Fluo-4 AM - 高通量筛选包
Fluo-4 AM - 高通量筛选包
Invitrogen™

Fluo-4 AM - 高通量筛选包

标记钙指示剂是结合 Ca2+ 离子后荧光强度增加的分子。Fluo-3 应用在流式细胞分析上,如涉及笼状螯合剂光活化、第二信使和神经递质的实验以及细胞水平的药理学筛选,用于 Ca2+ 的空间动力学成像。Fluo-4了解更多信息
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货号数量
F142025 x 1 mg
货号 F14202
价格(CNY)
18,558.00
飞享价
Ends: 31-Dec-2025
24,990.00
共减 6,432.00 (26%)
Each
添加至购物车
数量:
5 x 1 mg
价格(CNY)
18,558.00
飞享价
Ends: 31-Dec-2025
24,990.00
共减 6,432.00 (26%)
Each
添加至购物车
标记钙指示剂是结合 Ca2+ 离子后荧光强度增加的分子。Fluo-3 应用在流式细胞分析上,如涉及笼状螯合剂光活化、第二信使和神经递质的实验以及细胞水平的药理学筛选,用于 Ca2+ 的空间动力学成像。Fluo-4 是 fluo-3 的类似物,其中两个氯取代基被氟取代,导致在 488 nm 波长处的荧光激发增强,因此荧光信号水平更高。将溶解后的指示剂直接加入含有培养细胞的培养皿中,即可向细胞上样 AM 酯形式的这类钙离子指示剂。这些指示剂适用于荧光和共聚焦显微镜、流式细胞分析和微孔板筛选应用。

了解更多有关离子指示剂(包括钙、钾、pH 值和膜电位指示剂)的信息›

钙指示剂(AM 酯)规格:
•标签(Ca2+– 结合形式的激发/发射波长):Fluo-4 (494/506 nm)
• 结合 Ca2+ 后荧光强度增加:>100 倍
• 缓冲液中 Ca2+ 的 Kd:∼335 nM
• 结合 Ca2+ 后,荧光增加,波长稍有变化


使用 TPEN 控制重金属阳离子
此外,基于 BAPTA 的指示剂可结合各种重金属阳离子(例如 Mn2+、Zn2+、Pb+2),亲和力远高于 Ca2+。可以使用重金属选择性螯合剂 TPEN 来控制由这些离子引起的钙测量值扰动。

荧光钙指示剂的更多选择
我们提供大量的 Molecular Probes™ 钙指示剂供各种实验场景选择使用。更多信息请参阅《Molecular Probes™ 手册》中的可见光激发的荧光 Ca2+ 指示剂—第 19.3 节

对于 UV 激发的 Ca2+ 指示剂、基于蛋白的 Ca2+ 指示剂、Ca2+ 指示剂的偶联物以及其他金属离子(即 Mg2+、Zn2+)的荧光指示剂,请查看 Molecular Probes™ 手册中的 Ca2+、Mg2+、Zn2+ 以及其他金属离子指示剂—第 19 章

仅供科研使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型基于荧光染料
数量5 x 1 mg
运输条件室温
适用于(应用)细胞活力、增殖与细胞成像
适用于(设备)共聚焦显微镜, 荧光显微镜, 高内涵分析仪, HTS 读数仪, 微孔板读数仪, 荧光成像仪
产品类型染料
Unit SizeEach
内容与储存
在 -5°C 至 -30°C 下避光储存。

常见问题解答 (FAQ)

Can I fix my cells after loading with Fluo-4 AM dye for the detection of calcium?

No. Since Fluo-4 AM isn't covalently bound to any cellular components and fixation compromises the membrane, the dye would not be retained by the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.