Fluo-4，AM，细胞可透过性

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引用和文献 (317)

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Fluo-4，AM，细胞可透过性

标记钙指示剂是结合 Ca2+ 离子后荧光强度增加的分子。Fluo-3 应用在流式细胞分析上，如涉及笼状螯合剂光活化、第二信使和神经递质的实验以及细胞水平的药理学筛选，用于 Ca2+ 的空间动力学成像。Fluo-4 是了解更多信息

货号	数量
F14217	500 μL

货号 F14217

价格（CNY)

3,539.00

Ends: 31-Dec-2025

4,766.00

共减 1,227.00 (26%)

添加至购物车

500 μL

价格（CNY)

3,539.00

Ends: 31-Dec-2025

4,766.00

共减 1,227.00 (26%)

添加至购物车

标记钙指示剂是结合 Ca²⁺ 离子后荧光强度增加的分子。Fluo-3 应用在流式细胞分析上，如涉及笼状螯合剂光活化、第二信使和神经递质的实验以及细胞水平的药理学筛选，用于 Ca²⁺ 的空间动力学成像。Fluo-4 是 fluo-3 的类似物，其中两个氯取代基被氟取代，导致在 488 nm 波长处的荧光激发增强，因此荧光信号水平更高。将溶解后的指示剂直接加入含有培养细胞的培养皿中，即可向细胞上样 AM 酯形式的这类钙离子指示剂。这些指示剂适用于荧光和共聚焦显微镜、流式细胞分析和微孔板筛选应用。

了解更多有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的信息›

钙指示剂（AM 酯）规格：
•标签（Ca²⁺– 结合形式的激发/发射波长）：Fluo-4 (494/506 nm)
• 结合 Ca²⁺ 后荧光强度增加：>100 倍
• 缓冲液中 Ca²⁺ 的 K_d：∼335 nM
• 结合 Ca²⁺ 后，荧光增加，波长稍有变化

使用 TPEN 控制重金属阳离子
此外，基于 BAPTA 的指示剂可结合各种重金属阳离子（例如 Mn²⁺、Zn²⁺、Pb⁺²），亲和力远高于 Ca²⁺。可以使用重金属选择性螯合剂 TPEN 来控制由这些离子引起的钙测量值扰动。

荧光钙指示剂的更多选择
我们提供大量的 Molecular Probes® 钙指示剂供各种实验场景选择使用。更多信息请参阅《Molecular Probes® 手册》中的可见光激发的荧光 Ca²⁺ 指示剂—第 19.3 节。

对于 UV 激发的 Ca²⁺ 指示剂、基于蛋白的 Ca²⁺ 指示剂、Ca²⁺ 指示剂的偶联物以及其他金属离子（即 Mg²⁺、Zn²⁺）的荧光指示剂，请查看 Molecular Probes® 手册中的 Ca²⁺、Mg²⁺、Zn²⁺ 以及其他金属离子指示剂—第 19 章。

仅供科研使用。不可用于人或动物的治疗或诊断。

仅供科研使用。不可用于诊断程序。

最大浓度1 mM

检测方法荧光

染料类型基于荧光染料

数量500 μL

运输条件室温

适用于（应用）细胞活力与增殖

适用于（设备）共聚焦显微镜, 荧光显微镜, 高内涵分析仪, HTS 读数仪, 微孔板读数仪, 荧光成像仪

产品类型染料

Unit SizeEach

内容与储存

在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

Can I fix my cells after loading with Fluo-4 AM dye for the detection of calcium?

No. Since Fluo-4 AM isn't covalently bound to any cellular components and fixation compromises the membrane, the dye would not be retained by the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The K_d value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (317)

引用和文献

Abstract

Functional implications of calcium permeability of the channel formed by pannexin 1.

Authors:Vanden Abeele F,Bidaux G,Gordienko D,Beck B,Panchin YV,Baranova AV,Ivanov DV,Skryma R,Prevarskaya N

Journal:The Journal of cell biology

PubMed ID:16908669

Although human pannexins (PanX) are homologous to gap junction molecules, their physiological function in vertebrates remains poorly understood. Our results demonstrate that overexpression of PanX1 results in the formation of Ca(2+)-permeable gap junction channels between adjacent cells, thus, allowing direct intercellular Ca(2+) diffusion and facilitating intercellular Ca(2+) wave propagation. More ... More

Impaired insulin secretion and glucose intolerance in synaptotagmin-7 null mutant mice.

Authors:Gustavsson N,Lao Y,Maximov A,Chuang JC,Kostromina E,Repa JJ,Li C,Radda GK,Südhof TC,Han W

Journal:Proceedings of the National Academy of Sciences of the United States of America

PubMed ID:18308938

Vertebrates express at least 15 different synaptotagmins with the same domain structure but diverse localizations and tissue distributions. Synaptotagmin-1,-2, and -9 act as calcium sensors for the fast phrase of neurotransmitter release, and synaptotagmin-12 acts as a calcium-independent modulator of release. The exact functions of the remaining 11 synaptotagmins, however, ... More

High-throughput microfluidic mixing and multiparametric cell sorting for bioactive compound screening.

Authors:Young SM, Curry MS, Ransom JT, Ballesteros JA, Prossnitz ER, Sklar LA, Edwards BS

Journal:J Biomol Screen

PubMed ID:15006133

HyperCyt, an automated sample handling system for flow cytometry that uses air bubbles to separate samples sequentially introduced from multiwell plates by an autosampler. In a previously documented HyperCyt configuration, air bubble separated compounds in one sample line and a continuous stream of cells in another are mixed in-line for ... More

Drosophila Hsc70-4 is critical for neurotransmitter exocytosis in vivo.

Authors:Bronk P, Wenniger JJ, Dawson-Scully K, Guo X, Hong S, Atwood HL, Zinsmaier KE

Journal:Neuron

PubMed ID:11395008

'Previous in vitro studies of cysteine-string protein (CSP) imply a potential role for the clathrin-uncoating ATPase Hsc70 in exocytosis. We show that hypomorphic mutations in Drosophila Hsc70-4 (Hsc4) impair nerve-evoked neurotransmitter release, but not synaptic vesicle recycling in vivo. The loss of release can be restored by increasing external or ... More

Multiplex GPCR assay in reverse transfection cell microarrays.

Authors:Mishina YM, Wilson CJ, Bruett L, Smith JJ, Stoop-Myer C, Jong S, Amaral LP, Pedersen R, Lyman SK, Myer VE, Kreider BL, Thompson CM

Journal:J Biomol Screen

PubMed ID:15140381

'G protein-coupled receptors (GPCRs) are a superfamily of proteins that include some of the most important drug targets in the pharmaceutical industry. Despite the success of this group of drugs, there remains a need to identify GPCR-targeted drugs with greater selectivity, to develop screening assays for validated targets, and to ... More

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