Fluo-5N, AM，细胞可透过性 - 特殊包装
Fluo-5N, AM，细胞可透过性 - 特殊包装
引用和文献 (15)
Invitrogen™

Fluo-5N, AM，细胞可透过性 - 特殊包装

标记钙指示剂是结合 Ca2+ 离子后荧光强度增加的分子。Fluo-5F、fluo-5N 和 fluo-4ff 是具有较低 Ca2+ 结合亲和力的了解更多信息
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货号数量
F1420410 x 50 μg
货号 F14204
价格（CNY)
6,704.00
Each
添加至购物车
数量:
10 x 50 μg
价格（CNY)
6,704.00
Each
添加至购物车
标记钙指示剂是结合 Ca2+ 离子后荧光强度增加的分子。Fluo-5F、fluo-5N 和 fluo-4ff 是具有较低 Ca2+ 结合亲和力的 fluo-4 类似物，使其适于检测 1 µM 至 1 mM 范围（将使 fluo-3 和 fluo-4 反应饱和）内的细胞内钙水平。将溶解后的指示剂直接加入含有培养细胞的培养皿中，即可向细胞上样 AM 酯形式的这类钙离子指示剂。这些指示剂与氩离子激光源在 488 nm 处的激发波长兼容，使其适用于共聚焦显微镜检查、流式细胞仪和微孔板筛选应用。

了解更多有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的信息 ›

钙指示剂（AM 酯）规格：
• 标签（Ca2+–结合形式的 Ex/Em）：Fluo-5N (494/516 nm)
• 结合 Ca2+ 后荧光强度增加：>100 倍
• 在缓冲液中与 Ca2+ 结合的 Kd：∼90 µM
• 结合 Ca2+ 后，荧光增加，波长稍有变化

使用 TPEN 控制重金属阳离子
另外，基于 BAPTA 的指示剂可结合各种重金属阳离子（例如 Mn2+、Zn2+、Pb2+），亲和力远高于 Ca2+。可以使用重金属选择性螯合剂 TPEN 来控制由这些离子引起的钙测量值扰动。

荧光钙指示剂的更多选择
我们提供大量的 Molecular Probes™ 钙指示剂供各种实验场景选择使用。更多信息请参阅《Molecular Probes™ 手册》中的可见光激发的荧光 Ca2+ 指示剂—第 19.3 节。

对于 UV 激发的 Ca2+ 指示剂、基于蛋白的 Ca2+ 指示剂、Ca2+ 指示剂的偶联物以及其他金属离子（即 Mg2+、Zn2+）的荧光指示剂，请查看 Molecular Probes™ 手册中的 Ca2+、Mg2+、Zn2+ 以及其他金属离子指示剂—第 19 章。

仅供科研使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型基于荧光染料
数量10 x 50 μg
运输条件室温
适用于（应用）细胞活力与增殖
适用于（设备）共聚焦显微镜, 荧光显微镜, 高内涵分析仪, HTS 读数仪, 微孔板读数仪, 荧光成像仪
产品类型钙指示剂
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (15)

引用和文献
Abstract
The use of the indicator fluo-5N to measure sarcoplasmic reticulum calcium in single muscle fibres of the cane toad.
Authors:Kabbara AA, Allen DG
Journal:J Physiol
PubMed ID:11432994
'1. Single fibres from the lumbrical muscles of the cane toad (Bufo marinus) were incubated in fluo-5N AM for 2 h at 35 degrees C in order to load the indicator into the sarcoplasmic reticulum. Fluo-5N is a low-affinity calcium indicator (K(Ca) 90 microM). Successful sarcoplasmic reticulum (SR) loading was ... More
Cardiac alternans do not rely on diastolic sarcoplasmic reticulum calcium content fluctuations.
Authors:Picht E, DeSantiago J, Blatter LA, Bers DM,
Journal:Circ Res
PubMed ID:16946134
'Cardiac alternans are thought to be a precursor to life-threatening arrhythmias. Previous studies suggested that alterations in sarcoplasmic reticulum (SR) Ca2+ content are either causative or not associated with myocyte Ca2+ alternans. However, those studies used indirect measures of SR Ca2+. Here we used direct continuous measurement of intra-SR free ... More
Kinesin dependent, rapid, bi-directional transport of ER sub-compartment in dendrites of hippocampal neurons.
Authors:Bannai H, Inoue T, Nakayama T, Hattori M, Mikoshiba K
Journal:J Cell Sci
PubMed ID:14676272
'Although spatially restricted Ca2+ release from the endoplasmic reticulum (ER) through intracellular Ca2+ channels plays important roles in various neuronal activities, the accurate distribution and dynamics of ER in the dendrite of living neurons still remain unknown. To elucidate these, we expressed fluorescent protein-tagged ER proteins in cultured mouse hippocampal ... More
Ca2+ blinks: rapid nanoscopic store calcium signaling.
Authors:Brochet DX, Yang D, Di Maio A, Lederer WJ, Franzini-Armstrong C, Cheng H
Journal:Proc Natl Acad Sci U S A
PubMed ID:15710901
'Luminal Ca(2+) in the endoplasmic and sarcoplasmic reticulum (ER/SR) plays an important role in regulating vital biological processes, including store-operated capacitative Ca(2+) entry, Ca(2+)-induced Ca(2+) release, and ER/SR stress-mediated cell death. We report rapid and substantial decreases in luminal [Ca(2+)], called "Ca(2+) blinks," within nanometer-sized stores (the junctional cisternae of ... More
Sulfhydryl oxidation overrides Mg(2+) inhibition of calcium-induced calcium release in skeletal muscle triads.
Authors:Donoso P, Aracena P, Hidalgo C
Journal:Biophys J
PubMed ID:10866954
'We studied the effect of oxidation of sulfhydryl (SH) residues on the inhibition by Mg(2+) of calcium-induced calcium release (CICR) in triad-enriched sarcoplasmic reticulum vesicles isolated from rabbit skeletal muscle. Vesicles were either passively or actively loaded with calcium before eliciting CICR by dilution at pCa 4.6-4.4 in the presence ... More
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