct intercellular Ca(2+) diffusion and facilitating intercellular Ca(2+) wave propagation. More ... More

Rescue of vasopressin V2 receptor mutants by chemical chaperones: specificity and mechanism.

Authors:Robben JH,Sze M,Knoers NV,Deen PM

Journal:Molecular biology of the cell

PubMed ID:16267275

Because missense mutations in genetic diseases of membrane proteins often result in endoplasmic reticulum (ER) retention of functional proteins, drug-induced rescue of their cell surface expression and understanding the underlying mechanism are of clinical value. To study this, we tested chemical chaperones and sarco(endo)plasmic reticulum Ca(2+) ATPase pump inhibitors on ... More

Disruption of a single copy of the SERCA2 gene results in altered Ca2+ homeostasis and cardiomyocyte function.

Authors:Ji Y,Lalli MJ,Babu GJ,Xu Y,Kirkpatrick DL,Liu LH,Chiamvimonvat N,Walsh RA,Shull GE,Periasamy M

Journal:The Journal of biological chemistry

PubMed ID:10970890

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